Incidental Mutation 'R7707:Efcab9'
ID594360
Institutional Source Beutler Lab
Gene Symbol Efcab9
Ensembl Gene ENSMUSG00000044056
Gene NameEF-hand calcium binding domain 9
Synonyms
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.058) question?
Stock #R7707 (G1)
Quality Score225.009
Status Validated
Chromosome11
Chromosomal Location32522751-32532081 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 32522851 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Histidine at position 199 (Y199H)
Ref Sequence ENSEMBL: ENSMUSP00000060205 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000054327] [ENSMUST00000109377]
Predicted Effect possibly damaging
Transcript: ENSMUST00000054327
AA Change: Y199H

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000060205
Gene: ENSMUSG00000044056
AA Change: Y199H

DomainStartEndE-ValueType
SCOP:d2sas__ 46 182 7e-9 SMART
Blast:EFh 63 91 1e-7 BLAST
Blast:EFh 140 170 5e-9 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000109377
SMART Domains Protein: ENSMUSP00000105002
Gene: ENSMUSG00000044056

DomainStartEndE-ValueType
Blast:EFh 1 26 3e-7 BLAST
SCOP:d2sas__ 2 88 2e-4 SMART
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 99% (72/73)
Allele List at MGI
Other mutations in this stock
Total: 75 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2610044O15Rik8 A T 8: 129,219,179 C388* probably null Het
2900026A02Rik T A 5: 113,137,986 M1L probably benign Het
Ap1s3 T C 1: 79,614,247 K129E probably benign Het
Ap3b2 C T 7: 81,476,782 V357I possibly damaging Het
Aplp1 A T 7: 30,443,098 C140S probably damaging Het
Arhgef1 G A 7: 24,916,881 D317N probably damaging Het
Asb4 A G 6: 5,430,968 H401R probably benign Het
Bpi A T 2: 158,261,173 E79D probably benign Het
Cant1 C T 11: 118,410,898 V198M possibly damaging Het
Casp9 C T 4: 141,805,467 R225C probably benign Het
Ccdc88b C A 19: 6,857,469 R82L probably benign Het
Chrm4 A G 2: 91,927,354 T36A probably benign Het
Cntln A T 4: 84,884,616 D51V probably damaging Het
Commd8 A T 5: 72,162,738 F120Y probably damaging Het
Cpne6 A C 14: 55,516,314 T410P probably damaging Het
Ctnnb1 A T 9: 120,952,865 I315F possibly damaging Het
Dnah9 A G 11: 66,118,958 V701A probably damaging Het
Endou T A 15: 97,713,102 probably null Het
Fam160a1 A G 3: 85,676,253 V412A probably benign Het
Foxc2 C T 8: 121,117,902 P430S probably benign Het
Gas2l3 T A 10: 89,414,358 K299N probably damaging Het
Gm10375 G A 14: 43,604,875 Q133* probably null Het
Gorab T C 1: 163,392,440 D211G probably damaging Het
Grin3b T A 10: 79,975,901 S747T possibly damaging Het
Gucd1 C A 10: 75,511,286 probably benign Het
Gucy2d T C 7: 98,451,669 F400L possibly damaging Het
Hivep3 G A 4: 119,733,959 V55M Het
Igsf3 A G 3: 101,459,922 N1157S probably benign Het
Irak3 A T 10: 120,146,584 D324E probably damaging Het
Jup G T 11: 100,383,052 A221D possibly damaging Het
Kctd17 CAGCTGGAGGAGC CAGC 15: 78,436,913 probably benign Het
Lgr4 A G 2: 109,997,591 probably null Het
Lrrc34 T C 3: 30,624,892 D352G probably benign Het
Metrn C A 17: 25,795,410 A175S probably benign Het
Nr2c1 T A 10: 94,188,165 S411T probably benign Het
Olfr1206 A G 2: 88,864,809 D68G possibly damaging Het
Olfr190 T A 16: 59,074,271 I270F possibly damaging Het
Orc3 T A 4: 34,598,691 K172* probably null Het
Oxnad1 A G 14: 32,102,008 probably null Het
Pcdh7 A G 5: 57,720,330 N409S probably damaging Het
Pcdha11 A T 18: 37,011,792 N312I probably benign Het
Pds5a G T 5: 65,610,133 P121Q unknown Het
Phc1 A G 6: 122,323,780 I380T unknown Het
Phldb3 C A 7: 24,626,597 H535N possibly damaging Het
Proser3 T C 7: 30,539,791 Q600R probably benign Het
Ptprz1 A G 6: 23,002,296 M1462V probably benign Het
Pyroxd2 T C 19: 42,738,147 T243A probably damaging Het
Ralgapa1 C A 12: 55,777,292 D268Y probably null Het
Rapgef5 A C 12: 117,715,344 Y419S probably damaging Het
Rbm24 C A 13: 46,429,129 Q175K possibly damaging Het
Robo4 A T 9: 37,413,122 D982V probably damaging Het
Sbf2 T C 7: 110,330,713 probably null Het
Serping1 A T 2: 84,773,699 probably null Het
Shank1 C T 7: 44,344,301 S798F unknown Het
Slc15a5 A T 6: 138,079,747 M57K probably damaging Het
Slc35g1 T A 19: 38,403,123 C284* probably null Het
Src G A 2: 157,464,658 D194N probably damaging Het
Srfbp1 A G 18: 52,483,654 T84A probably damaging Het
Sspo A T 6: 48,461,527 T1510S probably benign Het
Taar1 A T 10: 23,921,237 I278F possibly damaging Het
Taf1a T C 1: 183,404,429 Y281H possibly damaging Het
Thbs3 A T 3: 89,224,900 Y798F possibly damaging Het
Tnpo1 T C 13: 98,890,787 T7A probably benign Het
Traf7 A T 17: 24,510,709 probably null Het
Trbv19 G A 6: 41,178,613 V9I possibly damaging Het
Trim17 C T 11: 58,965,284 Q56* probably null Het
Ttn G T 2: 76,902,062 A4643E unknown Het
Ugt2b36 A G 5: 87,081,508 probably null Het
Uso1 A G 5: 92,201,936 *960W probably null Het
Usp2 G T 9: 44,073,460 probably null Het
Wdr20rt A G 12: 65,226,207 D148G probably damaging Het
Wdr66 A T 5: 123,253,887 E28V probably benign Het
Wif1 T C 10: 121,083,959 F204L probably damaging Het
Wwp1 T C 4: 19,627,645 D750G probably benign Het
Zmynd12 A T 4: 119,444,866 D234V probably damaging Het
Other mutations in Efcab9
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01700:Efcab9 APN 11 32527451 missense probably damaging 1.00
IGL02419:Efcab9 APN 11 32522950 missense probably benign 0.17
PIT4366001:Efcab9 UTSW 11 32523608 missense probably damaging 1.00
R1764:Efcab9 UTSW 11 32524457 missense possibly damaging 0.88
R3081:Efcab9 UTSW 11 32523689 missense probably benign 0.01
R3751:Efcab9 UTSW 11 32527420 missense probably benign 0.05
R5237:Efcab9 UTSW 11 32522832 missense probably benign 0.01
R5375:Efcab9 UTSW 11 32527484 missense probably damaging 1.00
R5475:Efcab9 UTSW 11 32522862 missense probably damaging 0.96
R7754:Efcab9 UTSW 11 32522941 missense possibly damaging 0.56
Predicted Primers PCR Primer
(F):5'- CAGACATAGACACCCACTTTGG -3'
(R):5'- TTGTAAGCACCCAGAGTACCAC -3'

Sequencing Primer
(F):5'- TATGAGTCTGAGACCAGCCTGATC -3'
(R):5'- GTACCACACAATTACCACCAGTTAC -3'
Posted On2019-11-12