Mutagenetix > Incidental Mutation

Incidental Mutation 'R7749:Vmn2r12'

597086

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R7749 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109085849-109097864 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109086054 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 764 (N764S)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

probably damaging
Transcript: ENSMUST00000095922
AA Change: N764S

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: N764S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

99% (66/67)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933406M09Rik	A	T	1: 134,390,512	M341L	probably benign	Het
Actr1a	A	T	19: 46,379,747	S254T	probably benign	Het
Adam12	G	A	7: 134,224,813	A22V	unknown	Het
Adarb2	A	G	13: 8,569,739	D87G	possibly damaging	Het
Aldh6a1	A	T	12: 84,442,081	I59N	probably benign	Het
Ankrd50	C	T	3: 38,482,721	C161Y	probably damaging	Het
Ankrd7	A	G	6: 18,879,516		probably null	Het
Anks1	T	A	17: 28,038,141	I707N	probably damaging	Het
Atoh1	A	T	6: 64,729,920	M200L	possibly damaging	Het
Bmp1	C	T	14: 70,492,844	R416H	probably damaging	Het
Caprin1	A	G	2: 103,771,754	S548P	probably benign	Het
Ccdc167	T	A	17: 29,705,273	Y63F	possibly damaging	Het
Cfap99	T	A	5: 34,307,940	D174E	probably benign	Het
Chil3	T	G	3: 106,148,845	N331T	probably benign	Het
Chmp5	T	A	4: 40,949,488	I35N	probably damaging	Het
Cpt1c	A	T	7: 44,962,265	S537T	probably benign	Het
Dctn1	A	T	6: 83,186,141		probably benign	Het
Dhx57	T	A	17: 80,238,858	M1366L	probably benign	Het
Dnah9	T	A	11: 65,911,830	Y3478F	probably damaging	Het
Efna3	A	G	3: 89,316,640	Y81H	probably damaging	Het
Eif4enif1	T	C	11: 3,242,608	V812A	probably damaging	Het
Erg	A	T	16: 95,377,357	I237N	probably benign	Het
Fem1c	G	T	18: 46,524,118	N176K	probably damaging	Het
Fgd4	T	C	16: 16,475,154	Y233C	probably benign	Het
Foxd2	G	A	4: 114,907,812	A337V	unknown	Het
Gsdma2	T	C	11: 98,657,721	L433P	unknown	Het
Hmcn2	C	A	2: 31,453,033		probably null	Het
Hnrnpul2	T	C	19: 8,820,424	V48A	probably benign	Het
Hsf1	T	A	15: 76,499,187	S396T	probably benign	Het
Htr5b	G	T	1: 121,527,758	N144K	probably damaging	Het
Igkv9-120	T	C	6: 68,050,188	S29P	probably damaging	Het
Igsf10	T	A	3: 59,329,128	N1211Y	possibly damaging	Het
Kcnh1	A	C	1: 192,277,139	I334L	probably benign	Het
Laptm4b	T	C	15: 34,276,200	I158T	probably benign	Het
Muc5ac	G	C	7: 141,809,303	G2117A	unknown	Het
Nav3	T	C	10: 109,703,352	T2063A	probably damaging	Het
Nceh1	T	A	3: 27,207,382	D47E	probably benign	Het
Nckap5	A	G	1: 126,024,646	S1390P	probably damaging	Het
Npepps	T	G	11: 97,267,628	I104L	probably benign	Het
Numb	A	G	12: 83,801,277	S229P	not run	Het
Olfr1156	G	A	2: 87,949,478	H252Y	probably damaging	Het
Olfr1342	T	C	4: 118,690,228	S75G	probably damaging	Het
Olfr1443	A	T	19: 12,680,212	I35F	probably benign	Het
Opn1sw	T	A	6: 29,380,169	I83F	probably benign	Het
Pigg	T	G	5: 108,336,296	C603G	probably benign	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195		probably benign	Het
Pkhd1l1	C	A	15: 44,527,737	H1504N	probably benign	Het
Plcd4	A	G	1: 74,565,133	N757D	possibly damaging	Het
Ppia	A	G	11: 6,419,569	T152A	probably benign	Het
Psmd8	G	A	7: 29,178,921		probably null	Het
Pxn	T	A	5: 115,548,516	Y356N	probably benign	Het
Rhoa	G	C	9: 108,336,715	C159S	probably damaging	Het
Rictor	C	T	15: 6,772,154	S441L	probably benign	Het
Rita1	C	T	5: 120,611,441	C69Y	probably benign	Het
Satb1	A	G	17: 51,767,933	S512P	possibly damaging	Het
Sectm1b	A	G	11: 121,054,942	I191T	possibly damaging	Het
Slc22a6	A	G	19: 8,621,896	K297R	possibly damaging	Het
Snrnp48	T	C	13: 38,221,287	Y287H	probably benign	Het
Sntg2	A	T	12: 30,226,911	C381S	probably benign	Het
Syngap1	T	A	17: 26,959,964	M649K	probably damaging	Het
Taf4	G	A	2: 179,932,029	T682M	probably damaging	Het
Thap2	T	C	10: 115,376,384	I79V	probably damaging	Het
Thap7	T	C	16: 17,528,603	N172S	probably benign	Het
Ticrr	A	G	7: 79,679,096	Y661C	possibly damaging	Het
Ttn	T	C	2: 76,776,315	N18084D	probably damaging	Het
Usp48	A	T	4: 137,650,417	K1020M	probably damaging	Het
Vmn2r91	A	G	17: 18,136,278	I736V	possibly damaging	Het
Wfdc6b	T	A	2: 164,617,419	C134S	probably damaging	Het
Zcchc2	A	T	1: 106,018,273	D481V	probably damaging	Het
Zfp949	G	A	9: 88,569,870	G498R	probably damaging	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109097675	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109086259	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109091850	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109093027	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109086564	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109092159	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109086477	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109085992	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109090485	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109092070	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109092899	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109091889	nonsense	probably null
R0529:Vmn2r12	UTSW	5	109092848	missense	probably benign
R0715:Vmn2r12	UTSW	5	109090507	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109086415	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109087850	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109092854	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109092854	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109091897	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109086586	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109092974	missense	possibly damaging	0.56
R1549:Vmn2r12	UTSW	5	109092830	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109092044	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109091728	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109092076	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109091474	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109086532	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109091531	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109091531	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109090504	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109092192	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109092192	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109091546	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109091964	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109086006	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109086435	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109086513	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109092986	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109091678	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109091506	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109090395	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109091818	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109086617	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109092800	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109091804	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109085870	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109092897	missense	probably benign
R6162:Vmn2r12	UTSW	5	109086564	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109086000	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109092905	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109097789	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109086247	missense	possibly damaging	0.74
R7341:Vmn2r12	UTSW	5	109091945	missense	possibly damaging	0.95
R7383:Vmn2r12	UTSW	5	109092818	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109091749	missense	probably damaging	1.00
R7861:Vmn2r12	UTSW	5	109087963	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109086441	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109091881	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109090483	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109086208	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109092086	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109086656	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109086167	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109086019	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109093044	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109086337	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109086586	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109086120	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109091957	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109091456	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109092780	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109091437	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTGGGAGAAAGATGCACCC -3'
(R):5'- GGCACCTAACTTGGTCATTCCC -3'

Sequencing Primer
(F):5'- TGCACCCTAGCAAGCCTG -3'
(R):5'- AATCCAACTTGTCCTCTGTGG -3'

Posted On

2019-11-26