Mutagenetix > Incidental Mutations

Incidental Mutation 'R7716:Olfr304'

594918

Institutional Source

Beutler Lab

Gene Symbol

Olfr304

Ensembl Gene

ENSMUSG00000062426

Gene Name

olfactory receptor 304

Synonyms

GA_x6K02T2NHDJ-9721756-9722757, MOR219-3P

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.109) question?

Stock #

R7716 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

86385657-86386658 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 86386054 bp

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 202 (M202K)

Ref Sequence

ENSEMBL: ENSMUSP00000076449 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077210]

Predicted Effect

probably benign
Transcript: ENSMUST00000077210
AA Change: M202K

PolyPhen 2 Score 0.215 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000076449
Gene: ENSMUSG00000062426
AA Change: M202K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	8.9e-38	PFAM
Pfam:7tm_1	39	288	1.1e-21	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032F04Rik	G	A	3: 68,869,829	C41Y	possibly damaging	Het
3110062M04Rik	T	C	6: 34,875,581	N60D	possibly damaging	Het
Abcg1	A	G	17: 31,109,519	I421V	probably benign	Het
Ank2	G	T	3: 126,943,166	T3023K	unknown	Het
Asb5	T	A	8: 54,584,986	H181Q	probably benign	Het
Asnsd1	C	T	1: 53,347,743	V242I	probably benign	Het
Atp2a1	C	A	7: 126,462,187	G46V	possibly damaging	Het
Birc6	A	G	17: 74,562,061	S335G	probably damaging	Het
Bmp1	A	T	14: 70,477,922	Y924*	probably null	Het
Ccdc3	T	A	2: 5,138,302	F123L	probably benign	Het
Col6a6	C	A	9: 105,783,903	V336L	possibly damaging	Het
Cpe	T	C	8: 64,611,397	Y256C	probably damaging	Het
Creb1	T	A	1: 64,566,261	D62E	possibly damaging	Het
Cyp4a32	T	A	4: 115,601,086	Y38N	probably damaging	Het
Dnajc1	A	G	2: 18,219,873	S390P	probably benign	Het
E430018J23Rik	T	A	7: 127,392,087	M243L	probably benign	Het
Edrf1	G	A	7: 133,643,726	E198K	probably damaging	Het
Ehmt1	A	G	2: 24,884,499	S98P	probably damaging	Het
Emsy	G	A	7: 98,599,766	A812V	unknown	Het
Eppk1	A	T	15: 76,107,503	L1726*	probably null	Het
Flnb	G	T	14: 7,917,274	K1584N	possibly damaging	Het
Gclc	C	T	9: 77,754,927	R40W	probably damaging	Het
Gm5065	T	C	7: 5,359,820	I150T	possibly damaging	Het
Gnpat	A	G	8: 124,876,934	R194G	probably benign	Het
Gpd1	G	A	15: 99,722,086	S255N	probably damaging	Het
Il17re	T	C	6: 113,462,969		probably null	Het
Inpp5d	G	A	1: 87,665,399	V74I	probably damaging	Het
Iqcb1	T	C	16: 36,867,607	S443P	probably benign	Het
Irak2	AC	ACC	6: 113,690,898		probably null	Het
Kansl1l	A	G	1: 66,801,133	V336A	probably damaging	Het
Klhdc4	T	A	8: 121,829,420	M21L	unknown	Het
Lrguk	C	T	6: 34,095,539	A588V	probably damaging	Het
March5	T	C	19: 37,220,423	Y164H	probably benign	Het
Mbip	T	A	12: 56,345,688	H38L	probably benign	Het
Megf10	GGCAGCAACAGCACCAGCAGCAACAGCACCAGCAGCA	GGCAGCAACAGCACCAGCAGCA	18: 57,293,999		probably benign	Het
Myh8	A	T	11: 67,298,652	Q1218L	possibly damaging	Het
Ndc80	A	T	17: 71,523,594	I56K	probably benign	Het
Ndufs1	T	C	1: 63,152,857	D437G	possibly damaging	Het
Nlrc4	A	G	17: 74,446,656	L244P	probably damaging	Het
Olfr745	A	G	14: 50,642,358	T20A	probably benign	Het
Pdc	A	C	1: 150,330,783	D40A	probably benign	Het
Pdzd2	T	C	15: 12,373,374	Y2225C	possibly damaging	Het
Plce1	G	T	19: 38,716,851	G900V	probably benign	Het
Plpp1	A	T	13: 112,856,789	H86L	probably benign	Het
Plpp1	T	G	13: 112,859,652	F168C	probably damaging	Het
Pms1	G	A	1: 53,207,608	P312S	probably damaging	Het
Prf1	G	A	10: 61,300,155	R70H	possibly damaging	Het
Pygo1	A	G	9: 72,942,926	D19G	probably damaging	Het
Sdr42e1	T	A	8: 117,673,647		probably benign	Het
Serpinb8	C	T	1: 107,604,708	R177*	probably null	Het
Serpini1	A	C	3: 75,616,714	S210R	probably damaging	Het
Slc29a4	C	T	5: 142,718,506	P305L	probably benign	Het
Smco3	T	A	6: 136,831,249	Y209F	probably damaging	Het
Smtn	C	G	11: 3,524,708	S216T	probably benign	Het
Son	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	16: 91,656,691		probably benign	Het
Sri	T	A	5: 8,056,641		probably null	Het
Strn	G	A	17: 78,655,775	P716L	probably damaging	Het
Tmem167b	T	C	3: 108,558,897	Y69C	probably damaging	Het
Trpc7	A	T	13: 56,789,760	F628I	probably damaging	Het
Trrap	T	A	5: 144,777,146	I65N	possibly damaging	Het
Tspan14	C	A	14: 40,911,133	G201C	probably damaging	Het
Tti1	T	A	2: 158,000,698	I804F	probably benign	Het
Unc5b	C	T	10: 60,777,438	G340R	probably damaging	Het
Usp13	C	A	3: 32,837,856	Y61*	probably null	Het
Vmn1r230	T	C	17: 20,846,882	M111T	possibly damaging	Het
Vmn2r26	T	A	6: 124,061,745	Y760N	probably damaging	Het
Vmn2r87	A	G	10: 130,472,149	V740A	probably benign	Het
Zbtb14	A	G	17: 69,387,420	I38V	probably benign	Het
Zfp623	T	C	15: 75,948,422	I409T	probably damaging	Het

Other mutations in Olfr304

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Olfr304	APN	7	86386000	missense	possibly damaging	0.95
IGL02152:Olfr304	APN	7	86386043	missense	probably benign	0.00
IGL02540:Olfr304	APN	7	86386178	missense	possibly damaging	0.86
IGL03108:Olfr304	APN	7	86385721	missense	possibly damaging	0.95
IGL03374:Olfr304	APN	7	86386366	missense	probably damaging	1.00
R0040:Olfr304	UTSW	7	86386507	missense	probably benign	0.01
R0130:Olfr304	UTSW	7	86386306	missense	probably damaging	1.00
R0194:Olfr304	UTSW	7	86386374	nonsense	probably null
R0267:Olfr304	UTSW	7	86386267	missense	possibly damaging	0.64
R1026:Olfr304	UTSW	7	86386259	missense	probably damaging	0.98
R1865:Olfr304	UTSW	7	86386561	missense	probably damaging	1.00
R2090:Olfr304	UTSW	7	86386081	missense	probably benign	0.01
R3607:Olfr304	UTSW	7	86385695	missense	probably benign
R3861:Olfr304	UTSW	7	86386123	missense	possibly damaging	0.60
R3909:Olfr304	UTSW	7	86385974	missense	probably benign	0.05
R4113:Olfr304	UTSW	7	86386525	missense	possibly damaging	0.83
R5268:Olfr304	UTSW	7	86385659	makesense	probably null
R5649:Olfr304	UTSW	7	86386313	missense	probably damaging	1.00
R6343:Olfr304	UTSW	7	86385851	nonsense	probably null
X0054:Olfr304	UTSW	7	86385730	missense	probably benign	0.00
X0063:Olfr304	UTSW	7	86386492	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCAGCACAGTATACAGCACAG -3'
(R):5'- TCCTGGGCTACTGGAATTCTG -3'

Sequencing Primer
(F):5'- TATCTGAGGGAGGCTTCAGATAGAC -3'
(R):5'- CTGTTTGGAGCTTTATACACAGC -3'

Posted On

2019-11-12