Mutagenetix > Incidental Mutation

Incidental Mutation 'R7710:Kras'

594532

Institutional Source

Beutler Lab

Gene Symbol

Kras

Ensembl Gene

ENSMUSG00000030265

Gene Name

Kirsten rat sarcoma viral oncogene homolog

Synonyms

Kras2, Kras-2, K-ras, Ki-ras

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7710 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

145162425-145195965 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 145166354 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 183 (T183A)

Ref Sequence

ENSEMBL: ENSMUSP00000032399 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032399] [ENSMUST00000039729] [ENSMUST00000111710] [ENSMUST00000111719] [ENSMUST00000111721] [ENSMUST00000111723] [ENSMUST00000111724] [ENSMUST00000111725] [ENSMUST00000111726] [ENSMUST00000156486] [ENSMUST00000203147]

AlphaFold

P32883

Predicted Effect

probably benign
Transcript: ENSMUST00000032399
AA Change: T183A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000032399
Gene: ENSMUSG00000030265
AA Change: T183A

Domain	Start	End	E-Value	Type
RAS	1	166	1.14e-123	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000039729

SMART Domains

Protein: ENSMUSP00000039433
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111710

SMART Domains

Protein: ENSMUSP00000107339
Gene: ENSMUSG00000030265

Domain	Start	End	E-Value	Type
RAS	1	166	3.7e-123	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000111719

SMART Domains

Protein: ENSMUSP00000107348
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111721

SMART Domains

Protein: ENSMUSP00000107350
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	5.5e-15	PFAM
Pfam:Complex1_LYR_1	7	67	5.5e-15	PFAM
Pfam:Complex1_LYR_2	9	85	9.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111723

SMART Domains

Protein: ENSMUSP00000107352
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111724

SMART Domains

Protein: ENSMUSP00000107353
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111725

SMART Domains

Protein: ENSMUSP00000107354
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111726

SMART Domains

Protein: ENSMUSP00000107355
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156486

Predicted Effect

unknown
Transcript: ENSMUST00000203147
AA Change: T70A

SMART Domains

Protein: ENSMUSP00000145294
Gene: ENSMUSG00000030265
AA Change: T70A

Domain	Start	End	E-Value	Type
small_GTPase	1	53	3.1e-8	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality, decreased fetal growth, pericardial edema, anemia, and liver hypoplasia. Mice heterozygous for various knock-in alleles exhibit increased tumorigenesis. [provided by MGI curators]

Allele List at MGI

All alleles(26) : Targeted, knock-out(3) Targeted, other(7) Gene trapped(14) Other(2)

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamtsl1	G	A	4: 86,150,810 (GRCm39)	E151K		Het
Ap3b1	T	C	13: 94,587,581 (GRCm39)	S452P	probably damaging	Het
Cacna2d4	A	G	6: 119,251,200 (GRCm39)	I463V	probably benign	Het
Calhm4	A	T	10: 33,920,045 (GRCm39)	M74K	possibly damaging	Het
Ccdc142	T	C	6: 83,078,677 (GRCm39)	S5P	probably benign	Het
Cd300ld	C	T	11: 114,875,038 (GRCm39)	V199M	probably damaging	Het
Cep162	T	C	9: 87,114,172 (GRCm39)	Y300C	probably damaging	Het
Ces2h	C	T	8: 105,727,497 (GRCm39)	Q5*	probably null	Het
Col9a3	C	T	2: 180,251,158 (GRCm39)	L310F	probably damaging	Het
Copa	A	G	1: 171,937,411 (GRCm39)	D454G	possibly damaging	Het
Ctnnbip1	A	C	4: 149,630,277 (GRCm39)	M29L	probably benign	Het
Ctnnbl1	A	G	2: 157,616,491 (GRCm39)	D64G	probably benign	Het
Dcxr	T	A	11: 120,617,908 (GRCm39)	T23S	probably benign	Het
Dennd1b	T	A	1: 138,990,670 (GRCm39)	H211Q	probably damaging	Het
Ercc8	G	A	13: 108,320,397 (GRCm39)	A328T	probably benign	Het
Fam217a	T	C	13: 35,095,111 (GRCm39)	D216G	possibly damaging	Het
Fat2	T	A	11: 55,201,589 (GRCm39)	Y495F	probably benign	Het
Fbxw13	G	A	9: 109,024,968 (GRCm39)	S15F	probably damaging	Het
Fras1	T	A	5: 96,792,962 (GRCm39)	C964*	probably null	Het
Fshr	T	A	17: 89,292,683 (GRCm39)	H665L	probably benign	Het
Gm10447	A	T	11: 53,347,437 (GRCm39)	C31S	unknown	Het
Gm13272	A	T	4: 88,698,586 (GRCm39)	Q167L	probably benign	Het
Gtpbp2	T	C	17: 46,478,713 (GRCm39)	I510T	possibly damaging	Het
Hps6	G	A	19: 45,993,007 (GRCm39)	A315T	probably benign	Het
Igfbp6	A	C	15: 102,056,285 (GRCm39)	T115P	probably damaging	Het
Ikzf4	A	G	10: 128,468,610 (GRCm39)	V623A	possibly damaging	Het
Il11ra1	T	C	4: 41,764,846 (GRCm39)	V100A	probably benign	Het
Ints1	T	C	5: 139,756,840 (GRCm39)	T493A	probably benign	Het
Itgax	C	T	7: 127,735,028 (GRCm39)	T453I	probably benign	Het
Lhcgr	T	C	17: 89,050,210 (GRCm39)	T439A	probably damaging	Het
Lrrn4	T	C	2: 132,721,451 (GRCm39)	H122R	probably benign	Het
Muc2	A	T	7: 141,287,452 (GRCm39)	R210S	possibly damaging	Het
Myh7	C	G	14: 55,226,258 (GRCm39)	D461H	probably damaging	Het
Myo18b	T	C	5: 113,022,891 (GRCm39)	D167G	unknown	Het
Nedd8	G	A	14: 55,909,446 (GRCm39)		probably benign	Het
Or5b94	A	C	19: 12,652,340 (GRCm39)	Y257S	probably damaging	Het
Pars2	T	A	4: 106,511,276 (GRCm39)	Y353N	probably damaging	Het
Pbxip1	T	A	3: 89,355,408 (GRCm39)	D642E	probably damaging	Het
Pcdhb10	A	T	18: 37,546,654 (GRCm39)	R577*	probably null	Het
Pcna-ps2	T	A	19: 9,261,489 (GRCm39)	Y249*	probably null	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Pla2g6	A	T	15: 79,171,358 (GRCm39)	V744E	probably damaging	Het
Ppfibp1	A	G	6: 146,897,903 (GRCm39)	I207V	probably benign	Het
Prr11	T	C	11: 86,994,433 (GRCm39)	D71G	probably benign	Het
Rnf150	A	C	8: 83,590,781 (GRCm39)	Y48S	probably damaging	Het
Rsf1	G	A	7: 97,331,041 (GRCm39)	G1237D		Het
Rusc2	C	T	4: 43,416,119 (GRCm39)	T475I	probably benign	Het
Scgb1b20	T	A	7: 33,072,867 (GRCm39)	I25N	probably damaging	Het
Sdr39u1	T	C	14: 56,137,116 (GRCm39)	N65S	probably benign	Het
Sh3gl3	T	C	7: 81,933,294 (GRCm39)	V219A	possibly damaging	Het
Shcbp1	A	G	8: 4,814,965 (GRCm39)	F171S	probably benign	Het
Shq1	A	G	6: 100,648,006 (GRCm39)	F6S	probably damaging	Het
Slc12a4	C	T	8: 106,672,203 (GRCm39)	R868H	possibly damaging	Het
Smg6	T	C	11: 74,821,445 (GRCm39)	V572A	probably benign	Het
Syn3	A	G	10: 86,243,534 (GRCm39)	V135A	probably damaging	Het
Tars3	A	T	7: 65,314,717 (GRCm39)	D425V	probably benign	Het
Tmem253	A	T	14: 52,254,608 (GRCm39)	Q21L	possibly damaging	Het
Trpm3	G	T	19: 22,896,154 (GRCm39)	R997L	probably damaging	Het
Ubr5	G	A	15: 37,980,076 (GRCm39)	A2434V	probably null	Het
Ugt1a9	A	G	1: 87,998,831 (GRCm39)	T94A	probably benign	Het
Vmn1r62	A	G	7: 5,678,182 (GRCm39)		probably benign	Het
Vmn2r61	T	C	7: 41,916,472 (GRCm39)	C362R	probably damaging	Het
Zfp354c	TCACACTCGGCACA	TCACA	11: 50,706,067 (GRCm39)		probably benign	Het
Zfp94	T	C	7: 24,003,107 (GRCm39)	K112E	probably benign	Het
Zfp954	A	T	7: 7,120,889 (GRCm39)	V47E	probably damaging	Het

Other mutations in Kras

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00808:Kras	APN	6	145,192,474 (GRCm39)	missense	probably damaging	1.00
IGL02929:Kras	APN	6	145,177,815 (GRCm39)	intron	probably benign
N/A - 293:Kras	UTSW	6	145,177,940 (GRCm39)	missense	probably benign	0.01
R1463:Kras	UTSW	6	145,170,787 (GRCm39)	intron	probably benign
R1518:Kras	UTSW	6	145,177,977 (GRCm39)	missense	probably benign	0.00
R1603:Kras	UTSW	6	145,170,871 (GRCm39)	nonsense	probably null
R1885:Kras	UTSW	6	145,177,843 (GRCm39)	missense	probably damaging	1.00
R5089:Kras	UTSW	6	145,170,869 (GRCm39)	missense	probably benign	0.00
R5133:Kras	UTSW	6	145,177,879 (GRCm39)	missense	probably benign	0.00
R7876:Kras	UTSW	6	145,170,848 (GRCm39)	missense	probably benign
R8151:Kras	UTSW	6	145,166,360 (GRCm39)	small deletion	probably benign
R8944:Kras	UTSW	6	145,170,853 (GRCm39)	missense	probably benign
R8951:Kras	UTSW	6	145,166,338 (GRCm39)	missense	probably benign
R9345:Kras	UTSW	6	145,192,442 (GRCm39)	missense	probably benign	0.00
Z1177:Kras	UTSW	6	145,192,498 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTTTAAGTCCAAAACCCAGGGAATAC -3'
(R):5'- AGTCTGTCATCTGAGCATGGC -3'

Sequencing Primer
(F):5'- GGGAATACTGGCACTTAGA -3'
(R):5'- TCATCTGAGCATGGCAAGCC -3'

Posted On

2019-11-12