Incidental Mutation 'R7736:Nos2'
ID 596276
Institutional Source Beutler Lab
Gene Symbol Nos2
Ensembl Gene ENSMUSG00000020826
Gene Name nitric oxide synthase 2, inducible
Synonyms Nos2a, NOS-II, Nos-2, iNOS
MMRRC Submission 045792-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R7736 (G1)
Quality Score 225.009
Status Not validated
Chromosome 11
Chromosomal Location 78811613-78851052 bp(+) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) T to A at 78813192 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Cysteine to Stop codon at position 33 (C33*)
Ref Sequence ENSEMBL: ENSMUSP00000018610 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000018610] [ENSMUST00000214397]
AlphaFold P29477
PDB Structure MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) (N-[(1,3-BENZODIOXOL-5-YL)METHYL]-1-[2-(1H-IMIDAZOL-1-YL)PYRIMIDIN-4-YL]-4-(METHOXYCARBONYL)-PIPERAZINE-2-ACETAMIDE COMPLEX [X-RAY DIFFRACTION]
MURINE INOSOXY DIMER WITH ISOTHIOUREA BOUND IN THE ACTIVE SITE [X-RAY DIFFRACTION]
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-HYDROXYARGININE AND 4-AMINO TETRAHYDROBIOPTERIN [X-RAY DIFFRACTION]
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-HYDROXYARGININE AND DIHYDROBIOPTERIN [X-RAY DIFFRACTION]
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-HYDROXYARGININE AND TETRAHYDROBIOPTERIN [X-RAY DIFFRACTION]
Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457F Mutation at Tetrahydrobiopterin Binding Site [X-RAY DIFFRACTION]
Murine Inducible Nitric Oxide Synthase Oxygenase Dimer (Delta 65) with W457A Mutation at Tetrahydrobiopterin Binding Site [X-RAY DIFFRACTION]
inducible nitric oxide synthase with Chlorzoxazone bound [X-RAY DIFFRACTION]
inducible nitric oxide synthase with 7-nitroindazole bound [X-RAY DIFFRACTION]
inducible nitric oxide synthase with 6-nitroindazole bound [X-RAY DIFFRACTION]
>> 40 additional structures at PDB <<
Predicted Effect probably null
Transcript: ENSMUST00000018610
AA Change: C33*
SMART Domains Protein: ENSMUSP00000018610
Gene: ENSMUSG00000020826
AA Change: C33*

DomainStartEndE-ValueType
Pfam:NO_synthase 129 491 6.7e-189 PFAM
Pfam:Flavodoxin_1 535 666 5.5e-43 PFAM
Pfam:FAD_binding_1 719 941 8.8e-79 PFAM
Pfam:NAD_binding_1 973 1087 4.1e-24 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000214397
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.2%
Validation Efficiency
MGI Phenotype FUNCTION: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygous deletion of this gene alters susceptibility to infection, response to injury, sepsis and sensory stimuli, cardiac function, osteoclast, platelet and mammary gland physiology, tumor growth, testis and uterus morphology, diet-induced atherosclerosis, and blood, urine and glucose metabolism. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca12 T G 1: 71,359,123 (GRCm39) D561A probably benign Het
Adgrg1 T C 8: 95,731,965 (GRCm39) F204S probably benign Het
Apoa2 T C 1: 171,053,741 (GRCm39) L72P probably damaging Het
Arhgef10 A T 8: 15,030,583 (GRCm39) K987* probably null Het
Arrb1 A G 7: 99,188,981 (GRCm39) D9G unknown Het
Asph A G 4: 9,621,930 (GRCm39) S192P possibly damaging Het
Bcas1 G A 2: 170,229,084 (GRCm39) T309M possibly damaging Het
Bcat2 C T 7: 45,234,617 (GRCm39) T166M possibly damaging Het
Bmp5 A T 9: 75,801,072 (GRCm39) I401L probably damaging Het
Bpifb9b G A 2: 154,154,025 (GRCm39) G261R probably benign Het
Cd53 T A 3: 106,675,252 (GRCm39) Y106F probably benign Het
Cdhr3 T A 12: 33,103,519 (GRCm39) D366V probably benign Het
Ceacam10 G C 7: 24,480,636 (GRCm39) V256L unknown Het
Cilp2 A G 8: 70,334,071 (GRCm39) Y976H probably damaging Het
Cklf A G 8: 104,988,187 (GRCm39) T107A possibly damaging Het
Dhx16 T A 17: 36,192,568 (GRCm39) W167R possibly damaging Het
Dkk2 T G 3: 131,883,775 (GRCm39) L225R probably damaging Het
Dmbt1 A T 7: 130,718,625 (GRCm39) D1782V unknown Het
Ebag9 A T 15: 44,491,800 (GRCm39) D64V probably damaging Het
Eif3j2 T C 18: 43,610,382 (GRCm39) N144D possibly damaging Het
Elp1 T C 4: 56,776,920 (GRCm39) T626A possibly damaging Het
Foxk2 A T 11: 121,190,473 (GRCm39) Q538L possibly damaging Het
Fpgt T G 3: 154,792,747 (GRCm39) I427L probably benign Het
Ganc A T 2: 120,264,295 (GRCm39) N416I possibly damaging Het
Gata6 A G 18: 11,084,379 (GRCm39) Y556C probably damaging Het
Gga2 A T 7: 121,589,747 (GRCm39) V534E probably damaging Het
Gm7324 T A 14: 43,952,256 (GRCm39) S300T possibly damaging Het
Gprc6a A C 10: 51,491,549 (GRCm39) N733K possibly damaging Het
Hivep3 C T 4: 119,952,740 (GRCm39) T352I possibly damaging Het
Ift88 T G 14: 57,683,121 (GRCm39) V266G probably benign Het
Ip6k1 G T 9: 107,922,891 (GRCm39) G341V probably damaging Het
Itga3 G T 11: 94,967,029 (GRCm39) A45E probably damaging Het
Kctd14 T A 7: 97,107,147 (GRCm39) L134Q probably damaging Het
Lats1 C A 10: 7,578,128 (GRCm39) N417K probably damaging Het
Lrrc37a T C 11: 103,388,285 (GRCm39) H2380R unknown Het
Lrrc4c A G 2: 97,460,705 (GRCm39) T444A probably benign Het
M1ap T C 6: 82,982,565 (GRCm39) I283T probably benign Het
Mapre2 T C 18: 24,011,012 (GRCm39) S207P probably benign Het
Moxd1 T C 10: 24,158,608 (GRCm39) F421L probably damaging Het
Or14j4 A T 17: 37,921,303 (GRCm39) L113H probably damaging Het
Or1j16 A T 2: 36,530,197 (GRCm39) I49F probably damaging Het
Or5g26 T C 2: 85,494,758 (GRCm39) T7A probably damaging Het
Otud4 T C 8: 80,382,394 (GRCm39) I201T possibly damaging Het
Pank4 T C 4: 155,054,204 (GRCm39) Y128H probably benign Het
Phf11 C T 14: 59,488,594 (GRCm39) D68N probably benign Het
Pitpnm2 A G 5: 124,261,093 (GRCm39) V1027A possibly damaging Het
Plcb3 A G 19: 6,946,991 (GRCm39) V8A probably benign Het
Por A G 5: 135,759,976 (GRCm39) E221G probably damaging Het
Prokr2 A T 2: 132,223,500 (GRCm39) L14* probably null Het
Ptgis A G 2: 167,033,891 (GRCm39) F459S unknown Het
Ptpru T C 4: 131,515,693 (GRCm39) E887G probably damaging Het
Qrich2 GCTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTG GCTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTG 11: 116,348,367 (GRCm39) probably benign Het
Slc18a1 A G 8: 69,518,206 (GRCm39) probably null Het
Slc27a3 A G 3: 90,296,740 (GRCm39) S120P probably benign Het
Slc2a12 T A 10: 22,540,717 (GRCm39) Y191N probably damaging Het
Snx29 T A 16: 11,185,588 (GRCm39) M57K probably benign Het
Syncrip A G 9: 88,343,721 (GRCm39) probably null Het
Taar4 T A 10: 23,836,897 (GRCm39) V169E probably damaging Het
Tas1r1 C T 4: 152,116,923 (GRCm39) G237D probably benign Het
Tle1 T C 4: 72,117,571 (GRCm39) K30E probably damaging Het
Tmem131l A T 3: 83,847,875 (GRCm39) L330Q probably damaging Het
Tmem67 A G 4: 12,053,455 (GRCm39) F698L probably benign Het
Ttn T A 2: 76,739,574 (GRCm39) Q3701L unknown Het
Vmn2r17 G A 5: 109,600,757 (GRCm39) R685K probably benign Het
Ylpm1 C A 12: 85,059,757 (GRCm39) A321E unknown Het
Zdbf2 T A 1: 63,347,166 (GRCm39) Y1848* probably null Het
Zfand2b T A 1: 75,146,176 (GRCm39) N61K probably null Het
Zfp867 C T 11: 59,354,016 (GRCm39) A438T probably damaging Het
Zkscan14 G A 5: 145,132,319 (GRCm39) T404I probably benign Het
Zrsr2-ps1 C T 11: 22,923,510 (GRCm39) Q95* probably null Het
Other mutations in Nos2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01388:Nos2 APN 11 78,848,278 (GRCm39) missense probably damaging 0.96
IGL01503:Nos2 APN 11 78,836,689 (GRCm39) splice site probably benign
IGL01789:Nos2 APN 11 78,835,483 (GRCm39) splice site probably benign
IGL02797:Nos2 APN 11 78,831,170 (GRCm39) missense probably damaging 1.00
IGL02968:Nos2 APN 11 78,828,463 (GRCm39) missense probably damaging 1.00
R6762_Nos2_754 UTSW 11 78,850,574 (GRCm39) missense possibly damaging 0.90
R0035:Nos2 UTSW 11 78,836,553 (GRCm39) missense probably damaging 1.00
R0265:Nos2 UTSW 11 78,828,428 (GRCm39) missense probably damaging 0.98
R0441:Nos2 UTSW 11 78,819,409 (GRCm39) missense probably benign 0.10
R0504:Nos2 UTSW 11 78,830,903 (GRCm39) missense probably damaging 1.00
R0570:Nos2 UTSW 11 78,826,187 (GRCm39) missense possibly damaging 0.49
R1356:Nos2 UTSW 11 78,843,629 (GRCm39) missense probably benign 0.00
R1538:Nos2 UTSW 11 78,847,396 (GRCm39) missense probably benign 0.00
R3414:Nos2 UTSW 11 78,848,414 (GRCm39) missense probably benign 0.14
R3418:Nos2 UTSW 11 78,850,521 (GRCm39) missense possibly damaging 0.47
R4279:Nos2 UTSW 11 78,820,602 (GRCm39) missense probably benign 0.01
R4492:Nos2 UTSW 11 78,840,921 (GRCm39) missense probably benign
R4632:Nos2 UTSW 11 78,848,417 (GRCm39) missense possibly damaging 0.95
R4686:Nos2 UTSW 11 78,819,456 (GRCm39) missense possibly damaging 0.65
R5038:Nos2 UTSW 11 78,813,140 (GRCm39) missense probably benign
R5214:Nos2 UTSW 11 78,846,267 (GRCm39) missense probably damaging 1.00
R5377:Nos2 UTSW 11 78,848,317 (GRCm39) missense probably benign 0.00
R5777:Nos2 UTSW 11 78,830,978 (GRCm39) missense probably null 1.00
R5834:Nos2 UTSW 11 78,819,405 (GRCm39) missense probably benign 0.01
R5930:Nos2 UTSW 11 78,828,741 (GRCm39) missense probably damaging 1.00
R6511:Nos2 UTSW 11 78,846,290 (GRCm39) splice site probably null
R6706:Nos2 UTSW 11 78,835,549 (GRCm39) missense possibly damaging 0.60
R6747:Nos2 UTSW 11 78,843,780 (GRCm39) missense probably damaging 0.99
R6762:Nos2 UTSW 11 78,850,574 (GRCm39) missense possibly damaging 0.90
R6817:Nos2 UTSW 11 78,836,092 (GRCm39) missense possibly damaging 0.64
R6868:Nos2 UTSW 11 78,848,332 (GRCm39) missense probably benign 0.02
R6917:Nos2 UTSW 11 78,842,053 (GRCm39) missense possibly damaging 0.50
R7082:Nos2 UTSW 11 78,819,405 (GRCm39) missense probably benign 0.02
R7286:Nos2 UTSW 11 78,820,680 (GRCm39) missense probably damaging 1.00
R7367:Nos2 UTSW 11 78,840,916 (GRCm39) missense possibly damaging 0.77
R7398:Nos2 UTSW 11 78,827,297 (GRCm39) nonsense probably null
R7411:Nos2 UTSW 11 78,835,681 (GRCm39) critical splice donor site probably null
R7469:Nos2 UTSW 11 78,843,797 (GRCm39) missense possibly damaging 0.94
R8694:Nos2 UTSW 11 78,836,515 (GRCm39) missense possibly damaging 0.93
R8832:Nos2 UTSW 11 78,846,290 (GRCm39) splice site probably null
R8872:Nos2 UTSW 11 78,839,949 (GRCm39) missense probably damaging 0.99
R8952:Nos2 UTSW 11 78,836,089 (GRCm39) missense probably benign 0.00
R9433:Nos2 UTSW 11 78,850,490 (GRCm39) missense probably damaging 1.00
R9580:Nos2 UTSW 11 78,828,457 (GRCm39) missense probably benign 0.01
R9612:Nos2 UTSW 11 78,839,984 (GRCm39) missense probably damaging 1.00
R9727:Nos2 UTSW 11 78,843,825 (GRCm39) missense possibly damaging 0.51
R9747:Nos2 UTSW 11 78,822,472 (GRCm39) missense probably damaging 0.96
X0063:Nos2 UTSW 11 78,813,193 (GRCm39) missense probably benign 0.01
Z1177:Nos2 UTSW 11 78,822,498 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- CATTCTCTGGGACACTAGCG -3'
(R):5'- CTTGTCCACACCAAGGAAGG -3'

Sequencing Primer
(F):5'- CCGTGAGAAGGCTCTGTTC -3'
(R):5'- GGTGCATCAGACTCCAGTAG -3'
Posted On 2019-11-26