Incidental Mutation 'R7730:Zfp964'
ID595766
Institutional Source Beutler Lab
Gene Symbol Zfp964
Ensembl Gene ENSMUSG00000091764
Gene Namezinc finger protein 964
SynonymsGm7187
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.073) question?
Stock #R7730 (G1)
Quality Score225.009
Status Not validated
Chromosome8
Chromosomal Location69654479-69666982 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 69663710 bp
ZygosityHeterozygous
Amino Acid Change Glutamic Acid to Glycine at position 320 (E320G)
Ref Sequence ENSEMBL: ENSMUSP00000145354 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000169125] [ENSMUST00000204285]
Predicted Effect possibly damaging
Transcript: ENSMUST00000169125
AA Change: E319G

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000129822
Gene: ENSMUSG00000091764
AA Change: E319G

DomainStartEndE-ValueType
KRAB 3 56 5.24e-18 SMART
ZnF_C2H2 216 238 4.54e-4 SMART
ZnF_C2H2 244 266 3.58e-2 SMART
ZnF_C2H2 272 294 2.2e-2 SMART
ZnF_C2H2 300 322 5.5e-3 SMART
ZnF_C2H2 328 350 8.22e-2 SMART
ZnF_C2H2 356 378 2.05e-2 SMART
ZnF_C2H2 384 406 6.32e-3 SMART
ZnF_C2H2 412 434 5.42e-2 SMART
ZnF_C2H2 440 462 1.28e-3 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000204285
AA Change: E320G

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000145354
Gene: ENSMUSG00000091764
AA Change: E320G

DomainStartEndE-ValueType
KRAB 4 57 5.24e-18 SMART
ZnF_C2H2 217 239 4.54e-4 SMART
ZnF_C2H2 245 267 3.58e-2 SMART
ZnF_C2H2 273 295 2.2e-2 SMART
ZnF_C2H2 301 323 5.5e-3 SMART
ZnF_C2H2 329 351 8.22e-2 SMART
ZnF_C2H2 357 379 2.05e-2 SMART
ZnF_C2H2 385 407 6.32e-3 SMART
ZnF_C2H2 413 435 5.42e-2 SMART
ZnF_C2H2 441 463 1.28e-3 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9930111J21Rik1 T A 11: 48,947,876 H628L probably benign Het
Adam6a T A 12: 113,544,040 V11E possibly damaging Het
Amotl1 T G 9: 14,555,763 K660T possibly damaging Het
Ap4m1 T C 5: 138,172,815 I59T probably damaging Het
Brd3 T C 2: 27,456,955 Y389C probably damaging Het
C1ra G A 6: 124,517,725 E316K probably benign Het
Card11 C A 5: 140,885,996 R650L probably damaging Het
Cercam G A 2: 29,872,562 probably null Het
Cnst T C 1: 179,625,085 C673R probably damaging Het
Dld G T 12: 31,340,865 T194K probably benign Het
Dnah12 T A 14: 26,785,933 W1714R probably damaging Het
Dsg1a T G 18: 20,331,711 V421G possibly damaging Het
Fer1l4 G T 2: 156,048,934 P188Q probably benign Het
Gpr158 T C 2: 21,826,347 S753P probably damaging Het
Hdc A T 2: 126,594,082 M623K possibly damaging Het
Herc1 CAACCCTGGTAAC CAAC 9: 66,493,190 probably benign Het
Igf2r A T 17: 12,735,991 F203I probably damaging Het
Jag2 A T 12: 112,922,041 I145N probably damaging Het
Kcnt2 T A 1: 140,518,948 F694I probably benign Het
Lpl A T 8: 68,887,448 R32* probably null Het
Mcpt4 T A 14: 56,059,971 I243L probably benign Het
Mtf1 C A 4: 124,838,619 A490E possibly damaging Het
Mycbp2 A T 14: 103,123,355 M4497K probably damaging Het
Myog T C 1: 134,291,176 probably null Het
Nav2 T C 7: 49,572,397 S1757P probably damaging Het
Olfr830 T G 9: 18,875,413 F26V probably benign Het
Osmr T A 15: 6,824,482 I583F probably damaging Het
Phf19 T A 2: 34,895,804 E551V probably damaging Het
Plxnb2 A G 15: 89,162,330 M870T probably benign Het
Psat1 A G 19: 15,918,356 F83L probably damaging Het
Reep1 T A 6: 71,780,741 V108D possibly damaging Het
Rorc A G 3: 94,393,114 T455A probably benign Het
Serinc5 T G 13: 92,685,190 I169S probably damaging Het
Serpinb6c T C 13: 33,899,309 M41V probably damaging Het
Sgsm3 A T 15: 81,008,726 N335Y probably damaging Het
Slamf7 C T 1: 171,641,021 R101H possibly damaging Het
Slc17a4 A T 13: 23,900,520 L427* probably null Het
Slc35a5 T C 16: 45,143,883 Q329R probably damaging Het
Slc45a1 C T 4: 150,630,940 C656Y probably damaging Het
Srsf6 T C 2: 162,931,723 I18T probably damaging Het
Syn3 T G 10: 86,448,909 H109P probably benign Het
Synj2 T C 17: 6,016,287 V580A probably benign Het
Tbc1d9b T C 11: 50,135,915 V70A possibly damaging Het
Tc2n A G 12: 101,651,147 Y402H probably damaging Het
Tmbim4 T C 10: 120,223,862 C164R possibly damaging Het
Tnfrsf11b T A 15: 54,254,074 R262* probably null Het
Tnip1 T C 11: 54,937,979 K121E probably benign Het
Tut1 T C 19: 8,964,376 probably null Het
Uhrf2 T A 19: 30,075,101 C332S probably damaging Het
Vmn2r101 A G 17: 19,611,688 I649V possibly damaging Het
Vwa8 A G 14: 78,995,149 T644A probably benign Het
Zfhx2 T C 14: 55,066,900 H1209R possibly damaging Het
Zfp384 A G 6: 125,031,672 I306V probably benign Het
Zmym2 A T 14: 56,956,181 Y1151F possibly damaging Het
Other mutations in Zfp964
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00580:Zfp964 APN 8 69659393 splice site probably null
R0506:Zfp964 UTSW 8 69663937 missense unknown
R0740:Zfp964 UTSW 8 69663178 missense probably damaging 0.98
R0786:Zfp964 UTSW 8 69664081 missense possibly damaging 0.71
R1158:Zfp964 UTSW 8 69663853 missense unknown
R1204:Zfp964 UTSW 8 69664018 missense probably benign 0.08
R1413:Zfp964 UTSW 8 69663070 missense unknown
R1562:Zfp964 UTSW 8 69663004 missense probably benign
R1663:Zfp964 UTSW 8 69664083 synonymous probably null
R1693:Zfp964 UTSW 8 69664150 missense possibly damaging 0.55
R2029:Zfp964 UTSW 8 69663917 missense unknown
R2847:Zfp964 UTSW 8 69663854 missense unknown
R2849:Zfp964 UTSW 8 69663854 missense unknown
R4111:Zfp964 UTSW 8 69664104 missense probably benign 0.18
R4792:Zfp964 UTSW 8 69664015 missense probably benign 0.18
R4907:Zfp964 UTSW 8 69663322 missense possibly damaging 0.86
R4938:Zfp964 UTSW 8 69664108 missense possibly damaging 0.64
R5688:Zfp964 UTSW 8 69664116 missense probably benign 0.03
R5905:Zfp964 UTSW 8 69663913 missense unknown
R6009:Zfp964 UTSW 8 69663456 missense possibly damaging 0.71
R6021:Zfp964 UTSW 8 69663092 missense unknown
R6028:Zfp964 UTSW 8 69663913 missense unknown
R6374:Zfp964 UTSW 8 69659344 missense possibly damaging 0.93
R6583:Zfp964 UTSW 8 69662983 missense probably damaging 0.98
Predicted Primers PCR Primer
(F):5'- GCGGTACATGAGAACTCACGT -3'
(R):5'- TTCTGGGGCATGTAGACATTTG -3'

Sequencing Primer
(F):5'- TGCGGGAAAGCCTTTCGATAC -3'
(R):5'- CATGTCTCTGAAGGTGACCATGAC -3'
Posted On2019-11-12