Mutagenetix > Incidental Mutation

Incidental Mutation 'R7755:Neto2'

597500

Institutional Source

Beutler Lab

Gene Symbol

Neto2

Ensembl Gene

ENSMUSG00000036902

Gene Name

neuropilin (NRP) and tolloid (TLL)-like 2

Synonyms

5530601C23Rik

MMRRC Submission

045811-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.157) question?

Stock #

R7755 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

86363217-86427553 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 86396285 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 155 (R155H)

Ref Sequence

ENSEMBL: ENSMUSP00000105308 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109686] [ENSMUST00000209479] [ENSMUST00000216286]

AlphaFold

Q8BNJ6

Predicted Effect

probably damaging
Transcript: ENSMUST00000109686
AA Change: R155H

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000105308
Gene: ENSMUSG00000036902
AA Change: R155H

Domain	Start	End	E-Value	Type
transmembrane domain	38	60	N/A	INTRINSIC
CUB	80	194	2.56e-40	SMART
CUB	205	320	9.11e-5	SMART
LDLa	324	361	5.73e-5	SMART
transmembrane domain	374	396	N/A	INTRINSIC
coiled coil region	432	460	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000209479
AA Change: R120H

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216286
AA Change: R120H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.5514

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (52/53)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in rats encodes a protein that modulates glutamate signaling in the brain by regulating kainate receptor function. Expression of this gene may be a biomarker for proliferating infantile hemangiomas. A pseudogene of this gene is located on the long arm of chromosome 8. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
PHENOTYPE: Mice homozygous for a null mutation show normal brain morphology and kainate receptor mediated excitatory postsynaptic currents. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	A	12: 71,236,187 (GRCm39)	M1179I	probably benign	Het
4930402F06Rik	T	C	2: 35,266,349 (GRCm39)	Y107C	probably damaging	Het
Aff4	T	C	11: 53,289,206 (GRCm39)	S452P	probably damaging	Het
Astn2	T	C	4: 65,712,795 (GRCm39)	D615G	probably damaging	Het
Begain	A	G	12: 109,018,802 (GRCm39)	L215P	probably benign	Het
Carf	T	A	1: 60,187,214 (GRCm39)	S606T	probably benign	Het
Cngb3	A	T	4: 19,461,684 (GRCm39)	T522S	probably benign	Het
Ctsll3	A	T	13: 60,948,219 (GRCm39)	F153I	probably damaging	Het
Cubn	G	A	2: 13,284,889 (GRCm39)	T3509I	probably benign	Het
Dmrta1	T	C	4: 89,580,170 (GRCm39)	S377P	probably benign	Het
Dnmbp	G	A	19: 43,838,525 (GRCm39)	A659V	probably benign	Het
Dspp	TGACAGCAGTGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAGCGACAGCAGCAACAGCAGTGACAGCAG	TGACAGCAGTGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAGCGACAGCAGCAACAGCAGTGACAGCAG	5: 104,326,227 (GRCm39)		probably benign	Het
Dync2h1	T	C	9: 7,015,490 (GRCm39)	D3598G	probably benign	Het
Elp1	T	C	4: 56,774,552 (GRCm39)	N779S	possibly damaging	Het
Flot2	T	C	11: 77,940,339 (GRCm39)	F29L	probably benign	Het
Hectd1	A	T	12: 51,849,003 (GRCm39)	I367K	possibly damaging	Het
Ivl	T	A	3: 92,479,317 (GRCm39)	K249N	probably damaging	Het
Khnyn	A	G	14: 56,125,425 (GRCm39)	T503A	probably damaging	Het
Mbd4	T	A	6: 115,821,546 (GRCm39)	I490F	probably damaging	Het
Mlip	T	C	9: 77,136,838 (GRCm39)	T690A	probably benign	Het
Mmp1a	T	A	9: 7,467,005 (GRCm39)	D227E	possibly damaging	Het
Mrgprf	T	C	7: 144,862,380 (GRCm39)	L314P	probably damaging	Het
Ndufb9	T	G	15: 58,808,255 (GRCm39)	Y80*	probably null	Het
Npat	T	A	9: 53,470,470 (GRCm39)	N365K	possibly damaging	Het
Nsl1	G	A	1: 190,795,380 (GRCm39)	V49M	probably benign	Het
Nudt21	T	A	8: 94,749,493 (GRCm39)	Y191F	probably benign	Het
Or2w25	A	G	11: 59,504,467 (GRCm39)	R226G	probably damaging	Het
Or8b54	T	G	9: 38,687,073 (GRCm39)	I174S	possibly damaging	Het
Pcdh18	T	C	3: 49,709,278 (GRCm39)	Y679C	possibly damaging	Het
Pkd1l3	A	G	8: 110,356,798 (GRCm39)	D741G	possibly damaging	Het
Pkhd1	T	C	1: 20,617,717 (GRCm39)	D956G	probably damaging	Het
Poglut2	T	C	1: 44,157,733 (GRCm39)		probably benign	Het
Pparg	C	T	6: 115,440,067 (GRCm39)	P214S	probably damaging	Het
Ppargc1a	T	A	5: 51,630,883 (GRCm39)	Y582F	unknown	Het
Prdm9	C	T	17: 15,765,226 (GRCm39)	C518Y	probably damaging	Het
Rangrf	T	A	11: 68,864,540 (GRCm39)	E2V	probably damaging	Het
Rpl19	T	C	11: 97,919,193 (GRCm39)	I45T	probably benign	Het
Rtn4rl2	T	C	2: 84,702,807 (GRCm39)	D255G	possibly damaging	Het
Shld2	T	C	14: 33,970,847 (GRCm39)	K627E	probably damaging	Het
Sirt3	T	C	7: 140,457,963 (GRCm39)	D62G		Het
Slc22a30	T	C	19: 8,314,133 (GRCm39)	T518A	probably damaging	Het
Slc40a1	T	C	1: 45,950,466 (GRCm39)	T329A	probably damaging	Het
Slc6a17	C	T	3: 107,381,671 (GRCm39)	G470D	probably damaging	Het
Syne2	A	T	12: 76,044,181 (GRCm39)	I3923L	probably benign	Het
Tiam2	C	T	17: 3,471,591 (GRCm39)	S411L	probably benign	Het
Ticam1	A	G	17: 56,577,182 (GRCm39)	C638R	unknown	Het
Tnrc6c	A	G	11: 117,648,912 (GRCm39)	T1528A	probably benign	Het
Ufl1	A	G	4: 25,262,274 (GRCm39)	I404T	probably benign	Het
Usp43	A	T	11: 67,782,294 (GRCm39)	S375T	possibly damaging	Het
Vmn2r88	C	G	14: 51,650,503 (GRCm39)	A72G	probably benign	Het
Vmn2r91	A	T	17: 18,330,311 (GRCm39)	I532F	possibly damaging	Het
Vmn2r95	T	C	17: 18,644,367 (GRCm39)	M1T	probably null	Het
Xirp2	T	C	2: 67,345,526 (GRCm39)	V2589A	probably benign	Het
Zfp128	C	T	7: 12,624,240 (GRCm39)	Q203*	probably null	Het

Other mutations in Neto2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01705:Neto2	APN	8	86,367,632 (GRCm39)	missense	probably damaging	1.00
IGL01938:Neto2	APN	8	86,417,484 (GRCm39)	missense	probably benign	0.00
IGL02238:Neto2	APN	8	86,396,292 (GRCm39)	missense	probably damaging	0.99
IGL02605:Neto2	APN	8	86,390,064 (GRCm39)	splice site	probably benign
IGL02813:Neto2	APN	8	86,417,515 (GRCm39)	missense	probably benign
R0138:Neto2	UTSW	8	86,367,673 (GRCm39)	missense	possibly damaging	0.72
R1934:Neto2	UTSW	8	86,397,033 (GRCm39)	missense	possibly damaging	0.96
R2402:Neto2	UTSW	8	86,417,541 (GRCm39)	missense	probably benign	0.00
R2423:Neto2	UTSW	8	86,396,396 (GRCm39)	missense	probably damaging	1.00
R3821:Neto2	UTSW	8	86,389,924 (GRCm39)	nonsense	probably null
R3822:Neto2	UTSW	8	86,389,924 (GRCm39)	nonsense	probably null
R3883:Neto2	UTSW	8	86,389,894 (GRCm39)	missense	probably damaging	1.00
R3939:Neto2	UTSW	8	86,400,747 (GRCm39)	missense	probably damaging	0.99
R3940:Neto2	UTSW	8	86,400,747 (GRCm39)	missense	probably damaging	0.99
R3941:Neto2	UTSW	8	86,400,747 (GRCm39)	missense	probably damaging	0.99
R4433:Neto2	UTSW	8	86,367,712 (GRCm39)	missense	probably damaging	1.00
R4668:Neto2	UTSW	8	86,367,691 (GRCm39)	missense	probably damaging	1.00
R4675:Neto2	UTSW	8	86,396,333 (GRCm39)	missense	probably damaging	1.00
R4908:Neto2	UTSW	8	86,396,393 (GRCm39)	missense	probably damaging	0.99
R5459:Neto2	UTSW	8	86,397,112 (GRCm39)	missense	probably benign	0.35
R5471:Neto2	UTSW	8	86,367,389 (GRCm39)	missense	probably benign	0.41
R5544:Neto2	UTSW	8	86,374,506 (GRCm39)	missense	possibly damaging	0.94
R5571:Neto2	UTSW	8	86,367,173 (GRCm39)	missense	probably damaging	1.00
R6083:Neto2	UTSW	8	86,367,214 (GRCm39)	missense	probably benign	0.00
R6339:Neto2	UTSW	8	86,367,187 (GRCm39)	missense	probably benign	0.33
R6381:Neto2	UTSW	8	86,369,138 (GRCm39)	missense	probably damaging	0.99
R6572:Neto2	UTSW	8	86,397,033 (GRCm39)	missense	possibly damaging	0.96
R6593:Neto2	UTSW	8	86,396,175 (GRCm39)	missense	probably damaging	1.00
R6662:Neto2	UTSW	8	86,389,844 (GRCm39)	missense	probably damaging	1.00
R6881:Neto2	UTSW	8	86,367,185 (GRCm39)	missense	probably damaging	1.00
R6950:Neto2	UTSW	8	86,397,072 (GRCm39)	missense	probably damaging	1.00
R7121:Neto2	UTSW	8	86,397,020 (GRCm39)	splice site	probably null
R7754:Neto2	UTSW	8	86,396,329 (GRCm39)	missense	probably damaging	0.98
R8682:Neto2	UTSW	8	86,367,295 (GRCm39)	missense	probably benign	0.01
R9326:Neto2	UTSW	8	86,369,063 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAATGGTATATGGCCTATTGTTCC -3'
(R):5'- GTTCCCAGTGTAATTCTCTGATATG -3'

Sequencing Primer
(F):5'- TACTGGGAATTGAACTCAGGACCTC -3'
(R):5'- GCACTAAAGAGGTGGATTTGTTTATC -3'

Posted On

2019-11-26