Mutagenetix > Incidental Mutation

Incidental Mutation 'R7757:Mycbp2'

597640

Institutional Source

Beutler Lab

Gene Symbol

Mycbp2

Ensembl Gene

ENSMUSG00000033004

Gene Name

MYC binding protein 2

Synonyms

C130061D10Rik, Phr1, Pam

MMRRC Submission

045813-MU

Accession Numbers

Genbank: NM_207215; MGI: 2179432

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7757 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

103113411-103346814 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 103191619 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 2374 (Y2374N)

Ref Sequence

ENSEMBL: ENSMUSP00000124710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159855] [ENSMUST00000160758]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000159855
AA Change: Y2374N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124710
Gene: ENSMUSG00000033004
AA Change: Y2374N

Domain	Start	End	E-Value	Type
low complexity region	5	27	N/A	INTRINSIC
low complexity region	47	55	N/A	INTRINSIC
low complexity region	100	127	N/A	INTRINSIC
low complexity region	178	191	N/A	INTRINSIC
Pfam:RCC1_2	683	712	1.4e-10	PFAM
low complexity region	737	750	N/A	INTRINSIC
low complexity region	793	815	N/A	INTRINSIC
Pfam:RCC1_2	942	971	5.5e-10	PFAM
Pfam:RCC1	958	1006	4.8e-15	PFAM
Pfam:PHR	1235	1385	8.2e-44	PFAM
Pfam:PHR	1723	1880	1.4e-43	PFAM
low complexity region	1935	1948	N/A	INTRINSIC
low complexity region	2182	2195	N/A	INTRINSIC
Pfam:Filamin	2261	2431	7.5e-9	PFAM
Pfam:SH3_3	2472	2539	4.1e-9	PFAM
internal_repeat_3	2612	2679	1.69e-7	PROSPERO
low complexity region	2701	2710	N/A	INTRINSIC
low complexity region	2884	2917	N/A	INTRINSIC
low complexity region	2970	2984	N/A	INTRINSIC
coiled coil region	3263	3290	N/A	INTRINSIC
low complexity region	3352	3365	N/A	INTRINSIC
low complexity region	3418	3433	N/A	INTRINSIC
low complexity region	3678	3695	N/A	INTRINSIC
APC10	3810	3968	1.11e-18	SMART
low complexity region	4103	4115	N/A	INTRINSIC
low complexity region	4190	4212	N/A	INTRINSIC
Blast:BBOX	4327	4370	7e-7	BLAST
RING	4496	4546	5.35e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000160758
AA Change: Y2341N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124601
Gene: ENSMUSG00000033004
AA Change: Y2341N

Domain	Start	End	E-Value	Type
low complexity region	14	22	N/A	INTRINSIC
low complexity region	67	94	N/A	INTRINSIC
low complexity region	145	158	N/A	INTRINSIC
Pfam:RCC1_2	650	679	1e-10	PFAM
low complexity region	704	717	N/A	INTRINSIC
low complexity region	760	782	N/A	INTRINSIC
Pfam:RCC1_2	909	938	1.5e-9	PFAM
Pfam:RCC1	925	973	1.3e-15	PFAM
Pfam:PHR	1202	1353	1.6e-50	PFAM
Pfam:PHR	1690	1848	3.1e-58	PFAM
low complexity region	1902	1915	N/A	INTRINSIC
low complexity region	2149	2162	N/A	INTRINSIC
Pfam:Filamin	2228	2398	7.6e-9	PFAM
Pfam:SH3_3	2439	2507	2.3e-10	PFAM
internal_repeat_3	2554	2621	2e-7	PROSPERO
low complexity region	2643	2652	N/A	INTRINSIC
low complexity region	2774	2807	N/A	INTRINSIC
low complexity region	2860	2874	N/A	INTRINSIC
coiled coil region	3153	3180	N/A	INTRINSIC
low complexity region	3242	3255	N/A	INTRINSIC
low complexity region	3308	3323	N/A	INTRINSIC
low complexity region	3568	3585	N/A	INTRINSIC
APC10	3700	3858	1.11e-18	SMART
low complexity region	3993	4005	N/A	INTRINSIC
low complexity region	4080	4102	N/A	INTRINSIC
Blast:BBOX	4217	4260	7e-7	BLAST
RING	4386	4436	5.35e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000161278

SMART Domains

Protein: ENSMUSP00000125189
Gene: ENSMUSG00000033004

Domain	Start	End	E-Value	Type
Blast:IG_FLMN	2	66	1e-37	BLAST
SCOP:d1qfha1	14	64	2e-7	SMART
Pfam:SH3_3	98	165	5.8e-10	PFAM
internal_repeat_1	213	280	8.77e-8	PROSPERO
low complexity region	302	311	N/A	INTRINSIC
low complexity region	485	518	N/A	INTRINSIC
low complexity region	571	585	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

96% (70/73)

MGI Phenotype

PHENOTYPE: Mice homozygous for a targeted allele exhibit neonatal lethality, defective diaphragm innervation, abnormal brain morphology and defective axonal guidance. [provided by MGI curators]

Allele List at MGI

All alleles(10) : Targeted, knock-out(1) Targeted, other(1) Gene trapped(5) Chemically induced(3)

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	A	T	15: 8,252,227	E2850V	unknown	Het
2700049A03Rik	G	A	12: 71,189,413	M1179I	probably benign	Het
6430550D23Rik	T	C	2: 156,003,431	T18A	possibly damaging	Het
Acox2	T	C	14: 8,230,166	N659S	probably damaging	Het
Agk	T	C	6: 40,376,278	V192A	possibly damaging	Het
Ap3b1	T	A	13: 94,528,158		probably null	Het
Bche	T	A	3: 73,701,121	D324V	probably damaging	Het
Bsn	A	G	9: 108,114,740	I1271T	possibly damaging	Het
Capn12	T	C	7: 28,882,821	L120P	probably damaging	Het
Cep290	T	A	10: 100,563,434	S2273T	probably benign	Het
Ckap4	T	C	10: 84,528,467	E244G	probably damaging	Het
Clcn7	T	C	17: 25,156,822	Y545H	probably damaging	Het
Cmya5	T	A	13: 93,098,272	T103S	possibly damaging	Het
Cpne9	G	A	6: 113,284,445	V121M	possibly damaging	Het
Csmd2	T	A	4: 128,483,456	I2043N		Het
Disc1	A	G	8: 125,087,504	T36A	probably benign	Het
Disp2	T	C	2: 118,790,910	Y708H	probably damaging	Het
Dnah3	A	G	7: 120,071,570	V635A	probably benign	Het
Dnah3	A	T	7: 119,971,215		probably null	Het
Dync1li1	A	G	9: 114,709,277	H234R	possibly damaging	Het
Egfr	T	C	11: 16,889,966	V660A	possibly damaging	Het
Epb42	T	C	2: 121,027,719	R253G	possibly damaging	Het
Fat2	T	A	11: 55,311,421	T276S	probably benign	Het
Fcho2	A	G	13: 98,764,503		probably null	Het
Ginm1	A	T	10: 7,779,355	I41N	probably damaging	Het
Gm4744	A	G	6: 40,950,433		probably benign	Het
Gm49368	C	T	7: 128,112,226	R701C	probably damaging	Het
Gpr37	T	A	6: 25,688,208	I297F	probably benign	Het
Gprc5a	T	A	6: 135,079,344	I263N	possibly damaging	Het
Gys2	A	T	6: 142,454,451	S345T	probably benign	Het
Hk2	T	C	6: 82,742,915	M255V	possibly damaging	Het
Ibtk	A	C	9: 85,697,237	S1202A	possibly damaging	Het
Il23r	T	G	6: 67,423,981	D455A	probably benign	Het
Irs2	T	A	8: 11,006,522	K637*	probably null	Het
Jak2	T	C	19: 29,283,546	V314A	probably benign	Het
Mei1	C	T	15: 82,082,623		probably benign	Het
Mill1	T	C	7: 18,262,466	M69T	probably benign	Het
Mms22l	T	C	4: 24,598,884		probably null	Het
Mup8	C	A	4: 60,220,332	Q133H	probably benign	Het
Mup8	T	A	4: 60,220,333	Q133L	probably benign	Het
Nbeal1	A	T	1: 60,257,450	K1166N	probably damaging	Het
Nlrc4	T	A	17: 74,448,196	R8S	probably benign	Het
Nrcam	C	T	12: 44,549,898	Q25*	probably null	Het
Nudt16	A	C	9: 105,131,561	M47R	probably damaging	Het
Nup62	T	C	7: 44,828,995	S145P	probably benign	Het
Olfr1222	T	C	2: 89,124,989	I247M	possibly damaging	Het
Olfr959	A	G	9: 39,572,465	W265R	probably benign	Het
Osbpl1a	A	T	18: 12,933,600	V34D	probably benign	Het
Otogl	T	A	10: 107,876,921	N521Y	probably damaging	Het
Pcdhb14	T	A	18: 37,449,834	D664E	possibly damaging	Het
Pex5l	T	C	3: 33,082,151		probably benign	Het
Pkhd1	A	G	1: 20,562,415	L592P	probably damaging	Het
Plxdc2	A	G	2: 16,729,376	H480R	probably benign	Het
Rnf216	T	A	5: 143,080,236	K532N	probably damaging	Het
Schip1	C	A	3: 68,617,695	Q358K	probably damaging	Het
Sdc2	A	T	15: 33,028,087	E117V	possibly damaging	Het
Sept11	A	T	5: 93,171,464		probably null	Het
Shprh	A	T	10: 11,162,180	E420V	probably benign	Het
Ska1	T	A	18: 74,196,973	H232L	probably benign	Het
Slc36a4	A	G	9: 15,719,660	N25S	possibly damaging	Het
Slitrk3	G	A	3: 73,050,839	T200M	probably damaging	Het
Smad2	C	T	18: 76,288,013	H138Y	probably benign	Het
Snx21	T	C	2: 164,786,165	S34P	probably damaging	Het
Sos2	A	G	12: 69,648,585	V126A	probably damaging	Het
Srcap	C	T	7: 127,530,794	T596I	probably damaging	Het
Stk31	T	G	6: 49,406,943		probably null	Het
Stox1	T	C	10: 62,663,964	D939G	probably damaging	Het
Syne2	G	T	12: 76,061,779	C979F	possibly damaging	Het
Tanc2	C	A	11: 105,776,858	N88K	possibly damaging	Het
Tll2	A	T	19: 41,096,008	V677E	probably damaging	Het
Ttn	T	C	2: 76,918,490	T4072A	probably benign	Het
Unc13b	CAGAGC	CAGAGCGAGAGC	4: 43,177,341		probably benign	Het
Unc13b	AGAGCC	AGAGCCCGAGCC	4: 43,177,330		probably benign	Het
Unc13b	CGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGC	CGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGC	4: 43,177,312		probably benign	Het
Vmn2r43	A	T	7: 8,255,254	F320Y	possibly damaging	Het
Zscan4f	G	T	7: 11,401,278	G204*	probably null	Het

Other mutations in Mycbp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Mycbp2	APN	14	103,223,050 (GRCm38)	missense	probably damaging	1.00
IGL00518:Mycbp2	APN	14	103,155,808 (GRCm38)	missense	probably damaging	1.00
IGL00650:Mycbp2	APN	14	103,143,228 (GRCm38)	missense	probably damaging	0.97
IGL00653:Mycbp2	APN	14	103,143,228 (GRCm38)	missense	probably damaging	0.97
IGL00742:Mycbp2	APN	14	103,201,352 (GRCm38)	missense	probably damaging	1.00
IGL00755:Mycbp2	APN	14	103,194,621 (GRCm38)	missense	possibly damaging	0.72
IGL00793:Mycbp2	APN	14	103,126,753 (GRCm38)	missense	possibly damaging	0.77
IGL00916:Mycbp2	APN	14	103,291,283 (GRCm38)	splice site	probably benign
IGL00960:Mycbp2	APN	14	103,229,384 (GRCm38)	missense	possibly damaging	0.95
IGL00977:Mycbp2	APN	14	103,172,642 (GRCm38)	missense	probably damaging	0.98
IGL01349:Mycbp2	APN	14	103,122,547 (GRCm38)	missense	probably damaging	0.98
IGL01369:Mycbp2	APN	14	103,155,510 (GRCm38)	missense	possibly damaging	0.61
IGL01410:Mycbp2	APN	14	103,229,492 (GRCm38)	splice site	probably null
IGL01586:Mycbp2	APN	14	103,140,869 (GRCm38)	critical splice donor site	probably null
IGL01593:Mycbp2	APN	14	103,291,287 (GRCm38)	critical splice donor site	probably null
IGL01693:Mycbp2	APN	14	103,127,979 (GRCm38)	missense	probably damaging	0.99
IGL01730:Mycbp2	APN	14	103,135,204 (GRCm38)	nonsense	probably null
IGL01820:Mycbp2	APN	14	103,188,501 (GRCm38)	missense	probably damaging	1.00
IGL01974:Mycbp2	APN	14	103,143,211 (GRCm38)	missense	possibly damaging	0.88
IGL02071:Mycbp2	APN	14	103,154,907 (GRCm38)	nonsense	probably null
IGL02178:Mycbp2	APN	14	103,224,366 (GRCm38)	missense	probably benign	0.01
IGL02324:Mycbp2	APN	14	103,242,207 (GRCm38)	missense	probably damaging	1.00
IGL02442:Mycbp2	APN	14	103,314,375 (GRCm38)	missense	probably benign
IGL02607:Mycbp2	APN	14	103,285,273 (GRCm38)	missense	probably damaging	1.00
IGL02679:Mycbp2	APN	14	103,205,185 (GRCm38)	missense	probably benign
IGL02702:Mycbp2	APN	14	103,220,124 (GRCm38)	missense	probably benign	0.01
IGL02709:Mycbp2	APN	14	103,155,261 (GRCm38)	missense	probably damaging	0.97
IGL02736:Mycbp2	APN	14	103,114,242 (GRCm38)	splice site	probably benign
IGL02866:Mycbp2	APN	14	103,129,992 (GRCm38)	missense	probably damaging	0.98
IGL02939:Mycbp2	APN	14	103,177,279 (GRCm38)	missense	probably benign
IGL03082:Mycbp2	APN	14	103,204,369 (GRCm38)	missense	probably benign	0.23
IGL03142:Mycbp2	APN	14	103,298,776 (GRCm38)	missense	probably damaging	0.99
IGL03155:Mycbp2	APN	14	103,155,453 (GRCm38)	missense	probably benign	0.06
IGL03236:Mycbp2	APN	14	103,298,698 (GRCm38)	missense	probably damaging	0.99
IGL03256:Mycbp2	APN	14	103,188,589 (GRCm38)	missense	possibly damaging	0.92
IGL03303:Mycbp2	APN	14	103,247,758 (GRCm38)	missense	probably damaging	1.00
compost	UTSW	14	103,156,453 (GRCm38)	missense	probably damaging	1.00
decompose	UTSW	14	103,219,979 (GRCm38)	missense	probably benign	0.12
moulder	UTSW	14	103,188,592 (GRCm38)	missense	probably damaging	1.00
N/A - 293:Mycbp2	UTSW	14	103,224,462 (GRCm38)	splice site	probably benign
R0040:Mycbp2	UTSW	14	103,224,272 (GRCm38)	missense	probably benign	0.11
R0040:Mycbp2	UTSW	14	103,224,272 (GRCm38)	missense	probably benign	0.11
R0057:Mycbp2	UTSW	14	103,152,142 (GRCm38)	missense	probably damaging	0.97
R0063:Mycbp2	UTSW	14	103,156,634 (GRCm38)	unclassified	probably benign
R0097:Mycbp2	UTSW	14	103,155,762 (GRCm38)	missense	probably damaging	1.00
R0097:Mycbp2	UTSW	14	103,155,762 (GRCm38)	missense	probably damaging	1.00
R0268:Mycbp2	UTSW	14	103,314,325 (GRCm38)	nonsense	probably null
R0388:Mycbp2	UTSW	14	103,156,667 (GRCm38)	missense	probably benign	0.01
R0410:Mycbp2	UTSW	14	103,135,133 (GRCm38)	missense	probably damaging	1.00
R0530:Mycbp2	UTSW	14	103,182,459 (GRCm38)	missense	probably damaging	1.00
R0591:Mycbp2	UTSW	14	103,196,391 (GRCm38)	unclassified	probably benign
R0671:Mycbp2	UTSW	14	103,194,588 (GRCm38)	missense	possibly damaging	0.95
R0755:Mycbp2	UTSW	14	103,174,794 (GRCm38)	missense	probably damaging	1.00
R0817:Mycbp2	UTSW	14	103,229,418 (GRCm38)	missense	probably damaging	0.99
R0818:Mycbp2	UTSW	14	103,229,418 (GRCm38)	missense	probably damaging	0.99
R0819:Mycbp2	UTSW	14	103,229,418 (GRCm38)	missense	probably damaging	0.99
R0881:Mycbp2	UTSW	14	103,220,013 (GRCm38)	missense	probably benign
R0903:Mycbp2	UTSW	14	103,275,857 (GRCm38)	missense	probably damaging	0.99
R0940:Mycbp2	UTSW	14	103,262,693 (GRCm38)	unclassified	probably benign
R0961:Mycbp2	UTSW	14	103,184,835 (GRCm38)	missense	probably damaging	1.00
R1004:Mycbp2	UTSW	14	103,140,917 (GRCm38)	missense	probably benign	0.00
R1138:Mycbp2	UTSW	14	103,174,826 (GRCm38)	missense	possibly damaging	0.84
R1170:Mycbp2	UTSW	14	103,200,152 (GRCm38)	nonsense	probably null
R1211:Mycbp2	UTSW	14	103,120,563 (GRCm38)	missense	probably benign	0.31
R1268:Mycbp2	UTSW	14	103,208,782 (GRCm38)	missense	probably damaging	1.00
R1298:Mycbp2	UTSW	14	103,155,898 (GRCm38)	missense	probably damaging	1.00
R1341:Mycbp2	UTSW	14	103,298,867 (GRCm38)	splice site	probably benign
R1469:Mycbp2	UTSW	14	103,188,520 (GRCm38)	missense	probably damaging	0.99
R1469:Mycbp2	UTSW	14	103,188,520 (GRCm38)	missense	probably damaging	0.99
R1513:Mycbp2	UTSW	14	103,204,389 (GRCm38)	missense	probably damaging	1.00
R1528:Mycbp2	UTSW	14	103,232,597 (GRCm38)	missense	possibly damaging	0.91
R1564:Mycbp2	UTSW	14	103,169,851 (GRCm38)	splice site	probably null
R1565:Mycbp2	UTSW	14	103,252,509 (GRCm38)	missense	possibly damaging	0.82
R1656:Mycbp2	UTSW	14	103,247,758 (GRCm38)	missense	probably damaging	1.00
R1694:Mycbp2	UTSW	14	103,227,511 (GRCm38)	missense	probably damaging	1.00
R1709:Mycbp2	UTSW	14	103,224,416 (GRCm38)	missense	probably damaging	1.00
R1728:Mycbp2	UTSW	14	103,155,178 (GRCm38)	missense	probably damaging	0.98
R1751:Mycbp2	UTSW	14	103,248,405 (GRCm38)	missense	probably damaging	0.98
R1767:Mycbp2	UTSW	14	103,248,405 (GRCm38)	missense	probably damaging	0.98
R1772:Mycbp2	UTSW	14	103,182,419 (GRCm38)	missense	probably damaging	1.00
R1784:Mycbp2	UTSW	14	103,155,178 (GRCm38)	missense	probably damaging	0.98
R1823:Mycbp2	UTSW	14	103,252,509 (GRCm38)	missense	possibly damaging	0.82
R1824:Mycbp2	UTSW	14	103,252,509 (GRCm38)	missense	possibly damaging	0.82
R1844:Mycbp2	UTSW	14	103,155,714 (GRCm38)	missense	possibly damaging	0.94
R1916:Mycbp2	UTSW	14	103,184,883 (GRCm38)	missense	probably damaging	1.00
R1944:Mycbp2	UTSW	14	103,229,404 (GRCm38)	missense	probably damaging	1.00
R1983:Mycbp2	UTSW	14	103,145,971 (GRCm38)	missense	probably damaging	0.97
R2002:Mycbp2	UTSW	14	103,248,403 (GRCm38)	missense	probably damaging	0.98
R2031:Mycbp2	UTSW	14	103,188,592 (GRCm38)	missense	probably damaging	1.00
R2035:Mycbp2	UTSW	14	103,260,239 (GRCm38)	missense	probably damaging	1.00
R2048:Mycbp2	UTSW	14	103,232,524 (GRCm38)	critical splice donor site	probably null
R2061:Mycbp2	UTSW	14	103,287,260 (GRCm38)	missense	probably damaging	0.99
R2113:Mycbp2	UTSW	14	103,220,076 (GRCm38)	missense	probably damaging	0.99
R2128:Mycbp2	UTSW	14	103,201,230 (GRCm38)	missense	probably benign	0.01
R2134:Mycbp2	UTSW	14	103,208,893 (GRCm38)	missense	probably damaging	1.00
R2135:Mycbp2	UTSW	14	103,145,942 (GRCm38)	missense	probably benign
R2135:Mycbp2	UTSW	14	103,208,893 (GRCm38)	missense	probably damaging	1.00
R2146:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2147:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2148:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2150:Mycbp2	UTSW	14	103,155,922 (GRCm38)	missense	probably damaging	0.97
R2163:Mycbp2	UTSW	14	103,169,855 (GRCm38)	critical splice donor site	probably null
R2248:Mycbp2	UTSW	14	103,169,859 (GRCm38)	missense	possibly damaging	0.50
R2265:Mycbp2	UTSW	14	103,262,749 (GRCm38)	missense	probably benign	0.39
R2272:Mycbp2	UTSW	14	103,144,338 (GRCm38)	missense	probably null	0.66
R2379:Mycbp2	UTSW	14	103,174,950 (GRCm38)	missense	probably benign
R2495:Mycbp2	UTSW	14	103,200,118 (GRCm38)	missense	probably damaging	0.99
R2508:Mycbp2	UTSW	14	103,131,245 (GRCm38)	missense	probably damaging	0.99
R2510:Mycbp2	UTSW	14	103,155,255 (GRCm38)	missense	probably damaging	0.96
R2851:Mycbp2	UTSW	14	103,144,333 (GRCm38)	missense	probably damaging	0.99
R2852:Mycbp2	UTSW	14	103,144,333 (GRCm38)	missense	probably damaging	0.99
R2965:Mycbp2	UTSW	14	103,297,358 (GRCm38)	missense	probably benign	0.00
R3156:Mycbp2	UTSW	14	103,208,743 (GRCm38)	splice site	probably benign
R3404:Mycbp2	UTSW	14	103,200,114 (GRCm38)	missense	probably damaging	0.99
R3410:Mycbp2	UTSW	14	103,135,117 (GRCm38)	missense	probably damaging	1.00
R3429:Mycbp2	UTSW	14	103,229,430 (GRCm38)	missense	probably damaging	1.00
R3706:Mycbp2	UTSW	14	103,156,414 (GRCm38)	missense	probably benign	0.31
R3772:Mycbp2	UTSW	14	103,133,788 (GRCm38)	missense	possibly damaging	0.82
R3778:Mycbp2	UTSW	14	103,197,285 (GRCm38)	missense	probably damaging	0.99
R3883:Mycbp2	UTSW	14	103,295,250 (GRCm38)	missense	probably damaging	0.97
R3884:Mycbp2	UTSW	14	103,295,250 (GRCm38)	missense	probably damaging	0.97
R3887:Mycbp2	UTSW	14	103,174,797 (GRCm38)	missense	probably damaging	0.98
R3923:Mycbp2	UTSW	14	103,126,713 (GRCm38)	missense	probably damaging	1.00
R3926:Mycbp2	UTSW	14	103,204,500 (GRCm38)	missense	probably damaging	1.00
R3959:Mycbp2	UTSW	14	103,295,252 (GRCm38)	missense	probably benign	0.00
R3966:Mycbp2	UTSW	14	103,138,725 (GRCm38)	splice site	probably benign
R4021:Mycbp2	UTSW	14	103,152,157 (GRCm38)	missense	probably damaging	0.97
R4363:Mycbp2	UTSW	14	103,248,457 (GRCm38)	missense	probably damaging	1.00
R4405:Mycbp2	UTSW	14	103,123,445 (GRCm38)	missense	probably damaging	1.00
R4407:Mycbp2	UTSW	14	103,287,228 (GRCm38)	missense	probably damaging	1.00
R4410:Mycbp2	UTSW	14	103,135,266 (GRCm38)	missense	probably damaging	1.00
R4434:Mycbp2	UTSW	14	103,133,789 (GRCm38)	missense	probably damaging	0.99
R4448:Mycbp2	UTSW	14	103,188,502 (GRCm38)	missense	possibly damaging	0.89
R4452:Mycbp2	UTSW	14	103,155,658 (GRCm38)	missense	probably damaging	0.99
R4573:Mycbp2	UTSW	14	103,346,297 (GRCm38)	missense	probably benign	0.05
R4589:Mycbp2	UTSW	14	103,177,313 (GRCm38)	missense	probably benign	0.04
R4621:Mycbp2	UTSW	14	103,219,979 (GRCm38)	missense	probably benign	0.12
R4622:Mycbp2	UTSW	14	103,219,979 (GRCm38)	missense	probably benign	0.12
R4729:Mycbp2	UTSW	14	103,188,591 (GRCm38)	missense	probably damaging	1.00
R4770:Mycbp2	UTSW	14	103,219,944 (GRCm38)	missense	probably benign	0.41
R4790:Mycbp2	UTSW	14	103,229,437 (GRCm38)	missense	probably damaging	1.00
R4884:Mycbp2	UTSW	14	103,211,295 (GRCm38)	missense	probably damaging	1.00
R4885:Mycbp2	UTSW	14	103,145,946 (GRCm38)	missense	possibly damaging	0.86
R4956:Mycbp2	UTSW	14	103,287,239 (GRCm38)	missense	probably damaging	0.99
R4980:Mycbp2	UTSW	14	103,260,385 (GRCm38)	splice site	probably null
R4994:Mycbp2	UTSW	14	103,169,994 (GRCm38)	missense	probably benign
R5029:Mycbp2	UTSW	14	103,156,510 (GRCm38)	missense	probably benign	0.21
R5038:Mycbp2	UTSW	14	103,296,939 (GRCm38)	missense	probably damaging	1.00
R5044:Mycbp2	UTSW	14	103,139,235 (GRCm38)	critical splice donor site	probably null
R5231:Mycbp2	UTSW	14	103,346,214 (GRCm38)	critical splice donor site	probably null
R5305:Mycbp2	UTSW	14	103,346,321 (GRCm38)	missense	probably benign	0.00
R5322:Mycbp2	UTSW	14	103,185,683 (GRCm38)	critical splice acceptor site	probably null
R5376:Mycbp2	UTSW	14	103,242,432 (GRCm38)	nonsense	probably null
R5414:Mycbp2	UTSW	14	103,306,261 (GRCm38)	missense	probably damaging	1.00
R5453:Mycbp2	UTSW	14	103,201,401 (GRCm38)	missense	probably damaging	0.99
R5462:Mycbp2	UTSW	14	103,200,126 (GRCm38)	missense	probably damaging	1.00
R5499:Mycbp2	UTSW	14	103,242,179 (GRCm38)	missense	probably damaging	1.00
R5502:Mycbp2	UTSW	14	103,173,814 (GRCm38)	missense	probably damaging	1.00
R5524:Mycbp2	UTSW	14	103,295,237 (GRCm38)	missense	probably damaging	1.00
R5533:Mycbp2	UTSW	14	103,282,645 (GRCm38)	nonsense	probably null
R5569:Mycbp2	UTSW	14	103,135,243 (GRCm38)	missense	probably damaging	1.00
R5574:Mycbp2	UTSW	14	103,142,767 (GRCm38)	missense	possibly damaging	0.94
R5579:Mycbp2	UTSW	14	103,291,333 (GRCm38)	missense	probably damaging	0.98
R5590:Mycbp2	UTSW	14	103,123,355 (GRCm38)	missense	probably damaging	1.00
R5592:Mycbp2	UTSW	14	103,194,677 (GRCm38)	missense	probably benign	0.02
R5643:Mycbp2	UTSW	14	103,287,334 (GRCm38)	missense	probably damaging	1.00
R5644:Mycbp2	UTSW	14	103,287,334 (GRCm38)	missense	probably damaging	1.00
R5645:Mycbp2	UTSW	14	103,188,615 (GRCm38)	critical splice acceptor site	probably null
R5645:Mycbp2	UTSW	14	103,188,608 (GRCm38)	missense	probably damaging	1.00
R5646:Mycbp2	UTSW	14	103,169,910 (GRCm38)	missense	probably benign	0.09
R5648:Mycbp2	UTSW	14	103,291,342 (GRCm38)	missense	probably damaging	1.00
R5651:Mycbp2	UTSW	14	103,282,665 (GRCm38)	missense	probably null	0.99
R5668:Mycbp2	UTSW	14	103,120,519 (GRCm38)	missense	possibly damaging	0.62
R5745:Mycbp2	UTSW	14	103,156,453 (GRCm38)	missense	possibly damaging	0.94
R5751:Mycbp2	UTSW	14	103,148,550 (GRCm38)	missense	probably damaging	0.99
R5756:Mycbp2	UTSW	14	103,133,974 (GRCm38)	missense	probably damaging	0.99
R5837:Mycbp2	UTSW	14	103,124,403 (GRCm38)	missense	probably damaging	1.00
R5984:Mycbp2	UTSW	14	103,126,684 (GRCm38)	missense	probably damaging	0.98
R6005:Mycbp2	UTSW	14	103,156,723 (GRCm38)	missense	probably benign
R6063:Mycbp2	UTSW	14	103,135,146 (GRCm38)	missense	probably damaging	1.00
R6091:Mycbp2	UTSW	14	103,223,046 (GRCm38)	missense	probably damaging	1.00
R6120:Mycbp2	UTSW	14	103,275,887 (GRCm38)	missense	probably benign	0.01
R6129:Mycbp2	UTSW	14	103,285,400 (GRCm38)	missense	probably benign	0.21
R6147:Mycbp2	UTSW	14	103,155,509 (GRCm38)	nonsense	probably null
R6161:Mycbp2	UTSW	14	103,298,747 (GRCm38)	missense	probably damaging	1.00
R6187:Mycbp2	UTSW	14	103,147,017 (GRCm38)	missense	probably damaging	1.00
R6208:Mycbp2	UTSW	14	103,295,228 (GRCm38)	missense	probably benign	0.11
R6228:Mycbp2	UTSW	14	103,260,229 (GRCm38)	missense	probably benign	0.24
R6301:Mycbp2	UTSW	14	103,155,426 (GRCm38)	missense	probably damaging	1.00
R6311:Mycbp2	UTSW	14	103,262,740 (GRCm38)	missense	possibly damaging	0.93
R6329:Mycbp2	UTSW	14	103,155,852 (GRCm38)	missense	probably benign	0.00
R6439:Mycbp2	UTSW	14	103,155,475 (GRCm38)	missense	probably benign	0.00
R6462:Mycbp2	UTSW	14	103,136,557 (GRCm38)	critical splice donor site	probably null
R6528:Mycbp2	UTSW	14	103,142,881 (GRCm38)	missense	probably damaging	0.99
R6736:Mycbp2	UTSW	14	103,191,567 (GRCm38)	missense	probably null	1.00
R6821:Mycbp2	UTSW	14	103,139,409 (GRCm38)	missense	probably damaging	1.00
R6851:Mycbp2	UTSW	14	103,260,194 (GRCm38)	critical splice donor site	probably null
R6948:Mycbp2	UTSW	14	103,285,267 (GRCm38)	missense	possibly damaging	0.94
R6977:Mycbp2	UTSW	14	103,154,906 (GRCm38)	missense	probably damaging	0.99
R6985:Mycbp2	UTSW	14	103,206,681 (GRCm38)	missense	possibly damaging	0.79
R7035:Mycbp2	UTSW	14	103,174,981 (GRCm38)	missense	probably benign
R7054:Mycbp2	UTSW	14	103,156,098 (GRCm38)	missense	possibly damaging	0.90
R7108:Mycbp2	UTSW	14	103,122,603 (GRCm38)	missense	probably damaging	1.00
R7117:Mycbp2	UTSW	14	103,154,077 (GRCm38)	missense	probably benign	0.21
R7137:Mycbp2	UTSW	14	103,282,679 (GRCm38)	missense	possibly damaging	0.94
R7169:Mycbp2	UTSW	14	103,260,200 (GRCm38)	missense	possibly damaging	0.78
R7218:Mycbp2	UTSW	14	103,133,846 (GRCm38)	missense	probably benign
R7234:Mycbp2	UTSW	14	103,215,337 (GRCm38)	missense	probably damaging	0.98
R7238:Mycbp2	UTSW	14	103,156,297 (GRCm38)	missense	probably damaging	1.00
R7244:Mycbp2	UTSW	14	103,208,909 (GRCm38)	missense	probably damaging	0.98
R7265:Mycbp2	UTSW	14	103,197,243 (GRCm38)	critical splice donor site	probably null
R7286:Mycbp2	UTSW	14	103,120,591 (GRCm38)	missense	probably damaging	1.00
R7332:Mycbp2	UTSW	14	103,197,357 (GRCm38)	missense	probably damaging	0.97
R7332:Mycbp2	UTSW	14	103,156,453 (GRCm38)	missense	probably damaging	1.00
R7384:Mycbp2	UTSW	14	103,276,393 (GRCm38)	missense	probably damaging	0.99
R7392:Mycbp2	UTSW	14	103,243,128 (GRCm38)	missense	probably damaging	0.99
R7392:Mycbp2	UTSW	14	103,152,191 (GRCm38)	missense	probably damaging	1.00
R7409:Mycbp2	UTSW	14	103,288,744 (GRCm38)	missense	probably damaging	1.00
R7486:Mycbp2	UTSW	14	103,197,254 (GRCm38)	missense	probably damaging	0.97
R7643:Mycbp2	UTSW	14	103,346,265 (GRCm38)	missense	probably benign
R7661:Mycbp2	UTSW	14	103,212,623 (GRCm38)	missense	probably damaging	1.00
R7663:Mycbp2	UTSW	14	103,191,609 (GRCm38)	missense	probably damaging	0.99
R7730:Mycbp2	UTSW	14	103,123,355 (GRCm38)	missense	probably damaging	0.99
R7773:Mycbp2	UTSW	14	103,248,404 (GRCm38)	missense	probably damaging	0.97
R7787:Mycbp2	UTSW	14	103,127,097 (GRCm38)	missense	probably damaging	1.00
R7822:Mycbp2	UTSW	14	103,139,415 (GRCm38)	missense	probably benign	0.00
R7838:Mycbp2	UTSW	14	103,177,293 (GRCm38)	missense	probably benign	0.10
R7841:Mycbp2	UTSW	14	103,146,831 (GRCm38)	critical splice donor site	probably null
R7858:Mycbp2	UTSW	14	103,156,305 (GRCm38)	missense	probably damaging	1.00
R7873:Mycbp2	UTSW	14	103,156,146 (GRCm38)	missense	probably damaging	1.00
R7911:Mycbp2	UTSW	14	103,200,185 (GRCm38)	missense	probably damaging	0.99
R7942:Mycbp2	UTSW	14	103,155,238 (GRCm38)	missense	probably damaging	0.99
R7951:Mycbp2	UTSW	14	103,215,462 (GRCm38)	missense	probably damaging	0.99
R7958:Mycbp2	UTSW	14	103,129,964 (GRCm38)	missense	probably benign	0.00
R8235:Mycbp2	UTSW	14	103,198,674 (GRCm38)	missense	probably damaging	0.99
R8246:Mycbp2	UTSW	14	103,155,204 (GRCm38)	missense	probably damaging	0.99
R8338:Mycbp2	UTSW	14	103,135,265 (GRCm38)	missense	probably damaging	1.00
R8343:Mycbp2	UTSW	14	103,160,675 (GRCm38)	splice site	probably null
R8361:Mycbp2	UTSW	14	103,138,814 (GRCm38)	missense	probably damaging	1.00
R8490:Mycbp2	UTSW	14	103,208,831 (GRCm38)	missense	probably benign	0.00
R8524:Mycbp2	UTSW	14	103,155,459 (GRCm38)	missense	probably benign	0.23
R8525:Mycbp2	UTSW	14	103,212,719 (GRCm38)	missense	probably damaging	1.00
R8711:Mycbp2	UTSW	14	103,169,994 (GRCm38)	missense	probably benign	0.08
R8735:Mycbp2	UTSW	14	103,223,150 (GRCm38)	missense	probably damaging	0.99
R8825:Mycbp2	UTSW	14	103,229,435 (GRCm38)	missense	probably damaging	1.00
R8928:Mycbp2	UTSW	14	103,156,345 (GRCm38)	missense	probably benign
R8974:Mycbp2	UTSW	14	103,124,421 (GRCm38)	missense	probably damaging	1.00
R8987:Mycbp2	UTSW	14	103,208,796 (GRCm38)	missense	probably damaging	1.00
R9021:Mycbp2	UTSW	14	103,314,316 (GRCm38)	missense	probably benign	0.08
R9062:Mycbp2	UTSW	14	103,242,360 (GRCm38)	missense	probably benign	0.00
R9077:Mycbp2	UTSW	14	103,232,538 (GRCm38)	missense	probably damaging	1.00
R9208:Mycbp2	UTSW	14	103,295,228 (GRCm38)	missense	probably benign	0.01
R9285:Mycbp2	UTSW	14	103,197,317 (GRCm38)	missense	probably damaging	0.97
R9290:Mycbp2	UTSW	14	103,188,524 (GRCm38)	missense	probably damaging	0.99
R9362:Mycbp2	UTSW	14	103,260,206 (GRCm38)	missense	probably damaging	0.97
R9520:Mycbp2	UTSW	14	103,260,269 (GRCm38)	missense	probably benign	0.02
R9557:Mycbp2	UTSW	14	103,135,261 (GRCm38)	missense	probably benign	0.03
R9639:Mycbp2	UTSW	14	103,196,381 (GRCm38)	missense	probably damaging	1.00
R9666:Mycbp2	UTSW	14	103,134,038 (GRCm38)	missense	probably damaging	0.98
R9732:Mycbp2	UTSW	14	103,211,313 (GRCm38)	missense	probably damaging	1.00
R9736:Mycbp2	UTSW	14	103,197,416 (GRCm38)	missense	probably damaging	0.96
R9739:Mycbp2	UTSW	14	103,282,793 (GRCm38)	missense	probably benign	0.11
R9755:Mycbp2	UTSW	14	103,314,370 (GRCm38)	missense	probably benign
X0024:Mycbp2	UTSW	14	103,146,942 (GRCm38)	missense	probably damaging	1.00
Z1176:Mycbp2	UTSW	14	103,346,249 (GRCm38)	missense	probably benign
Z1176:Mycbp2	UTSW	14	103,156,637 (GRCm38)	missense	probably benign	0.06
Z1177:Mycbp2	UTSW	14	103,135,123 (GRCm38)	missense	probably damaging	1.00
Z1177:Mycbp2	UTSW	14	103,127,063 (GRCm38)	critical splice donor site	probably null
Z1177:Mycbp2	UTSW	14	103,169,873 (GRCm38)	missense	possibly damaging	0.83

Predicted Primers

PCR Primer
(F):5'- TTGTGTCAAGGCCCAAGGATC -3'
(R):5'- ACTTGAATAAGAACTGCTTGCAC -3'

Sequencing Primer
(F):5'- AAGGCCCAAGGATCTTCTTG -3'
(R):5'- AGTAATCCTGGGTCCTGTCAAG -3'

Posted On

2019-11-26