Mutagenetix > Incidental Mutation

Incidental Mutation 'R7692:Lzts3'

593437

Institutional Source

Beutler Lab

Gene Symbol

Lzts3

Ensembl Gene

ENSMUSG00000037703

Gene Name

leucine zipper, putative tumor suppressor family member 3

Synonyms

Prosapip1

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.200) question?

Stock #

R7692 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130632839-130642803 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 130635386 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 381 (S381G)

Ref Sequence

ENSEMBL: ENSMUSP00000105889 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045761] [ENSMUST00000089561] [ENSMUST00000110260]

AlphaFold

A2AHG0

Predicted Effect

probably benign
Transcript: ENSMUST00000045761
AA Change: S495G

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000037109
Gene: ENSMUSG00000037703
AA Change: S495G

Domain	Start	End	E-Value	Type
low complexity region	86	107	N/A	INTRINSIC
low complexity region	147	158	N/A	INTRINSIC
low complexity region	240	250	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	285	297	N/A	INTRINSIC
low complexity region	301	333	N/A	INTRINSIC
low complexity region	337	346	N/A	INTRINSIC
low complexity region	349	361	N/A	INTRINSIC
low complexity region	402	436	N/A	INTRINSIC
Pfam:Fez1	465	665	1.4e-62	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000089561
AA Change: S495G

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000086990
Gene: ENSMUSG00000037703
AA Change: S495G

Domain	Start	End	E-Value	Type
low complexity region	86	107	N/A	INTRINSIC
low complexity region	147	158	N/A	INTRINSIC
low complexity region	240	250	N/A	INTRINSIC
low complexity region	253	265	N/A	INTRINSIC
low complexity region	285	297	N/A	INTRINSIC
low complexity region	301	333	N/A	INTRINSIC
low complexity region	337	346	N/A	INTRINSIC
low complexity region	349	361	N/A	INTRINSIC
low complexity region	402	436	N/A	INTRINSIC
Pfam:Fez1	465	666	2.1e-74	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000110260
AA Change: S381G

PolyPhen 2 Score 0.271 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000105889
Gene: ENSMUSG00000037703
AA Change: S381G

Domain	Start	End	E-Value	Type
low complexity region	126	136	N/A	INTRINSIC
low complexity region	139	151	N/A	INTRINSIC
low complexity region	171	183	N/A	INTRINSIC
low complexity region	187	219	N/A	INTRINSIC
low complexity region	223	232	N/A	INTRINSIC
low complexity region	235	247	N/A	INTRINSIC
low complexity region	288	322	N/A	INTRINSIC
Pfam:Fez1	351	552	1.5e-74	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam26b	G	A	8: 43,520,795	T390I	probably benign	Het
Adgb	A	G	10: 10,411,712		probably null	Het
Ado	G	T	10: 67,548,435	Y113*	probably null	Het
Angptl1	A	G	1: 156,845,315	E237G	probably damaging	Het
Arid2	T	A	15: 96,356,697	Y141*	probably null	Het
Clvs1	T	C	4: 9,350,739	I183T	probably benign	Het
Dnah1	T	C	14: 31,292,338	K1817E	probably benign	Het
Eif3e	C	A	15: 43,263,246	R271L	probably damaging	Het
Eml6	A	G	11: 29,753,085	V1611A	probably damaging	Het
Enpp3	G	T	10: 24,784,841	Y634*	probably null	Het
Evi5l	C	T	8: 4,200,886	R394W	probably damaging	Het
Fcgr3	A	C	1: 171,054,092	F156V	probably damaging	Het
Fmo1	T	A	1: 162,833,833	T294S	probably benign	Het
Gabrb3	A	G	7: 57,816,455	Q339R	probably damaging	Het
Gen1	T	A	12: 11,242,166	T606S	probably benign	Het
Gfod1	T	C	13: 43,201,052	Q149R	probably benign	Het
Golm1	A	G	13: 59,640,257	V276A	probably benign	Het
Hc	C	A	2: 35,024,149	V849F	probably damaging	Het
Hectd4	T	C	5: 121,321,564	I832T	possibly damaging	Het
Hspa14	T	C	2: 3,496,606	D283G	probably damaging	Het
Lztfl1	C	T	9: 123,712,471	W94*	probably null	Het
Mgat2	A	G	12: 69,184,670	Y6C	probably damaging	Het
Mrpl30	T	C	1: 37,895,358	I27T	probably benign	Het
Muc5b	A	T	7: 141,853,229	T1045S	unknown	Het
Nlrp4a	G	A	7: 26,449,265	R99Q	probably benign	Het
Olfr1450	A	G	19: 12,953,642	T18A	possibly damaging	Het
Olfr1510	T	C	14: 52,410,488	Y128C	probably damaging	Het
Phlpp1	T	C	1: 106,281,402	L495P	probably damaging	Het
Pla2g4d	T	C	2: 120,279,295	D178G	possibly damaging	Het
Prl5a1	C	G	13: 28,150,014	L167V	probably damaging	Het
Sardh	A	G	2: 27,197,639	V740A	probably benign	Het
Sbno1	T	G	5: 124,405,646	T277P	probably benign	Het
Slc2a1	T	C	4: 119,136,265	V433A	probably damaging	Het
Slc4a10	T	C	2: 62,303,964	V1008A	possibly damaging	Het
Smarcal1	T	G	1: 72,586,020	S109A	probably benign	Het
Speg	A	G	1: 75,401,190	D864G	probably benign	Het
Steap4	T	C	5: 7,976,976	I313T	probably benign	Het
Tab2	A	T	10: 7,911,105	D614E	probably damaging	Het
Tenm4	A	G	7: 96,895,403	K2246E	probably damaging	Het
Timm23	A	G	14: 32,180,563	S208P	probably damaging	Het
Tmem229a	C	A	6: 24,955,212	C181F	probably benign	Het
Ugdh	T	C	5: 65,417,615	Y356C	probably damaging	Het
Ugt2a1	A	G	5: 87,486,727	L7P	probably damaging	Het
Uhrf1	A	G	17: 56,312,905	D272G	possibly damaging	Het
Vmn1r206	T	A	13: 22,620,657	I127F	probably damaging	Het
Vmn2r106	T	C	17: 20,285,228	Y68C	possibly damaging	Het
Zfand6	G	A	7: 84,633,933	P72L	not run	Het

Other mutations in Lzts3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01072:Lzts3	APN	2	130635445	missense	probably damaging	0.99
IGL01541:Lzts3	APN	2	130636206	missense	probably damaging	0.99
IGL01649:Lzts3	APN	2	130635431	missense	probably damaging	0.98
IGL02746:Lzts3	APN	2	130636341	missense	probably damaging	0.98
IGL02927:Lzts3	APN	2	130637957	utr 5 prime	probably benign
R0069:Lzts3	UTSW	2	130636540	missense	probably benign	0.16
R0069:Lzts3	UTSW	2	130636540	missense	probably benign	0.16
R0173:Lzts3	UTSW	2	130634768	makesense	probably null
R1381:Lzts3	UTSW	2	130635299	missense	probably damaging	0.99
R4127:Lzts3	UTSW	2	130635365	missense	probably damaging	0.99
R4301:Lzts3	UTSW	2	130636438	missense	probably damaging	0.99
R4588:Lzts3	UTSW	2	130634766	makesense	probably null
R5289:Lzts3	UTSW	2	130636101	missense	probably benign	0.18
R5878:Lzts3	UTSW	2	130636539	missense	probably damaging	1.00
R5964:Lzts3	UTSW	2	130636288	missense	probably damaging	0.99
R6193:Lzts3	UTSW	2	130637386	missense	probably damaging	0.97
R8195:Lzts3	UTSW	2	130635185	missense	probably benign	0.01
R8273:Lzts3	UTSW	2	130634881	missense	possibly damaging	0.66
R9126:Lzts3	UTSW	2	130635328	missense	possibly damaging	0.66
R9129:Lzts3	UTSW	2	130634945	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- GCTGGAGCTGTTTCTGATACTC -3'
(R):5'- CCCTATGAGTGCTTAGTGCC -3'

Sequencing Primer
(F):5'- GGAGCTGTTTCTGATACTCAATGACC -3'
(R):5'- CCTTTGGAGATAGATCTGGGAGC -3'

Posted On

2019-11-12