Mutagenetix > Incidental Mutation

Incidental Mutation 'R7762:Prdm15'

597975

Institutional Source

Beutler Lab

Gene Symbol

Prdm15

Ensembl Gene

ENSMUSG00000014039

Gene Name

PR domain containing 15

Synonyms

Zfp298, E130018M06Rik

MMRRC Submission

045818-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7762 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

97592667-97653050 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 97619473 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 318 (I318F)

Ref Sequence

ENSEMBL: ENSMUSP00000113791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095849] [ENSMUST00000121584] [ENSMUST00000142295]

AlphaFold

E9Q8T2

Predicted Effect

probably benign
Transcript: ENSMUST00000095849
AA Change: I344F

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000093533
Gene: ENSMUSG00000014039
AA Change: I344F

Domain	Start	End	E-Value	Type
SET	75	191	5.96e-1	SMART
ZnF_C2H2	223	245	3.99e0	SMART
low complexity region	290	303	N/A	INTRINSIC
ZnF_C2H2	402	424	3.89e-3	SMART
ZnF_C2H2	434	457	2.75e-3	SMART
ZnF_C2H2	468	488	1.88e2	SMART
ZnF_C2H2	495	517	5.42e-2	SMART
ZnF_C2H2	522	544	1.36e-2	SMART
ZnF_C2H2	571	593	6.23e-2	SMART
ZnF_C2H2	598	620	2.75e-3	SMART
low complexity region	642	657	N/A	INTRINSIC
ZnF_C2H2	661	684	2.17e-1	SMART
ZnF_C2H2	689	711	3.24e0	SMART
ZnF_C2H2	725	747	1.38e-3	SMART
ZnF_C2H2	753	775	5.67e-5	SMART
ZnF_C2H2	781	803	3.11e-2	SMART
ZnF_C2H2	809	831	8.34e-3	SMART
ZnF_C2H2	837	859	4.79e-3	SMART
ZnF_C2H2	865	888	4.79e-3	SMART
ZnF_C2H2	894	917	5.06e-2	SMART
low complexity region	948	959	N/A	INTRINSIC
low complexity region	1148	1170	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000121584
AA Change: I318F

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000113791
Gene: ENSMUSG00000014039
AA Change: I318F

Domain	Start	End	E-Value	Type
SET	49	165	5.96e-1	SMART
ZnF_C2H2	197	219	3.99e0	SMART
low complexity region	264	277	N/A	INTRINSIC
ZnF_C2H2	376	398	3.89e-3	SMART
ZnF_C2H2	408	431	2.75e-3	SMART
ZnF_C2H2	442	462	1.88e2	SMART
ZnF_C2H2	469	491	5.42e-2	SMART
ZnF_C2H2	496	518	1.36e-2	SMART
ZnF_C2H2	545	567	6.23e-2	SMART
ZnF_C2H2	572	594	2.75e-3	SMART
low complexity region	616	631	N/A	INTRINSIC
ZnF_C2H2	635	658	2.17e-1	SMART
ZnF_C2H2	663	685	3.24e0	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	5.67e-5	SMART
ZnF_C2H2	755	777	3.11e-2	SMART
ZnF_C2H2	783	805	8.34e-3	SMART
ZnF_C2H2	811	833	4.79e-3	SMART
ZnF_C2H2	839	862	4.79e-3	SMART
ZnF_C2H2	868	891	5.06e-2	SMART
low complexity region	922	933	N/A	INTRINSIC
low complexity region	1122	1144	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000142295
AA Change: I284F

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000120497
Gene: ENSMUSG00000014039
AA Change: I284F

Domain	Start	End	E-Value	Type
SET	49	165	5.96e-1	SMART
low complexity region	230	243	N/A	INTRINSIC
ZnF_C2H2	342	364	3.89e-3	SMART
ZnF_C2H2	369	392	2.75e-3	SMART
ZnF_C2H2	403	423	1.88e2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (79/79)

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acod1	T	G	14: 103,288,776 (GRCm39)	D95E	probably damaging	Het
Agpat4	A	G	17: 12,429,209 (GRCm39)	T154A	possibly damaging	Het
Ahnak2	A	T	12: 112,742,114 (GRCm39)	S653T	probably benign	Het
Aoc1l2	G	A	6: 48,909,620 (GRCm39)	V622I	probably benign	Het
Aox1	C	T	1: 58,388,263 (GRCm39)	P1124S	probably damaging	Het
Armh3	A	G	19: 45,928,882 (GRCm39)		probably null	Het
Atp4a	T	C	7: 30,419,461 (GRCm39)	I637T	probably damaging	Het
Atxn7	T	A	14: 14,100,467 (GRCm38)	C718S	probably damaging	Het
Btbd2	T	A	10: 80,479,390 (GRCm39)	I516F	probably damaging	Het
Card6	A	G	15: 5,134,820 (GRCm39)	S128P	probably benign	Het
Cat	T	C	2: 103,287,203 (GRCm39)	K476E	probably benign	Het
Ccr9	A	T	9: 123,609,022 (GRCm39)	T235S	probably benign	Het
Cep55	T	G	19: 38,057,517 (GRCm39)		probably null	Het
Clec2e	A	G	6: 129,072,091 (GRCm39)	F96S	possibly damaging	Het
Clk2	G	A	3: 89,074,498 (GRCm39)	V53I	probably benign	Het
Clnk	T	C	5: 38,925,484 (GRCm39)	M106V	probably benign	Het
Col6a5	T	C	9: 105,808,523 (GRCm39)	I842V	unknown	Het
Csf1r	A	T	18: 61,243,572 (GRCm39)	N196I	probably benign	Het
D5Ertd579e	A	T	5: 36,770,725 (GRCm39)	N116K		Het
Dnaja4	A	G	9: 54,616,494 (GRCm39)	I166V	probably benign	Het
Dqx1	A	G	6: 83,038,013 (GRCm39)	E467G	probably damaging	Het
Dync2h1	T	C	9: 7,129,719 (GRCm39)	T1760A	probably benign	Het
Eea1	T	C	10: 95,864,301 (GRCm39)	V940A	probably benign	Het
Egr1	T	A	18: 34,996,598 (GRCm39)	V460E	probably damaging	Het
Eral1	A	G	11: 77,965,359 (GRCm39)	I352T	possibly damaging	Het
F2	T	C	2: 91,459,041 (GRCm39)	H476R	possibly damaging	Het
Fam83b	A	G	9: 76,399,714 (GRCm39)	V463A	possibly damaging	Het
Fat1	T	A	8: 45,490,374 (GRCm39)	L3762H	probably damaging	Het
Fat1	T	C	8: 45,476,359 (GRCm39)	S1802P	probably damaging	Het
Fibp	A	T	19: 5,514,202 (GRCm39)	N296Y	probably benign	Het
Figla	A	G	6: 85,994,308 (GRCm39)	M28V	probably benign	Het
Foxd3	C	A	4: 99,545,362 (GRCm39)	Y167*	probably null	Het
Gm17019	T	A	5: 15,081,006 (GRCm39)	H145L	probably benign	Het
Gm48552	C	T	10: 81,226,269 (GRCm39)	P18L	probably damaging	Het
H2-Q6	A	G	17: 35,647,077 (GRCm39)	N283S	probably benign	Het
Hrh2	T	A	13: 54,368,058 (GRCm39)	C11*	probably null	Het
Hrnr	G	T	3: 93,239,506 (GRCm39)	G3248V	unknown	Het
Iapp	C	A	6: 142,249,122 (GRCm39)	N58K	possibly damaging	Het
Itga5	T	C	15: 103,258,184 (GRCm39)	N837S	probably benign	Het
Klhl35	C	A	7: 99,117,647 (GRCm39)	H64N	probably benign	Het
Lcor	T	C	19: 41,572,106 (GRCm39)	L287S	probably benign	Het
Lrba	T	A	3: 86,439,508 (GRCm39)	V2015E	probably damaging	Het
Mdn1	T	C	4: 32,734,421 (GRCm39)	I3276T	probably benign	Het
Mlh3	G	T	12: 85,315,058 (GRCm39)	T376K	possibly damaging	Het
Muc21	G	A	17: 35,932,977 (GRCm39)	T403I	unknown	Het
Nbea	A	T	3: 55,557,126 (GRCm39)	H2550Q	probably damaging	Het
Nid1	G	A	13: 13,663,630 (GRCm39)	G763D	probably damaging	Het
Ntf5	C	A	7: 45,065,243 (GRCm39)	A125E	probably damaging	Het
Obsl1	A	T	1: 75,480,167 (GRCm39)	C460S	probably benign	Het
Or1j19	A	T	2: 36,677,022 (GRCm39)	I162F	probably benign	Het
Or1s2	A	T	19: 13,758,650 (GRCm39)	R223W	probably damaging	Het
Or2g7	G	A	17: 38,378,566 (GRCm39)	C168Y	probably damaging	Het
Or2y10	T	A	11: 49,455,588 (GRCm39)	I280N	possibly damaging	Het
Or4b1b	T	A	2: 90,126,975 (GRCm39)	K77*	probably null	Het
Or6c33	A	G	10: 129,853,050 (GRCm39)		probably benign	Het
Or8d1b	G	A	9: 38,887,490 (GRCm39)	V173I	probably benign	Het
Orai3	G	A	7: 127,372,743 (GRCm39)	G130S	unknown	Het
Pcdhb12	T	G	18: 37,568,977 (GRCm39)	V41G	probably damaging	Het
Plec	A	T	15: 76,067,823 (GRCm39)	L1194Q	unknown	Het
Pml	C	A	9: 58,127,456 (GRCm39)	C763F	probably damaging	Het
Rcbtb2	C	A	14: 73,415,906 (GRCm39)	T473N	probably benign	Het
Sbno1	T	A	5: 124,512,729 (GRCm39)	I1347F	probably benign	Het
Secisbp2l	C	T	2: 125,610,113 (GRCm39)	D269N	probably damaging	Het
Serpina9	A	T	12: 103,967,575 (GRCm39)	F273L	probably damaging	Het
Sgms2	T	A	3: 131,116,898 (GRCm39)	Y319F	probably benign	Het
Sgo2b	A	T	8: 64,379,531 (GRCm39)	H1100Q	probably benign	Het
Sh3bp5l	A	T	11: 58,236,754 (GRCm39)		probably null	Het
Shh	T	G	5: 28,671,664 (GRCm39)	K33T	probably benign	Het
Slc5a9	A	G	4: 111,747,371 (GRCm39)	Y339H	probably damaging	Het
Spice1	T	A	16: 44,190,864 (GRCm39)		probably null	Het
Tbx2	A	G	11: 85,726,727 (GRCm39)	E257G	probably damaging	Het
Tenm2	G	A	11: 35,914,133 (GRCm39)	T2468I	possibly damaging	Het
Tmeff2	A	T	1: 51,018,575 (GRCm39)	N186Y	probably benign	Het
Tmem132c	T	C	5: 127,631,760 (GRCm39)	V673A	possibly damaging	Het
Trappc11	G	T	8: 47,975,411 (GRCm39)	T269K	probably damaging	Het
Trpc6	T	C	9: 8,653,150 (GRCm39)	F652S	possibly damaging	Het
Trpv1	G	T	11: 73,145,048 (GRCm39)	K711N	probably benign	Het
Vcan	G	T	13: 89,841,056 (GRCm39)	P1496Q	probably damaging	Het
Vmn1r192	G	T	13: 22,371,845 (GRCm39)	A125E	probably damaging	Het
Vti1b	A	T	12: 79,211,720 (GRCm39)		probably null	Het
Vwa3b	A	C	1: 37,163,126 (GRCm39)	D583A	probably damaging	Het
Zfp638	T	C	6: 83,953,254 (GRCm39)	S1120P	probably damaging	Het
Zfyve26	A	T	12: 79,315,409 (GRCm39)	F1356I	probably benign	Het

Other mutations in Prdm15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00973:Prdm15	APN	16	97,607,367 (GRCm39)	splice site	probably benign
IGL01325:Prdm15	APN	16	97,607,717 (GRCm39)	missense	probably damaging	1.00
IGL02195:Prdm15	APN	16	97,637,029 (GRCm39)	missense	probably damaging	1.00
IGL02473:Prdm15	APN	16	97,638,805 (GRCm39)	splice site	probably null
IGL02502:Prdm15	APN	16	97,640,539 (GRCm39)	missense	probably damaging	1.00
IGL02604:Prdm15	APN	16	97,623,142 (GRCm39)	missense	probably benign
R0408:Prdm15	UTSW	16	97,636,986 (GRCm39)	missense	possibly damaging	0.92
R0437:Prdm15	UTSW	16	97,613,759 (GRCm39)	missense	probably benign	0.00
R0497:Prdm15	UTSW	16	97,595,534 (GRCm39)	missense	possibly damaging	0.63
R0590:Prdm15	UTSW	16	97,598,961 (GRCm39)	missense	possibly damaging	0.95
R0630:Prdm15	UTSW	16	97,638,907 (GRCm39)	missense	probably null	1.00
R0661:Prdm15	UTSW	16	97,630,882 (GRCm39)	missense	probably benign	0.34
R0718:Prdm15	UTSW	16	97,613,833 (GRCm39)	missense	possibly damaging	0.89
R1144:Prdm15	UTSW	16	97,609,908 (GRCm39)	missense	probably damaging	1.00
R1240:Prdm15	UTSW	16	97,638,800 (GRCm39)	missense	probably damaging	0.98
R1605:Prdm15	UTSW	16	97,640,506 (GRCm39)	missense	probably damaging	1.00
R1908:Prdm15	UTSW	16	97,638,885 (GRCm39)	missense	probably benign	0.27
R2081:Prdm15	UTSW	16	97,604,980 (GRCm39)	nonsense	probably null
R2208:Prdm15	UTSW	16	97,600,464 (GRCm39)	splice site	probably null
R3787:Prdm15	UTSW	16	97,598,945 (GRCm39)	missense	probably benign	0.00
R3890:Prdm15	UTSW	16	97,600,771 (GRCm39)	missense	probably damaging	1.00
R4326:Prdm15	UTSW	16	97,607,715 (GRCm39)	missense	probably damaging	1.00
R4728:Prdm15	UTSW	16	97,622,986 (GRCm39)	missense	probably benign	0.04
R4952:Prdm15	UTSW	16	97,607,277 (GRCm39)	missense	probably damaging	0.99
R4998:Prdm15	UTSW	16	97,595,689 (GRCm39)	missense	probably damaging	0.97
R5225:Prdm15	UTSW	16	97,609,875 (GRCm39)	missense	probably damaging	1.00
R5505:Prdm15	UTSW	16	97,618,183 (GRCm39)	missense	possibly damaging	0.76
R5628:Prdm15	UTSW	16	97,600,823 (GRCm39)	missense	probably damaging	0.98
R5721:Prdm15	UTSW	16	97,608,296 (GRCm39)	missense	possibly damaging	0.74
R5873:Prdm15	UTSW	16	97,609,889 (GRCm39)	missense	probably damaging	1.00
R5980:Prdm15	UTSW	16	97,613,770 (GRCm39)	nonsense	probably null
R6311:Prdm15	UTSW	16	97,600,255 (GRCm39)	missense	probably null	0.08
R6540:Prdm15	UTSW	16	97,637,005 (GRCm39)	missense	probably benign	0.13
R7053:Prdm15	UTSW	16	97,595,742 (GRCm39)	nonsense	probably null
R7241:Prdm15	UTSW	16	97,596,941 (GRCm39)	missense	possibly damaging	0.50
R7468:Prdm15	UTSW	16	97,636,842 (GRCm39)	nonsense	probably null
R7473:Prdm15	UTSW	16	97,623,046 (GRCm39)	missense	possibly damaging	0.68
R7911:Prdm15	UTSW	16	97,613,792 (GRCm39)	missense	probably benign	0.35
R8053:Prdm15	UTSW	16	97,636,807 (GRCm39)	missense	probably benign	0.17
R8127:Prdm15	UTSW	16	97,638,910 (GRCm39)	missense	probably benign	0.24
R8213:Prdm15	UTSW	16	97,608,260 (GRCm39)	missense	probably damaging	1.00
R8708:Prdm15	UTSW	16	97,618,066 (GRCm39)	missense	unknown
R8768:Prdm15	UTSW	16	97,638,888 (GRCm39)	missense	probably benign
R9000:Prdm15	UTSW	16	97,595,470 (GRCm39)	missense	probably benign	0.03
R9513:Prdm15	UTSW	16	97,607,704 (GRCm39)	missense	probably damaging	1.00
R9583:Prdm15	UTSW	16	97,623,142 (GRCm39)	missense	probably benign
RF002:Prdm15	UTSW	16	97,600,829 (GRCm39)	missense	probably damaging	1.00
RF021:Prdm15	UTSW	16	97,609,956 (GRCm39)	missense	probably damaging	1.00
Z1177:Prdm15	UTSW	16	97,618,159 (GRCm39)	missense	possibly damaging	0.54

Predicted Primers

PCR Primer
(F):5'- AACATACAGTGCTGGCCAG -3'
(R):5'- AACATGTTCGACTTGTATCCCTG -3'

Sequencing Primer
(F):5'- CATACAGTGCTGGCCAGAAGAG -3'
(R):5'- TGTTGTATCCACACCCAGGAG -3'

Posted On

2019-11-26