Mutagenetix > Incidental Mutation

Incidental Mutation 'R7699:4932438A13Rik'

593833

Institutional Source

Beutler Lab

Gene Symbol

4932438A13Rik

Ensembl Gene

ENSMUSG00000037270

Gene Name

RIKEN cDNA 4932438A13 gene

Synonyms

Tweek, FSA

MMRRC Submission

045760-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7699 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

36863104-37053033 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 37026154 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Leucine at position 267 (S267L)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057272] [ENSMUST00000138950] [ENSMUST00000152564]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000057272
AA Change: S3687L

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000060199
Gene: ENSMUSG00000037270
AA Change: S3687L

Domain	Start	End	E-Value	Type
transmembrane domain	25	47	N/A	INTRINSIC
low complexity region	701	716	N/A	INTRINSIC
low complexity region	767	779	N/A	INTRINSIC
low complexity region	1127	1138	N/A	INTRINSIC
low complexity region	1154	1166	N/A	INTRINSIC
low complexity region	1226	1240	N/A	INTRINSIC
low complexity region	1381	1402	N/A	INTRINSIC
low complexity region	1541	1547	N/A	INTRINSIC
low complexity region	1593	1607	N/A	INTRINSIC
low complexity region	1810	1821	N/A	INTRINSIC
low complexity region	1981	1995	N/A	INTRINSIC
low complexity region	2182	2191	N/A	INTRINSIC
low complexity region	2336	2349	N/A	INTRINSIC
low complexity region	2614	2657	N/A	INTRINSIC
low complexity region	3468	3480	N/A	INTRINSIC
low complexity region	3717	3742	N/A	INTRINSIC
low complexity region	3816	3837	N/A	INTRINSIC
low complexity region	3919	3929	N/A	INTRINSIC
low complexity region	3941	3948	N/A	INTRINSIC
low complexity region	4024	4038	N/A	INTRINSIC
low complexity region	4041	4049	N/A	INTRINSIC
low complexity region	4117	4149	N/A	INTRINSIC
low complexity region	4172	4185	N/A	INTRINSIC
low complexity region	4359	4380	N/A	INTRINSIC
FSA_C	4386	4990	N/A	SMART
low complexity region	4993	5004	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000138152

SMART Domains

Protein: ENSMUSP00000117809
Gene: ENSMUSG00000037270

Domain	Start	End	E-Value	Type
low complexity region	81	106	N/A	INTRINSIC
low complexity region	180	201	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000138950
AA Change: S224L

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000118092
Gene: ENSMUSG00000037270
AA Change: S224L

Domain	Start	End	E-Value	Type
low complexity region	254	279	N/A	INTRINSIC
low complexity region	353	374	N/A	INTRINSIC
low complexity region	526	540	N/A	INTRINSIC
low complexity region	543	551	N/A	INTRINSIC
low complexity region	619	651	N/A	INTRINSIC
low complexity region	674	687	N/A	INTRINSIC
low complexity region	861	882	N/A	INTRINSIC
FSA_C	888	1492	N/A	SMART
low complexity region	1495	1506	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000141740
AA Change: S267L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000117814
Gene: ENSMUSG00000037270
AA Change: S267L

Domain	Start	End	E-Value	Type
low complexity region	28	40	N/A	INTRINSIC
low complexity region	298	323	N/A	INTRINSIC
low complexity region	397	418	N/A	INTRINSIC
low complexity region	500	510	N/A	INTRINSIC
low complexity region	522	529	N/A	INTRINSIC
low complexity region	605	619	N/A	INTRINSIC
low complexity region	622	630	N/A	INTRINSIC
low complexity region	698	730	N/A	INTRINSIC
low complexity region	753	766	N/A	INTRINSIC
low complexity region	940	961	N/A	INTRINSIC
FSA_C	967	1571	N/A	SMART
low complexity region	1574	1585	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000146948
AA Change: S427L

PolyPhen 2 Score 0.682 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000121356
Gene: ENSMUSG00000037270
AA Change: S427L

Domain	Start	End	E-Value	Type
low complexity region	121	133	N/A	INTRINSIC
low complexity region	167	177	N/A	INTRINSIC
low complexity region	458	483	N/A	INTRINSIC
low complexity region	557	578	N/A	INTRINSIC
low complexity region	730	744	N/A	INTRINSIC
low complexity region	747	755	N/A	INTRINSIC
low complexity region	823	855	N/A	INTRINSIC
low complexity region	878	891	N/A	INTRINSIC
low complexity region	1065	1086	N/A	INTRINSIC
FSA_C	1092	1696	N/A	SMART
low complexity region	1699	1710	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000152564
AA Change: S3687L

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000117808
Gene: ENSMUSG00000037270
AA Change: S3687L

Domain	Start	End	E-Value	Type
transmembrane domain	25	47	N/A	INTRINSIC
low complexity region	701	716	N/A	INTRINSIC
low complexity region	767	779	N/A	INTRINSIC
low complexity region	1127	1138	N/A	INTRINSIC
low complexity region	1154	1166	N/A	INTRINSIC
low complexity region	1226	1240	N/A	INTRINSIC
low complexity region	1381	1402	N/A	INTRINSIC
low complexity region	1541	1547	N/A	INTRINSIC
low complexity region	1593	1607	N/A	INTRINSIC
low complexity region	1810	1821	N/A	INTRINSIC
low complexity region	1981	1995	N/A	INTRINSIC
low complexity region	2182	2191	N/A	INTRINSIC
low complexity region	2336	2349	N/A	INTRINSIC
low complexity region	2614	2657	N/A	INTRINSIC
low complexity region	3468	3480	N/A	INTRINSIC
low complexity region	3717	3742	N/A	INTRINSIC
low complexity region	3816	3837	N/A	INTRINSIC
low complexity region	3919	3929	N/A	INTRINSIC
low complexity region	3941	3948	N/A	INTRINSIC
low complexity region	4024	4038	N/A	INTRINSIC
low complexity region	4041	4049	N/A	INTRINSIC
low complexity region	4117	4149	N/A	INTRINSIC
low complexity region	4172	4185	N/A	INTRINSIC
low complexity region	4359	4380	N/A	INTRINSIC
FSA_C	4386	4990	N/A	SMART
low complexity region	4993	5004	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is located on the long arm of chromosome 4 in a region that is associated with susceptibility to celiac disease. The encoded protein is similar to a Chinese hamster protein that is associated with spermatocyte and adipocyte differentiation. The C-terminus of the protein is also similar to a Caenorhabditis elegans protein that plays a role in lipid storage. In mammals, this protein is thought to function in the regulation of epithelial growth and differentiation, and in tumor development. [provided by RefSeq, Oct 2009]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810009A15Rik	T	C	19: 8,890,053 (GRCm38)	L72P	probably benign	Het
4930407I10Rik	C	A	15: 82,064,105 (GRCm38)	H734Q	probably benign	Het
Acadl	A	G	1: 66,838,363 (GRCm38)	V343A	possibly damaging	Het
Acot4	A	T	12: 84,043,237 (GRCm38)	D236V	probably damaging	Het
App	T	A	16: 85,040,309 (GRCm38)		probably null	Het
Arfgef1	G	A	1: 10,194,411 (GRCm38)	T470I	possibly damaging	Het
Arhgef5	T	C	6: 43,274,757 (GRCm38)	I814T	probably benign	Het
Atr	A	T	9: 95,875,690 (GRCm38)	R968*	probably null	Het
C130060K24Rik	T	A	6: 65,452,956 (GRCm38)	I212N	probably benign	Het
Capn7	C	T	14: 31,352,444 (GRCm38)	T268I	probably benign	Het
Cbwd1	A	G	19: 24,942,681 (GRCm38)		probably null	Het
Cubn	A	G	2: 13,489,917 (GRCm38)	V107A	possibly damaging	Het
Cubn	A	T	2: 13,348,178 (GRCm38)	F1916L	probably benign	Het
Cyp11b1	T	A	15: 74,835,842 (GRCm38)	T473S	probably damaging	Het
Cyr61	C	T	3: 145,648,692 (GRCm38)	G155R	probably damaging	Het
Dip2c	T	G	13: 9,659,311 (GRCm38)	S1396A	probably benign	Het
Dyrk1b	T	C	7: 28,184,312 (GRCm38)	Y198H	probably damaging	Het
Echdc2	G	T	4: 108,174,077 (GRCm38)	R206L	probably benign	Het
Ep400	C	T	5: 110,696,032 (GRCm38)	R1594Q	unknown	Het
Fmnl2	G	A	2: 53,036,508 (GRCm38)	R69Q		Het
Fry	T	A	5: 150,405,327 (GRCm38)	H1308Q	probably damaging	Het
Fyb2	T	A	4: 105,010,454 (GRCm38)	D667E	probably benign	Het
Gimap7	T	A	6: 48,723,857 (GRCm38)	Y126N	possibly damaging	Het
Gm1979	A	T	5: 26,000,180 (GRCm38)	W266R	probably damaging	Het
Gm28042	T	A	2: 120,039,716 (GRCm38)	M712K	possibly damaging	Het
Gm3238	A	G	10: 77,770,635 (GRCm38)	*231R	probably null	Het
Gm5592	C	A	7: 41,286,407 (GRCm38)	A111E	probably damaging	Het
Gm7694	A	G	1: 170,301,148 (GRCm38)	*271Q	probably null	Het
Ift81	T	C	5: 122,594,560 (GRCm38)	M304V	possibly damaging	Het
Igfals	T	C	17: 24,880,574 (GRCm38)	L213P	probably damaging	Het
Ints3	G	A	3: 90,421,804 (GRCm38)	P83S	probably benign	Het
Kcna10	T	G	3: 107,195,540 (GRCm38)	S496A	probably damaging	Het
Kmt2d	G	A	15: 98,843,719 (GRCm38)	A4520V	unknown	Het
Lemd3	A	T	10: 120,978,090 (GRCm38)	Y413N	probably damaging	Het
Lgr6	T	C	1: 134,996,032 (GRCm38)	N453S	probably damaging	Het
Lmtk3	A	C	7: 45,792,574 (GRCm38)	D352A	probably damaging	Het
Lrrc4c	T	C	2: 97,630,679 (GRCm38)	M550T	possibly damaging	Het
Maml2	A	T	9: 13,621,089 (GRCm38)	Q533L		Het
Mdn1	T	A	4: 32,741,344 (GRCm38)	D3815E	probably damaging	Het
Megf8	C	T	7: 25,329,928 (GRCm38)	A299V	possibly damaging	Het
Meis3	G	T	7: 16,177,556 (GRCm38)	E59D	probably benign	Het
Mppe1	A	G	18: 67,225,704 (GRCm38)	*398R	probably null	Het
Mtmr7	G	A	8: 40,606,884 (GRCm38)	A62V	possibly damaging	Het
Mtus1	T	A	8: 41,083,969 (GRCm38)	T237S	possibly damaging	Het
Ndfip2	T	A	14: 105,287,759 (GRCm38)	V158E	possibly damaging	Het
Nrbp2	T	G	15: 76,090,897 (GRCm38)	T53P	probably damaging	Het
Nrde2	G	A	12: 100,130,835 (GRCm38)	P902L	probably benign	Het
Olfr1383	A	G	11: 49,524,554 (GRCm38)	Y277C	probably damaging	Het
Olfr509	T	A	7: 108,645,672 (GRCm38)	K301N	probably damaging	Het
Olfr642	T	A	7: 104,050,593 (GRCm38)		probably benign	Het
Olfr739	T	A	14: 50,425,335 (GRCm38)	M272K	probably benign	Het
Olfr771	T	C	10: 129,160,055 (GRCm38)	M310V	probably benign	Het
Pcdha1	A	G	18: 36,931,062 (GRCm38)	T260A	probably damaging	Het
Pdlim1	T	G	19: 40,249,658 (GRCm38)	K98N	probably damaging	Het
Phrf1	G	A	7: 141,254,929 (GRCm38)	G207R	unknown	Het
Pik3r6	A	G	11: 68,528,563 (GRCm38)	K124R	probably damaging	Het
Polr2b	T	A	5: 77,340,421 (GRCm38)	F823I	probably benign	Het
Prss58	T	C	6: 40,895,388 (GRCm38)	I234V	probably damaging	Het
Ptpn14	A	T	1: 189,865,411 (GRCm38)	N766I	probably benign	Het
Repin1	T	A	6: 48,597,822 (GRCm38)	S562T	probably damaging	Het
Rgr	C	T	14: 37,044,595 (GRCm38)	D165N	probably damaging	Het
Rho	T	C	6: 115,935,239 (GRCm38)	F221L	probably damaging	Het
Rictor	C	T	15: 6,772,154 (GRCm38)	S441L	probably benign	Het
Rnase2a	A	G	14: 51,255,791 (GRCm38)	I39T	probably damaging	Het
Rpl13a	T	G	7: 45,127,236 (GRCm38)	I43L	probably benign	Het
Rpp30	T	C	19: 36,089,158 (GRCm38)	V97A	probably benign	Het
Scube3	T	C	17: 28,167,049 (GRCm38)	Y755H	probably damaging	Het
Sec24a	A	T	11: 51,712,257 (GRCm38)	V788E	probably damaging	Het
Spns2	A	T	11: 72,489,617 (GRCm38)	L60*	probably null	Het
Strc	T	C	2: 121,371,748 (GRCm38)	N1213S	possibly damaging	Het
Tec	A	T	5: 72,786,024 (GRCm38)	I116N	possibly damaging	Het
Tll1	A	T	8: 64,093,954 (GRCm38)	D319E	probably benign	Het
Tmem121b	T	C	6: 120,493,427 (GRCm38)	T110A	unknown	Het
Tmem232	T	A	17: 65,265,218 (GRCm38)	I593F	probably damaging	Het
Tpp2	A	G	1: 43,970,466 (GRCm38)	I487V	probably benign	Het
Ubr4	C	A	4: 139,408,867 (GRCm38)	C934*	probably null	Het
Vmn1r68	A	G	7: 10,527,632 (GRCm38)	Y180H	probably benign	Het
Vmn2r25	T	C	6: 123,839,923 (GRCm38)	D233G	possibly damaging	Het
Wdr6	A	G	9: 108,576,361 (GRCm38)	W108R	possibly damaging	Het

Other mutations in 4932438A13Rik

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:4932438A13Rik	APN	3	37,011,727 (GRCm38)	missense	probably benign	0.00
IGL00434:4932438A13Rik	APN	3	36,987,299 (GRCm38)	missense	probably damaging	0.98
IGL00640:4932438A13Rik	APN	3	36,908,218 (GRCm38)	missense	probably damaging	1.00
IGL00693:4932438A13Rik	APN	3	37,052,547 (GRCm38)	utr 3 prime	probably benign
IGL00721:4932438A13Rik	APN	3	37,030,751 (GRCm38)	splice site	probably null
IGL00756:4932438A13Rik	APN	3	36,908,218 (GRCm38)	missense	probably damaging	1.00
IGL00896:4932438A13Rik	APN	3	37,039,462 (GRCm38)	missense	probably benign
IGL00902:4932438A13Rik	APN	3	37,041,345 (GRCm38)	missense	probably damaging	1.00
IGL00980:4932438A13Rik	APN	3	37,000,041 (GRCm38)	missense	probably damaging	1.00
IGL01019:4932438A13Rik	APN	3	37,006,984 (GRCm38)	critical splice acceptor site	probably null
IGL01025:4932438A13Rik	APN	3	37,046,280 (GRCm38)	missense	possibly damaging	0.89
IGL01306:4932438A13Rik	APN	3	37,005,013 (GRCm38)	splice site	probably benign
IGL01370:4932438A13Rik	APN	3	36,947,755 (GRCm38)	missense	probably benign	0.07
IGL01377:4932438A13Rik	APN	3	36,973,452 (GRCm38)	critical splice donor site	probably null
IGL01401:4932438A13Rik	APN	3	36,942,292 (GRCm38)	missense	probably benign
IGL01419:4932438A13Rik	APN	3	37,048,121 (GRCm38)	missense	probably damaging	1.00
IGL01432:4932438A13Rik	APN	3	37,003,759 (GRCm38)	missense	possibly damaging	0.87
IGL01433:4932438A13Rik	APN	3	36,887,770 (GRCm38)	missense	probably damaging	1.00
IGL01452:4932438A13Rik	APN	3	36,996,308 (GRCm38)	unclassified	probably benign
IGL01520:4932438A13Rik	APN	3	36,973,260 (GRCm38)	nonsense	probably null
IGL01524:4932438A13Rik	APN	3	36,942,382 (GRCm38)	missense	possibly damaging	0.90
IGL01628:4932438A13Rik	APN	3	37,008,485 (GRCm38)	missense	probably damaging	1.00
IGL01638:4932438A13Rik	APN	3	36,974,311 (GRCm38)	missense	probably damaging	1.00
IGL01650:4932438A13Rik	APN	3	36,992,673 (GRCm38)	splice site	probably benign
IGL01717:4932438A13Rik	APN	3	37,034,736 (GRCm38)	missense	probably benign
IGL01767:4932438A13Rik	APN	3	37,041,363 (GRCm38)	missense	probably benign	0.29
IGL01813:4932438A13Rik	APN	3	36,928,520 (GRCm38)	missense	possibly damaging	0.90
IGL01998:4932438A13Rik	APN	3	36,957,016 (GRCm38)	missense	possibly damaging	0.49
IGL02172:4932438A13Rik	APN	3	37,004,873 (GRCm38)	missense	probably damaging	0.99
IGL02197:4932438A13Rik	APN	3	36,906,735 (GRCm38)	missense	probably damaging	1.00
IGL02248:4932438A13Rik	APN	3	36,969,290 (GRCm38)	critical splice donor site	probably null
IGL02273:4932438A13Rik	APN	3	36,921,437 (GRCm38)	splice site	probably benign
IGL02403:4932438A13Rik	APN	3	37,030,664 (GRCm38)	missense	probably benign
IGL02492:4932438A13Rik	APN	3	37,048,113 (GRCm38)	missense	probably benign	0.04
IGL02517:4932438A13Rik	APN	3	36,958,868 (GRCm38)	missense	probably damaging	1.00
IGL02519:4932438A13Rik	APN	3	36,895,315 (GRCm38)	missense	probably damaging	1.00
IGL02586:4932438A13Rik	APN	3	37,044,608 (GRCm38)	nonsense	probably null
IGL02620:4932438A13Rik	APN	3	37,035,945 (GRCm38)	missense	possibly damaging	0.95
IGL02621:4932438A13Rik	APN	3	37,041,484 (GRCm38)	splice site	probably benign
IGL02670:4932438A13Rik	APN	3	36,967,305 (GRCm38)	nonsense	probably null
IGL02806:4932438A13Rik	APN	3	36,946,494 (GRCm38)	missense	possibly damaging	0.95
IGL02985:4932438A13Rik	APN	3	36,958,757 (GRCm38)	missense	probably damaging	0.99
IGL03004:4932438A13Rik	APN	3	36,965,677 (GRCm38)	splice site	probably benign
IGL03037:4932438A13Rik	APN	3	36,969,207 (GRCm38)	missense	probably benign	0.23
IGL03037:4932438A13Rik	APN	3	36,969,208 (GRCm38)	missense	probably damaging	1.00
IGL03062:4932438A13Rik	APN	3	37,038,517 (GRCm38)	splice site	probably benign
IGL03137:4932438A13Rik	APN	3	37,034,602 (GRCm38)	missense	probably damaging	0.98
IGL03150:4932438A13Rik	APN	3	36,948,066 (GRCm38)	missense	probably damaging	1.00
IGL03204:4932438A13Rik	APN	3	37,050,934 (GRCm38)	splice site	probably benign
IGL03207:4932438A13Rik	APN	3	36,949,996 (GRCm38)	missense	possibly damaging	0.73
IGL03256:4932438A13Rik	APN	3	36,906,683 (GRCm38)	splice site	probably benign
IGL03264:4932438A13Rik	APN	3	37,002,635 (GRCm38)	missense	probably damaging	1.00
IGL03265:4932438A13Rik	APN	3	37,047,991 (GRCm38)	missense	probably benign	0.00
IGL03303:4932438A13Rik	APN	3	36,870,077 (GRCm38)	missense	possibly damaging	0.90
admonished	UTSW	3	36,948,304 (GRCm38)	missense	probably damaging	1.00
alerted	UTSW	3	37,033,265 (GRCm38)	missense	possibly damaging	0.85
informed	UTSW	3	36,965,849 (GRCm38)	missense	probably damaging	1.00
resolved	UTSW	3	36,921,221 (GRCm38)	missense	possibly damaging	0.60
tipped	UTSW	3	36,988,085 (GRCm38)	missense	possibly damaging	0.81
warned	UTSW	3	36,965,621 (GRCm38)	missense	probably damaging	1.00
FR4340:4932438A13Rik	UTSW	3	37,050,752 (GRCm38)	critical splice acceptor site	probably benign
FR4737:4932438A13Rik	UTSW	3	37,050,754 (GRCm38)	critical splice acceptor site	probably benign
PIT4515001:4932438A13Rik	UTSW	3	36,974,236 (GRCm38)	missense	probably damaging	1.00
R0035:4932438A13Rik	UTSW	3	36,987,598 (GRCm38)	nonsense	probably null
R0047:4932438A13Rik	UTSW	3	36,908,192 (GRCm38)	missense	possibly damaging	0.83
R0047:4932438A13Rik	UTSW	3	36,908,192 (GRCm38)	missense	possibly damaging	0.83
R0068:4932438A13Rik	UTSW	3	36,952,221 (GRCm38)	missense	probably benign	0.28
R0068:4932438A13Rik	UTSW	3	36,952,221 (GRCm38)	missense	probably benign	0.28
R0092:4932438A13Rik	UTSW	3	37,028,159 (GRCm38)	missense	probably benign	0.41
R0233:4932438A13Rik	UTSW	3	36,948,563 (GRCm38)	nonsense	probably null
R0233:4932438A13Rik	UTSW	3	36,948,563 (GRCm38)	nonsense	probably null
R0256:4932438A13Rik	UTSW	3	36,917,773 (GRCm38)	missense	probably benign	0.01
R0277:4932438A13Rik	UTSW	3	36,943,182 (GRCm38)	nonsense	probably null
R0321:4932438A13Rik	UTSW	3	36,906,788 (GRCm38)	splice site	probably null
R0323:4932438A13Rik	UTSW	3	36,943,182 (GRCm38)	nonsense	probably null
R0335:4932438A13Rik	UTSW	3	36,969,152 (GRCm38)	missense	probably damaging	1.00
R0375:4932438A13Rik	UTSW	3	37,046,252 (GRCm38)	missense	probably damaging	0.99
R0437:4932438A13Rik	UTSW	3	36,989,804 (GRCm38)	missense	possibly damaging	0.81
R0445:4932438A13Rik	UTSW	3	37,000,065 (GRCm38)	missense	probably damaging	0.99
R0496:4932438A13Rik	UTSW	3	36,987,635 (GRCm38)	missense	probably damaging	1.00
R0531:4932438A13Rik	UTSW	3	37,036,825 (GRCm38)	missense	probably damaging	1.00
R0543:4932438A13Rik	UTSW	3	36,996,458 (GRCm38)	missense	probably benign	0.22
R0545:4932438A13Rik	UTSW	3	36,987,690 (GRCm38)	splice site	probably benign
R0674:4932438A13Rik	UTSW	3	37,044,626 (GRCm38)	missense	possibly damaging	0.86
R0745:4932438A13Rik	UTSW	3	36,928,463 (GRCm38)	missense	probably damaging	1.00
R0755:4932438A13Rik	UTSW	3	36,946,364 (GRCm38)	missense	probably damaging	1.00
R0785:4932438A13Rik	UTSW	3	36,959,334 (GRCm38)	splice site	probably benign
R1056:4932438A13Rik	UTSW	3	37,044,680 (GRCm38)	missense	probably benign	0.44
R1056:4932438A13Rik	UTSW	3	36,983,453 (GRCm38)	missense	possibly damaging	0.69
R1080:4932438A13Rik	UTSW	3	36,988,255 (GRCm38)	missense	probably damaging	1.00
R1103:4932438A13Rik	UTSW	3	36,996,523 (GRCm38)	missense	probably benign
R1119:4932438A13Rik	UTSW	3	36,987,045 (GRCm38)	missense	probably damaging	1.00
R1170:4932438A13Rik	UTSW	3	37,044,631 (GRCm38)	missense	probably damaging	0.98
R1183:4932438A13Rik	UTSW	3	36,895,303 (GRCm38)	missense	possibly damaging	0.51
R1186:4932438A13Rik	UTSW	3	36,996,312 (GRCm38)	unclassified	probably benign
R1201:4932438A13Rik	UTSW	3	36,948,375 (GRCm38)	missense	probably benign
R1219:4932438A13Rik	UTSW	3	36,946,470 (GRCm38)	nonsense	probably null
R1270:4932438A13Rik	UTSW	3	36,952,184 (GRCm38)	missense	probably damaging	1.00
R1273:4932438A13Rik	UTSW	3	36,987,210 (GRCm38)	missense	probably damaging	1.00
R1338:4932438A13Rik	UTSW	3	37,052,535 (GRCm38)	missense	unknown
R1364:4932438A13Rik	UTSW	3	36,987,030 (GRCm38)	missense	probably damaging	1.00
R1437:4932438A13Rik	UTSW	3	36,942,429 (GRCm38)	missense	possibly damaging	0.65
R1447:4932438A13Rik	UTSW	3	36,965,586 (GRCm38)	missense	probably damaging	0.98
R1467:4932438A13Rik	UTSW	3	37,035,945 (GRCm38)	missense	probably damaging	0.99
R1467:4932438A13Rik	UTSW	3	37,035,945 (GRCm38)	missense	probably damaging	0.99
R1470:4932438A13Rik	UTSW	3	36,998,331 (GRCm38)	missense	probably benign	0.31
R1470:4932438A13Rik	UTSW	3	36,998,331 (GRCm38)	missense	probably benign	0.31
R1481:4932438A13Rik	UTSW	3	37,008,434 (GRCm38)	missense	probably damaging	0.99
R1528:4932438A13Rik	UTSW	3	37,052,535 (GRCm38)	missense	unknown
R1533:4932438A13Rik	UTSW	3	37,041,375 (GRCm38)	missense	probably damaging	1.00
R1546:4932438A13Rik	UTSW	3	36,870,056 (GRCm38)	missense	possibly damaging	0.64
R1606:4932438A13Rik	UTSW	3	36,942,399 (GRCm38)	missense	probably damaging	1.00
R1638:4932438A13Rik	UTSW	3	37,035,812 (GRCm38)	nonsense	probably null
R1772:4932438A13Rik	UTSW	3	36,959,432 (GRCm38)	missense	probably damaging	1.00
R1896:4932438A13Rik	UTSW	3	36,908,231 (GRCm38)	nonsense	probably null
R1919:4932438A13Rik	UTSW	3	37,006,983 (GRCm38)	critical splice acceptor site	probably null
R1983:4932438A13Rik	UTSW	3	36,887,865 (GRCm38)	missense	probably null	1.00
R1987:4932438A13Rik	UTSW	3	36,953,985 (GRCm38)	critical splice donor site	probably null
R1992:4932438A13Rik	UTSW	3	37,000,032 (GRCm38)	missense	probably benign	0.32
R1999:4932438A13Rik	UTSW	3	36,908,211 (GRCm38)	missense	probably damaging	1.00
R2004:4932438A13Rik	UTSW	3	36,895,378 (GRCm38)	missense	possibly damaging	0.77
R2010:4932438A13Rik	UTSW	3	36,928,551 (GRCm38)	missense	probably benign	0.09
R2027:4932438A13Rik	UTSW	3	37,047,961 (GRCm38)	splice site	probably benign
R2039:4932438A13Rik	UTSW	3	37,003,878 (GRCm38)	missense	possibly damaging	0.66
R2054:4932438A13Rik	UTSW	3	36,947,853 (GRCm38)	missense	probably benign	0.01
R2089:4932438A13Rik	UTSW	3	36,988,256 (GRCm38)	missense	probably damaging	1.00
R2091:4932438A13Rik	UTSW	3	36,988,256 (GRCm38)	missense	probably damaging	1.00
R2091:4932438A13Rik	UTSW	3	36,988,256 (GRCm38)	missense	probably damaging	1.00
R2091:4932438A13Rik	UTSW	3	36,953,970 (GRCm38)	missense	probably damaging	1.00
R2220:4932438A13Rik	UTSW	3	36,875,530 (GRCm38)	critical splice donor site	probably null
R2374:4932438A13Rik	UTSW	3	36,885,396 (GRCm38)	missense	probably benign	0.00
R2437:4932438A13Rik	UTSW	3	36,958,685 (GRCm38)	splice site	probably null
R2860:4932438A13Rik	UTSW	3	36,965,849 (GRCm38)	missense	probably damaging	1.00
R2861:4932438A13Rik	UTSW	3	36,965,849 (GRCm38)	missense	probably damaging	1.00
R2909:4932438A13Rik	UTSW	3	36,947,953 (GRCm38)	missense	probably damaging	1.00
R2925:4932438A13Rik	UTSW	3	37,007,122 (GRCm38)	missense	probably damaging	0.99
R2940:4932438A13Rik	UTSW	3	36,958,805 (GRCm38)	missense	probably damaging	1.00
R3015:4932438A13Rik	UTSW	3	36,875,462 (GRCm38)	missense	probably damaging	1.00
R3086:4932438A13Rik	UTSW	3	37,011,703 (GRCm38)	missense	possibly damaging	0.56
R3159:4932438A13Rik	UTSW	3	36,959,415 (GRCm38)	missense	probably benign	0.17
R3440:4932438A13Rik	UTSW	3	37,041,912 (GRCm38)	nonsense	probably null
R3703:4932438A13Rik	UTSW	3	36,987,581 (GRCm38)	missense	probably damaging	1.00
R3705:4932438A13Rik	UTSW	3	36,987,581 (GRCm38)	missense	probably damaging	1.00
R3795:4932438A13Rik	UTSW	3	37,030,565 (GRCm38)	missense	probably benign	0.30
R3820:4932438A13Rik	UTSW	3	37,040,434 (GRCm38)	missense	probably damaging	1.00
R3862:4932438A13Rik	UTSW	3	36,885,398 (GRCm38)	missense	possibly damaging	0.73
R3944:4932438A13Rik	UTSW	3	37,030,061 (GRCm38)	missense	possibly damaging	0.90
R4020:4932438A13Rik	UTSW	3	37,012,575 (GRCm38)	intron	probably benign
R4091:4932438A13Rik	UTSW	3	37,030,589 (GRCm38)	missense	probably benign	0.00
R4159:4932438A13Rik	UTSW	3	36,931,083 (GRCm38)	missense	probably benign	0.00
R4231:4932438A13Rik	UTSW	3	36,920,236 (GRCm38)	missense	probably benign	0.10
R4368:4932438A13Rik	UTSW	3	36,988,147 (GRCm38)	nonsense	probably null
R4413:4932438A13Rik	UTSW	3	36,958,681 (GRCm38)	splice site	probably null
R4475:4932438A13Rik	UTSW	3	37,040,395 (GRCm38)	missense	probably damaging	1.00
R4488:4932438A13Rik	UTSW	3	37,003,933 (GRCm38)	missense	probably null	0.93
R4489:4932438A13Rik	UTSW	3	37,003,933 (GRCm38)	missense	probably null	0.93
R4516:4932438A13Rik	UTSW	3	36,895,311 (GRCm38)	missense	possibly damaging	0.90
R4580:4932438A13Rik	UTSW	3	37,030,025 (GRCm38)	missense	probably benign	0.02
R4672:4932438A13Rik	UTSW	3	36,889,990 (GRCm38)	makesense	probably null
R4705:4932438A13Rik	UTSW	3	37,041,889 (GRCm38)	missense	probably benign	0.03
R4735:4932438A13Rik	UTSW	3	37,004,967 (GRCm38)	missense	possibly damaging	0.84
R4741:4932438A13Rik	UTSW	3	36,942,375 (GRCm38)	missense	probably damaging	0.99
R4754:4932438A13Rik	UTSW	3	37,022,466 (GRCm38)	nonsense	probably null
R4778:4932438A13Rik	UTSW	3	36,937,065 (GRCm38)	missense	possibly damaging	0.90
R4833:4932438A13Rik	UTSW	3	36,964,968 (GRCm38)	missense	probably damaging	0.96
R4896:4932438A13Rik	UTSW	3	36,965,937 (GRCm38)	missense	probably damaging	1.00
R4910:4932438A13Rik	UTSW	3	36,998,199 (GRCm38)	missense	probably damaging	1.00
R4922:4932438A13Rik	UTSW	3	36,987,165 (GRCm38)	missense	probably damaging	1.00
R4941:4932438A13Rik	UTSW	3	36,919,901 (GRCm38)	missense	probably benign	0.41
R4941:4932438A13Rik	UTSW	3	36,917,702 (GRCm38)	missense	probably damaging	1.00
R4980:4932438A13Rik	UTSW	3	36,943,312 (GRCm38)	missense	probably damaging	1.00
R5030:4932438A13Rik	UTSW	3	36,943,399 (GRCm38)	intron	probably benign
R5049:4932438A13Rik	UTSW	3	37,041,390 (GRCm38)	missense	probably damaging	1.00
R5049:4932438A13Rik	UTSW	3	37,040,506 (GRCm38)	intron	probably benign
R5089:4932438A13Rik	UTSW	3	36,987,502 (GRCm38)	missense	probably benign	0.02
R5092:4932438A13Rik	UTSW	3	37,000,085 (GRCm38)	missense	probably benign	0.14
R5122:4932438A13Rik	UTSW	3	37,034,757 (GRCm38)	splice site	probably null
R5210:4932438A13Rik	UTSW	3	37,033,265 (GRCm38)	missense	possibly damaging	0.85
R5246:4932438A13Rik	UTSW	3	37,048,050 (GRCm38)	missense	probably damaging	1.00
R5289:4932438A13Rik	UTSW	3	37,000,109 (GRCm38)	missense	probably damaging	0.97
R5348:4932438A13Rik	UTSW	3	37,048,146 (GRCm38)	missense	probably damaging	1.00
R5394:4932438A13Rik	UTSW	3	36,917,668 (GRCm38)	missense	probably damaging	1.00
R5434:4932438A13Rik	UTSW	3	36,875,516 (GRCm38)	missense	probably damaging	1.00
R5667:4932438A13Rik	UTSW	3	36,917,677 (GRCm38)	missense	probably benign	0.00
R5686:4932438A13Rik	UTSW	3	36,917,660 (GRCm38)	missense	probably benign	0.00
R5701:4932438A13Rik	UTSW	3	36,921,360 (GRCm38)	missense	probably benign	0.10
R5778:4932438A13Rik	UTSW	3	36,958,714 (GRCm38)	missense	probably damaging	1.00
R5787:4932438A13Rik	UTSW	3	36,992,733 (GRCm38)	splice site	probably null
R5800:4932438A13Rik	UTSW	3	37,052,443 (GRCm38)	missense	probably damaging	1.00
R5819:4932438A13Rik	UTSW	3	37,048,600 (GRCm38)	missense	probably benign	0.12
R5820:4932438A13Rik	UTSW	3	37,039,526 (GRCm38)	missense	probably benign	0.00
R5952:4932438A13Rik	UTSW	3	36,965,621 (GRCm38)	missense	probably damaging	1.00
R5975:4932438A13Rik	UTSW	3	36,969,221 (GRCm38)	missense	possibly damaging	0.64
R5996:4932438A13Rik	UTSW	3	36,931,116 (GRCm38)	missense	probably benign	0.07
R6192:4932438A13Rik	UTSW	3	36,988,169 (GRCm38)	missense	probably benign	0.00
R6225:4932438A13Rik	UTSW	3	36,948,304 (GRCm38)	missense	probably damaging	1.00
R6234:4932438A13Rik	UTSW	3	36,983,471 (GRCm38)	missense	probably benign	0.00
R6244:4932438A13Rik	UTSW	3	36,956,999 (GRCm38)	missense	probably benign
R6263:4932438A13Rik	UTSW	3	36,931,111 (GRCm38)	missense	probably benign	0.06
R6351:4932438A13Rik	UTSW	3	36,908,228 (GRCm38)	missense	probably damaging	1.00
R6380:4932438A13Rik	UTSW	3	37,033,307 (GRCm38)	missense	probably benign	0.19
R6468:4932438A13Rik	UTSW	3	37,008,443 (GRCm38)	missense	probably damaging	1.00
R6759:4932438A13Rik	UTSW	3	36,988,085 (GRCm38)	missense	possibly damaging	0.81
R6792:4932438A13Rik	UTSW	3	37,011,566 (GRCm38)	critical splice acceptor site	probably null
R6809:4932438A13Rik	UTSW	3	36,874,282 (GRCm38)	missense	probably damaging	0.98
R6841:4932438A13Rik	UTSW	3	37,021,481 (GRCm38)	missense	probably damaging	1.00
R6959:4932438A13Rik	UTSW	3	36,967,189 (GRCm38)	missense	probably damaging	1.00
R7102:4932438A13Rik	UTSW	3	36,940,798 (GRCm38)	missense	probably damaging	0.99
R7188:4932438A13Rik	UTSW	3	36,950,013 (GRCm38)	missense	probably benign	0.06
R7212:4932438A13Rik	UTSW	3	37,048,009 (GRCm38)	missense
R7425:4932438A13Rik	UTSW	3	36,983,394 (GRCm38)	missense	probably benign	0.02
R7425:4932438A13Rik	UTSW	3	36,948,341 (GRCm38)	missense	probably benign
R7451:4932438A13Rik	UTSW	3	37,022,807 (GRCm38)	splice site	probably null
R7604:4932438A13Rik	UTSW	3	36,949,843 (GRCm38)	splice site	probably null
R7622:4932438A13Rik	UTSW	3	36,948,413 (GRCm38)	nonsense	probably null
R7671:4932438A13Rik	UTSW	3	36,943,231 (GRCm38)	missense	probably damaging	0.99
R7699:4932438A13Rik	UTSW	3	36,974,172 (GRCm38)	missense	possibly damaging	0.67
R7700:4932438A13Rik	UTSW	3	36,974,172 (GRCm38)	missense	possibly damaging	0.67
R7700:4932438A13Rik	UTSW	3	37,026,154 (GRCm38)	missense	probably benign	0.00
R7748:4932438A13Rik	UTSW	3	36,959,335 (GRCm38)	critical splice acceptor site	probably null
R7767:4932438A13Rik	UTSW	3	36,920,287 (GRCm38)	critical splice donor site	probably null
R7787:4932438A13Rik	UTSW	3	36,885,408 (GRCm38)	missense	probably damaging	1.00
R7830:4932438A13Rik	UTSW	3	36,964,932 (GRCm38)	frame shift	probably null
R7849:4932438A13Rik	UTSW	3	37,026,328 (GRCm38)	missense
R7912:4932438A13Rik	UTSW	3	37,007,069 (GRCm38)	missense	probably damaging	0.99
R7914:4932438A13Rik	UTSW	3	36,946,283 (GRCm38)	missense	probably benign	0.13
R7945:4932438A13Rik	UTSW	3	36,965,893 (GRCm38)	missense	probably benign	0.03
R8039:4932438A13Rik	UTSW	3	36,943,214 (GRCm38)	missense	probably benign	0.12
R8101:4932438A13Rik	UTSW	3	37,008,502 (GRCm38)	missense	probably damaging	1.00
R8143:4932438A13Rik	UTSW	3	36,946,508 (GRCm38)	critical splice donor site	probably null
R8145:4932438A13Rik	UTSW	3	36,998,267 (GRCm38)	missense	probably damaging	1.00
R8171:4932438A13Rik	UTSW	3	36,975,713 (GRCm38)	missense	probably benign	0.00
R8210:4932438A13Rik	UTSW	3	37,012,881 (GRCm38)	missense
R8250:4932438A13Rik	UTSW	3	36,917,662 (GRCm38)	missense	probably damaging	0.99
R8369:4932438A13Rik	UTSW	3	37,011,603 (GRCm38)	missense
R8478:4932438A13Rik	UTSW	3	37,033,277 (GRCm38)	missense	possibly damaging	0.74
R8558:4932438A13Rik	UTSW	3	37,048,601 (GRCm38)	missense
R8688:4932438A13Rik	UTSW	3	37,035,917 (GRCm38)	missense
R8724:4932438A13Rik	UTSW	3	36,890,893 (GRCm38)	missense	probably damaging	0.99
R8818:4932438A13Rik	UTSW	3	36,996,548 (GRCm38)	missense	possibly damaging	0.60
R8869:4932438A13Rik	UTSW	3	36,958,858 (GRCm38)	missense	probably damaging	0.99
R8887:4932438A13Rik	UTSW	3	37,033,354 (GRCm38)	missense	possibly damaging	0.95
R8899:4932438A13Rik	UTSW	3	36,988,280 (GRCm38)	missense	probably damaging	1.00
R8907:4932438A13Rik	UTSW	3	36,948,146 (GRCm38)	nonsense	probably null
R8960:4932438A13Rik	UTSW	3	37,012,983 (GRCm38)	missense	probably damaging	1.00
R8990:4932438A13Rik	UTSW	3	36,921,221 (GRCm38)	missense	possibly damaging	0.60
R9021:4932438A13Rik	UTSW	3	36,998,344 (GRCm38)	missense	probably benign	0.00
R9048:4932438A13Rik	UTSW	3	37,011,777 (GRCm38)	missense
R9100:4932438A13Rik	UTSW	3	37,044,758 (GRCm38)	missense
R9166:4932438A13Rik	UTSW	3	36,987,367 (GRCm38)	missense	probably damaging	1.00
R9176:4932438A13Rik	UTSW	3	36,956,703 (GRCm38)	missense	possibly damaging	0.82
R9202:4932438A13Rik	UTSW	3	36,890,821 (GRCm38)	missense	probably benign
R9303:4932438A13Rik	UTSW	3	37,044,820 (GRCm38)	missense
R9305:4932438A13Rik	UTSW	3	37,044,820 (GRCm38)	missense
R9332:4932438A13Rik	UTSW	3	37,050,840 (GRCm38)	missense
R9362:4932438A13Rik	UTSW	3	36,957,013 (GRCm38)	missense	probably benign
R9493:4932438A13Rik	UTSW	3	37,011,736 (GRCm38)	missense
R9534:4932438A13Rik	UTSW	3	36,998,270 (GRCm38)	missense	probably benign	0.01
R9569:4932438A13Rik	UTSW	3	37,012,621 (GRCm38)	missense
R9593:4932438A13Rik	UTSW	3	36,947,941 (GRCm38)	missense	probably damaging	1.00
R9600:4932438A13Rik	UTSW	3	37,041,416 (GRCm38)	nonsense	probably null
R9733:4932438A13Rik	UTSW	3	37,048,583 (GRCm38)	missense
R9751:4932438A13Rik	UTSW	3	37,011,740 (GRCm38)	missense
RF013:4932438A13Rik	UTSW	3	37,050,757 (GRCm38)	critical splice acceptor site	probably benign
RF015:4932438A13Rik	UTSW	3	37,050,748 (GRCm38)	critical splice acceptor site	probably benign
RF021:4932438A13Rik	UTSW	3	37,050,748 (GRCm38)	critical splice acceptor site	probably benign
RF023:4932438A13Rik	UTSW	3	37,050,760 (GRCm38)	critical splice acceptor site	probably benign
RF034:4932438A13Rik	UTSW	3	37,050,760 (GRCm38)	critical splice acceptor site	probably benign
RF035:4932438A13Rik	UTSW	3	37,050,758 (GRCm38)	critical splice acceptor site	probably benign
RF055:4932438A13Rik	UTSW	3	37,050,757 (GRCm38)	critical splice acceptor site	probably benign
X0050:4932438A13Rik	UTSW	3	36,957,128 (GRCm38)	missense	probably damaging	1.00
Z1088:4932438A13Rik	UTSW	3	36,987,567 (GRCm38)	missense	probably damaging	1.00
Z1177:4932438A13Rik	UTSW	3	36,983,440 (GRCm38)	missense	possibly damaging	0.88
Z1177:4932438A13Rik	UTSW	3	36,919,950 (GRCm38)	missense	probably benign
Z1177:4932438A13Rik	UTSW	3	37,036,707 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- AGTGAGGAAATCAACTCAGAGCTTC -3'
(R):5'- AGTAAATGGCTACTCACGAGGG -3'

Sequencing Primer
(F):5'- GAAATCAACTCAGAGCTTCTAAAATG -3'
(R):5'- AAATGGCTACTCACGAGGGTTTCC -3'

Posted On

2019-11-12