Mutagenetix > Incidental Mutation

Incidental Mutation 'R7707:Taar1'

594354

Institutional Source

Beutler Lab

Gene Symbol

Taar1

Ensembl Gene

ENSMUSG00000056379

Gene Name

trace amine-associated receptor 1

Synonyms

Trar1, Tar1

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.094) question?

Stock #

R7707 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23920356-23921469 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23921237 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 278 (I278F)

Ref Sequence

ENSEMBL: ENSMUSP00000049527 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051532]

AlphaFold

Q923Y8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000051532
AA Change: I278F

PolyPhen 2 Score 0.691 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000049527
Gene: ENSMUSG00000056379
AA Change: I278F

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	316	1.4e-11	PFAM
Pfam:7tm_1	39	301	2.4e-62	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (72/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a G-protein coupled receptor activated by trace amines. The encoded protein responds little or not at all to dopamine, serotonin, epinephrine, or histamine, but responds well to beta-phenylethylamine, p-tyramine, octopamine, and tryptamine. While primarily functioning in neurologic systems, there is evidence that this gene is involved in blood cell and immunologic functions as well. This gene is thought to be intronless. [provided by RefSeq, Nov 2015]
PHENOTYPE: Mice homozygous for a null mutation display decreased prepulse inhibition and increased sensitivity to amphetamines. Mice homozygous for another knock-out allele exhibit increased sensitivity to MDMA-induced hyperthermia, brain dopamine and serotonin levels, and induced hyperactivity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610044O15Rik8	A	T	8: 129,219,179	C388*	probably null	Het
2900026A02Rik	T	A	5: 113,137,986	M1L	probably benign	Het
Ap1s3	T	C	1: 79,614,247	K129E	probably benign	Het
Ap3b2	C	T	7: 81,476,782	V357I	possibly damaging	Het
Aplp1	A	T	7: 30,443,098	C140S	probably damaging	Het
Arhgef1	G	A	7: 24,916,881	D317N	probably damaging	Het
Asb4	A	G	6: 5,430,968	H401R	probably benign	Het
Bpi	A	T	2: 158,261,173	E79D	probably benign	Het
Cant1	C	T	11: 118,410,898	V198M	possibly damaging	Het
Casp9	C	T	4: 141,805,467	R225C	probably benign	Het
Ccdc88b	C	A	19: 6,857,469	R82L	probably benign	Het
Chrm4	A	G	2: 91,927,354	T36A	probably benign	Het
Cntln	A	T	4: 84,884,616	D51V	probably damaging	Het
Commd8	A	T	5: 72,162,738	F120Y	probably damaging	Het
Cpne6	A	C	14: 55,516,314	T410P	probably damaging	Het
Ctnnb1	A	T	9: 120,952,865	I315F	possibly damaging	Het
Dnah9	A	G	11: 66,118,958	V701A	probably damaging	Het
Efcab9	A	G	11: 32,522,851	Y199H	possibly damaging	Het
Endou	T	A	15: 97,713,102		probably null	Het
Fam160a1	A	G	3: 85,676,253	V412A	probably benign	Het
Foxc2	C	T	8: 121,117,902	P430S	probably benign	Het
Gas2l3	T	A	10: 89,414,358	K299N	probably damaging	Het
Gm10375	G	A	14: 43,604,875	Q133*	probably null	Het
Gorab	T	C	1: 163,392,440	D211G	probably damaging	Het
Grin3b	T	A	10: 79,975,901	S747T	possibly damaging	Het
Gucd1	C	A	10: 75,511,286		probably benign	Het
Gucy2d	T	C	7: 98,451,669	F400L	possibly damaging	Het
Hivep3	G	A	4: 119,733,959	V55M		Het
Igsf3	A	G	3: 101,459,922	N1157S	probably benign	Het
Irak3	A	T	10: 120,146,584	D324E	probably damaging	Het
Jup	G	T	11: 100,383,052	A221D	possibly damaging	Het
Kctd17	CAGCTGGAGGAGC	CAGC	15: 78,436,913		probably benign	Het
Lgr4	A	G	2: 109,997,591		probably null	Het
Lrrc34	T	C	3: 30,624,892	D352G	probably benign	Het
Metrn	C	A	17: 25,795,410	A175S	probably benign	Het
Nr2c1	T	A	10: 94,188,165	S411T	probably benign	Het
Olfr1206	A	G	2: 88,864,809	D68G	possibly damaging	Het
Olfr190	T	A	16: 59,074,271	I270F	possibly damaging	Het
Orc3	T	A	4: 34,598,691	K172*	probably null	Het
Oxnad1	A	G	14: 32,102,008		probably null	Het
Pcdh7	A	G	5: 57,720,330	N409S	probably damaging	Het
Pcdha11	A	T	18: 37,011,792	N312I	probably benign	Het
Pds5a	G	T	5: 65,610,133	P121Q	unknown	Het
Phc1	A	G	6: 122,323,780	I380T	unknown	Het
Phldb3	C	A	7: 24,626,597	H535N	possibly damaging	Het
Proser3	T	C	7: 30,539,791	Q600R	probably benign	Het
Ptprz1	A	G	6: 23,002,296	M1462V	probably benign	Het
Pyroxd2	T	C	19: 42,738,147	T243A	probably damaging	Het
Ralgapa1	C	A	12: 55,777,292	D268Y	probably null	Het
Rapgef5	A	C	12: 117,715,344	Y419S	probably damaging	Het
Rbm24	C	A	13: 46,429,129	Q175K	possibly damaging	Het
Robo4	A	T	9: 37,413,122	D982V	probably damaging	Het
Sbf2	T	C	7: 110,330,713		probably null	Het
Serping1	A	T	2: 84,773,699		probably null	Het
Shank1	C	T	7: 44,344,301	S798F	unknown	Het
Slc15a5	A	T	6: 138,079,747	M57K	probably damaging	Het
Slc35g1	T	A	19: 38,403,123	C284*	probably null	Het
Src	G	A	2: 157,464,658	D194N	probably damaging	Het
Srfbp1	A	G	18: 52,483,654	T84A	probably damaging	Het
Sspo	A	T	6: 48,461,527	T1510S	probably benign	Het
Taf1a	T	C	1: 183,404,429	Y281H	possibly damaging	Het
Thbs3	A	T	3: 89,224,900	Y798F	possibly damaging	Het
Tnpo1	T	C	13: 98,890,787	T7A	probably benign	Het
Traf7	A	T	17: 24,510,709		probably null	Het
Trbv19	G	A	6: 41,178,613	V9I	possibly damaging	Het
Trim17	C	T	11: 58,965,284	Q56*	probably null	Het
Ttn	G	T	2: 76,902,062	A4643E	unknown	Het
Ugt2b36	A	G	5: 87,081,508		probably null	Het
Uso1	A	G	5: 92,201,936	*960W	probably null	Het
Usp2	G	T	9: 44,073,460		probably null	Het
Wdr20rt	A	G	12: 65,226,207	D148G	probably damaging	Het
Wdr66	A	T	5: 123,253,887	E28V	probably benign	Het
Wif1	T	C	10: 121,083,959	F204L	probably damaging	Het
Wwp1	T	C	4: 19,627,645	D750G	probably benign	Het
Zmynd12	A	T	4: 119,444,866	D234V	probably damaging	Het

Other mutations in Taar1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00782:Taar1	APN	10	23920446	missense	probably benign	0.00
R0022:Taar1	UTSW	10	23920727	missense	probably benign	0.45
R0126:Taar1	UTSW	10	23920547	missense	probably benign	0.00
R0442:Taar1	UTSW	10	23920482	missense	possibly damaging	0.80
R0720:Taar1	UTSW	10	23921073	missense	probably damaging	1.00
R0931:Taar1	UTSW	10	23921283	missense	probably damaging	1.00
R1270:Taar1	UTSW	10	23920533	missense	probably damaging	1.00
R1834:Taar1	UTSW	10	23921189	missense	probably benign	0.04
R2137:Taar1	UTSW	10	23921270	missense	probably benign
R3765:Taar1	UTSW	10	23921307	missense	probably damaging	1.00
R3873:Taar1	UTSW	10	23920584	missense	probably damaging	1.00
R5334:Taar1	UTSW	10	23920545	missense	probably damaging	1.00
R5418:Taar1	UTSW	10	23921316	missense	possibly damaging	0.91
R5578:Taar1	UTSW	10	23920820	missense	possibly damaging	0.71
R7048:Taar1	UTSW	10	23920824	missense	probably benign	0.40
R7096:Taar1	UTSW	10	23920911	missense	possibly damaging	0.48
R7163:Taar1	UTSW	10	23921020	missense	probably benign	0.38
R7854:Taar1	UTSW	10	23920782	missense	probably benign	0.00
R8036:Taar1	UTSW	10	23921135	missense	probably benign	0.01
R8442:Taar1	UTSW	10	23920624	nonsense	probably null
R8855:Taar1	UTSW	10	23921078	missense	possibly damaging	0.50

Predicted Primers

PCR Primer
(F):5'- CAAGGTCAATCAATCGTACGAATG -3'
(R):5'- TGTGCCTATAGTTGGCTCAC -3'

Sequencing Primer
(F):5'- CAATCGTACGAATGTTCAAGTTGG -3'
(R):5'- GAACAGTCAGTGCTCTTAGTCACTG -3'

Posted On

2019-11-12