Mutagenetix > Incidental Mutation

Incidental Mutation 'R7764:Or9i14'

598156

Institutional Source

Beutler Lab

Gene Symbol

Or9i14

Ensembl Gene

ENSMUSG00000045395

Gene Name

olfactory receptor family 9 subfamily I member 14

Synonyms

GA_x6K02T2RE5P-4147744-4146800, Olfr1499, MOR211-2

MMRRC Submission

045820-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.276) question?

Stock #

R7764 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

13792008-13792952 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 13792111 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 281 (I281T)

Ref Sequence

ENSEMBL: ENSMUSP00000150330 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055672] [ENSMUST00000216659]

AlphaFold

Q8VG65

Predicted Effect

probably benign
Transcript: ENSMUST00000055672
AA Change: I281T

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000059747
Gene: ENSMUSG00000045395
AA Change: I281T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	2.6e-44	PFAM
Pfam:7tm_1	41	290	1.5e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216659
AA Change: I281T

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf3	T	G	16: 20,368,040 (GRCm39)	L93V	probably benign	Het
Adss2	A	C	1: 177,591,827 (GRCm39)	M452R	probably damaging	Het
Aldh3b1	T	A	19: 3,971,563 (GRCm39)	K102*	probably null	Het
Ccdc162	A	T	10: 41,566,109 (GRCm39)	V78D	possibly damaging	Het
Ccr3	T	C	9: 123,829,451 (GRCm39)	V262A	probably benign	Het
Cdk13	A	T	13: 17,895,890 (GRCm39)		probably null	Het
Cdyl	A	T	13: 36,000,126 (GRCm39)	T136S	possibly damaging	Het
Chd2	T	C	7: 73,121,567 (GRCm39)	D1015G	probably null	Het
Cntn2	C	A	1: 132,450,101 (GRCm39)	A598S	probably benign	Het
Cxxc4	G	T	3: 133,945,856 (GRCm39)	G146C	unknown	Het
Dnah2	G	A	11: 69,348,984 (GRCm39)	T2501I	probably benign	Het
Dock8	T	A	19: 25,074,899 (GRCm39)	I471N	probably benign	Het
Eaf2	A	G	16: 36,645,045 (GRCm39)	V59A	probably damaging	Het
Egflam	T	A	15: 7,347,736 (GRCm39)	I65F	probably damaging	Het
Exoc3l	A	G	8: 106,017,333 (GRCm39)	V577A	possibly damaging	Het
Folh1	A	T	7: 86,412,126 (GRCm39)	M215K	probably benign	Het
Fosb	T	C	7: 19,038,971 (GRCm39)	D271G	possibly damaging	Het
Frmd4b	A	G	6: 97,272,891 (GRCm39)	Y834H	probably damaging	Het
Fsip2	T	C	2: 82,811,252 (GRCm39)	S2524P	possibly damaging	Het
Gbp3	A	G	3: 142,271,024 (GRCm39)	T143A	probably benign	Het
Grk2	A	T	19: 4,337,391 (GRCm39)	L632Q	probably damaging	Het
Hars1	A	T	18: 36,903,237 (GRCm39)	D364E	probably damaging	Het
Hsd17b4	G	T	18: 50,279,482 (GRCm39)	G154*	probably null	Het
Kif5c	T	C	2: 49,617,973 (GRCm39)		probably null	Het
Kif5c	T	C	2: 49,639,339 (GRCm39)	L800P	probably damaging	Het
Krt19	T	C	11: 100,032,218 (GRCm39)	N282S	probably benign	Het
Med23	T	G	10: 24,785,818 (GRCm39)		probably null	Het
Meltf	A	G	16: 31,699,085 (GRCm39)	Q65R	probably benign	Het
Mms22l	T	C	4: 24,598,842 (GRCm39)	S1106P	probably damaging	Het
Mprip	A	G	11: 59,655,242 (GRCm39)	T733A	probably damaging	Het
Mul1	G	A	4: 138,162,080 (GRCm39)	G4S	possibly damaging	Het
Myl10	G	C	5: 136,726,825 (GRCm39)	V70L	probably benign	Het
Mylk	G	T	16: 34,742,553 (GRCm39)	V1022L	probably benign	Het
Ncald	G	T	15: 37,397,454 (GRCm39)	N75K	probably damaging	Het
Nfib	A	G	4: 82,238,731 (GRCm39)	S492P	possibly damaging	Het
Notch2	A	T	3: 98,050,304 (GRCm39)	I1860F	probably damaging	Het
Oxr1	T	C	15: 41,683,263 (GRCm39)	S298P	probably benign	Het
Pcnt	T	C	10: 76,190,082 (GRCm39)	D2818G	probably benign	Het
Pmpcb	G	A	5: 21,948,450 (GRCm39)	A244T	probably damaging	Het
Poln	A	G	5: 34,274,151 (GRCm39)		probably null	Het
Polr1a	G	A	6: 71,930,054 (GRCm39)	V914M	probably damaging	Het
Rab15	A	C	12: 76,851,215 (GRCm39)		probably null	Het
Rasgrf1	G	T	9: 89,876,747 (GRCm39)	S704I	possibly damaging	Het
Rsf1	GGCGGCGG	GGCGGCGGGCGCGGCGG	7: 97,229,134 (GRCm39)		probably benign	Het
Sart1	C	A	19: 5,438,613 (GRCm39)	G15W	probably damaging	Het
Scrib	T	C	15: 75,919,242 (GRCm39)	*1666W	probably null	Het
Setd1b	G	C	5: 123,284,622 (GRCm39)	R184P	unknown	Het
Sfxn2	A	T	19: 46,574,179 (GRCm39)	N123I	probably damaging	Het
Slc22a3	A	G	17: 12,677,383 (GRCm39)	W262R	probably damaging	Het
Slc38a10	A	G	11: 119,995,905 (GRCm39)	L1064P	probably damaging	Het
Sorcs2	A	T	5: 36,181,416 (GRCm39)	S1077R	possibly damaging	Het
Them7	G	T	2: 105,128,171 (GRCm39)	E51*	probably null	Het
Ubc	A	G	5: 125,465,133 (GRCm39)	S65P	possibly damaging	Het
Ubqln3	A	T	7: 103,790,443 (GRCm39)	M549K	possibly damaging	Het
Vmn1r222	A	G	13: 23,416,529 (GRCm39)	L228P	probably damaging	Het
Vmn2r60	A	G	7: 41,844,535 (GRCm39)	T633A	probably damaging	Het
Zfp382	T	C	7: 29,832,700 (GRCm39)	V117A	probably benign	Het
Zic1	C	T	9: 91,247,745 (GRCm39)		probably benign	Het
Zmym4	A	T	4: 126,819,409 (GRCm39)	F165I	probably benign	Het

Other mutations in Or9i14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01534:Or9i14	APN	19	13,792,666 (GRCm39)	missense	probably benign	0.09
IGL01844:Or9i14	APN	19	13,792,180 (GRCm39)	missense	possibly damaging	0.95
IGL03102:Or9i14	APN	19	13,792,735 (GRCm39)	missense	probably damaging	1.00
IGL03352:Or9i14	APN	19	13,792,292 (GRCm39)	missense	probably damaging	1.00
R0218:Or9i14	UTSW	19	13,792,342 (GRCm39)	missense	probably benign	0.19
R0490:Or9i14	UTSW	19	13,792,219 (GRCm39)	missense	probably damaging	1.00
R0682:Or9i14	UTSW	19	13,792,501 (GRCm39)	missense	possibly damaging	0.94
R1301:Or9i14	UTSW	19	13,792,726 (GRCm39)	missense	probably damaging	1.00
R1328:Or9i14	UTSW	19	13,792,900 (GRCm39)	missense	probably benign	0.01
R2100:Or9i14	UTSW	19	13,792,600 (GRCm39)	missense	possibly damaging	0.95
R3701:Or9i14	UTSW	19	13,792,712 (GRCm39)	missense	probably benign	0.03
R4563:Or9i14	UTSW	19	13,792,646 (GRCm39)	missense	probably benign	0.01
R4709:Or9i14	UTSW	19	13,792,814 (GRCm39)	missense	possibly damaging	0.94
R5231:Or9i14	UTSW	19	13,792,711 (GRCm39)	missense	probably damaging	0.99
R5301:Or9i14	UTSW	19	13,792,933 (GRCm39)	missense	probably damaging	0.99
R5343:Or9i14	UTSW	19	13,792,324 (GRCm39)	missense	probably damaging	1.00
R6268:Or9i14	UTSW	19	13,792,671 (GRCm39)	nonsense	probably null
R6442:Or9i14	UTSW	19	13,792,992 (GRCm39)	start gained	probably benign
R7132:Or9i14	UTSW	19	13,792,786 (GRCm39)	missense	probably benign	0.09
R7943:Or9i14	UTSW	19	13,792,600 (GRCm39)	missense	probably damaging	0.99
R8703:Or9i14	UTSW	19	13,792,105 (GRCm39)	missense	probably damaging	1.00
R8736:Or9i14	UTSW	19	13,792,358 (GRCm39)	missense	probably benign	0.05
R9069:Or9i14	UTSW	19	13,792,735 (GRCm39)	missense	probably damaging	1.00
R9177:Or9i14	UTSW	19	13,792,388 (GRCm39)	missense	probably damaging	1.00
R9258:Or9i14	UTSW	19	13,792,099 (GRCm39)	nonsense	probably null
R9268:Or9i14	UTSW	19	13,792,388 (GRCm39)	missense	probably damaging	1.00
Z1088:Or9i14	UTSW	19	13,792,912 (GRCm39)	missense	probably damaging	1.00
Z1177:Or9i14	UTSW	19	13,792,408 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTGGAAGCATTCTTTCTGTTCATAG -3'
(R):5'- ACTTTGTCATCTTGGTGAATGC -3'

Sequencing Primer
(F):5'- TAACGGAGAGGTCATCTC -3'
(R):5'- CCTGCTTATCATCAAGACAGTCATG -3'

Posted On

2019-11-26