Mutagenetix > Incidental Mutation

Incidental Mutation 'R7722:Tg'

595301

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

045778-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R7722 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66670753-66850721 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 66764309 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 582 (R582C)

Ref Sequence

ENSEMBL: ENSMUSP00000126454 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916] [ENSMUST00000171045]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000065916
AA Change: R2201C

PolyPhen 2 Score 0.728 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469
AA Change: R2201C

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000171045
AA Change: R582C

PolyPhen 2 Score 0.728 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469
AA Change: R582C

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (57/58)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Afdn	A	G	17: 13,808,969 (GRCm38)	E151G	probably benign	Het
Alpk2	A	T	18: 65,350,157 (GRCm38)	L260H	probably damaging	Het
Amh	T	C	10: 80,806,624 (GRCm38)	V247A	probably benign	Het
Ank2	T	A	3: 127,029,302 (GRCm38)	I431L	probably benign	Het
Ano7	G	A	1: 93,390,423 (GRCm38)	A286T	probably damaging	Het
Atp8a1	T	A	5: 67,622,698 (GRCm38)		probably null	Het
Brd1	A	T	15: 88,729,559 (GRCm38)	S378T	probably damaging	Het
Cd163l1	C	A	7: 140,222,386 (GRCm38)	C209*	probably null	Het
Clrn1	T	A	3: 58,846,334 (GRCm38)	N202I	possibly damaging	Het
Cspp1	T	C	1: 10,074,901 (GRCm38)	V308A	probably benign	Het
Cyp2a5	T	C	7: 26,837,118 (GRCm38)	L174P	probably benign	Het
Ehbp1	G	A	11: 22,089,572 (GRCm38)	H843Y	probably null	Het
Fancm	T	A	12: 65,106,461 (GRCm38)	D1230E	probably damaging	Het
Fbxl17	A	T	17: 63,356,828 (GRCm38)	N555K	probably damaging	Het
Fmnl2	A	C	2: 53,054,467 (GRCm38)	S153R		Het
Fras1	G	A	5: 96,769,554 (GRCm38)	V3478M	probably damaging	Het
Gnptab	T	C	10: 88,379,528 (GRCm38)	F37S	probably damaging	Het
Gpr183	T	G	14: 121,954,858 (GRCm38)	I84L	probably damaging	Het
Hcn1	A	G	13: 117,902,778 (GRCm38)	H410R	unknown	Het
Hmcn1	A	G	1: 150,667,880 (GRCm38)	V2848A	probably damaging	Het
Hmcn2	A	T	2: 31,382,500 (GRCm38)	R1331*	probably null	Het
Hsd17b4	A	C	18: 50,146,524 (GRCm38)	N190T	probably damaging	Het
Kif14	A	G	1: 136,468,295 (GRCm38)	D113G	probably benign	Het
Kmt2e	T	G	5: 23,497,018 (GRCm38)	D881E	probably benign	Het
Lamb1	T	A	12: 31,323,571 (GRCm38)	L1481Q	probably damaging	Het
Limk2	T	C	11: 3,356,092 (GRCm38)		probably null	Het
Luc7l2	T	A	6: 38,603,308 (GRCm38)	S281T	unknown	Het
Map3k5	A	G	10: 20,132,145 (GRCm38)	D1240G	probably benign	Het
Med13l	C	A	5: 118,747,407 (GRCm38)	T1475K	probably benign	Het
Med17	G	T	9: 15,271,691 (GRCm38)	Q353K	probably benign	Het
Mlh3	A	G	12: 85,267,492 (GRCm38)	V640A	probably benign	Het
Mms22l	T	A	4: 24,517,201 (GRCm38)	Y361N	probably damaging	Het
Mtmr2	T	A	9: 13,804,808 (GRCm38)	N532K	probably benign	Het
Mtss1	G	A	15: 59,055,086 (GRCm38)	T47I	probably damaging	Het
Mturn	T	C	6: 54,699,560 (GRCm38)		probably null	Het
Muc20	A	C	16: 32,797,386 (GRCm38)	S3A	probably benign	Het
Nde1	T	C	16: 14,190,264 (GRCm38)	Y164H	unknown	Het
Neurl1b	A	G	17: 26,441,158 (GRCm38)	T451A	probably benign	Het
Olfr1537	A	T	9: 39,237,589 (GRCm38)	Y278*	probably null	Het
Olfr654	C	A	7: 104,588,298 (GRCm38)	Q165K	possibly damaging	Het
Pgap3	G	A	11: 98,390,784 (GRCm38)	A196V	probably benign	Het
Rsf1	GGCG	GGCGACGGCCGCG	7: 97,579,906 (GRCm38)		probably benign	Het
Sdcbp	T	A	4: 6,385,063 (GRCm38)	V94E	possibly damaging	Het
Sgo2a	T	G	1: 58,016,537 (GRCm38)	F627V	probably benign	Het
Skor2	A	C	18: 76,862,644 (GRCm38)	N889T	probably benign	Het
Slc35f4	A	T	14: 49,306,274 (GRCm38)	N288K	probably benign	Het
Slc9b2	T	A	3: 135,329,835 (GRCm38)	V355E	probably null	Het
Smarcc2	A	G	10: 128,481,728 (GRCm38)	E566G	possibly damaging	Het
Sorcs2	T	C	5: 36,043,527 (GRCm38)	E559G	probably damaging	Het
Sphkap	T	C	1: 83,278,921 (GRCm38)	D369G	probably benign	Het
Ssfa2	T	C	2: 79,662,345 (GRCm38)	S1079P	probably damaging	Het
Stc1	T	A	14: 69,032,280 (GRCm38)	I103N	possibly damaging	Het
Stx8	T	C	11: 68,203,718 (GRCm38)	V219A	probably damaging	Het
Tshz2	T	A	2: 169,885,272 (GRCm38)	L596Q	probably benign	Het
Tti1	A	G	2: 158,007,607 (GRCm38)	Y571H	probably benign	Het
Zbtb40	A	G	4: 136,991,518 (GRCm38)	I956T	probably damaging	Het
Zfp990	A	C	4: 145,536,962 (GRCm38)	N177H	possibly damaging	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,847,166 (GRCm38)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,827,290 (GRCm38)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,693,424 (GRCm38)	missense	probably benign
IGL00428:Tg	APN	15	66,773,424 (GRCm38)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,696,489 (GRCm38)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,683,813 (GRCm38)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,688,801 (GRCm38)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,674,073 (GRCm38)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,764,453 (GRCm38)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,681,882 (GRCm38)	missense	probably benign
IGL01288:Tg	APN	15	66,736,276 (GRCm38)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,696,092 (GRCm38)	splice site	probably benign
IGL01634:Tg	APN	15	66,729,566 (GRCm38)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,678,087 (GRCm38)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,671,351 (GRCm38)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,759,486 (GRCm38)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,692,374 (GRCm38)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,705,330 (GRCm38)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,717,233 (GRCm38)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,705,348 (GRCm38)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,757,943 (GRCm38)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,764,291 (GRCm38)	missense	probably benign
IGL02434:Tg	APN	15	66,764,342 (GRCm38)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,741,594 (GRCm38)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,705,274 (GRCm38)	missense	probably benign
IGL02549:Tg	APN	15	66,839,361 (GRCm38)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,748,726 (GRCm38)	splice site	probably benign
IGL02756:Tg	APN	15	66,734,586 (GRCm38)	missense	probably benign
IGL02800:Tg	APN	15	66,757,886 (GRCm38)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,682,394 (GRCm38)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,671,405 (GRCm38)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,715,106 (GRCm38)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,839,303 (GRCm38)	nonsense	probably null
IGL03242:Tg	APN	15	66,683,798 (GRCm38)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
foster	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
hognose	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
ito	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
ito2	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
ito3	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
ito4	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
Papua	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
pluribus	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
samarai	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
sariba	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
ticker	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
Vampire	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,740,718 (GRCm38)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,740,781 (GRCm38)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,698,446 (GRCm38)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,764,442 (GRCm38)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,828,533 (GRCm38)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,682,404 (GRCm38)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,729,597 (GRCm38)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,729,626 (GRCm38)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,737,521 (GRCm38)	missense	probably benign
R0673:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,839,404 (GRCm38)	splice site	probably benign
R0704:Tg	UTSW	15	66,757,880 (GRCm38)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,678,789 (GRCm38)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,725,144 (GRCm38)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,708,010 (GRCm38)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,672,409 (GRCm38)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,698,559 (GRCm38)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,684,062 (GRCm38)	missense	probably benign
R1103:Tg	UTSW	15	66,719,655 (GRCm38)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,828,548 (GRCm38)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,696,489 (GRCm38)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,850,502 (GRCm38)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,705,232 (GRCm38)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,693,430 (GRCm38)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,729,685 (GRCm38)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,696,166 (GRCm38)	nonsense	probably null
R1655:Tg	UTSW	15	66,828,568 (GRCm38)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,692,387 (GRCm38)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,737,548 (GRCm38)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,671,309 (GRCm38)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,682,842 (GRCm38)	missense	probably benign
R2063:Tg	UTSW	15	66,828,553 (GRCm38)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,849,607 (GRCm38)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,729,594 (GRCm38)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,681,939 (GRCm38)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,681,933 (GRCm38)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,683,898 (GRCm38)	missense	probably benign
R2994:Tg	UTSW	15	66,681,953 (GRCm38)	missense	probably benign
R3904:Tg	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
R3946:Tg	UTSW	15	66,674,023 (GRCm38)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,684,190 (GRCm38)	missense	probably benign
R4245:Tg	UTSW	15	66,696,469 (GRCm38)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,766,147 (GRCm38)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,707,942 (GRCm38)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,735,271 (GRCm38)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,673,920 (GRCm38)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,693,319 (GRCm38)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,764,337 (GRCm38)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,696,586 (GRCm38)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,707,930 (GRCm38)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,681,813 (GRCm38)	splice site	probably null
R5049:Tg	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
R5073:Tg	UTSW	15	66,735,252 (GRCm38)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,678,780 (GRCm38)	nonsense	probably null
R5185:Tg	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,759,567 (GRCm38)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,678,855 (GRCm38)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,678,055 (GRCm38)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,739,168 (GRCm38)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,827,293 (GRCm38)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,685,300 (GRCm38)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,693,435 (GRCm38)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,838,057 (GRCm38)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,688,889 (GRCm38)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,683,993 (GRCm38)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,828,457 (GRCm38)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,673,367 (GRCm38)	splice site	probably null
R6159:Tg	UTSW	15	66,735,247 (GRCm38)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,707,922 (GRCm38)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,671,311 (GRCm38)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,759,558 (GRCm38)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,735,259 (GRCm38)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,688,891 (GRCm38)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,696,246 (GRCm38)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,693,358 (GRCm38)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,673,543 (GRCm38)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,694,784 (GRCm38)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,725,272 (GRCm38)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,696,583 (GRCm38)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,696,588 (GRCm38)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,696,161 (GRCm38)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,694,768 (GRCm38)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,689,927 (GRCm38)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,764,418 (GRCm38)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,729,583 (GRCm38)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
R7790:Tg	UTSW	15	66,849,604 (GRCm38)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,693,263 (GRCm38)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,683,814 (GRCm38)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,705,279 (GRCm38)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,684,074 (GRCm38)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,683,793 (GRCm38)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,773,398 (GRCm38)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
R8688:Tg	UTSW	15	66,694,953 (GRCm38)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,681,937 (GRCm38)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,684,042 (GRCm38)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,685,335 (GRCm38)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,773,483 (GRCm38)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,683,673 (GRCm38)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,698,461 (GRCm38)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,827,269 (GRCm38)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,685,397 (GRCm38)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,689,324 (GRCm38)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,847,074 (GRCm38)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,674,064 (GRCm38)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,735,260 (GRCm38)	nonsense	probably null
R9629:Tg	UTSW	15	66,683,738 (GRCm38)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,766,142 (GRCm38)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,689,990 (GRCm38)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,847,159 (GRCm38)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,682,454 (GRCm38)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,748,743 (GRCm38)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,849,547 (GRCm38)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,685,310 (GRCm38)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- TCATACTTGGTGCTGTTAACATTGG -3'
(R):5'- GCTTGGTAGCATCCCATGAAC -3'

Sequencing Primer
(F):5'- ACATTGGATGTTAACCTTTGCC -3'
(R):5'- GGTAGCATCCCATGAACCTGTC -3'

Posted On

2019-11-12