Mutagenetix > Incidental Mutation

Incidental Mutation 'R7699:Cubn'

593827

Institutional Source

Beutler Lab

Gene Symbol

Cubn

Ensembl Gene

ENSMUSG00000026726

Gene Name

cubilin (intrinsic factor-cobalamin receptor)

Synonyms

D2Wsu88e

MMRRC Submission

045760-MU

Accession Numbers

Genbank: NM_001081084; MGI: 1931256

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7699 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

13276338-13491813 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 13489917 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 107 (V107A)

Ref Sequence

ENSEMBL: ENSMUSP00000089009 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091436]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000091436
AA Change: V107A

PolyPhen 2 Score 0.735 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000089009
Gene: ENSMUSG00000026726
AA Change: V107A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
EGF	132	165	2.14e-5	SMART
EGF_CA	167	208	1.95e-8	SMART
EGF	213	258	2.85e-1	SMART
EGF_CA	260	301	2.66e-10	SMART
EGF_CA	302	345	7.07e-6	SMART
EGF	349	393	1.01e-1	SMART
EGF	398	430	3.73e-5	SMART
EGF_CA	432	468	8.63e-10	SMART
CUB	474	586	4.4e-21	SMART
CUB	590	702	3.82e-39	SMART
CUB	708	816	3.66e-18	SMART
CUB	817	928	3.09e-25	SMART
CUB	932	1042	1.29e-36	SMART
CUB	1048	1161	3.46e-37	SMART
CUB	1165	1277	7.24e-40	SMART
CUB	1278	1389	8.33e-31	SMART
CUB	1391	1506	3.08e-43	SMART
CUB	1510	1619	1.9e-34	SMART
CUB	1620	1734	7.24e-40	SMART
CUB	1738	1850	6.02e-37	SMART
CUB	1852	1963	1.57e-26	SMART
CUB	1978	2091	3.46e-28	SMART
CUB	2092	2213	2.88e-34	SMART
CUB	2217	2334	4.13e-35	SMART
CUB	2336	2448	3.1e-39	SMART
CUB	2452	2565	5.37e-34	SMART
CUB	2570	2687	3e-23	SMART
CUB	2689	2801	3.1e-39	SMART
CUB	2805	2919	2.36e-21	SMART
CUB	2920	3035	6.18e-25	SMART
CUB	3037	3150	5.16e-36	SMART
CUB	3157	3274	1.68e-35	SMART
CUB	3278	3393	7.17e-12	SMART
CUB	3395	3507	2.49e-29	SMART
CUB	3511	3623	2.4e-22	SMART

Meta Mutation Damage Score

0.1712

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null mutation display embryonic lethality during organogenesis, yolk sac and allantoic vasculature defects, embryonic and visceral endoderm defects, and lack somites. Heterozygotes display incomplete penetrance of premature death. [provided by MGI curators]

Allele List at MGI

All alleles(4) : Targeted, other(1) Gene trapped(3)

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810009A15Rik	T	C	19: 8,890,053 (GRCm38)	L72P	probably benign	Het
4930407I10Rik	C	A	15: 82,064,105 (GRCm38)	H734Q	probably benign	Het
4932438A13Rik	T	A	3: 36,974,172 (GRCm38)	N2329K	possibly damaging	Het
4932438A13Rik	C	T	3: 37,026,154 (GRCm38)	S267L	probably benign	Het
Acadl	A	G	1: 66,838,363 (GRCm38)	V343A	possibly damaging	Het
Acot4	A	T	12: 84,043,237 (GRCm38)	D236V	probably damaging	Het
App	T	A	16: 85,040,309 (GRCm38)		probably null	Het
Arfgef1	G	A	1: 10,194,411 (GRCm38)	T470I	possibly damaging	Het
Arhgef5	T	C	6: 43,274,757 (GRCm38)	I814T	probably benign	Het
Atr	A	T	9: 95,875,690 (GRCm38)	R968*	probably null	Het
C130060K24Rik	T	A	6: 65,452,956 (GRCm38)	I212N	probably benign	Het
Capn7	C	T	14: 31,352,444 (GRCm38)	T268I	probably benign	Het
Cbwd1	A	G	19: 24,942,681 (GRCm38)		probably null	Het
Cyp11b1	T	A	15: 74,835,842 (GRCm38)	T473S	probably damaging	Het
Cyr61	C	T	3: 145,648,692 (GRCm38)	G155R	probably damaging	Het
Dip2c	T	G	13: 9,659,311 (GRCm38)	S1396A	probably benign	Het
Dyrk1b	T	C	7: 28,184,312 (GRCm38)	Y198H	probably damaging	Het
Echdc2	G	T	4: 108,174,077 (GRCm38)	R206L	probably benign	Het
Ep400	C	T	5: 110,696,032 (GRCm38)	R1594Q	unknown	Het
Fmnl2	G	A	2: 53,036,508 (GRCm38)	R69Q		Het
Fry	T	A	5: 150,405,327 (GRCm38)	H1308Q	probably damaging	Het
Fyb2	T	A	4: 105,010,454 (GRCm38)	D667E	probably benign	Het
Gimap7	T	A	6: 48,723,857 (GRCm38)	Y126N	possibly damaging	Het
Gm1979	A	T	5: 26,000,180 (GRCm38)	W266R	probably damaging	Het
Gm28042	T	A	2: 120,039,716 (GRCm38)	M712K	possibly damaging	Het
Gm3238	A	G	10: 77,770,635 (GRCm38)	*231R	probably null	Het
Gm5592	C	A	7: 41,286,407 (GRCm38)	A111E	probably damaging	Het
Gm7694	A	G	1: 170,301,148 (GRCm38)	*271Q	probably null	Het
Ift81	T	C	5: 122,594,560 (GRCm38)	M304V	possibly damaging	Het
Igfals	T	C	17: 24,880,574 (GRCm38)	L213P	probably damaging	Het
Ints3	G	A	3: 90,421,804 (GRCm38)	P83S	probably benign	Het
Kcna10	T	G	3: 107,195,540 (GRCm38)	S496A	probably damaging	Het
Kmt2d	G	A	15: 98,843,719 (GRCm38)	A4520V	unknown	Het
Lemd3	A	T	10: 120,978,090 (GRCm38)	Y413N	probably damaging	Het
Lgr6	T	C	1: 134,996,032 (GRCm38)	N453S	probably damaging	Het
Lmtk3	A	C	7: 45,792,574 (GRCm38)	D352A	probably damaging	Het
Lrrc4c	T	C	2: 97,630,679 (GRCm38)	M550T	possibly damaging	Het
Maml2	A	T	9: 13,621,089 (GRCm38)	Q533L		Het
Mdn1	T	A	4: 32,741,344 (GRCm38)	D3815E	probably damaging	Het
Megf8	C	T	7: 25,329,928 (GRCm38)	A299V	possibly damaging	Het
Meis3	G	T	7: 16,177,556 (GRCm38)	E59D	probably benign	Het
Mppe1	A	G	18: 67,225,704 (GRCm38)	*398R	probably null	Het
Mtmr7	G	A	8: 40,606,884 (GRCm38)	A62V	possibly damaging	Het
Mtus1	T	A	8: 41,083,969 (GRCm38)	T237S	possibly damaging	Het
Ndfip2	T	A	14: 105,287,759 (GRCm38)	V158E	possibly damaging	Het
Nrbp2	T	G	15: 76,090,897 (GRCm38)	T53P	probably damaging	Het
Nrde2	G	A	12: 100,130,835 (GRCm38)	P902L	probably benign	Het
Olfr1383	A	G	11: 49,524,554 (GRCm38)	Y277C	probably damaging	Het
Olfr509	T	A	7: 108,645,672 (GRCm38)	K301N	probably damaging	Het
Olfr642	T	A	7: 104,050,593 (GRCm38)		probably benign	Het
Olfr739	T	A	14: 50,425,335 (GRCm38)	M272K	probably benign	Het
Olfr771	T	C	10: 129,160,055 (GRCm38)	M310V	probably benign	Het
Pcdha1	A	G	18: 36,931,062 (GRCm38)	T260A	probably damaging	Het
Pdlim1	T	G	19: 40,249,658 (GRCm38)	K98N	probably damaging	Het
Phrf1	G	A	7: 141,254,929 (GRCm38)	G207R	unknown	Het
Pik3r6	A	G	11: 68,528,563 (GRCm38)	K124R	probably damaging	Het
Polr2b	T	A	5: 77,340,421 (GRCm38)	F823I	probably benign	Het
Prss58	T	C	6: 40,895,388 (GRCm38)	I234V	probably damaging	Het
Ptpn14	A	T	1: 189,865,411 (GRCm38)	N766I	probably benign	Het
Repin1	T	A	6: 48,597,822 (GRCm38)	S562T	probably damaging	Het
Rgr	C	T	14: 37,044,595 (GRCm38)	D165N	probably damaging	Het
Rho	T	C	6: 115,935,239 (GRCm38)	F221L	probably damaging	Het
Rictor	C	T	15: 6,772,154 (GRCm38)	S441L	probably benign	Het
Rnase2a	A	G	14: 51,255,791 (GRCm38)	I39T	probably damaging	Het
Rpl13a	T	G	7: 45,127,236 (GRCm38)	I43L	probably benign	Het
Rpp30	T	C	19: 36,089,158 (GRCm38)	V97A	probably benign	Het
Scube3	T	C	17: 28,167,049 (GRCm38)	Y755H	probably damaging	Het
Sec24a	A	T	11: 51,712,257 (GRCm38)	V788E	probably damaging	Het
Spns2	A	T	11: 72,489,617 (GRCm38)	L60*	probably null	Het
Strc	T	C	2: 121,371,748 (GRCm38)	N1213S	possibly damaging	Het
Tec	A	T	5: 72,786,024 (GRCm38)	I116N	possibly damaging	Het
Tll1	A	T	8: 64,093,954 (GRCm38)	D319E	probably benign	Het
Tmem121b	T	C	6: 120,493,427 (GRCm38)	T110A	unknown	Het
Tmem232	T	A	17: 65,265,218 (GRCm38)	I593F	probably damaging	Het
Tpp2	A	G	1: 43,970,466 (GRCm38)	I487V	probably benign	Het
Ubr4	C	A	4: 139,408,867 (GRCm38)	C934*	probably null	Het
Vmn1r68	A	G	7: 10,527,632 (GRCm38)	Y180H	probably benign	Het
Vmn2r25	T	C	6: 123,839,923 (GRCm38)	D233G	possibly damaging	Het
Wdr6	A	G	9: 108,576,361 (GRCm38)	W108R	possibly damaging	Het

Other mutations in Cubn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Cubn	APN	2	13,491,820 (GRCm38)	unclassified	probably benign
IGL00228:Cubn	APN	2	13,456,697 (GRCm38)	missense	probably damaging	1.00
IGL00231:Cubn	APN	2	13,381,849 (GRCm38)	missense	possibly damaging	0.89
IGL00327:Cubn	APN	2	13,427,056 (GRCm38)	missense	possibly damaging	0.73
IGL00470:Cubn	APN	2	13,278,418 (GRCm38)	missense	probably benign	0.00
IGL00519:Cubn	APN	2	13,282,919 (GRCm38)	missense	probably benign	0.00
IGL00562:Cubn	APN	2	13,294,230 (GRCm38)	missense	probably benign	0.01
IGL00678:Cubn	APN	2	13,467,710 (GRCm38)	missense	possibly damaging	0.47
IGL00834:Cubn	APN	2	13,381,927 (GRCm38)	missense	probably damaging	1.00
IGL00946:Cubn	APN	2	13,456,623 (GRCm38)	missense	probably damaging	0.98
IGL00971:Cubn	APN	2	13,278,408 (GRCm38)	missense	possibly damaging	0.77
IGL01124:Cubn	APN	2	13,478,093 (GRCm38)	missense	possibly damaging	0.62
IGL01287:Cubn	APN	2	13,310,566 (GRCm38)	missense	probably damaging	1.00
IGL01410:Cubn	APN	2	13,465,908 (GRCm38)	missense	probably benign	0.31
IGL01418:Cubn	APN	2	13,284,041 (GRCm38)	missense	probably benign	0.01
IGL01450:Cubn	APN	2	13,350,862 (GRCm38)	splice site	probably benign
IGL01534:Cubn	APN	2	13,465,933 (GRCm38)	nonsense	probably null
IGL01584:Cubn	APN	2	13,308,661 (GRCm38)	splice site	probably benign
IGL01595:Cubn	APN	2	13,325,216 (GRCm38)	missense	probably benign	0.05
IGL01625:Cubn	APN	2	13,306,274 (GRCm38)	missense	possibly damaging	0.76
IGL01732:Cubn	APN	2	13,489,936 (GRCm38)	nonsense	probably null
IGL01972:Cubn	APN	2	13,446,072 (GRCm38)	missense	possibly damaging	0.90
IGL02027:Cubn	APN	2	13,287,594 (GRCm38)	missense	probably damaging	1.00
IGL02033:Cubn	APN	2	13,339,846 (GRCm38)	missense	probably damaging	0.98
IGL02124:Cubn	APN	2	13,381,837 (GRCm38)	missense	probably damaging	0.99
IGL02335:Cubn	APN	2	13,427,834 (GRCm38)	splice site	probably null
IGL02491:Cubn	APN	2	13,321,228 (GRCm38)	missense	probably damaging	1.00
IGL02686:Cubn	APN	2	13,325,226 (GRCm38)	missense	possibly damaging	0.92
IGL02707:Cubn	APN	2	13,446,032 (GRCm38)	missense	probably damaging	0.99
IGL02746:Cubn	APN	2	13,445,040 (GRCm38)	missense	probably damaging	1.00
IGL02873:Cubn	APN	2	13,294,370 (GRCm38)	missense	probably benign	0.07
IGL02897:Cubn	APN	2	13,318,312 (GRCm38)	missense	possibly damaging	0.55
IGL03078:Cubn	APN	2	13,287,094 (GRCm38)	missense	possibly damaging	0.87
IGL03245:Cubn	APN	2	13,355,689 (GRCm38)	missense	probably benign	0.09
IGL03289:Cubn	APN	2	13,426,967 (GRCm38)	missense	probably benign	0.00
IGL03335:Cubn	APN	2	13,360,329 (GRCm38)	missense	probably damaging	1.00
IGL03355:Cubn	APN	2	13,478,057 (GRCm38)	splice site	probably null
mellow	UTSW	2	13,478,078 (GRCm38)	missense	probably damaging	1.00
PIT4354001:Cubn	UTSW	2	13,468,852 (GRCm38)	nonsense	probably null
PIT4495001:Cubn	UTSW	2	13,491,750 (GRCm38)	missense	probably benign	0.00
R0145:Cubn	UTSW	2	13,306,432 (GRCm38)	missense	probably damaging	1.00
R0220:Cubn	UTSW	2	13,356,709 (GRCm38)	missense	probably damaging	1.00
R0254:Cubn	UTSW	2	13,476,035 (GRCm38)	critical splice donor site	probably null
R0254:Cubn	UTSW	2	13,440,514 (GRCm38)	missense	possibly damaging	0.84
R0254:Cubn	UTSW	2	13,424,694 (GRCm38)	missense	probably benign	0.01
R0360:Cubn	UTSW	2	13,310,507 (GRCm38)	splice site	probably benign
R0364:Cubn	UTSW	2	13,310,507 (GRCm38)	splice site	probably benign
R0383:Cubn	UTSW	2	13,430,959 (GRCm38)	missense	probably damaging	1.00
R0419:Cubn	UTSW	2	13,469,764 (GRCm38)	missense	possibly damaging	0.87
R0419:Cubn	UTSW	2	13,469,763 (GRCm38)	missense	possibly damaging	0.77
R0498:Cubn	UTSW	2	13,444,267 (GRCm38)	missense	probably damaging	0.99
R0560:Cubn	UTSW	2	13,428,680 (GRCm38)	missense	probably damaging	1.00
R0615:Cubn	UTSW	2	13,360,252 (GRCm38)	splice site	probably null
R0735:Cubn	UTSW	2	13,491,689 (GRCm38)	splice site	probably benign
R0780:Cubn	UTSW	2	13,456,613 (GRCm38)	missense	probably damaging	1.00
R0899:Cubn	UTSW	2	13,362,328 (GRCm38)	missense	possibly damaging	0.54
R1118:Cubn	UTSW	2	13,336,242 (GRCm38)	missense	possibly damaging	0.78
R1182:Cubn	UTSW	2	13,445,000 (GRCm38)	missense	probably damaging	0.98
R1439:Cubn	UTSW	2	13,287,568 (GRCm38)	missense	probably damaging	0.96
R1450:Cubn	UTSW	2	13,360,319 (GRCm38)	missense	probably damaging	1.00
R1464:Cubn	UTSW	2	13,325,288 (GRCm38)	missense	possibly damaging	0.87
R1464:Cubn	UTSW	2	13,325,288 (GRCm38)	missense	possibly damaging	0.87
R1476:Cubn	UTSW	2	13,476,120 (GRCm38)	missense	probably benign	0.04
R1508:Cubn	UTSW	2	13,427,105 (GRCm38)	missense	probably benign	0.25
R1532:Cubn	UTSW	2	13,287,661 (GRCm38)	missense	probably damaging	1.00
R1562:Cubn	UTSW	2	13,427,967 (GRCm38)	missense	probably damaging	1.00
R1598:Cubn	UTSW	2	13,469,789 (GRCm38)	missense	probably benign	0.00
R1761:Cubn	UTSW	2	13,489,317 (GRCm38)	critical splice donor site	probably null
R1862:Cubn	UTSW	2	13,308,561 (GRCm38)	missense	probably damaging	1.00
R1874:Cubn	UTSW	2	13,323,002 (GRCm38)	missense	probably damaging	1.00
R1923:Cubn	UTSW	2	13,310,526 (GRCm38)	missense	probably damaging	1.00
R1944:Cubn	UTSW	2	13,278,538 (GRCm38)	missense	probably benign	0.01
R1960:Cubn	UTSW	2	13,340,017 (GRCm38)	splice site	probably null
R2021:Cubn	UTSW	2	13,308,549 (GRCm38)	missense	probably benign	0.09
R2137:Cubn	UTSW	2	13,336,167 (GRCm38)	missense	probably benign	0.01
R2138:Cubn	UTSW	2	13,444,378 (GRCm38)	missense	probably damaging	0.99
R2139:Cubn	UTSW	2	13,336,167 (GRCm38)	missense	probably benign	0.01
R2179:Cubn	UTSW	2	13,318,242 (GRCm38)	missense	possibly damaging	0.85
R2328:Cubn	UTSW	2	13,404,080 (GRCm38)	nonsense	probably null
R2369:Cubn	UTSW	2	13,491,217 (GRCm38)	missense	probably damaging	1.00
R2428:Cubn	UTSW	2	13,476,150 (GRCm38)	missense	probably damaging	1.00
R2435:Cubn	UTSW	2	13,318,272 (GRCm38)	missense	probably damaging	1.00
R2567:Cubn	UTSW	2	13,278,356 (GRCm38)	splice site	probably null
R2850:Cubn	UTSW	2	13,322,953 (GRCm38)	missense	probably damaging	1.00
R2853:Cubn	UTSW	2	13,430,834 (GRCm38)	missense	probably benign	0.00
R2893:Cubn	UTSW	2	13,358,139 (GRCm38)	missense	possibly damaging	0.61
R3107:Cubn	UTSW	2	13,362,347 (GRCm38)	missense	possibly damaging	0.73
R3109:Cubn	UTSW	2	13,362,347 (GRCm38)	missense	possibly damaging	0.73
R3119:Cubn	UTSW	2	13,358,162 (GRCm38)	missense	possibly damaging	0.90
R3405:Cubn	UTSW	2	13,333,508 (GRCm38)	missense	probably benign	0.00
R3703:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3704:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3705:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3764:Cubn	UTSW	2	13,331,585 (GRCm38)	missense	possibly damaging	0.79
R3792:Cubn	UTSW	2	13,427,914 (GRCm38)	missense	probably damaging	1.00
R3802:Cubn	UTSW	2	13,360,353 (GRCm38)	missense	probably benign	0.01
R3813:Cubn	UTSW	2	13,294,325 (GRCm38)	missense	probably damaging	1.00
R3845:Cubn	UTSW	2	13,283,008 (GRCm38)	missense	probably damaging	1.00
R3846:Cubn	UTSW	2	13,283,008 (GRCm38)	missense	probably damaging	1.00
R3900:Cubn	UTSW	2	13,286,980 (GRCm38)	critical splice donor site	probably null
R3921:Cubn	UTSW	2	13,326,677 (GRCm38)	missense	probably damaging	1.00
R4075:Cubn	UTSW	2	13,313,999 (GRCm38)	missense	possibly damaging	0.58
R4082:Cubn	UTSW	2	13,428,563 (GRCm38)	intron	probably benign
R4405:Cubn	UTSW	2	13,466,030 (GRCm38)	missense	probably damaging	1.00
R4615:Cubn	UTSW	2	13,428,749 (GRCm38)	missense	probably damaging	1.00
R4629:Cubn	UTSW	2	13,313,979 (GRCm38)	splice site	probably null
R4770:Cubn	UTSW	2	13,314,767 (GRCm38)	missense	possibly damaging	0.92
R4799:Cubn	UTSW	2	13,351,058 (GRCm38)	missense	probably damaging	1.00
R4799:Cubn	UTSW	2	13,287,024 (GRCm38)	missense	possibly damaging	0.94
R4812:Cubn	UTSW	2	13,459,076 (GRCm38)	missense	probably damaging	1.00
R4825:Cubn	UTSW	2	13,325,225 (GRCm38)	missense	probably damaging	1.00
R4934:Cubn	UTSW	2	13,489,910 (GRCm38)	missense	probably benign	0.06
R4967:Cubn	UTSW	2	13,348,045 (GRCm38)	missense	probably benign	0.01
R5187:Cubn	UTSW	2	13,287,568 (GRCm38)	missense	probably damaging	0.96
R5232:Cubn	UTSW	2	13,478,202 (GRCm38)	nonsense	probably null
R5305:Cubn	UTSW	2	13,388,939 (GRCm38)	missense	probably damaging	1.00
R5506:Cubn	UTSW	2	13,491,695 (GRCm38)	splice site	probably null
R5530:Cubn	UTSW	2	13,308,523 (GRCm38)	missense	probably damaging	1.00
R5531:Cubn	UTSW	2	13,350,932 (GRCm38)	missense	probably benign	0.00
R5737:Cubn	UTSW	2	13,388,891 (GRCm38)	missense	probably damaging	1.00
R5886:Cubn	UTSW	2	13,320,023 (GRCm38)	splice site	probably benign
R5923:Cubn	UTSW	2	13,486,078 (GRCm38)	missense	possibly damaging	0.73
R6032:Cubn	UTSW	2	13,325,184 (GRCm38)	missense	probably benign	0.12
R6032:Cubn	UTSW	2	13,325,184 (GRCm38)	missense	probably benign	0.12
R6084:Cubn	UTSW	2	13,430,897 (GRCm38)	missense	probably damaging	1.00
R6087:Cubn	UTSW	2	13,427,847 (GRCm38)	missense	probably damaging	1.00
R6133:Cubn	UTSW	2	13,308,618 (GRCm38)	missense	probably benign	0.29
R6181:Cubn	UTSW	2	13,349,876 (GRCm38)	missense	probably benign	0.31
R6301:Cubn	UTSW	2	13,478,078 (GRCm38)	missense	probably damaging	1.00
R6320:Cubn	UTSW	2	13,280,195 (GRCm38)	missense	probably damaging	1.00
R6368:Cubn	UTSW	2	13,476,123 (GRCm38)	missense	probably damaging	0.98
R6368:Cubn	UTSW	2	13,430,995 (GRCm38)	missense	probably damaging	0.96
R6383:Cubn	UTSW	2	13,427,835 (GRCm38)	critical splice donor site	probably null
R6393:Cubn	UTSW	2	13,355,680 (GRCm38)	missense	probably benign	0.08
R6408:Cubn	UTSW	2	13,294,203 (GRCm38)	missense	probably damaging	1.00
R6470:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R6532:Cubn	UTSW	2	13,459,002 (GRCm38)	missense	probably benign	0.01
R6599:Cubn	UTSW	2	13,310,673 (GRCm38)	missense	possibly damaging	0.95
R6629:Cubn	UTSW	2	13,430,872 (GRCm38)	missense	probably damaging	1.00
R6641:Cubn	UTSW	2	13,476,064 (GRCm38)	missense	probably damaging	1.00
R6800:Cubn	UTSW	2	13,321,255 (GRCm38)	missense	probably damaging	1.00
R6823:Cubn	UTSW	2	13,445,029 (GRCm38)	missense	probably benign	0.21
R6847:Cubn	UTSW	2	13,444,253 (GRCm38)	critical splice donor site	probably null
R6885:Cubn	UTSW	2	13,318,278 (GRCm38)	missense	probably damaging	1.00
R6962:Cubn	UTSW	2	13,348,029 (GRCm38)	missense	probably benign	0.03
R6973:Cubn	UTSW	2	13,381,837 (GRCm38)	missense	possibly damaging	0.61
R6975:Cubn	UTSW	2	13,486,789 (GRCm38)	missense	probably damaging	0.99
R7076:Cubn	UTSW	2	13,306,281 (GRCm38)	missense	probably benign	0.10
R7076:Cubn	UTSW	2	13,306,280 (GRCm38)	missense	probably benign	0.00
R7086:Cubn	UTSW	2	13,319,858 (GRCm38)	missense	probably damaging	0.98
R7162:Cubn	UTSW	2	13,342,498 (GRCm38)	missense	probably damaging	0.96
R7203:Cubn	UTSW	2	13,351,003 (GRCm38)	missense	probably benign	0.01
R7292:Cubn	UTSW	2	13,424,739 (GRCm38)	missense	probably damaging	0.99
R7307:Cubn	UTSW	2	13,340,332 (GRCm38)	missense	probably damaging	0.99
R7329:Cubn	UTSW	2	13,468,771 (GRCm38)	missense	probably damaging	0.99
R7395:Cubn	UTSW	2	13,287,064 (GRCm38)	missense	probably damaging	1.00
R7417:Cubn	UTSW	2	13,426,967 (GRCm38)	missense	probably benign	0.00
R7429:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R7430:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R7443:Cubn	UTSW	2	13,455,509 (GRCm38)	missense	probably damaging	1.00
R7699:Cubn	UTSW	2	13,348,178 (GRCm38)	missense	probably benign
R7700:Cubn	UTSW	2	13,489,917 (GRCm38)	missense	possibly damaging	0.74
R7700:Cubn	UTSW	2	13,348,178 (GRCm38)	missense	probably benign
R7751:Cubn	UTSW	2	13,360,365 (GRCm38)	missense	probably damaging	1.00
R7755:Cubn	UTSW	2	13,280,078 (GRCm38)	missense	probably benign	0.11
R7759:Cubn	UTSW	2	13,348,150 (GRCm38)	missense	probably damaging	1.00
R7903:Cubn	UTSW	2	13,468,869 (GRCm38)	missense	probably damaging	0.97
R7921:Cubn	UTSW	2	13,424,727 (GRCm38)	missense	probably benign	0.22
R7988:Cubn	UTSW	2	13,332,355 (GRCm38)	missense	probably benign	0.43
R8010:Cubn	UTSW	2	13,336,086 (GRCm38)	critical splice donor site	probably null
R8020:Cubn	UTSW	2	13,479,178 (GRCm38)	missense	probably benign	0.01
R8120:Cubn	UTSW	2	13,331,660 (GRCm38)	missense	probably damaging	1.00
R8133:Cubn	UTSW	2	13,388,848 (GRCm38)	missense	probably damaging	1.00
R8185:Cubn	UTSW	2	13,294,318 (GRCm38)	missense	probably benign	0.11
R8224:Cubn	UTSW	2	13,349,877 (GRCm38)	missense	probably benign	0.16
R8289:Cubn	UTSW	2	13,486,802 (GRCm38)	missense	probably benign	0.10
R8326:Cubn	UTSW	2	13,306,463 (GRCm38)	missense	probably benign	0.01
R8331:Cubn	UTSW	2	13,340,242 (GRCm38)	missense	probably damaging	1.00
R8338:Cubn	UTSW	2	13,430,847 (GRCm38)	missense	probably benign	0.08
R8341:Cubn	UTSW	2	13,428,724 (GRCm38)	missense	probably damaging	1.00
R8358:Cubn	UTSW	2	13,325,160 (GRCm38)	missense	probably benign	0.17
R8427:Cubn	UTSW	2	13,428,756 (GRCm38)	missense	probably benign	0.00
R8432:Cubn	UTSW	2	13,381,799 (GRCm38)	missense	probably benign	0.00
R8441:Cubn	UTSW	2	13,427,847 (GRCm38)	missense	probably damaging	1.00
R8442:Cubn	UTSW	2	13,314,044 (GRCm38)	missense	probably damaging	1.00
R8520:Cubn	UTSW	2	13,308,520 (GRCm38)	critical splice donor site	probably null
R8699:Cubn	UTSW	2	13,383,959 (GRCm38)	missense	probably damaging	1.00
R8753:Cubn	UTSW	2	13,308,566 (GRCm38)	nonsense	probably null
R8874:Cubn	UTSW	2	13,360,346 (GRCm38)	missense	possibly damaging	0.63
R9056:Cubn	UTSW	2	13,456,655 (GRCm38)	missense	probably damaging	1.00
R9079:Cubn	UTSW	2	13,287,103 (GRCm38)	missense	probably benign	0.02
R9143:Cubn	UTSW	2	13,332,465 (GRCm38)	splice site	probably benign
R9261:Cubn	UTSW	2	13,278,451 (GRCm38)	missense	probably damaging	1.00
R9338:Cubn	UTSW	2	13,381,892 (GRCm38)	missense	probably damaging	1.00
R9342:Cubn	UTSW	2	13,458,956 (GRCm38)	missense	probably damaging	0.99
R9603:Cubn	UTSW	2	13,287,699 (GRCm38)	missense	probably damaging	1.00
R9614:Cubn	UTSW	2	13,478,134 (GRCm38)	missense	probably benign	0.00
R9615:Cubn	UTSW	2	13,321,180 (GRCm38)	missense	possibly damaging	0.88
R9616:Cubn	UTSW	2	13,314,718 (GRCm38)	missense	probably benign	0.04
R9774:Cubn	UTSW	2	13,428,719 (GRCm38)	missense	probably benign
X0018:Cubn	UTSW	2	13,458,986 (GRCm38)	missense	probably damaging	1.00
X0022:Cubn	UTSW	2	13,476,076 (GRCm38)	missense	probably damaging	1.00
X0026:Cubn	UTSW	2	13,342,581 (GRCm38)	missense	probably damaging	1.00
X0063:Cubn	UTSW	2	13,322,962 (GRCm38)	missense	probably damaging	1.00
YA93:Cubn	UTSW	2	13,383,992 (GRCm38)	missense	probably benign	0.21
Z1088:Cubn	UTSW	2	13,294,229 (GRCm38)	missense	probably benign	0.43
Z1176:Cubn	UTSW	2	13,381,825 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ATGCTATCAGAACTTGAACAAAGCC -3'
(R):5'- GTCAACTGAGCTTTGCCACATG -3'

Sequencing Primer
(F):5'- CTTGAACAAAGCCTTGCCGTAGTG -3'
(R):5'- AACTGAGCTTTGCCACATGTTTTG -3'

Posted On

2019-11-12