Mutagenetix > Incidental Mutation

Incidental Mutation 'R7701:Miip'

593993

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

045762-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R7701 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

147945235-147953176 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 147947371 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 237 (V237E)

Ref Sequence

ENSEMBL: ENSMUSP00000113897 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably null
Transcript: ENSMUST00000030886
AA Change: V237E

PolyPhen 2 Score 0.793 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: V237E

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000119975
AA Change: V237E

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: V237E

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000172710
AA Change: V237E

PolyPhen 2 Score 0.793 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: V237E

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Meta Mutation Damage Score

0.1624

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930470P17Rik	T	C	2: 170,443,225 (GRCm39)		probably benign	Het
4932414N04Rik	G	A	2: 68,561,548 (GRCm39)	V292M	possibly damaging	Het
Actn1	A	T	12: 80,221,328 (GRCm39)	V575E	possibly damaging	Het
Arhgef1	T	C	7: 24,612,003 (GRCm39)	S129P	probably benign	Het
Aup1	T	C	6: 83,032,908 (GRCm39)	V214A	probably benign	Het
Bbs10	A	G	10: 111,135,874 (GRCm39)	E329G	probably damaging	Het
Brinp2	A	G	1: 158,094,030 (GRCm39)		probably null	Het
Ccdc3	A	G	2: 5,142,868 (GRCm39)	T42A	possibly damaging	Het
Cd276	T	C	9: 58,442,810 (GRCm39)	N215S	probably benign	Het
Col24a1	T	A	3: 145,020,772 (GRCm39)	M381K	probably benign	Het
Col24a1	A	G	3: 145,072,656 (GRCm39)		probably null	Het
Col6a4	A	C	9: 105,960,087 (GRCm39)	F19V	probably benign	Het
Crybg1	G	T	10: 43,865,139 (GRCm39)	A1446E	probably benign	Het
Dach1	C	T	14: 98,140,670 (GRCm39)	R496K	probably damaging	Het
Dchs2	A	G	3: 83,253,513 (GRCm39)	T2308A	possibly damaging	Het
Duxf1	A	G	10: 58,058,885 (GRCm39)	V623A	possibly damaging	Het
Dync1h1	G	A	12: 110,585,080 (GRCm39)	D828N	probably damaging	Het
Eif2a	A	T	3: 58,459,991 (GRCm39)	H462L	possibly damaging	Het
Flot2	A	G	11: 77,928,942 (GRCm39)		probably null	Het
Gas2	T	G	7: 51,643,101 (GRCm39)	Y263*	probably null	Het
Gm5111	A	G	6: 48,567,027 (GRCm39)	I81V	unknown	Het
Iqcm	A	G	8: 76,281,539 (GRCm39)	I7M	probably benign	Het
Kank1	G	A	19: 25,389,129 (GRCm39)		probably null	Het
Lnx2	A	T	5: 146,961,333 (GRCm39)	V533E	probably damaging	Het
Mapk8ip3	G	T	17: 25,120,378 (GRCm39)	P904T	possibly damaging	Het
Mcm5	C	A	8: 75,850,551 (GRCm39)	H596N	probably benign	Het
Mob3a	G	A	10: 80,525,768 (GRCm39)	A181V	probably damaging	Het
Mrpl38	G	A	11: 116,026,104 (GRCm39)	R99W	probably benign	Het
Naa25	A	G	5: 121,564,042 (GRCm39)	T486A	probably benign	Het
Or2z9	T	A	8: 72,854,030 (GRCm39)	L142Q	probably damaging	Het
Or5b102	A	G	19: 13,041,445 (GRCm39)	I223M	probably damaging	Het
Or8g4	A	G	9: 39,662,597 (GRCm39)	Y305C	probably benign	Het
Pcdha8	A	T	18: 37,126,864 (GRCm39)	N449Y	probably damaging	Het
Pcdhb6	A	T	18: 37,467,562 (GRCm39)	D161V	probably damaging	Het
Pdlim3	A	G	8: 46,361,576 (GRCm39)	D134G	probably benign	Het
Phpt1	A	G	2: 25,464,799 (GRCm39)	V18A	probably benign	Het
Prg4	T	C	1: 150,333,293 (GRCm39)	K177E	possibly damaging	Het
Psmb1	A	G	17: 15,697,509 (GRCm39)	F202S	probably benign	Het
Rab14	A	G	2: 35,073,427 (GRCm39)	F150L		Het
Rgs14	A	G	13: 55,527,138 (GRCm39)	D169G	probably damaging	Het
Rreb1	C	T	13: 38,114,092 (GRCm39)	L484F	possibly damaging	Het
Rsph14	A	T	10: 74,793,608 (GRCm39)	Y264*	probably null	Het
Scly	T	C	1: 91,236,030 (GRCm39)	I152T		Het
Ska1	A	T	18: 74,335,714 (GRCm39)	H85Q	probably damaging	Het
Slc35b3	T	C	13: 39,128,611 (GRCm39)	M159V	probably benign	Het
Smok2b	A	G	17: 13,453,767 (GRCm39)		probably benign	Het
Spock1	G	A	13: 57,735,472 (GRCm39)	Q103*	probably null	Het
Topbp1	T	C	9: 103,210,184 (GRCm39)	V914A	probably damaging	Het
Tspan3	A	T	9: 56,054,803 (GRCm39)	Y41*	probably null	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,376,118 (GRCm39)		probably null	Het
Ttn	T	G	2: 76,560,028 (GRCm39)	I29458L	possibly damaging	Het
Tubgcp3	A	G	8: 12,705,974 (GRCm39)	S183P	probably benign	Het
Zfat	C	A	15: 68,052,757 (GRCm39)	E346*	probably null	Het
Zfp341	T	A	2: 154,476,000 (GRCm39)		probably null	Het
Zfp54	A	G	17: 21,654,357 (GRCm39)	T284A	probably benign	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147,950,322 (GRCm39)	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147,949,735 (GRCm39)	splice site	probably benign
IGL02829:Miip	APN	4	147,947,518 (GRCm39)	missense	probably benign	0.01
IGL03350:Miip	APN	4	147,946,979 (GRCm39)	missense	probably benign	0.01
R0200:Miip	UTSW	4	147,946,720 (GRCm39)	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147,949,691 (GRCm39)	missense	probably benign	0.02
R1783:Miip	UTSW	4	147,950,231 (GRCm39)	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147,947,549 (GRCm39)	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147,950,422 (GRCm39)	missense	probably benign	0.15
R3615:Miip	UTSW	4	147,950,371 (GRCm39)	missense	probably benign	0.00
R3616:Miip	UTSW	4	147,950,371 (GRCm39)	missense	probably benign	0.00
R3882:Miip	UTSW	4	147,945,509 (GRCm39)	missense	possibly damaging	0.93
R4579:Miip	UTSW	4	147,945,518 (GRCm39)	missense	probably damaging	1.00
R5183:Miip	UTSW	4	147,947,526 (GRCm39)	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147,950,135 (GRCm39)	missense	probably benign	0.00
R6056:Miip	UTSW	4	147,946,792 (GRCm39)	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147,947,540 (GRCm39)	missense	probably benign	0.12
R6568:Miip	UTSW	4	147,950,372 (GRCm39)	missense	probably benign
R6603:Miip	UTSW	4	147,950,380 (GRCm39)	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147,947,021 (GRCm39)	missense	probably benign	0.22
R7795:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7796:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7797:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7872:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7920:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R8468:Miip	UTSW	4	147,945,928 (GRCm39)	missense	probably damaging	1.00
R8492:Miip	UTSW	4	147,945,881 (GRCm39)	missense	probably damaging	1.00
R8677:Miip	UTSW	4	147,947,503 (GRCm39)	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147,950,839 (GRCm39)	start gained	probably benign
R8860:Miip	UTSW	4	147,950,839 (GRCm39)	start gained	probably benign
R9755:Miip	UTSW	4	147,950,319 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAGGTCAGGGAGAAATGTCCTC -3'
(R):5'- AGCTCTCCAGTCACTAGTGAG -3'

Sequencing Primer
(F):5'- AGAAATGTCCTCCCAGGGG -3'
(R):5'- AGTCACTAGTGAGCCCGAG -3'

Posted On

2019-11-12