Mutagenetix > Incidental Mutation

Incidental Mutation 'R7725:Med12l'

595433

Institutional Source

Beutler Lab

Gene Symbol

Med12l

Ensembl Gene

ENSMUSG00000056476

Gene Name

mediator complex subunit 12-like

Synonyms

MMRRC Submission

045781-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.257) question?

Stock #

R7725 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

58913246-59226103 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 59163413 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 1347 (K1347E)

Ref Sequence

ENSEMBL: ENSMUSP00000127038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040325] [ENSMUST00000050360] [ENSMUST00000164225] [ENSMUST00000199609] [ENSMUST00000199659]

AlphaFold

Q8BQM9

Predicted Effect

probably benign
Transcript: ENSMUST00000040325
AA Change: K1312E

PolyPhen 2 Score 0.159 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000042269
Gene: ENSMUSG00000056476
AA Change: K1312E

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	730	2.6e-207	PFAM
low complexity region	744	758	N/A	INTRINSIC
low complexity region	853	872	N/A	INTRINSIC
low complexity region	1455	1466	N/A	INTRINSIC
low complexity region	1728	1742	N/A	INTRINSIC
low complexity region	1769	1783	N/A	INTRINSIC
Pfam:Med12-PQL	1803	2029	2.3e-14	PFAM
low complexity region	2055	2076	N/A	INTRINSIC
low complexity region	2083	2101	N/A	INTRINSIC
low complexity region	2116	2136	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000050360

SMART Domains

Protein: ENSMUSP00000051353
Gene: ENSMUSG00000036353

Domain	Start	End	E-Value	Type
Pfam:7tm_1	48	304	1.3e-40	PFAM
low complexity region	322	335	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164225
AA Change: K1347E

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000127038
Gene: ENSMUSG00000056476
AA Change: K1347E

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	283	765	5e-187	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1763	1777	N/A	INTRINSIC
low complexity region	1804	1818	N/A	INTRINSIC
Pfam:Med12-PQL	1840	2063	9.7e-66	PFAM
low complexity region	2090	2111	N/A	INTRINSIC
low complexity region	2118	2136	N/A	INTRINSIC
low complexity region	2151	2171	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000197374

Predicted Effect

probably benign
Transcript: ENSMUST00000199609

SMART Domains

Protein: ENSMUSP00000143521
Gene: ENSMUSG00000036353

Domain	Start	End	E-Value	Type
Pfam:7tm_1	23	304	1.5e-31	PFAM
low complexity region	322	335	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000199659
AA Change: K1347E

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000142903
Gene: ENSMUSG00000056476
AA Change: K1347E

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	765	5.5e-209	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1761	1775	N/A	INTRINSIC
low complexity region	1802	1816	N/A	INTRINSIC
Pfam:Med12-PQL	1836	2062	1.7e-15	PFAM
low complexity region	2088	2130	N/A	INTRINSIC
low complexity region	2144	2164	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.3%

Validation Efficiency

100% (41/41)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010]

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Anxa2	A	T	9: 69,387,410 (GRCm39)	K6N	unknown	Het
Arhgap20	T	A	9: 51,743,050 (GRCm39)	M296K	possibly damaging	Het
Bdh1	G	A	16: 31,256,910 (GRCm39)	V20I	not run	Het
Bop1	A	G	15: 76,339,583 (GRCm39)	I254T	probably benign	Het
C1ra	G	A	6: 124,494,684 (GRCm39)	E316K	probably benign	Het
Cct4	A	G	11: 22,940,814 (GRCm39)	K21E	probably benign	Het
Cercam	G	A	2: 29,762,574 (GRCm39)		probably null	Het
Clip2	A	T	5: 134,546,853 (GRCm39)	Y238*	probably null	Het
Col19a1	A	T	1: 24,309,525 (GRCm39)	S1043T	possibly damaging	Het
Ctcf	A	G	8: 106,390,468 (GRCm39)	Y25C	probably damaging	Het
Dennd4c	C	T	4: 86,704,330 (GRCm39)	R282C	probably benign	Het
Eef1g	T	A	19: 8,955,427 (GRCm39)	H425Q	probably benign	Het
Fam131b	C	T	6: 42,295,476 (GRCm39)	A234T	probably benign	Het
Fam184a	C	A	10: 53,509,802 (GRCm39)	E126*	probably null	Het
Fam187b	A	G	7: 30,677,139 (GRCm39)	D216G	possibly damaging	Het
Gabra1	A	T	11: 42,026,270 (GRCm39)	Y341N	possibly damaging	Het
Glb1l3	T	A	9: 26,739,659 (GRCm39)		probably null	Het
Gm3667	T	A	14: 18,271,599 (GRCm39)	Q52L	probably damaging	Het
Gpr179	C	T	11: 97,242,118 (GRCm39)	R242H	probably damaging	Het
Gulo	T	C	14: 66,245,522 (GRCm39)	Y24C	probably damaging	Het
Hectd4	A	T	5: 121,358,680 (GRCm39)	E49V	unknown	Het
Htra4	A	G	8: 25,527,169 (GRCm39)	S209P	possibly damaging	Het
Lmntd1	A	G	6: 145,489,196 (GRCm39)	S22P	probably benign	Het
Lypd8l	A	T	11: 58,499,325 (GRCm39)	N164K	probably benign	Het
Mrc1	C	A	2: 14,284,788 (GRCm39)	D592E	probably benign	Het
Muc15	A	G	2: 110,562,143 (GRCm39)	D193G	probably damaging	Het
Ncor2	A	G	5: 125,100,630 (GRCm39)	V1316A		Het
Odad1	T	A	7: 45,597,835 (GRCm39)	S582T	probably damaging	Het
Or2t43	A	G	11: 58,457,593 (GRCm39)	Y193H	probably damaging	Het
Or2y10	A	T	11: 49,455,359 (GRCm39)	I204L	probably benign	Het
Or5t16	T	C	2: 86,819,323 (GRCm39)	I66V	probably benign	Het
Serpina6	A	T	12: 103,614,936 (GRCm39)	Y303*	probably null	Het
Shld2	T	G	14: 33,990,661 (GRCm39)	T82P	possibly damaging	Het
Shroom3	T	C	5: 93,089,512 (GRCm39)	L754P	probably benign	Het
Skint5	C	A	4: 113,685,099 (GRCm39)	L539F	unknown	Het
St3gal4	C	A	9: 34,964,375 (GRCm39)	R209L	possibly damaging	Het
Tex14	A	C	11: 87,385,868 (GRCm39)	T243P	probably damaging	Het
Ttc12	T	A	9: 49,351,602 (GRCm39)	M632L	probably benign	Het
Ttn	T	C	2: 76,738,116 (GRCm39)	E4187G	unknown	Het
Txk	G	A	5: 72,864,900 (GRCm39)	A379V	probably damaging	Het
Wdr43	A	G	17: 71,923,338 (GRCm39)	Y28C	probably benign	Het
Zfp1005	A	G	2: 150,110,468 (GRCm39)	Y386C	unknown	Het
Zfp40	A	G	17: 23,397,251 (GRCm39)	V82A	probably benign	Het
Zfp994	A	T	17: 22,419,091 (GRCm39)	N619K	probably benign	Het

Other mutations in Med12l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Med12l	APN	3	58,949,757 (GRCm39)	missense	probably damaging	0.98
IGL00561:Med12l	APN	3	59,135,245 (GRCm39)	missense	probably benign
IGL00974:Med12l	APN	3	58,990,435 (GRCm39)	missense	probably damaging	1.00
IGL01024:Med12l	APN	3	58,980,762 (GRCm39)	missense	probably damaging	1.00
IGL01094:Med12l	APN	3	59,001,076 (GRCm39)	missense	probably damaging	0.99
IGL01134:Med12l	APN	3	58,949,696 (GRCm39)	missense	possibly damaging	0.91
IGL01535:Med12l	APN	3	59,169,680 (GRCm39)	missense	probably damaging	1.00
IGL01653:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL01735:Med12l	APN	3	59,170,675 (GRCm39)	missense	probably damaging	1.00
IGL01972:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL02005:Med12l	APN	3	59,152,368 (GRCm39)	missense	probably damaging	1.00
IGL02098:Med12l	APN	3	59,183,276 (GRCm39)	missense	possibly damaging	0.92
IGL02115:Med12l	APN	3	58,975,740 (GRCm39)	missense	probably benign	0.00
IGL02231:Med12l	APN	3	59,153,303 (GRCm39)	missense	probably damaging	1.00
IGL02259:Med12l	APN	3	59,153,264 (GRCm39)	missense	probably damaging	1.00
IGL02369:Med12l	APN	3	59,164,794 (GRCm39)	missense	probably benign	0.00
IGL02424:Med12l	APN	3	59,000,143 (GRCm39)	missense	probably benign	0.21
IGL02501:Med12l	APN	3	59,169,397 (GRCm39)	missense	possibly damaging	0.71
IGL02525:Med12l	APN	3	58,975,789 (GRCm39)	missense	probably benign	0.01
IGL02530:Med12l	APN	3	58,984,510 (GRCm39)	missense	probably damaging	1.00
IGL02735:Med12l	APN	3	59,001,067 (GRCm39)	missense	probably damaging	1.00
IGL02865:Med12l	APN	3	59,201,713 (GRCm39)	missense	probably damaging	1.00
IGL03183:Med12l	APN	3	58,944,976 (GRCm39)	splice site	probably null
IGL03264:Med12l	APN	3	59,208,788 (GRCm39)	nonsense	probably null
FR4304:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4340:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,415 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,409 (GRCm39)	small insertion	probably benign
FR4449:Med12l	UTSW	3	59,183,384 (GRCm39)	nonsense	probably null
FR4548:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4589:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
FR4976:Med12l	UTSW	3	59,183,398 (GRCm39)	small insertion	probably benign
P0007:Med12l	UTSW	3	58,998,816 (GRCm39)	splice site	probably benign
P0045:Med12l	UTSW	3	58,998,956 (GRCm39)	missense	probably damaging	0.99
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0148:Med12l	UTSW	3	58,945,075 (GRCm39)	missense	probably damaging	1.00
R0325:Med12l	UTSW	3	58,984,480 (GRCm39)	missense	possibly damaging	0.88
R0330:Med12l	UTSW	3	59,135,123 (GRCm39)	missense	probably damaging	1.00
R0388:Med12l	UTSW	3	59,000,925 (GRCm39)	splice site	probably benign
R0542:Med12l	UTSW	3	58,949,822 (GRCm39)	missense	probably damaging	1.00
R0624:Med12l	UTSW	3	58,945,123 (GRCm39)	nonsense	probably null
R0625:Med12l	UTSW	3	59,154,858 (GRCm39)	missense	probably damaging	1.00
R0671:Med12l	UTSW	3	59,172,350 (GRCm39)	missense	probably damaging	1.00
R0706:Med12l	UTSW	3	59,169,401 (GRCm39)	missense	probably damaging	1.00
R0785:Med12l	UTSW	3	59,168,253 (GRCm39)	missense	probably damaging	1.00
R1054:Med12l	UTSW	3	59,156,072 (GRCm39)	missense	probably damaging	0.99
R1102:Med12l	UTSW	3	59,152,257 (GRCm39)	missense	probably damaging	0.99
R1391:Med12l	UTSW	3	58,945,159 (GRCm39)	missense	probably benign	0.00
R1501:Med12l	UTSW	3	59,168,256 (GRCm39)	critical splice donor site	probably null
R1544:Med12l	UTSW	3	59,172,661 (GRCm39)	missense	possibly damaging	0.71
R1662:Med12l	UTSW	3	59,001,038 (GRCm39)	missense	probably damaging	1.00
R1670:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
R1839:Med12l	UTSW	3	58,975,740 (GRCm39)	missense	probably benign
R1854:Med12l	UTSW	3	59,168,193 (GRCm39)	missense	probably damaging	1.00
R2045:Med12l	UTSW	3	59,169,731 (GRCm39)	nonsense	probably null
R2070:Med12l	UTSW	3	59,152,326 (GRCm39)	missense	probably damaging	1.00
R2132:Med12l	UTSW	3	59,172,703 (GRCm39)	splice site	probably null
R2290:Med12l	UTSW	3	59,152,359 (GRCm39)	missense	probably damaging	1.00
R2325:Med12l	UTSW	3	59,139,875 (GRCm39)	missense	probably damaging	0.99
R2352:Med12l	UTSW	3	59,148,113 (GRCm39)	missense	probably damaging	1.00
R2484:Med12l	UTSW	3	59,205,259 (GRCm39)	missense	probably benign	0.18
R2906:Med12l	UTSW	3	59,164,503 (GRCm39)	missense	probably damaging	1.00
R3735:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3736:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3774:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R3957:Med12l	UTSW	3	58,980,589 (GRCm39)	missense	probably damaging	0.99
R4020:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R4087:Med12l	UTSW	3	59,205,342 (GRCm39)	missense	probably benign	0.00
R4231:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4233:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4235:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4236:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4327:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4328:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4346:Med12l	UTSW	3	58,938,976 (GRCm39)	missense	probably damaging	1.00
R4543:Med12l	UTSW	3	58,998,929 (GRCm39)	missense	probably damaging	1.00
R4559:Med12l	UTSW	3	58,914,523 (GRCm39)	critical splice donor site	probably null
R4776:Med12l	UTSW	3	59,140,633 (GRCm39)	missense	probably damaging	1.00
R4877:Med12l	UTSW	3	59,152,214 (GRCm39)	missense	probably damaging	1.00
R4983:Med12l	UTSW	3	59,169,350 (GRCm39)	missense	probably damaging	1.00
R5114:Med12l	UTSW	3	59,167,109 (GRCm39)	missense	possibly damaging	0.85
R5125:Med12l	UTSW	3	59,174,635 (GRCm39)	missense	possibly damaging	0.83
R5230:Med12l	UTSW	3	59,153,209 (GRCm39)	missense	probably damaging	1.00
R5407:Med12l	UTSW	3	59,165,622 (GRCm39)	missense	probably damaging	1.00
R5426:Med12l	UTSW	3	59,156,143 (GRCm39)	missense	probably damaging	0.98
R5439:Med12l	UTSW	3	59,170,634 (GRCm39)	missense	probably null	1.00
R5449:Med12l	UTSW	3	59,167,127 (GRCm39)	missense	probably damaging	1.00
R5596:Med12l	UTSW	3	59,159,771 (GRCm39)	missense	probably benign	0.45
R5716:Med12l	UTSW	3	59,208,798 (GRCm39)	critical splice donor site	probably null
R5833:Med12l	UTSW	3	59,172,647 (GRCm39)	missense	possibly damaging	0.95
R5883:Med12l	UTSW	3	58,998,889 (GRCm39)	missense	probably damaging	1.00
R6264:Med12l	UTSW	3	59,163,423 (GRCm39)	missense	probably damaging	1.00
R6269:Med12l	UTSW	3	59,135,243 (GRCm39)	missense	probably damaging	1.00
R6394:Med12l	UTSW	3	59,142,508 (GRCm39)	missense	probably damaging	1.00
R6400:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R6475:Med12l	UTSW	3	59,164,500 (GRCm39)	missense	probably damaging	1.00
R6489:Med12l	UTSW	3	59,164,828 (GRCm39)	missense	probably damaging	0.99
R6654:Med12l	UTSW	3	59,169,713 (GRCm39)	missense	probably damaging	1.00
R6881:Med12l	UTSW	3	59,174,586 (GRCm39)	missense	probably benign	0.00
R7110:Med12l	UTSW	3	59,169,645 (GRCm39)	missense	possibly damaging	0.92
R7134:Med12l	UTSW	3	59,001,180 (GRCm39)	nonsense	probably null
R7137:Med12l	UTSW	3	59,165,675 (GRCm39)	missense	probably damaging	1.00
R7159:Med12l	UTSW	3	59,183,438 (GRCm39)	missense	probably benign
R7341:Med12l	UTSW	3	58,949,824 (GRCm39)	missense	possibly damaging	0.53
R7349:Med12l	UTSW	3	59,165,746 (GRCm39)	missense	probably damaging	1.00
R7413:Med12l	UTSW	3	58,998,971 (GRCm39)	missense	probably benign	0.00
R7495:Med12l	UTSW	3	59,152,194 (GRCm39)	missense	probably damaging	1.00
R7678:Med12l	UTSW	3	58,984,141 (GRCm39)	missense	probably damaging	1.00
R7697:Med12l	UTSW	3	59,148,078 (GRCm39)	missense	probably damaging	1.00
R7714:Med12l	UTSW	3	59,001,007 (GRCm39)	missense	probably benign	0.17
R7846:Med12l	UTSW	3	59,172,355 (GRCm39)	missense	probably damaging	1.00
R7852:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R8080:Med12l	UTSW	3	59,172,607 (GRCm39)	missense	probably damaging	1.00
R8181:Med12l	UTSW	3	59,169,389 (GRCm39)	missense	probably damaging	1.00
R8223:Med12l	UTSW	3	58,993,784 (GRCm39)	missense	possibly damaging	0.79
R8560:Med12l	UTSW	3	58,945,026 (GRCm39)	missense	probably damaging	1.00
R8708:Med12l	UTSW	3	59,159,751 (GRCm39)	missense	probably benign	0.00
R8865:Med12l	UTSW	3	58,979,303 (GRCm39)	missense	probably benign
R8947:Med12l	UTSW	3	58,984,443 (GRCm39)	splice site	probably benign
R8976:Med12l	UTSW	3	59,183,329 (GRCm39)	missense	probably damaging	0.99
R9016:Med12l	UTSW	3	59,163,294 (GRCm39)	missense	probably damaging	0.96
R9183:Med12l	UTSW	3	58,984,498 (GRCm39)	missense	probably damaging	1.00
R9487:Med12l	UTSW	3	59,155,353 (GRCm39)	missense	probably benign
R9526:Med12l	UTSW	3	58,984,207 (GRCm39)	missense	probably damaging	0.96
R9802:Med12l	UTSW	3	59,169,346 (GRCm39)	missense	probably damaging	1.00
RF004:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF011:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF013:Med12l	UTSW	3	59,183,387 (GRCm39)	small insertion	probably benign
RF020:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
RF021:Med12l	UTSW	3	58,980,711 (GRCm39)	missense	probably benign	0.19
RF027:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF027:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF030:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,408 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF037:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF049:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF050:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF053:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF055:Med12l	UTSW	3	59,183,404 (GRCm39)	small insertion	probably benign
RF056:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF057:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
X0062:Med12l	UTSW	3	59,140,600 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	59,203,538 (GRCm39)	missense	probably benign	0.00
Z1176:Med12l	UTSW	3	59,152,364 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	58,998,838 (GRCm39)	missense	probably damaging	0.98
Z1177:Med12l	UTSW	3	59,155,296 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGGTGGGAGAACACTGCT -3'
(R):5'- GGCAATGTCACTGTAAAGAAAATTAC -3'

Sequencing Primer
(F):5'- TTAAAAGAACCAGAACGACTGTGTAC -3'
(R):5'- CTAGTCGGCCATCACTGGAAAG -3'

Posted On

2019-11-12