Mutagenetix > Incidental Mutation

Incidental Mutation 'R7687:Mslnl'

593180

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7687 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 25743183 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 185 (V185A)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V185A

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V185A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	T	C	1: 71,258,182 (GRCm38)	K2383R	probably benign	Het
Acly	C	T	11: 100,504,854 (GRCm38)		probably null	Het
Baiap3	T	A	17: 25,249,337 (GRCm38)	I276F	possibly damaging	Het
Cdc14b	A	T	13: 64,209,193 (GRCm38)	D419E	probably benign	Het
Celsr2	G	T	3: 108,397,769 (GRCm38)	P2165T	probably benign	Het
Clk4	A	G	11: 51,281,398 (GRCm38)	D476G	probably benign	Het
Dera	A	G	6: 137,836,880 (GRCm38)	T10A		Het
Dip2c	A	T	13: 9,604,581 (GRCm38)	T742S	probably benign	Het
Dohh	C	A	10: 81,387,806 (GRCm38)	A231E	probably benign	Het
Dot1l	T	G	10: 80,789,368 (GRCm38)	S1150A	possibly damaging	Het
Eea1	T	A	10: 96,026,598 (GRCm38)	I794N	probably benign	Het
En2	T	C	5: 28,170,289 (GRCm38)	S277P	probably damaging	Het
Erich1	A	G	8: 14,030,691 (GRCm38)	L276P	probably damaging	Het
Flnb	A	G	14: 7,924,224 (GRCm38)	N1779S	probably damaging	Het
Frzb	T	A	2: 80,424,635 (GRCm38)	T186S	probably benign	Het
Gdf7	T	A	12: 8,298,257 (GRCm38)	R347*	probably null	Het
Ighv9-4	T	C	12: 114,300,263 (GRCm38)	I17V	not run	Het
Ipo13	G	A	4: 117,911,891 (GRCm38)	P235S	probably benign	Het
Itga2	C	A	13: 114,866,260 (GRCm38)	G565C	probably damaging	Het
Kcnc4	CCCGCCGCCGCCGCCGCCGCCGC	CCCGCCGCCGCCGCCGCCGCCGCCGC	3: 107,458,609 (GRCm38)		probably benign	Het
Kcnk10	A	G	12: 98,435,096 (GRCm38)	I440T	probably damaging	Het
Kdm3a	T	A	6: 71,599,492 (GRCm38)	K779N	possibly damaging	Het
Kmt2d	C	T	15: 98,862,120 (GRCm38)	D1086N	unknown	Het
Kntc1	A	G	5: 123,759,089 (GRCm38)	I172V	probably benign	Het
Maip1	A	G	1: 57,411,844 (GRCm38)	E215G	probably damaging	Het
Mms19	A	T	19: 41,955,168 (GRCm38)	M417K	possibly damaging	Het
Naa11	A	T	5: 97,391,789 (GRCm38)	V170E	probably benign	Het
Ncapg	C	T	5: 45,699,885 (GRCm38)	P980S	probably benign	Het
Olfr170	T	C	16: 19,605,735 (GRCm38)	N310S	probably benign	Het
Pbxip1	A	G	3: 89,448,199 (GRCm38)	D675G	probably damaging	Het
Pdlim5	A	G	3: 142,277,847 (GRCm38)	S382P	probably benign	Het
Pkd1l1	T	A	11: 8,854,390 (GRCm38)	I2184F		Het
Plau	A	G	14: 20,839,798 (GRCm38)	Y237C	probably damaging	Het
Ppl	T	C	16: 5,097,942 (GRCm38)	T586A	probably benign	Het
Rapgef6	T	G	11: 54,661,075 (GRCm38)	I923S	possibly damaging	Het
Rbfox2	C	T	15: 77,306,494 (GRCm38)	G17D	unknown	Het
Sema3b	T	C	9: 107,603,814 (GRCm38)	D108G	probably damaging	Het
Slc6a20a	A	G	9: 123,656,266 (GRCm38)	I297T	probably damaging	Het
Slit1	A	G	19: 41,650,689 (GRCm38)	F261L	probably benign	Het
Tcp10a	T	A	17: 7,345,108 (GRCm38)	V433D	probably damaging	Het
Tktl2	A	G	8: 66,513,101 (GRCm38)	E437G	probably damaging	Het
Tll1	G	T	8: 64,121,492 (GRCm38)	Y109*	probably null	Het
Tmem79	A	G	3: 88,332,581 (GRCm38)	V274A	probably damaging	Het
Tnfrsf23	G	A	7: 143,681,462 (GRCm38)	S55L	probably benign	Het
Ubd	T	C	17: 37,193,974 (GRCm38)		probably null	Het
Ubl3	C	A	5: 148,506,175 (GRCm38)	R105L	possibly damaging	Het
Ubl7	A	T	9: 57,914,584 (GRCm38)	D72V	probably damaging	Het
Wdr55	T	C	18: 36,762,023 (GRCm38)	S81P	probably damaging	Het
Wtip	T	C	7: 34,116,619 (GRCm38)	Y344C	probably damaging	Het
Zfp488	A	G	14: 33,970,400 (GRCm38)	S269P	possibly damaging	Het
Zkscan2	A	C	7: 123,499,862 (GRCm38)	S36A	probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,743,159 (GRCm38)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- AACTTGCCCAACCAGAGAGTTG -3'
(R):5'- TTCAAACCTCAAGCCCCTTG -3'

Sequencing Primer
(F):5'- AACCAGAGAGTTGCCCTGCAG -3'
(R):5'- AAGCCCCTTGCATCTTATCTGATG -3'

Posted On

2019-11-12