Mutagenetix > Incidental Mutation

Incidental Mutation 'R7686:Scart2'

593096

Institutional Source

Beutler Lab

Gene Symbol

Scart2

Ensembl Gene

ENSMUSG00000054672

Gene Name

scavenger receptor family member expressed on T cells 2

Synonyms

5830411N06Rik

MMRRC Submission

045751-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7686 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

139827197-139880649 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 139828965 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 208 (N208S)

Ref Sequence

ENSEMBL: ENSMUSP00000131905 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093984] [ENSMUST00000164583]

AlphaFold

B3F5L4

Predicted Effect

probably benign
Transcript: ENSMUST00000093984
AA Change: N208S

PolyPhen 2 Score 0.113 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000091520
Gene: ENSMUSG00000054672
AA Change: N208S

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SR	29	130	1.49e-18	SMART
SR	137	233	2.53e-4	SMART
SR	238	336	1.65e-34	SMART
SR	340	440	4.53e-32	SMART
SR	446	546	8.78e-30	SMART
SR	551	651	1.26e-53	SMART
SR	656	756	2.88e-16	SMART
SR	783	883	7.62e-48	SMART
transmembrane domain	903	925	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000164583
AA Change: N208S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000131905
Gene: ENSMUSG00000054672
AA Change: N208S

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SR	29	130	1.49e-18	SMART
SR	137	233	2.53e-4	SMART
Blast:SR	291	349	5e-12	BLAST
SR	354	452	1.65e-34	SMART
SR	456	556	4.53e-32	SMART
SR	562	662	8.78e-30	SMART
SR	667	767	1.26e-53	SMART
SR	772	872	2.88e-16	SMART
SR	899	999	7.62e-48	SMART
transmembrane domain	1019	1041	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (78/78)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acadl	A	T	1: 66,887,557 (GRCm39)		probably null	Het
Adgrg5	A	T	8: 95,664,430 (GRCm39)	I347F		Het
Agmo	T	C	12: 37,469,973 (GRCm39)	V431A	probably benign	Het
Arl14epl	A	G	18: 47,065,523 (GRCm39)	D96G	probably damaging	Het
Asxl1	C	T	2: 153,233,534 (GRCm39)	S113F	probably damaging	Het
Axdnd1	A	T	1: 156,223,034 (GRCm39)	C81*	probably null	Het
Bloc1s3	C	T	7: 19,241,531 (GRCm39)		probably benign	Het
Bmper	C	G	9: 23,310,840 (GRCm39)	L425V	probably benign	Het
Bok	A	T	1: 93,622,822 (GRCm39)	T194S	probably benign	Het
Bpifa5	A	T	2: 154,007,432 (GRCm39)	I125L	probably benign	Het
Brcc3dc	T	C	10: 108,535,301 (GRCm39)	E218G	probably damaging	Het
Brd10	A	T	19: 29,694,870 (GRCm39)	I1541N	probably benign	Het
Brpf3	T	C	17: 29,025,908 (GRCm39)	I327T	probably damaging	Het
Camk1	T	A	6: 113,313,158 (GRCm39)	N297Y	probably damaging	Het
Catsper2	C	T	2: 121,227,937 (GRCm39)		probably null	Het
Ccdc171	A	T	4: 83,575,556 (GRCm39)	N480I	unknown	Het
Cd83	A	G	13: 43,938,848 (GRCm39)	M22V	probably benign	Het
Col1a2	A	G	6: 4,518,964 (GRCm39)	D243G	unknown	Het
Crat	C	T	2: 30,294,577 (GRCm39)	R497Q	probably benign	Het
Ctla4	A	T	1: 60,951,752 (GRCm39)	T94S	probably benign	Het
Cyp2b19	T	C	7: 26,461,768 (GRCm39)	F196S	probably damaging	Het
Dhx32	A	T	7: 133,361,430 (GRCm39)	M1K	probably null	Het
Epha3	T	A	16: 63,593,651 (GRCm39)	I146F	probably damaging	Het
Erbb2	T	A	11: 98,326,399 (GRCm39)	S1008T	probably benign	Het
Esp34	A	G	17: 38,870,534 (GRCm39)	R143G	possibly damaging	Het
Fam135b	T	C	15: 71,335,233 (GRCm39)	K654E	possibly damaging	Het
Fgr	T	C	4: 132,725,324 (GRCm39)	M343T	probably benign	Het
Fibcd1	T	G	2: 31,723,880 (GRCm39)	D253A	probably damaging	Het
Foxk1	C	T	5: 142,387,625 (GRCm39)	S116L	probably damaging	Het
Gimap8	G	A	6: 48,633,006 (GRCm39)	G275E	probably damaging	Het
Glp1r	T	C	17: 31,144,633 (GRCm39)	Y269H	probably damaging	Het
H2ac20	T	C	3: 96,127,886 (GRCm39)	E92G	probably damaging	Het
Lbr	A	G	1: 181,645,086 (GRCm39)	F522S	probably damaging	Het
Lca5	T	C	9: 83,277,292 (GRCm39)	N684S	probably benign	Het
Leng8	C	A	7: 4,146,504 (GRCm39)	S372*	probably null	Het
Lrp2	A	G	2: 69,319,581 (GRCm39)	Y1981H	probably damaging	Het
Lrrc37a	T	G	11: 103,389,062 (GRCm39)	Q2121P	unknown	Het
Lrrn2	A	G	1: 132,866,332 (GRCm39)	T466A	probably benign	Het
Map3k19	A	T	1: 127,749,985 (GRCm39)	L1122*	probably null	Het
Mfsd6	T	C	1: 52,701,554 (GRCm39)	E632G	probably benign	Het
Mfsd6l	C	T	11: 68,448,052 (GRCm39)	A301V	probably benign	Het
Mro	A	T	18: 74,010,510 (GRCm39)	M197L	probably benign	Het
Ncam2	A	T	16: 81,418,342 (GRCm39)	E799V	probably damaging	Het
Nek9	A	T	12: 85,350,433 (GRCm39)	D880E	probably benign	Het
Ntng1	T	A	3: 109,842,330 (GRCm39)	I148L	possibly damaging	Het
Or1ad6	A	G	11: 50,860,582 (GRCm39)	T246A	possibly damaging	Het
Or5ac23	T	A	16: 59,149,379 (GRCm39)	R164S	probably damaging	Het
P2ry13	C	T	3: 59,117,439 (GRCm39)	G113D	probably damaging	Het
Pnpt1	A	T	11: 29,107,070 (GRCm39)	T687S	probably damaging	Het
Pou2af1	T	C	9: 51,149,486 (GRCm39)	Y193H	probably damaging	Het
Ppp1r14c	T	A	10: 3,413,396 (GRCm39)	I143N	probably damaging	Het
Ppp1r16a	A	T	15: 76,578,783 (GRCm39)	S496C	probably benign	Het
Prdm12	G	A	2: 31,530,229 (GRCm39)	R40Q	probably damaging	Het
Rabepk	C	T	2: 34,669,308 (GRCm39)	G362S	probably damaging	Het
Rftn1	G	T	17: 50,354,408 (GRCm39)	A318D	probably damaging	Het
Rnf26rt	A	T	6: 76,474,013 (GRCm39)	L201H	probably damaging	Het
Robo2	C	A	16: 73,755,293 (GRCm39)	V743F	probably damaging	Het
Scart1	T	A	7: 139,802,116 (GRCm39)	C148*	probably null	Het
Smg1	A	T	7: 117,767,081 (GRCm39)	Y1804N	unknown	Het
Ssh2	A	G	11: 77,316,150 (GRCm39)	E257G	possibly damaging	Het
Stoml3	T	C	3: 53,410,589 (GRCm39)	I101T	probably damaging	Het
Stxbp5	T	C	10: 9,645,154 (GRCm39)	D950G	probably damaging	Het
Syt10	T	C	15: 89,698,360 (GRCm39)	D328G	probably damaging	Het
Taf3	T	C	2: 9,956,299 (GRCm39)	K623E	probably damaging	Het
Tas2r134	T	A	2: 51,518,255 (GRCm39)	S245T	possibly damaging	Het
Tfap2b	G	C	1: 19,284,511 (GRCm39)	D140H	probably damaging	Het
Tjp3	C	T	10: 81,113,885 (GRCm39)	V458I	probably benign	Het
Tnpo3	G	T	6: 29,562,899 (GRCm39)	S606*	probably null	Het
Tram1l1	T	C	3: 124,114,959 (GRCm39)	F40L	probably benign	Het
Ttc29	A	T	8: 79,119,759 (GRCm39)	T464S	probably benign	Het
Txndc9	A	T	1: 38,026,849 (GRCm39)	M192K	probably benign	Het
Urb2	C	A	8: 124,771,911 (GRCm39)	A1444E	probably benign	Het
Uty	G	A	Y: 1,158,075 (GRCm39)	T657I	possibly damaging	Het
Vmn1r1	A	T	1: 181,985,590 (GRCm39)	V25D	probably damaging	Het
Vmn2r65	A	T	7: 84,589,744 (GRCm39)	I724N	probably damaging	Het
Xkr6	C	T	14: 63,844,295 (GRCm39)	T106M	possibly damaging	Het
Zc3h18	G	A	8: 123,140,615 (GRCm39)	R850Q	unknown	Het
Zfp341	A	G	2: 154,466,781 (GRCm39)	E50G	probably damaging	Het
Zfp62	C	A	11: 49,107,985 (GRCm39)	A692E	probably damaging	Het

Other mutations in Scart2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Scart2	APN	7	139,874,755 (GRCm39)	missense	probably damaging	0.99
IGL01101:Scart2	APN	7	139,876,017 (GRCm39)	missense	probably benign	0.35
IGL01120:Scart2	APN	7	139,876,472 (GRCm39)	missense	probably benign	0.02
IGL01958:Scart2	APN	7	139,854,040 (GRCm39)	missense	probably damaging	1.00
IGL02150:Scart2	APN	7	139,877,772 (GRCm39)	missense	possibly damaging	0.84
IGL02193:Scart2	APN	7	139,828,913 (GRCm39)	missense	probably benign	0.17
IGL02239:Scart2	APN	7	139,875,756 (GRCm39)	missense	probably damaging	1.00
IGL02335:Scart2	APN	7	139,876,453 (GRCm39)	missense	probably damaging	1.00
IGL02569:Scart2	APN	7	139,878,275 (GRCm39)	missense	probably benign	0.01
IGL02993:Scart2	APN	7	139,876,486 (GRCm39)	missense	probably benign	0.07
IGL03261:Scart2	APN	7	139,874,746 (GRCm39)	missense	probably benign	0.00
IGL03365:Scart2	APN	7	139,876,682 (GRCm39)	missense	probably damaging	1.00
IGL03399:Scart2	APN	7	139,827,869 (GRCm39)	missense	probably benign	0.00
IGL03052:Scart2	UTSW	7	139,828,827 (GRCm39)	missense	probably damaging	1.00
PIT4791001:Scart2	UTSW	7	139,853,975 (GRCm39)	missense	possibly damaging	0.53
R0021:Scart2	UTSW	7	139,876,310 (GRCm39)	missense	probably benign	0.15
R0021:Scart2	UTSW	7	139,876,310 (GRCm39)	missense	probably benign	0.15
R0347:Scart2	UTSW	7	139,877,767 (GRCm39)	missense	probably damaging	1.00
R0374:Scart2	UTSW	7	139,828,874 (GRCm39)	missense	probably damaging	1.00
R0639:Scart2	UTSW	7	139,827,872 (GRCm39)	missense	probably benign	0.01
R0667:Scart2	UTSW	7	139,841,450 (GRCm39)	missense	possibly damaging	0.73
R0789:Scart2	UTSW	7	139,828,133 (GRCm39)	missense	probably damaging	1.00
R0959:Scart2	UTSW	7	139,874,704 (GRCm39)	missense	probably damaging	1.00
R1316:Scart2	UTSW	7	139,879,583 (GRCm39)	missense	probably benign	0.09
R1764:Scart2	UTSW	7	139,877,178 (GRCm39)	missense	probably benign	0.00
R2247:Scart2	UTSW	7	139,829,042 (GRCm39)	missense	probably null	0.96
R2379:Scart2	UTSW	7	139,879,682 (GRCm39)	missense	probably benign	0.15
R4112:Scart2	UTSW	7	139,878,281 (GRCm39)	nonsense	probably null
R4114:Scart2	UTSW	7	139,877,823 (GRCm39)	missense	probably damaging	1.00
R4346:Scart2	UTSW	7	139,827,878 (GRCm39)	missense	probably damaging	0.97
R4836:Scart2	UTSW	7	139,879,021 (GRCm39)	missense	probably benign
R4956:Scart2	UTSW	7	139,878,275 (GRCm39)	missense	probably benign	0.00
R5208:Scart2	UTSW	7	139,877,949 (GRCm39)	missense	probably benign	0.00
R5571:Scart2	UTSW	7	139,829,036 (GRCm39)	missense	probably damaging	1.00
R5583:Scart2	UTSW	7	139,876,739 (GRCm39)	missense	probably damaging	1.00
R5645:Scart2	UTSW	7	139,828,853 (GRCm39)	missense	possibly damaging	0.95
R6183:Scart2	UTSW	7	139,875,947 (GRCm39)	missense	possibly damaging	0.82
R6995:Scart2	UTSW	7	139,841,514 (GRCm39)	missense	probably benign
R7436:Scart2	UTSW	7	139,841,520 (GRCm39)	missense	probably benign
R7621:Scart2	UTSW	7	139,876,742 (GRCm39)	missense	probably damaging	1.00
R7662:Scart2	UTSW	7	139,874,725 (GRCm39)	missense	possibly damaging	0.58
R7669:Scart2	UTSW	7	139,876,234 (GRCm39)	missense	possibly damaging	0.47
R7985:Scart2	UTSW	7	139,876,806 (GRCm39)	missense	probably damaging	1.00
R8330:Scart2	UTSW	7	139,876,231 (GRCm39)	nonsense	probably null
R8843:Scart2	UTSW	7	139,828,913 (GRCm39)	missense	possibly damaging	0.93
R8888:Scart2	UTSW	7	139,841,532 (GRCm39)	missense	possibly damaging	0.93
R8895:Scart2	UTSW	7	139,841,532 (GRCm39)	missense	possibly damaging	0.93
R9044:Scart2	UTSW	7	139,828,010 (GRCm39)	missense	probably damaging	1.00
R9142:Scart2	UTSW	7	139,877,806 (GRCm39)	missense	probably damaging	1.00
R9152:Scart2	UTSW	7	139,877,256 (GRCm39)	missense	possibly damaging	0.55
R9470:Scart2	UTSW	7	139,827,345 (GRCm39)	missense	probably benign	0.07
R9509:Scart2	UTSW	7	139,879,644 (GRCm39)	nonsense	probably null
R9522:Scart2	UTSW	7	139,853,987 (GRCm39)	missense	possibly damaging	0.73
R9755:Scart2	UTSW	7	139,841,544 (GRCm39)	critical splice donor site	probably null
R9794:Scart2	UTSW	7	139,874,716 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTAGTTGATGGTGGCAGTCC -3'
(R):5'- AACTATGAATCTGGGGTAAGGGTTC -3'

Sequencing Primer
(F):5'- ATGGTGGCAGTCCTTGTGC -3'
(R):5'- GGGTAAGGGTTCTATTGACACTAC -3'

Posted On

2019-11-12