Incidental Mutation 'R7718:Grk4'
ID 595025
Institutional Source Beutler Lab
Gene Symbol Grk4
Ensembl Gene ENSMUSG00000052783
Gene Name G protein-coupled receptor kinase 4
Synonyms Gprk2l, A830025H08Rik
MMRRC Submission 045775-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.148) question?
Stock # R7718 (G1)
Quality Score 225.009
Status Validated
Chromosome 5
Chromosomal Location 34817723-34912649 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 34852160 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Aspartic acid at position 135 (N135D)
Ref Sequence ENSEMBL: ENSMUSP00000001112 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000001112] [ENSMUST00000074651]
AlphaFold O70291
Predicted Effect probably benign
Transcript: ENSMUST00000001112
AA Change: N135D

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000001112
Gene: ENSMUSG00000052783
AA Change: N135D

DomainStartEndE-ValueType
RGS 51 171 1.61e-31 SMART
S_TKc 186 448 7.78e-85 SMART
S_TK_X 449 528 2.98e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000074651
AA Change: N135D

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000074223
Gene: ENSMUSG00000052783
AA Change: N135D

DomainStartEndE-ValueType
RGS 51 163 1.39e-12 SMART
Meta Mutation Damage Score 0.0846 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 99% (77/78)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
PHENOTYPE: Mice heterozygous for a knock-out allele are viable, fertile and overtly normal. [provided by MGI curators]
Allele List at MGI

All alleles(4) : Targeted, knock-out(1) Targeted, other(1) Gene trapped(2)

Other mutations in this stock
Total: 78 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A430033K04Rik A T 5: 138,646,122 (GRCm39) H669L possibly damaging Het
Abcb1a A T 5: 8,765,788 (GRCm39) N700I probably damaging Het
Abcc6 T C 7: 45,626,816 (GRCm39) K1414E possibly damaging Het
Adcy5 T C 16: 35,100,785 (GRCm39) V779A probably benign Het
Agap2 A G 10: 126,915,734 (GRCm39) S82G possibly damaging Het
Aldh1l1 A G 6: 90,575,305 (GRCm39) N864S probably damaging Het
Ang2 A T 14: 51,433,217 (GRCm39) V55E probably benign Het
Ank2 A G 3: 126,758,662 (GRCm39) M179T possibly damaging Het
Atrnl1 T A 19: 57,728,615 (GRCm39) C1090* probably null Het
Atxn1l A G 8: 110,459,866 (GRCm39) L132P probably damaging Het
Bptf A G 11: 106,972,282 (GRCm39) V862A possibly damaging Het
Capn11 C A 17: 45,954,707 (GRCm39) K143N probably damaging Het
Card6 A G 15: 5,129,269 (GRCm39) V709A possibly damaging Het
Cavin4 C T 4: 48,671,984 (GRCm39) A143V probably benign Het
Cntn5 A G 9: 9,984,133 (GRCm39) I160T probably benign Het
Cyp2c40 T A 19: 39,755,782 (GRCm39) N511Y probably benign Het
Dsc2 A T 18: 20,174,835 (GRCm39) I480N probably damaging Het
Elf2 G T 3: 51,173,385 (GRCm39) probably benign Het
Enoph1 T C 5: 100,210,019 (GRCm39) V133A possibly damaging Het
Ezh2 A T 6: 47,531,125 (GRCm39) D186E probably benign Het
Fcgbpl1 T C 7: 27,846,626 (GRCm39) F1106S probably damaging Het
Gfra1 T C 19: 58,441,889 (GRCm39) D14G possibly damaging Het
Gmip C T 8: 70,270,383 (GRCm39) R698W probably damaging Het
Hspb6 A G 7: 30,253,772 (GRCm39) D95G probably benign Het
Htatip2 T G 7: 49,420,632 (GRCm39) H159Q possibly damaging Het
Igfn1 T C 1: 135,896,774 (GRCm39) E1264G probably benign Het
Insyn2b T C 11: 34,352,539 (GRCm39) S194P probably benign Het
Katnb1 T A 8: 95,821,836 (GRCm39) V223E possibly damaging Het
Klra6 AGG AG 6: 129,990,315 (GRCm39) probably null Het
Masp2 C T 4: 148,687,204 (GRCm39) R29C probably damaging Het
Mcm3 A T 1: 20,887,498 (GRCm39) C123* probably null Het
Mdn1 T C 4: 32,718,420 (GRCm39) V2225A probably damaging Het
Me1 A G 9: 86,561,953 (GRCm39) L44S probably damaging Het
Mlxip A G 5: 123,583,577 (GRCm39) N380S probably benign Het
Muc21 T C 17: 35,933,728 (GRCm39) T153A unknown Het
Myh14 C T 7: 44,310,464 (GRCm39) V140I probably damaging Het
Myocd A T 11: 65,109,452 (GRCm39) D106E probably damaging Het
Oat T C 7: 132,159,988 (GRCm39) I411V probably benign Het
Or2d2 A T 7: 106,727,925 (GRCm39) V225D probably damaging Het
Or5p59 T C 7: 107,702,855 (GRCm39) V113A probably benign Het
Or8k38 A G 2: 86,488,373 (GRCm39) V143A probably benign Het
Orc2 A T 1: 58,519,476 (GRCm39) H246Q possibly damaging Het
Pank4 A G 4: 155,059,100 (GRCm39) E411G probably damaging Het
Patl2 A T 2: 121,957,255 (GRCm39) probably null Het
Pcdhb15 A G 18: 37,608,216 (GRCm39) N483D probably damaging Het
Pcdhb20 A G 18: 37,638,704 (GRCm39) D410G probably damaging Het
Pdcl2 A G 5: 76,465,846 (GRCm39) C125R probably damaging Het
Peli3 C G 19: 4,984,584 (GRCm39) probably null Het
Pkd1 A G 17: 24,805,474 (GRCm39) D3313G probably benign Het
Plec A C 15: 76,061,639 (GRCm39) M2766R probably damaging Het
Ppargc1a C T 5: 51,655,504 (GRCm39) V99M probably damaging Het
Prr35 T A 17: 26,165,998 (GRCm39) R430* probably null Het
Psg19 G A 7: 18,526,368 (GRCm39) A374V probably benign Het
Psmd7 A T 8: 108,313,261 (GRCm39) F54L possibly damaging Het
Ptbp2 C T 3: 119,514,637 (GRCm39) G397R probably null Het
Ranbp3 A G 17: 57,003,718 (GRCm39) D39G probably damaging Het
Rcor3 T C 1: 191,786,021 (GRCm39) T406A probably benign Het
Rhbdl2 T A 4: 123,718,712 (GRCm39) I222K probably damaging Het
Ripk2 T C 4: 16,151,968 (GRCm39) N197S possibly damaging Het
Rpia A T 6: 70,743,602 (GRCm39) M283K probably damaging Het
Rps6kc1 T C 1: 190,604,022 (GRCm39) D200G probably benign Het
Sipa1l1 A T 12: 82,389,271 (GRCm39) K499M probably damaging Het
Slc5a4b A G 10: 75,906,407 (GRCm39) L404P probably damaging Het
Son AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG 16: 91,457,222 (GRCm39) probably benign Het
Spmip4 G A 6: 50,566,078 (GRCm39) probably null Het
St7 A C 6: 17,854,998 (GRCm39) T312P probably damaging Het
Strbp T C 2: 37,515,294 (GRCm39) E244G probably damaging Het
Tbc1d8 T G 1: 39,416,061 (GRCm39) T871P probably benign Het
Tcp1 T A 17: 13,141,049 (GRCm39) I286N probably damaging Het
Tead3 C A 17: 28,552,491 (GRCm39) V327F probably damaging Het
Tmem132c A T 5: 127,640,504 (GRCm39) T892S probably benign Het
Trmt2a T C 16: 18,068,487 (GRCm39) S65P probably benign Het
Ubp1 A G 9: 113,802,597 (GRCm39) N479S possibly damaging Het
Ubtfl1 A T 9: 18,320,527 (GRCm39) L18F possibly damaging Het
Uevld T G 7: 46,587,804 (GRCm39) M299L probably benign Het
Unc13b T A 4: 43,173,854 (GRCm39) Y1561N unknown Het
Ylpm1 A G 12: 85,075,896 (GRCm39) K874E probably damaging Het
Zbtb37 G A 1: 160,859,802 (GRCm39) R168W possibly damaging Het
Other mutations in Grk4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00509:Grk4 APN 5 34,873,634 (GRCm39) missense probably damaging 0.99
IGL00574:Grk4 APN 5 34,852,162 (GRCm39) missense probably benign 0.00
IGL02127:Grk4 APN 5 34,867,530 (GRCm39) missense probably benign 0.00
IGL02191:Grk4 APN 5 34,912,533 (GRCm39) missense probably benign 0.27
IGL02227:Grk4 APN 5 34,852,126 (GRCm39) missense probably benign 0.06
IGL03152:Grk4 APN 5 34,902,701 (GRCm39) missense probably damaging 1.00
IGL03214:Grk4 APN 5 34,909,553 (GRCm39) missense probably benign
F5426:Grk4 UTSW 5 34,902,503 (GRCm39) splice site probably benign
R0110:Grk4 UTSW 5 34,873,557 (GRCm39) missense probably damaging 0.97
R0469:Grk4 UTSW 5 34,873,557 (GRCm39) missense probably damaging 0.97
R0671:Grk4 UTSW 5 34,905,611 (GRCm39) missense probably benign 0.04
R1466:Grk4 UTSW 5 34,852,094 (GRCm39) missense probably benign 0.02
R1466:Grk4 UTSW 5 34,852,094 (GRCm39) missense probably benign 0.02
R1584:Grk4 UTSW 5 34,852,094 (GRCm39) missense probably benign 0.02
R1605:Grk4 UTSW 5 34,831,901 (GRCm39) missense probably damaging 0.98
R1607:Grk4 UTSW 5 34,888,882 (GRCm39) missense probably benign 0.01
R1903:Grk4 UTSW 5 34,833,531 (GRCm39) splice site probably null
R2352:Grk4 UTSW 5 34,826,520 (GRCm39) missense probably benign 0.04
R4561:Grk4 UTSW 5 34,852,157 (GRCm39) missense probably benign 0.00
R4580:Grk4 UTSW 5 34,818,325 (GRCm39) missense probably damaging 1.00
R4807:Grk4 UTSW 5 34,909,552 (GRCm39) missense probably benign
R5412:Grk4 UTSW 5 34,902,612 (GRCm39) missense probably benign 0.00
R5905:Grk4 UTSW 5 34,869,074 (GRCm39) missense probably damaging 1.00
R6360:Grk4 UTSW 5 34,831,881 (GRCm39) missense probably damaging 1.00
R6604:Grk4 UTSW 5 34,877,208 (GRCm39) missense probably damaging 1.00
R6865:Grk4 UTSW 5 34,888,894 (GRCm39) missense probably damaging 1.00
R7265:Grk4 UTSW 5 34,873,608 (GRCm39) missense probably damaging 0.96
R7394:Grk4 UTSW 5 34,908,962 (GRCm39) missense probably benign
R7821:Grk4 UTSW 5 34,867,553 (GRCm39) missense probably damaging 1.00
R8074:Grk4 UTSW 5 34,833,482 (GRCm39) missense probably benign 0.30
R8218:Grk4 UTSW 5 34,826,540 (GRCm39) missense probably benign 0.01
R8499:Grk4 UTSW 5 34,902,690 (GRCm39) missense possibly damaging 0.90
R9026:Grk4 UTSW 5 34,877,084 (GRCm39) missense probably damaging 1.00
R9068:Grk4 UTSW 5 34,905,653 (GRCm39) missense
X0064:Grk4 UTSW 5 34,877,228 (GRCm39) missense possibly damaging 0.76
Predicted Primers PCR Primer
(F):5'- GGTACTGGGTGATACTTTGACTT -3'
(R):5'- CTTTTGTCTTGGCGGTTACCA -3'

Sequencing Primer
(F):5'- GTAGAGTAGGCATTAGGTTC -3'
(R):5'- TACCATCCTAGAGCCTTAGAAGTAGG -3'
Posted On 2019-11-12