Mutagenetix > Incidental Mutation

Incidental Mutation 'R7763:Ralgapa1'

598055

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, Tulip1, 2310003F20Rik

MMRRC Submission

045819-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.802) question?

Stock #

R7763 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

55649681-55867952 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 55804740 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 519 (I519F)

Ref Sequence

ENSEMBL: ENSMUSP00000082503 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably benign
Transcript: ENSMUST00000085385
AA Change: I519F

PolyPhen 2 Score 0.281 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: I519F

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000110687
AA Change: I519F

PolyPhen 2 Score 0.281 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: I519F

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000219432
AA Change: I519F

PolyPhen 2 Score 0.763 (Sensitivity: 0.85; Specificity: 0.92)

Predicted Effect

probably benign
Transcript: ENSMUST00000220367
AA Change: I519F

PolyPhen 2 Score 0.403 (Sensitivity: 0.89; Specificity: 0.89)

Predicted Effect

probably damaging
Transcript: ENSMUST00000226244
AA Change: I519F

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 111 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	T	A	7: 120,113,825 (GRCm39)	W899R	probably damaging	Het
Abca5	A	T	11: 110,163,323 (GRCm39)	W1631R	possibly damaging	Het
Actg2	T	A	6: 83,504,350 (GRCm39)	D25V	probably damaging	Het
Akap10	G	C	11: 61,806,331 (GRCm39)	D132E	probably damaging	Het
Angptl2	G	T	2: 33,132,394 (GRCm39)	E334*	probably null	Het
Aprt	T	C	8: 123,301,674 (GRCm39)	R165G	probably benign	Het
Bmp1	A	G	14: 70,729,524 (GRCm39)	F549S	probably damaging	Het
Capzb	C	T	4: 139,007,864 (GRCm39)	T215I	probably benign	Het
Car14	C	T	3: 95,811,684 (GRCm39)	M1I	probably null	Het
Ccdc148	T	A	2: 58,713,648 (GRCm39)	Q501L	probably benign	Het
Ccdc32	G	A	2: 118,857,828 (GRCm39)	T12I	probably damaging	Het
Ccnf	G	A	17: 24,443,986 (GRCm39)	S594L	probably damaging	Het
Cdadc1	C	T	14: 59,811,283 (GRCm39)	C409Y	probably damaging	Het
Cdh23	G	T	10: 60,148,356 (GRCm39)	S2670R	probably damaging	Het
Cdh8	T	A	8: 100,006,306 (GRCm39)	K94*	probably null	Het
Cdhr2	A	T	13: 54,865,505 (GRCm39)	Y193F	probably damaging	Het
Cdon	T	A	9: 35,365,711 (GRCm39)	Y153*	probably null	Het
Chd1	G	A	17: 15,953,303 (GRCm39)	G413R	probably damaging	Het
Cit	A	T	5: 116,125,060 (GRCm39)	T1582S	probably benign	Het
Clec4a3	T	C	6: 122,941,299 (GRCm39)	L98P	probably benign	Het
Cpa4	T	A	6: 30,583,644 (GRCm39)	D253E	probably damaging	Het
Cyp2c40	T	A	19: 39,795,612 (GRCm39)	N189I	possibly damaging	Het
Daam2	G	T	17: 49,797,050 (GRCm39)	A245E	probably benign	Het
Dennd5b	A	G	6: 148,970,156 (GRCm39)	F121S	probably damaging	Het
Dgkd	A	G	1: 87,854,671 (GRCm39)	T658A	probably benign	Het
Dmwd	T	A	7: 18,814,265 (GRCm39)	L305Q	probably damaging	Het
Dnah5	A	T	15: 28,314,001 (GRCm39)	Y1939F	probably damaging	Het
Dock5	T	C	14: 68,058,776 (GRCm39)	T512A	probably damaging	Het
Dop1b	T	A	16: 93,552,402 (GRCm39)	D398E	probably benign	Het
Eef1akmt4	A	G	16: 20,437,279 (GRCm39)	H207R	probably damaging	Het
Egfr	T	C	11: 16,841,266 (GRCm39)	V719A	probably damaging	Het
Ep300	A	T	15: 81,470,784 (GRCm39)		probably benign	Het
Epha4	A	G	1: 77,366,668 (GRCm39)		probably null	Het
Erc2	T	G	14: 27,598,161 (GRCm39)		probably null	Het
Ergic1	A	T	17: 26,857,801 (GRCm39)	Y209F	possibly damaging	Het
Fbxw28	A	G	9: 109,155,701 (GRCm39)	I357T	probably damaging	Het
Flnb	A	T	14: 7,926,478 (GRCm38)	T1841S	probably benign	Het
Foxc1	G	A	13: 31,992,011 (GRCm39)	S274N	probably benign	Het
Fto	C	T	8: 92,136,071 (GRCm39)	T115M	probably damaging	Het
Gm10228	C	G	16: 88,838,187 (GRCm39)	C39S	unknown	Het
Gm7334	T	C	17: 51,005,743 (GRCm39)	F10L	possibly damaging	Het
Hfm1	A	G	5: 107,029,727 (GRCm39)	Y785H	probably damaging	Het
Hivep1	T	A	13: 42,312,937 (GRCm39)	S1726T	probably benign	Het
Htt	G	T	5: 35,009,534 (GRCm39)	C1505F	probably damaging	Het
Hydin	T	C	8: 111,232,475 (GRCm39)	S1665P	possibly damaging	Het
Ifi209	T	A	1: 173,470,445 (GRCm39)	N344K	probably damaging	Het
Ifnar2	T	C	16: 91,196,181 (GRCm39)	M262T	probably benign	Het
Ighv7-3	T	A	12: 114,116,827 (GRCm39)	S112C	probably damaging	Het
Igkv4-86	A	G	6: 68,887,563 (GRCm39)	S59P	probably benign	Het
Iglv1	A	G	16: 18,904,239 (GRCm39)		probably benign	Het
Ipo7	T	A	7: 109,652,006 (GRCm39)	D928E	possibly damaging	Het
Itgae	G	T	11: 73,014,095 (GRCm39)		probably null	Het
Kbtbd12	T	A	6: 88,595,179 (GRCm39)	Q217L	probably benign	Het
Kcnma1	T	A	14: 23,350,074 (GRCm39)	Y1155F	possibly damaging	Het
Kif14	A	G	1: 136,444,121 (GRCm39)	E1371G	probably benign	Het
Lilrb4a	A	T	10: 51,367,142 (GRCm39)	Y10F	probably benign	Het
Lrp2	A	T	2: 69,333,732 (GRCm39)	V1503E	probably damaging	Het
Mfsd6	A	T	1: 52,747,799 (GRCm39)	D355E	probably benign	Het
Mri1	T	C	8: 84,977,657 (GRCm39)	H226R		Het
Mroh4	C	T	15: 74,496,554 (GRCm39)	E278K	probably damaging	Het
Muc4	T	A	16: 32,574,739 (GRCm39)	L1063H	probably benign	Het
Mzf1	A	T	7: 12,778,018 (GRCm39)	I541N	probably damaging	Het
Nasp	T	A	4: 116,469,230 (GRCm39)	E116D	probably benign	Het
Nlrp10	T	A	7: 108,525,033 (GRCm39)	E149V	probably damaging	Het
Notch4	G	A	17: 34,801,392 (GRCm39)	C1080Y	probably damaging	Het
Nova1	T	G	12: 46,767,481 (GRCm39)	I147L	unknown	Het
Ogdh	A	G	11: 6,288,558 (GRCm39)	M223V	probably benign	Het
Or2a56	T	C	6: 42,932,723 (GRCm39)	I97T	probably benign	Het
Or2y1e	G	T	11: 49,218,498 (GRCm39)	A87S	probably benign	Het
Or4n4	A	T	14: 50,518,945 (GRCm39)	I255N	probably damaging	Het
Or6c66b	G	T	10: 129,377,324 (GRCm39)	R306L	probably benign	Het
P3h3	T	C	6: 124,831,395 (GRCm39)	Q330R	probably benign	Het
Pcdhb10	A	G	18: 37,544,935 (GRCm39)	T4A	not run	Het
Pkd2l2	A	T	18: 34,566,340 (GRCm39)		probably null	Het
Plekhb1	C	T	7: 100,294,870 (GRCm39)	V168I	probably benign	Het
Plxna4	C	T	6: 32,200,915 (GRCm39)	R753H	probably damaging	Het
Pramel17	T	C	4: 101,694,338 (GRCm39)	I182V	possibly damaging	Het
Prex1	G	C	2: 166,555,629 (GRCm39)	P4A	unknown	Het
Ptgdr	A	G	14: 45,096,535 (GRCm39)	V59A	probably damaging	Het
Rbpms	A	G	8: 34,279,481 (GRCm39)	I170T	probably benign	Het
Sec31b	T	A	19: 44,512,274 (GRCm39)		probably null	Het
Skint11	A	T	4: 114,084,905 (GRCm39)	Y138F	probably benign	Het
Slain2	A	G	5: 73,105,953 (GRCm39)	Y196C	probably damaging	Het
Slco1b2	T	A	6: 141,621,950 (GRCm39)	C503*	probably null	Het
Slfn3	A	T	11: 83,105,614 (GRCm39)	Y537F	possibly damaging	Het
Slu7	T	C	11: 43,335,592 (GRCm39)	Y443H	probably damaging	Het
Sorl1	T	A	9: 41,955,205 (GRCm39)	E683D	probably damaging	Het
Sos1	T	A	17: 80,721,142 (GRCm39)	I893L	probably benign	Het
St8sia2	T	A	7: 73,593,069 (GRCm39)	Y329F	probably damaging	Het
Stra6l	C	A	4: 45,869,570 (GRCm39)	S212*	probably null	Het
Syne3	C	T	12: 104,963,754 (GRCm39)		probably benign	Het
Tenm4	T	A	7: 96,544,899 (GRCm39)	I2342N	probably benign	Het
Tescl	T	A	7: 24,032,688 (GRCm39)	E212D	probably benign	Het
Trip11	A	G	12: 101,811,114 (GRCm39)	S1879P	probably benign	Het
Ube2c	A	T	2: 164,613,211 (GRCm39)		probably null	Het
Umodl1	T	A	17: 31,205,430 (GRCm39)	I675N	probably benign	Het
Vipr2	T	C	12: 116,086,338 (GRCm39)	F121S	probably damaging	Het
Vmn1r206	A	T	13: 22,804,839 (GRCm39)	S123T	possibly damaging	Het
Vmn1r58	A	G	7: 5,413,912 (GRCm39)	V106A	probably damaging	Het
Vmn2r112	A	G	17: 22,822,099 (GRCm39)	Y259C	probably damaging	Het
Vmn2r66	T	C	7: 84,654,909 (GRCm39)	K467E	probably benign	Het
Vmn2r98	T	C	17: 19,300,797 (GRCm39)	F600L	probably benign	Het
Vps13a	T	A	19: 16,723,364 (GRCm39)	N278I	possibly damaging	Het
Vwa5a	A	C	9: 38,652,458 (GRCm39)	D747A	possibly damaging	Het
Xdh	G	A	17: 74,241,829 (GRCm39)	P157S	possibly damaging	Het
Xrn1	G	A	9: 95,880,401 (GRCm39)		probably null	Het
Zfp362	T	A	4: 128,680,824 (GRCm39)	H180L	probably benign	Het
Zfp560	A	T	9: 20,258,619 (GRCm39)	W748R	possibly damaging	Het
Zfp808	A	T	13: 62,320,478 (GRCm39)	Q569L	probably benign	Het
Zfp93	T	A	7: 23,974,643 (GRCm39)	D209E	possibly damaging	Het
Zftraf1	A	T	15: 76,542,747 (GRCm39)	Y138N	probably damaging	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,769,558 (GRCm39)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,793,970 (GRCm39)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,749,237 (GRCm39)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,756,360 (GRCm39)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,689,144 (GRCm39)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,689,133 (GRCm39)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,824,101 (GRCm39)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,766,442 (GRCm39)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,689,262 (GRCm39)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,754,862 (GRCm39)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,689,234 (GRCm39)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,759,450 (GRCm39)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,720,292 (GRCm39)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,723,202 (GRCm39)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,763,854 (GRCm39)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,804,736 (GRCm39)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
bantam	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
wickedwarlock	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,842,438 (GRCm39)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,741,942 (GRCm39)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,833,048 (GRCm39)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,786,290 (GRCm39)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,723,354 (GRCm39)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,723,823 (GRCm39)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,754,852 (GRCm39)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,736,576 (GRCm39)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,723,550 (GRCm39)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,829,670 (GRCm39)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,842,483 (GRCm39)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,712,448 (GRCm39)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,723,366 (GRCm39)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,809,446 (GRCm39)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,754,763 (GRCm39)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,723,711 (GRCm39)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,788,265 (GRCm39)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,731,309 (GRCm39)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,817,488 (GRCm39)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,788,321 (GRCm39)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,809,388 (GRCm39)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,723,552 (GRCm39)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,689,174 (GRCm39)	missense	probably damaging	1.00
R1832:Ralgapa1	UTSW	12	55,804,752 (GRCm39)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,723,817 (GRCm39)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,833,107 (GRCm39)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,723,811 (GRCm39)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,741,945 (GRCm39)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,822,973 (GRCm39)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,723,909 (GRCm39)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,867,540 (GRCm39)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,705,922 (GRCm39)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,817,398 (GRCm39)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,741,928 (GRCm39)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,705,915 (GRCm39)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,745,552 (GRCm39)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,842,486 (GRCm39)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,786,115 (GRCm39)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,724,061 (GRCm39)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,740,243 (GRCm39)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,759,533 (GRCm39)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,841,778 (GRCm39)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,723,222 (GRCm39)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,745,588 (GRCm39)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,809,359 (GRCm39)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,764,899 (GRCm39)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,659,508 (GRCm39)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,712,459 (GRCm39)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,822,937 (GRCm39)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,804,817 (GRCm39)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,723,582 (GRCm39)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,766,408 (GRCm39)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,723,495 (GRCm39)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,659,523 (GRCm39)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,867,551 (GRCm39)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,723,898 (GRCm39)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,785,050 (GRCm39)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,817,401 (GRCm39)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,730,827 (GRCm39)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,804,709 (GRCm39)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,793,931 (GRCm39)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,730,695 (GRCm39)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,766,446 (GRCm39)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,785,104 (GRCm39)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,809,512 (GRCm39)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,651,058 (GRCm39)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,832,997 (GRCm39)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,822,976 (GRCm39)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,804,844 (GRCm39)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,867,508 (GRCm39)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,768,361 (GRCm39)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,650,976 (GRCm39)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,741,978 (GRCm39)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,755,789 (GRCm39)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,759,457 (GRCm39)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,765,013 (GRCm39)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,705,928 (GRCm39)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,756,341 (GRCm39)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,756,340 (GRCm39)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,788,298 (GRCm39)	missense	probably benign
R7791:Ralgapa1	UTSW	12	55,788,304 (GRCm39)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,766,413 (GRCm39)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,659,423 (GRCm39)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,793,934 (GRCm39)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,744,663 (GRCm39)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,749,242 (GRCm39)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,829,631 (GRCm39)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,769,699 (GRCm39)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,788,308 (GRCm39)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,705,847 (GRCm39)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,723,303 (GRCm39)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,786,198 (GRCm39)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,785,002 (GRCm39)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,785,101 (GRCm39)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,749,345 (GRCm39)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,867,546 (GRCm39)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,723,791 (GRCm39)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,652,314 (GRCm39)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,723,351 (GRCm39)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,781,881 (GRCm39)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,755,843 (GRCm39)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,754,808 (GRCm39)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,659,485 (GRCm39)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,755,865 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGAATCCCAAAGTATAGGCATTTG -3'
(R):5'- CTTTATGCTTACTGACTCATTGCAG -3'

Sequencing Primer
(F):5'- CATTTTCTCTTGAAAGGTGGCACAC -3'
(R):5'- GACTCATTGCAGCATTCTCATTGATG -3'

Posted On

2019-11-26