Mutagenetix > Incidental Mutations

Incidental Mutation 'R7712:Zfp362'

594665

Institutional Source

Beutler Lab

Gene Symbol

Zfp362

Ensembl Gene

ENSMUSG00000028799

Gene Name

zinc finger protein 362

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.171) question?

Stock #

R7712 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

128773088-128806045 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 128777410 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 313 (H313Q)

Ref Sequence

ENSEMBL: ENSMUSP00000065733 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071108] [ENSMUST00000106072] [ENSMUST00000170934]

AlphaFold

B1ASA5

Predicted Effect

probably benign
Transcript: ENSMUST00000071108
AA Change: H313Q

PolyPhen 2 Score 0.143 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000065733
Gene: ENSMUSG00000028799
AA Change: H313Q

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000106072
AA Change: H313Q

PolyPhen 2 Score 0.143 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101686
Gene: ENSMUSG00000028799
AA Change: H313Q

Domain	Start	End	E-Value	Type
low complexity region	1	14	N/A	INTRINSIC
low complexity region	128	150	N/A	INTRINSIC
low complexity region	200	211	N/A	INTRINSIC
ZnF_C2H2	225	247	2.75e-3	SMART
ZnF_C2H2	253	275	1.98e-4	SMART
ZnF_C2H2	281	303	1.38e-3	SMART
ZnF_C2H2	309	333	3.49e-5	SMART
ZnF_C2H2	339	361	1.08e-1	SMART
ZnF_C2H2	369	391	7.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000170934
AA Change: H300Q

PolyPhen 2 Score 0.143 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000128499
Gene: ENSMUSG00000028799
AA Change: H300Q

Domain	Start	End	E-Value	Type
low complexity region	115	137	N/A	INTRINSIC
low complexity region	187	198	N/A	INTRINSIC
ZnF_C2H2	212	234	2.75e-3	SMART
ZnF_C2H2	240	262	1.98e-4	SMART
ZnF_C2H2	268	290	1.38e-3	SMART
ZnF_C2H2	296	320	3.49e-5	SMART
ZnF_C2H2	326	348	1.08e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	A	C	5: 114,165,738	D74A	probably benign	Het
Alox15	T	C	11: 70,350,253		probably null	Het
Ano5	A	G	7: 51,573,057	T455A	probably benign	Het
Ano5	T	G	7: 51,590,655	I827S	probably damaging	Het
Arid1a	G	A	4: 133,752,611	A334V	probably benign	Het
Atp13a4	A	G	16: 29,459,487	C298R		Het
Atp4a	T	A	7: 30,715,553	S256T	probably damaging	Het
Atp6v1c1	G	A	15: 38,686,805	V215I	probably benign	Het
Bbs9	T	A	9: 22,670,813	F600L	probably benign	Het
Bmp8b	A	T	4: 123,124,464	Y376F	possibly damaging	Het
Cacna2d1	A	T	5: 16,362,349	D986V	probably damaging	Het
Ccdc162	A	G	10: 41,627,227	F973S	possibly damaging	Het
Ccdc177	G	A	12: 80,757,938	Q521*	probably null	Het
Cd209b	T	A	8: 3,923,299	E158D	possibly damaging	Het
Cdk14	G	A	5: 5,380,061	T22I	possibly damaging	Het
Chl1	A	T	6: 103,711,102	I968F	possibly damaging	Het
Cnot7	T	C	8: 40,494,081	Y255C	probably damaging	Het
Col4a2	T	A	8: 11,425,376	L600H	probably benign	Het
Cpne7	C	A	8: 123,124,181	L129M	probably damaging	Het
Cpxm2	G	T	7: 132,154,378	P79Q	possibly damaging	Het
Dhx9	T	A	1: 153,465,001	N631I	probably benign	Het
Dmxl1	T	C	18: 49,893,461	S1879P	probably damaging	Het
Dnhd1	T	A	7: 105,651,624	F63I	probably benign	Het
Dok7	A	G	5: 35,066,522	N98S	probably damaging	Het
Fgd2	A	G	17: 29,376,912	T515A	probably benign	Het
Fmo1	T	C	1: 162,836,135	D275G	probably benign	Het
Gm5796	A	G	14: 4,034,759	Y90C	probably damaging	Het
H60b	C	A	10: 22,285,738	H42N	possibly damaging	Het
Hipk2	A	G	6: 38,703,634	S924P	probably benign	Het
Hpf1	C	A	8: 60,905,579	S27*	probably null	Het
Idua	T	G	5: 108,681,522	D443E	probably benign	Het
Lamc2	C	T	1: 153,133,611	G816D	possibly damaging	Het
Lgi3	T	A	14: 70,531,111	V16E	unknown	Het
Lpar1	T	A	4: 58,486,795	M159L	probably benign	Het
Magi2	A	G	5: 20,550,282	D618G	possibly damaging	Het
Man2b2	C	G	5: 36,810,314	Q903H	probably benign	Het
March7	A	T	2: 60,234,990	K537*	probably null	Het
Mcoln1	T	C	8: 3,505,873	F56S	probably damaging	Het
Myh7	C	G	14: 54,988,801	D461H	probably damaging	Het
Myo1b	A	T	1: 51,793,677	M383K	probably damaging	Het
Olfr160	T	C	9: 37,712,133	I49V	probably damaging	Het
Olfr161	T	C	16: 3,592,431	F12L	probably damaging	Het
Olfr360	A	T	2: 37,068,904	T200S	probably damaging	Het
Pars2	T	A	4: 106,654,079	Y353N	probably damaging	Het
Pcdha8	A	C	18: 36,992,684	D73A	possibly damaging	Het
Pcdhga8	C	T	18: 37,727,049	T386M	possibly damaging	Het
Pdzd2	G	T	15: 12,407,336	T346N	probably damaging	Het
Pik3c2b	A	G	1: 133,085,611	Q781R	probably damaging	Het
Pp2d1	T	A	17: 53,508,290	T469S	possibly damaging	Het
Sectm1a	G	A	11: 121,068,805	L166F	probably damaging	Het
Sgms1	T	A	19: 32,142,769	M246L	probably benign	Het
Shroom3	G	A	5: 92,950,947	G1429S	probably benign	Het
Snx17	T	A	5: 31,195,460	F101Y	probably damaging	Het
Sowahb	C	T	5: 93,043,381	S493N	probably benign	Het
Spc25	T	A	2: 69,206,137	R7S	unknown	Het
Sult2b1	A	G	7: 45,730,196	I308T	probably benign	Het
Tkt	A	G	14: 30,558,806	N65D	probably benign	Het
Ttc5	A	T	14: 50,773,312	S221T	probably benign	Het
Ubr5	G	A	15: 37,979,832	A2434V	probably null	Het
Wipf1	A	C	2: 73,444,461	S55R	probably damaging	Het
Zdbf2	T	C	1: 63,305,371	S970P	possibly damaging	Het
Zfp354c	TCACACTCGGCACA	TCACA	11: 50,815,240		probably benign	Het
Zfy2	T	A	Y: 2,121,420	I158F	probably benign	Het

Other mutations in Zfp362

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01667:Zfp362	APN	4	128787109	missense	probably damaging	1.00
IGL01939:Zfp362	APN	4	128787053	missense	probably benign
trek	UTSW	4	128785976	missense	probably damaging	1.00
Zax	UTSW	4	128790260	missense	possibly damaging	0.71
Zebulon	UTSW	4	128786015	missense	probably damaging	1.00
R1471:Zfp362	UTSW	4	128787200	missense	probably benign
R1892:Zfp362	UTSW	4	128790264	missense	probably benign	0.17
R1901:Zfp362	UTSW	4	128790276	missense	probably damaging	1.00
R2058:Zfp362	UTSW	4	128786987	missense	possibly damaging	0.71
R3739:Zfp362	UTSW	4	128786889	splice site	probably benign
R6772:Zfp362	UTSW	4	128790260	missense	possibly damaging	0.71
R6900:Zfp362	UTSW	4	128786015	missense	probably damaging	1.00
R6901:Zfp362	UTSW	4	128786015	missense	probably damaging	1.00
R7105:Zfp362	UTSW	4	128774526	missense	probably damaging	0.99
R7348:Zfp362	UTSW	4	128777217	missense	possibly damaging	0.88
R7629:Zfp362	UTSW	4	128786055	missense	probably damaging	1.00
R7763:Zfp362	UTSW	4	128787031	missense	probably benign
R8434:Zfp362	UTSW	4	128785976	missense	probably damaging	1.00
R8508:Zfp362	UTSW	4	128774606	missense	probably damaging	1.00
R9432:Zfp362	UTSW	4	128777187	missense	probably damaging	1.00
Z1177:Zfp362	UTSW	4	128774550	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CGCTGGTGAGACTGTAAAGAGC -3'
(R):5'- AACTGTGTTCAGACCAGCCG -3'

Sequencing Primer
(F):5'- CACGGTAAGGGGCTGTG -3'
(R):5'- GGCCTACTCTTGACCACCAGAG -3'

Posted On

2019-11-12