Mutagenetix > Incidental Mutation

Incidental Mutation 'R7718:Mdn1'

595018

Institutional Source

Beutler Lab

Gene Symbol

Mdn1

Ensembl Gene

ENSMUSG00000058006

Gene Name

midasin AAA ATPase 1

Synonyms

4833432B22Rik, D4Abb1e, LOC213784

MMRRC Submission

045775-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7718 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

32657119-32775217 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 32718420 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 2225 (V2225A)

Ref Sequence

ENSEMBL: ENSMUSP00000071569 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071642] [ENSMUST00000178134]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000071642
AA Change: V2225A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006
AA Change: V2225A

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000178134
AA Change: V2225A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006
AA Change: V2225A

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Meta Mutation Damage Score

0.1825

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

99% (77/78)

Allele List at MGI

All alleles(29) : Targeted, other(2) Gene trapped(27)

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430033K04Rik	A	T	5: 138,646,122 (GRCm39)	H669L	possibly damaging	Het
Abcb1a	A	T	5: 8,765,788 (GRCm39)	N700I	probably damaging	Het
Abcc6	T	C	7: 45,626,816 (GRCm39)	K1414E	possibly damaging	Het
Adcy5	T	C	16: 35,100,785 (GRCm39)	V779A	probably benign	Het
Agap2	A	G	10: 126,915,734 (GRCm39)	S82G	possibly damaging	Het
Aldh1l1	A	G	6: 90,575,305 (GRCm39)	N864S	probably damaging	Het
Ang2	A	T	14: 51,433,217 (GRCm39)	V55E	probably benign	Het
Ank2	A	G	3: 126,758,662 (GRCm39)	M179T	possibly damaging	Het
Atrnl1	T	A	19: 57,728,615 (GRCm39)	C1090*	probably null	Het
Atxn1l	A	G	8: 110,459,866 (GRCm39)	L132P	probably damaging	Het
Bptf	A	G	11: 106,972,282 (GRCm39)	V862A	possibly damaging	Het
Capn11	C	A	17: 45,954,707 (GRCm39)	K143N	probably damaging	Het
Card6	A	G	15: 5,129,269 (GRCm39)	V709A	possibly damaging	Het
Cavin4	C	T	4: 48,671,984 (GRCm39)	A143V	probably benign	Het
Cntn5	A	G	9: 9,984,133 (GRCm39)	I160T	probably benign	Het
Cyp2c40	T	A	19: 39,755,782 (GRCm39)	N511Y	probably benign	Het
Dsc2	A	T	18: 20,174,835 (GRCm39)	I480N	probably damaging	Het
Elf2	G	T	3: 51,173,385 (GRCm39)		probably benign	Het
Enoph1	T	C	5: 100,210,019 (GRCm39)	V133A	possibly damaging	Het
Ezh2	A	T	6: 47,531,125 (GRCm39)	D186E	probably benign	Het
Fcgbpl1	T	C	7: 27,846,626 (GRCm39)	F1106S	probably damaging	Het
Gfra1	T	C	19: 58,441,889 (GRCm39)	D14G	possibly damaging	Het
Gmip	C	T	8: 70,270,383 (GRCm39)	R698W	probably damaging	Het
Grk4	A	G	5: 34,852,160 (GRCm39)	N135D	probably benign	Het
Hspb6	A	G	7: 30,253,772 (GRCm39)	D95G	probably benign	Het
Htatip2	T	G	7: 49,420,632 (GRCm39)	H159Q	possibly damaging	Het
Igfn1	T	C	1: 135,896,774 (GRCm39)	E1264G	probably benign	Het
Insyn2b	T	C	11: 34,352,539 (GRCm39)	S194P	probably benign	Het
Katnb1	T	A	8: 95,821,836 (GRCm39)	V223E	possibly damaging	Het
Klra6	AGG	AG	6: 129,990,315 (GRCm39)		probably null	Het
Masp2	C	T	4: 148,687,204 (GRCm39)	R29C	probably damaging	Het
Mcm3	A	T	1: 20,887,498 (GRCm39)	C123*	probably null	Het
Me1	A	G	9: 86,561,953 (GRCm39)	L44S	probably damaging	Het
Mlxip	A	G	5: 123,583,577 (GRCm39)	N380S	probably benign	Het
Muc21	T	C	17: 35,933,728 (GRCm39)	T153A	unknown	Het
Myh14	C	T	7: 44,310,464 (GRCm39)	V140I	probably damaging	Het
Myocd	A	T	11: 65,109,452 (GRCm39)	D106E	probably damaging	Het
Oat	T	C	7: 132,159,988 (GRCm39)	I411V	probably benign	Het
Or2d2	A	T	7: 106,727,925 (GRCm39)	V225D	probably damaging	Het
Or5p59	T	C	7: 107,702,855 (GRCm39)	V113A	probably benign	Het
Or8k38	A	G	2: 86,488,373 (GRCm39)	V143A	probably benign	Het
Orc2	A	T	1: 58,519,476 (GRCm39)	H246Q	possibly damaging	Het
Pank4	A	G	4: 155,059,100 (GRCm39)	E411G	probably damaging	Het
Patl2	A	T	2: 121,957,255 (GRCm39)		probably null	Het
Pcdhb15	A	G	18: 37,608,216 (GRCm39)	N483D	probably damaging	Het
Pcdhb20	A	G	18: 37,638,704 (GRCm39)	D410G	probably damaging	Het
Pdcl2	A	G	5: 76,465,846 (GRCm39)	C125R	probably damaging	Het
Peli3	C	G	19: 4,984,584 (GRCm39)		probably null	Het
Pkd1	A	G	17: 24,805,474 (GRCm39)	D3313G	probably benign	Het
Plec	A	C	15: 76,061,639 (GRCm39)	M2766R	probably damaging	Het
Ppargc1a	C	T	5: 51,655,504 (GRCm39)	V99M	probably damaging	Het
Prr35	T	A	17: 26,165,998 (GRCm39)	R430*	probably null	Het
Psg19	G	A	7: 18,526,368 (GRCm39)	A374V	probably benign	Het
Psmd7	A	T	8: 108,313,261 (GRCm39)	F54L	possibly damaging	Het
Ptbp2	C	T	3: 119,514,637 (GRCm39)	G397R	probably null	Het
Ranbp3	A	G	17: 57,003,718 (GRCm39)	D39G	probably damaging	Het
Rcor3	T	C	1: 191,786,021 (GRCm39)	T406A	probably benign	Het
Rhbdl2	T	A	4: 123,718,712 (GRCm39)	I222K	probably damaging	Het
Ripk2	T	C	4: 16,151,968 (GRCm39)	N197S	possibly damaging	Het
Rpia	A	T	6: 70,743,602 (GRCm39)	M283K	probably damaging	Het
Rps6kc1	T	C	1: 190,604,022 (GRCm39)	D200G	probably benign	Het
Sipa1l1	A	T	12: 82,389,271 (GRCm39)	K499M	probably damaging	Het
Slc5a4b	A	G	10: 75,906,407 (GRCm39)	L404P	probably damaging	Het
Son	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	16: 91,457,222 (GRCm39)		probably benign	Het
Spmip4	G	A	6: 50,566,078 (GRCm39)		probably null	Het
St7	A	C	6: 17,854,998 (GRCm39)	T312P	probably damaging	Het
Strbp	T	C	2: 37,515,294 (GRCm39)	E244G	probably damaging	Het
Tbc1d8	T	G	1: 39,416,061 (GRCm39)	T871P	probably benign	Het
Tcp1	T	A	17: 13,141,049 (GRCm39)	I286N	probably damaging	Het
Tead3	C	A	17: 28,552,491 (GRCm39)	V327F	probably damaging	Het
Tmem132c	A	T	5: 127,640,504 (GRCm39)	T892S	probably benign	Het
Trmt2a	T	C	16: 18,068,487 (GRCm39)	S65P	probably benign	Het
Ubp1	A	G	9: 113,802,597 (GRCm39)	N479S	possibly damaging	Het
Ubtfl1	A	T	9: 18,320,527 (GRCm39)	L18F	possibly damaging	Het
Uevld	T	G	7: 46,587,804 (GRCm39)	M299L	probably benign	Het
Unc13b	T	A	4: 43,173,854 (GRCm39)	Y1561N	unknown	Het
Ylpm1	A	G	12: 85,075,896 (GRCm39)	K874E	probably damaging	Het
Zbtb37	G	A	1: 160,859,802 (GRCm39)	R168W	possibly damaging	Het

Other mutations in Mdn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Mdn1	APN	4	32,723,651 (GRCm39)	missense	probably damaging	1.00
IGL00426:Mdn1	APN	4	32,719,214 (GRCm39)	missense	possibly damaging	0.91
IGL00570:Mdn1	APN	4	32,735,719 (GRCm39)	missense	probably benign
IGL00573:Mdn1	APN	4	32,666,619 (GRCm39)	critical splice donor site	probably null
IGL00983:Mdn1	APN	4	32,735,525 (GRCm39)	missense	probably damaging	1.00
IGL01288:Mdn1	APN	4	32,730,864 (GRCm39)	missense	probably benign	0.00
IGL01359:Mdn1	APN	4	32,743,686 (GRCm39)	missense	probably benign	0.10
IGL01457:Mdn1	APN	4	32,715,922 (GRCm39)	missense	possibly damaging	0.82
IGL01530:Mdn1	APN	4	32,711,938 (GRCm39)	splice site	probably benign
IGL01684:Mdn1	APN	4	32,726,857 (GRCm39)	missense	probably benign
IGL01753:Mdn1	APN	4	32,708,483 (GRCm39)	missense	probably benign
IGL01901:Mdn1	APN	4	32,669,591 (GRCm39)	missense	probably damaging	1.00
IGL01952:Mdn1	APN	4	32,723,657 (GRCm39)	missense	possibly damaging	0.82
IGL01960:Mdn1	APN	4	32,758,393 (GRCm39)	missense	probably benign	0.14
IGL02019:Mdn1	APN	4	32,749,948 (GRCm39)	missense	possibly damaging	0.93
IGL02100:Mdn1	APN	4	32,715,708 (GRCm39)	missense	possibly damaging	0.90
IGL02117:Mdn1	APN	4	32,709,364 (GRCm39)	missense	probably benign	0.00
IGL02154:Mdn1	APN	4	32,740,395 (GRCm39)	missense	probably benign	0.35
IGL02216:Mdn1	APN	4	32,739,092 (GRCm39)	missense	probably benign	0.03
IGL02371:Mdn1	APN	4	32,676,860 (GRCm39)	splice site	probably benign
IGL02396:Mdn1	APN	4	32,700,120 (GRCm39)	missense	probably damaging	0.99
IGL02454:Mdn1	APN	4	32,694,674 (GRCm39)	critical splice donor site	probably null
IGL02502:Mdn1	APN	4	32,670,579 (GRCm39)	missense	possibly damaging	0.69
IGL02883:Mdn1	APN	4	32,763,199 (GRCm39)	missense	probably benign	0.05
IGL02946:Mdn1	APN	4	32,734,366 (GRCm39)	missense	probably damaging	0.98
IGL02950:Mdn1	APN	4	32,713,360 (GRCm39)	splice site	probably benign
IGL03076:Mdn1	APN	4	32,735,564 (GRCm39)	missense	probably damaging	0.97
IGL03129:Mdn1	APN	4	32,729,994 (GRCm39)	missense	possibly damaging	0.47
IGL03234:Mdn1	APN	4	32,732,842 (GRCm39)	missense	probably benign	0.06
3-1:Mdn1	UTSW	4	32,725,967 (GRCm39)	critical splice donor site	probably null
IGL03046:Mdn1	UTSW	4	32,694,495 (GRCm39)	missense	possibly damaging	0.73
P0035:Mdn1	UTSW	4	32,749,934 (GRCm39)	missense	probably benign	0.05
PIT4508001:Mdn1	UTSW	4	32,719,223 (GRCm39)	missense	probably damaging	0.97
PIT4618001:Mdn1	UTSW	4	32,746,527 (GRCm39)	missense	probably benign	0.20
R0008:Mdn1	UTSW	4	32,718,317 (GRCm39)	missense	possibly damaging	0.47
R0110:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0125:Mdn1	UTSW	4	32,729,956 (GRCm39)	missense	probably damaging	0.98
R0257:Mdn1	UTSW	4	32,693,534 (GRCm39)	missense	probably damaging	0.99
R0266:Mdn1	UTSW	4	32,741,835 (GRCm39)	missense	probably damaging	0.99
R0349:Mdn1	UTSW	4	32,750,318 (GRCm39)	missense	probably damaging	1.00
R0362:Mdn1	UTSW	4	32,746,439 (GRCm39)	critical splice acceptor site	probably null
R0421:Mdn1	UTSW	4	32,684,707 (GRCm39)	missense	probably benign	0.39
R0450:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0465:Mdn1	UTSW	4	32,699,204 (GRCm39)	splice site	probably benign
R0469:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0477:Mdn1	UTSW	4	32,750,928 (GRCm39)	missense	probably benign	0.02
R0481:Mdn1	UTSW	4	32,767,182 (GRCm39)	splice site	probably benign
R0504:Mdn1	UTSW	4	32,698,916 (GRCm39)	splice site	probably benign
R0522:Mdn1	UTSW	4	32,672,837 (GRCm39)	missense	probably benign	0.09
R0550:Mdn1	UTSW	4	32,730,479 (GRCm39)	missense	probably benign	0.13
R0607:Mdn1	UTSW	4	32,732,829 (GRCm39)	missense	probably benign	0.36
R0607:Mdn1	UTSW	4	32,712,014 (GRCm39)	missense	probably damaging	1.00
R0664:Mdn1	UTSW	4	32,768,011 (GRCm39)	nonsense	probably null
R0701:Mdn1	UTSW	4	32,699,263 (GRCm39)	missense	probably benign	0.00
R0801:Mdn1	UTSW	4	32,668,895 (GRCm39)	missense	probably benign	0.04
R0841:Mdn1	UTSW	4	32,752,032 (GRCm39)	missense	probably benign	0.23
R0849:Mdn1	UTSW	4	32,741,835 (GRCm39)	missense	probably damaging	0.99
R0893:Mdn1	UTSW	4	32,701,713 (GRCm39)	missense	probably benign	0.01
R1114:Mdn1	UTSW	4	32,746,568 (GRCm39)	critical splice donor site	probably null
R1137:Mdn1	UTSW	4	32,694,511 (GRCm39)	missense	probably damaging	1.00
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1257:Mdn1	UTSW	4	32,667,089 (GRCm39)	critical splice acceptor site	probably null
R1356:Mdn1	UTSW	4	32,700,334 (GRCm39)	splice site	probably benign
R1466:Mdn1	UTSW	4	32,730,788 (GRCm39)	missense	probably benign	0.28
R1466:Mdn1	UTSW	4	32,730,788 (GRCm39)	missense	probably benign	0.28
R1518:Mdn1	UTSW	4	32,739,977 (GRCm39)	missense	probably damaging	1.00
R1569:Mdn1	UTSW	4	32,723,501 (GRCm39)	missense	probably null	0.10
R1574:Mdn1	UTSW	4	32,722,315 (GRCm39)	missense	probably benign
R1574:Mdn1	UTSW	4	32,722,315 (GRCm39)	missense	probably benign
R1591:Mdn1	UTSW	4	32,700,092 (GRCm39)	missense	possibly damaging	0.65
R1678:Mdn1	UTSW	4	32,663,050 (GRCm39)	missense	probably damaging	0.99
R1696:Mdn1	UTSW	4	32,700,417 (GRCm39)	missense	possibly damaging	0.91
R1707:Mdn1	UTSW	4	32,693,504 (GRCm39)	missense	probably damaging	1.00
R1749:Mdn1	UTSW	4	32,773,952 (GRCm39)	missense	probably damaging	1.00
R1780:Mdn1	UTSW	4	32,700,103 (GRCm39)	missense	probably damaging	1.00
R1833:Mdn1	UTSW	4	32,720,761 (GRCm39)	missense	probably damaging	0.97
R1858:Mdn1	UTSW	4	32,730,881 (GRCm39)	missense	probably benign	0.17
R1870:Mdn1	UTSW	4	32,763,339 (GRCm39)	missense	probably damaging	1.00
R1887:Mdn1	UTSW	4	32,742,540 (GRCm39)	missense	probably damaging	1.00
R1909:Mdn1	UTSW	4	32,760,839 (GRCm39)	small deletion	probably benign
R2075:Mdn1	UTSW	4	32,716,058 (GRCm39)	missense	probably benign	0.03
R2103:Mdn1	UTSW	4	32,738,712 (GRCm39)	missense	possibly damaging	0.75
R2104:Mdn1	UTSW	4	32,743,843 (GRCm39)	splice site	probably null
R2110:Mdn1	UTSW	4	32,700,409 (GRCm39)	missense	probably damaging	1.00
R2111:Mdn1	UTSW	4	32,700,409 (GRCm39)	missense	probably damaging	1.00
R2206:Mdn1	UTSW	4	32,716,271 (GRCm39)	missense	possibly damaging	0.71
R2221:Mdn1	UTSW	4	32,763,306 (GRCm39)	missense	probably benign	0.37
R2240:Mdn1	UTSW	4	32,765,701 (GRCm39)	missense	possibly damaging	0.90
R2351:Mdn1	UTSW	4	32,750,010 (GRCm39)	missense	probably benign	0.21
R2421:Mdn1	UTSW	4	32,723,621 (GRCm39)	missense	probably damaging	0.96
R3036:Mdn1	UTSW	4	32,750,013 (GRCm39)	missense	probably damaging	0.99
R3434:Mdn1	UTSW	4	32,733,726 (GRCm39)	critical splice donor site	probably null
R3435:Mdn1	UTSW	4	32,733,726 (GRCm39)	critical splice donor site	probably null
R3783:Mdn1	UTSW	4	32,720,818 (GRCm39)	missense	probably benign	0.01
R3811:Mdn1	UTSW	4	32,693,506 (GRCm39)	nonsense	probably null
R3973:Mdn1	UTSW	4	32,722,363 (GRCm39)	missense	probably benign	0.00
R4154:Mdn1	UTSW	4	32,707,475 (GRCm39)	missense	probably damaging	0.96
R4372:Mdn1	UTSW	4	32,743,809 (GRCm39)	missense	probably benign	0.03
R4393:Mdn1	UTSW	4	32,754,482 (GRCm39)	missense	possibly damaging	0.48
R4438:Mdn1	UTSW	4	32,704,635 (GRCm39)	missense	probably damaging	1.00
R4471:Mdn1	UTSW	4	32,668,860 (GRCm39)	missense	probably benign	0.00
R4509:Mdn1	UTSW	4	32,715,883 (GRCm39)	missense	probably damaging	1.00
R4538:Mdn1	UTSW	4	32,722,334 (GRCm39)	missense	probably damaging	1.00
R4557:Mdn1	UTSW	4	32,754,437 (GRCm39)	missense	probably damaging	1.00
R4570:Mdn1	UTSW	4	32,741,812 (GRCm39)	missense	probably damaging	1.00
R4591:Mdn1	UTSW	4	32,707,636 (GRCm39)	missense	probably damaging	1.00
R4658:Mdn1	UTSW	4	32,730,749 (GRCm39)	splice site	probably null
R4667:Mdn1	UTSW	4	32,679,572 (GRCm39)	missense	probably damaging	1.00
R4684:Mdn1	UTSW	4	32,666,430 (GRCm39)	missense	probably damaging	1.00
R4778:Mdn1	UTSW	4	32,683,583 (GRCm39)	nonsense	probably null
R4807:Mdn1	UTSW	4	32,685,651 (GRCm39)	splice site	probably null
R4923:Mdn1	UTSW	4	32,671,608 (GRCm39)	missense	possibly damaging	0.89
R4951:Mdn1	UTSW	4	32,707,459 (GRCm39)	missense	probably damaging	1.00
R4963:Mdn1	UTSW	4	32,756,512 (GRCm39)	missense	probably benign	0.00
R4971:Mdn1	UTSW	4	32,739,827 (GRCm39)	missense	probably damaging	1.00
R4973:Mdn1	UTSW	4	32,734,418 (GRCm39)	missense	probably benign	0.01
R5122:Mdn1	UTSW	4	32,670,593 (GRCm39)	missense	probably damaging	1.00
R5159:Mdn1	UTSW	4	32,774,008 (GRCm39)	missense	possibly damaging	0.93
R5164:Mdn1	UTSW	4	32,759,011 (GRCm39)	splice site	probably null
R5215:Mdn1	UTSW	4	32,741,418 (GRCm39)	missense	possibly damaging	0.78
R5217:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5219:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5365:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5366:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5368:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5445:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5462:Mdn1	UTSW	4	32,720,897 (GRCm39)	missense	probably benign
R5522:Mdn1	UTSW	4	32,685,783 (GRCm39)	missense	probably damaging	1.00
R5525:Mdn1	UTSW	4	32,767,961 (GRCm39)	missense	possibly damaging	0.73
R5578:Mdn1	UTSW	4	32,728,167 (GRCm39)	missense	probably benign	0.04
R5605:Mdn1	UTSW	4	32,765,664 (GRCm39)	missense	probably benign
R5621:Mdn1	UTSW	4	32,716,371 (GRCm39)	missense	possibly damaging	0.46
R5636:Mdn1	UTSW	4	32,695,480 (GRCm39)	missense	probably damaging	1.00
R5650:Mdn1	UTSW	4	32,667,467 (GRCm39)	splice site	probably null
R5780:Mdn1	UTSW	4	32,722,950 (GRCm39)	missense	probably benign	0.02
R5838:Mdn1	UTSW	4	32,754,547 (GRCm39)	missense	probably damaging	0.99
R5857:Mdn1	UTSW	4	32,670,646 (GRCm39)	missense	probably benign	0.09
R5895:Mdn1	UTSW	4	32,695,400 (GRCm39)	missense	probably damaging	1.00
R5943:Mdn1	UTSW	4	32,678,330 (GRCm39)	missense	probably damaging	1.00
R6008:Mdn1	UTSW	4	32,741,073 (GRCm39)	missense	probably damaging	1.00
R6013:Mdn1	UTSW	4	32,715,713 (GRCm39)	missense	probably damaging	1.00
R6075:Mdn1	UTSW	4	32,689,581 (GRCm39)	missense	possibly damaging	0.48
R6151:Mdn1	UTSW	4	32,684,735 (GRCm39)	missense	probably damaging	1.00
R6163:Mdn1	UTSW	4	32,716,040 (GRCm39)	missense	probably damaging	1.00
R6181:Mdn1	UTSW	4	32,715,953 (GRCm39)	missense	probably damaging	1.00
R6211:Mdn1	UTSW	4	32,696,269 (GRCm39)	missense	probably benign	0.12
R6249:Mdn1	UTSW	4	32,708,484 (GRCm39)	missense	possibly damaging	0.85
R6251:Mdn1	UTSW	4	32,748,590 (GRCm39)	missense	probably benign	0.13
R6253:Mdn1	UTSW	4	32,749,593 (GRCm39)	missense	probably benign	0.25
R6273:Mdn1	UTSW	4	32,715,979 (GRCm39)	missense	probably benign	0.01
R6297:Mdn1	UTSW	4	32,730,054 (GRCm39)	nonsense	probably null
R6384:Mdn1	UTSW	4	32,670,607 (GRCm39)	missense	probably damaging	1.00
R6463:Mdn1	UTSW	4	32,773,308 (GRCm39)	missense	probably damaging	1.00
R6528:Mdn1	UTSW	4	32,713,780 (GRCm39)	missense	probably damaging	1.00
R6688:Mdn1	UTSW	4	32,774,041 (GRCm39)	missense	possibly damaging	0.74
R6762:Mdn1	UTSW	4	32,676,786 (GRCm39)	missense	possibly damaging	0.50
R6794:Mdn1	UTSW	4	32,741,893 (GRCm39)	missense	probably damaging	1.00
R6894:Mdn1	UTSW	4	32,748,614 (GRCm39)	missense	possibly damaging	0.75
R6935:Mdn1	UTSW	4	32,774,041 (GRCm39)	missense	possibly damaging	0.74
R6980:Mdn1	UTSW	4	32,726,942 (GRCm39)	critical splice donor site	probably null
R6995:Mdn1	UTSW	4	32,733,374 (GRCm39)	missense	probably benign	0.03
R7048:Mdn1	UTSW	4	32,767,969 (GRCm39)	missense	probably benign	0.00
R7082:Mdn1	UTSW	4	32,762,341 (GRCm39)	missense	probably benign
R7158:Mdn1	UTSW	4	32,725,121 (GRCm39)	missense	probably benign	0.09
R7166:Mdn1	UTSW	4	32,746,446 (GRCm39)	missense	probably damaging	1.00
R7168:Mdn1	UTSW	4	32,719,184 (GRCm39)	missense	probably damaging	1.00
R7175:Mdn1	UTSW	4	32,694,634 (GRCm39)	missense	probably damaging	1.00
R7195:Mdn1	UTSW	4	32,701,823 (GRCm39)	missense	probably damaging	1.00
R7250:Mdn1	UTSW	4	32,695,427 (GRCm39)	missense	probably damaging	1.00
R7274:Mdn1	UTSW	4	32,725,944 (GRCm39)	missense	probably benign	0.12
R7330:Mdn1	UTSW	4	32,723,685 (GRCm39)	missense	probably benign	0.16
R7363:Mdn1	UTSW	4	32,691,729 (GRCm39)	missense	probably damaging	0.99
R7369:Mdn1	UTSW	4	32,773,375 (GRCm39)	missense	probably damaging	0.99
R7452:Mdn1	UTSW	4	32,739,030 (GRCm39)	missense	possibly damaging	0.87
R7523:Mdn1	UTSW	4	32,667,270 (GRCm39)	critical splice acceptor site	probably null
R7594:Mdn1	UTSW	4	32,696,359 (GRCm39)	missense	probably benign	0.27
R7605:Mdn1	UTSW	4	32,694,599 (GRCm39)	missense	probably damaging	1.00
R7661:Mdn1	UTSW	4	32,691,229 (GRCm39)	missense	probably benign	0.08
R7689:Mdn1	UTSW	4	32,739,912 (GRCm39)	missense	probably damaging	1.00
R7699:Mdn1	UTSW	4	32,741,344 (GRCm39)	missense	probably damaging	1.00
R7700:Mdn1	UTSW	4	32,741,344 (GRCm39)	missense	probably damaging	1.00
R7714:Mdn1	UTSW	4	32,722,360 (GRCm39)	missense	possibly damaging	0.75
R7762:Mdn1	UTSW	4	32,734,421 (GRCm39)	missense	probably benign
R7787:Mdn1	UTSW	4	32,741,794 (GRCm39)	missense	probably damaging	1.00
R8111:Mdn1	UTSW	4	32,674,003 (GRCm39)	missense	possibly damaging	0.81
R8222:Mdn1	UTSW	4	32,707,477 (GRCm39)	missense	probably benign	0.09
R8246:Mdn1	UTSW	4	32,657,284 (GRCm39)	missense	probably benign	0.06
R8267:Mdn1	UTSW	4	32,742,485 (GRCm39)	missense	possibly damaging	0.82
R8286:Mdn1	UTSW	4	32,731,960 (GRCm39)	missense	possibly damaging	0.91
R8305:Mdn1	UTSW	4	32,725,107 (GRCm39)	missense	probably benign
R8318:Mdn1	UTSW	4	32,735,897 (GRCm39)	critical splice donor site	probably null
R8379:Mdn1	UTSW	4	32,756,453 (GRCm39)	missense	probably null	1.00
R8384:Mdn1	UTSW	4	32,765,680 (GRCm39)	missense	probably benign	0.05
R8514:Mdn1	UTSW	4	32,739,857 (GRCm39)	missense	probably damaging	1.00
R8560:Mdn1	UTSW	4	32,743,830 (GRCm39)	missense	probably benign	0.08
R8672:Mdn1	UTSW	4	32,768,793 (GRCm39)	missense	probably damaging	1.00
R8708:Mdn1	UTSW	4	32,725,854 (GRCm39)	missense	probably damaging	1.00
R8769:Mdn1	UTSW	4	32,751,390 (GRCm39)	missense	probably damaging	0.97
R8896:Mdn1	UTSW	4	32,678,328 (GRCm39)	missense	probably benign	0.28
R8918:Mdn1	UTSW	4	32,744,579 (GRCm39)	nonsense	probably null
R8920:Mdn1	UTSW	4	32,719,280 (GRCm39)	missense	probably damaging	1.00
R8966:Mdn1	UTSW	4	32,672,837 (GRCm39)	nonsense	probably null
R8997:Mdn1	UTSW	4	32,773,275 (GRCm39)	missense	probably damaging	1.00
R9120:Mdn1	UTSW	4	32,701,814 (GRCm39)	missense	probably damaging	1.00
R9129:Mdn1	UTSW	4	32,676,812 (GRCm39)	missense	probably benign	0.24
R9131:Mdn1	UTSW	4	32,762,275 (GRCm39)	missense	possibly damaging	0.69
R9200:Mdn1	UTSW	4	32,760,791 (GRCm39)	missense	probably benign	0.00
R9226:Mdn1	UTSW	4	32,694,612 (GRCm39)	missense	probably benign	0.25
R9235:Mdn1	UTSW	4	32,739,122 (GRCm39)	missense	probably benign	0.10
R9293:Mdn1	UTSW	4	32,707,579 (GRCm39)	missense	probably damaging	1.00
R9315:Mdn1	UTSW	4	32,760,911 (GRCm39)	missense	probably benign	0.00
R9338:Mdn1	UTSW	4	32,666,536 (GRCm39)	missense	probably benign	0.00
R9353:Mdn1	UTSW	4	32,693,504 (GRCm39)	missense	probably damaging	1.00
R9393:Mdn1	UTSW	4	32,713,825 (GRCm39)	missense
R9420:Mdn1	UTSW	4	32,678,414 (GRCm39)	missense	probably damaging	1.00
R9475:Mdn1	UTSW	4	32,739,849 (GRCm39)	missense	possibly damaging	0.65
R9583:Mdn1	UTSW	4	32,741,372 (GRCm39)	missense	probably damaging	1.00
R9600:Mdn1	UTSW	4	32,684,723 (GRCm39)	nonsense	probably null
R9640:Mdn1	UTSW	4	32,754,539 (GRCm39)	missense	probably damaging	1.00
R9688:Mdn1	UTSW	4	32,745,590 (GRCm39)	missense	probably damaging	1.00
R9744:Mdn1	UTSW	4	32,715,711 (GRCm39)	missense	possibly damaging	0.91
X0066:Mdn1	UTSW	4	32,739,030 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,696,244 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,668,944 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,667,102 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TCAGCTTTGTCTCACAAGTGTTG -3'
(R):5'- GTCTAAGAATGGCTGGAGATGC -3'

Sequencing Primer
(F):5'- GTCTCACAAGTGTTGGGATTTG -3'
(R):5'- TGGCTGGAGATGCAACGC -3'

Posted On

2019-11-12