Mutagenetix > Incidental Mutations

Incidental Mutation 'R7765:Klf17'

598174

Institutional Source

Beutler Lab

Gene Symbol

Klf17

Ensembl Gene

ENSMUSG00000048626

Gene Name

Kruppel-like factor 17

Synonyms

D4Ertd561e, 7420700M05Rik, Zfp393

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7765 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

117757836-117765648 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 117760615 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 182 (M182L)

Ref Sequence

ENSEMBL: ENSMUSP00000052316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062747]

AlphaFold

Q8CFA7

Predicted Effect

probably benign
Transcript: ENSMUST00000062747
AA Change: M182L

PolyPhen 2 Score 0.083 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000052316
Gene: ENSMUSG00000048626
AA Change: M182L

Domain	Start	End	E-Value	Type
low complexity region	21	34	N/A	INTRINSIC
low complexity region	142	157	N/A	INTRINSIC
low complexity region	202	213	N/A	INTRINSIC
ZnF_C2H2	256	280	4.47e-3	SMART
ZnF_C2H2	286	310	1.92e-2	SMART
ZnF_C2H2	316	338	1.1e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (62/62)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933416C03Rik	T	A	10: 116,113,253	K123*	probably null	Het
Adam2	T	C	14: 66,059,896	E187G	probably damaging	Het
Adam28	C	T	14: 68,609,106		probably null	Het
Ap2a1	G	A	7: 44,909,736	T189M	probably damaging	Het
Apaf1	A	G	10: 91,023,782	Y845H	probably benign	Het
Asb14	G	T	14: 26,897,761	V55F	probably benign	Het
Brap	A	T	5: 121,662,129	D71V	probably damaging	Het
Ccdc93	T	G	1: 121,499,313	F610V	probably damaging	Het
Cfap46	T	G	7: 139,651,564	D911A		Het
Chn2	T	C	6: 54,298,152		probably null	Het
Cib3	A	G	8: 72,204,425	F156S	probably damaging	Het
Ctif	A	T	18: 75,605,644	V164D	probably damaging	Het
Ddx27	T	A	2: 167,027,959	F405I	probably damaging	Het
Dnttip2	A	G	3: 122,275,945	T270A	probably benign	Het
Dst	T	A	1: 34,275,694	S4455T	probably damaging	Het
Efcab8	A	G	2: 153,843,190	K47R		Het
Efhd2	T	C	4: 141,874,575	E98G	probably damaging	Het
Fcmr	T	A	1: 130,874,288	L93Q	probably damaging	Het
Fhdc1	T	A	3: 84,444,599	E1106D	probably benign	Het
Gcnt1	C	T	19: 17,329,359	G334D	probably damaging	Het
Gm10031	G	A	1: 156,524,784	G185D	possibly damaging	Het
Hltf	T	C	3: 20,091,483	F488L	probably benign	Het
Hmgxb4	C	A	8: 75,000,808	H140N	probably damaging	Het
Ifi214	A	T	1: 173,524,836	F342L	probably damaging	Het
Ip6k1	A	G	9: 108,032,089	D105G	possibly damaging	Het
Kank1	GCGAACG	GCG	19: 25,411,205		probably null	Het
Kif18a	G	A	2: 109,306,940	D506N	probably benign	Het
Kmt2d	A	G	15: 98,852,334	F2493L	unknown	Het
Lefty1	A	G	1: 180,936,547	E84G	probably damaging	Het
Lyst	T	G	13: 13,709,532	L2975R	possibly damaging	Het
Magea5	G	A	X: 155,054,178	P73S	possibly damaging	Het
Mctp2	T	C	7: 72,090,331		probably null	Het
Mprip	C	T	11: 59,758,221	T917M	possibly damaging	Het
Myh8	T	A	11: 67,303,655	I1564N	probably benign	Het
Npc1	A	T	18: 12,195,048	M1068K	probably benign	Het
Nr2e1	A	G	10: 42,574,437	C60R	probably benign	Het
Nrxn3	A	G	12: 89,813,484	I29V	probably benign	Het
Ntsr1	T	A	2: 180,538,817	H305Q	probably damaging	Het
Olfr1100	T	A	2: 86,978,194	I201F	probably damaging	Het
Olfr1391	A	G	11: 49,327,744	E111G	probably damaging	Het
Olfr955	A	G	9: 39,470,316	S137P	probably benign	Het
Pate2	A	C	9: 35,669,901	E22D	probably benign	Het
Pcdhb22	A	G	18: 37,519,105	T209A	probably damaging	Het
Pdss2	C	T	10: 43,464,632	S352F	probably benign	Het
Pfn3	T	A	13: 55,415,087	D27V	probably damaging	Het
Pigg	T	G	5: 108,314,054	S84A	probably benign	Het
Prm3	CTCTTCTTCTTCTTC	CTCTTCTTCTTC	16: 10,790,701		probably benign	Het
Prss22	C	T	17: 23,994,618	G233E	probably damaging	Het
Rad51b	T	C	12: 79,803,270		probably null	Het
Scn10a	A	G	9: 119,609,904	V1632A	possibly damaging	Het
Serpinb3d	T	G	1: 107,079,782	D158A	probably damaging	Het
Sh3yl1	T	C	12: 30,958,869	L266P	probably damaging	Het
Sidt2	A	T	9: 45,941,575		probably null	Het
Stox1	C	T	10: 62,665,999	V261M	probably benign	Het
Tll1	A	G	8: 64,051,449	Y638H	probably damaging	Het
Tst	A	T	15: 78,405,616	M73K	possibly damaging	Het
Tuba4a	C	T	1: 75,216,359	V232M	probably benign	Het
Unk	T	A	11: 116,053,082	V343D	probably benign	Het
Usf3	T	C	16: 44,219,063	V1302A	probably benign	Het
Usp32	A	T	11: 84,994,408	L1271H	probably damaging	Het
Vwa5b2	C	T	16: 20,594,611	P192L	probably benign	Het
Zfp995	A	G	17: 21,882,003	Y38H	probably damaging	Het

Other mutations in Klf17

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Klf17	APN	4	117761038	missense	probably benign	0.06
IGL01399:Klf17	APN	4	117759159	missense	probably damaging	1.00
R0047:Klf17	UTSW	4	117761032	missense	probably benign	0.00
R0051:Klf17	UTSW	4	117760392	missense	probably damaging	1.00
R0051:Klf17	UTSW	4	117760392	missense	probably damaging	1.00
R1513:Klf17	UTSW	4	117760935	missense	probably damaging	0.96
R3103:Klf17	UTSW	4	117760608	missense	possibly damaging	0.72
R4112:Klf17	UTSW	4	117760701	missense	possibly damaging	0.85
R4180:Klf17	UTSW	4	117759186	missense	probably benign	0.14
R4669:Klf17	UTSW	4	117760371	missense	probably damaging	1.00
R4715:Klf17	UTSW	4	117760536	missense	probably benign	0.44
R5063:Klf17	UTSW	4	117760659	missense	possibly damaging	0.85
R7578:Klf17	UTSW	4	117760719	missense	possibly damaging	0.85
R9041:Klf17	UTSW	4	117760359	missense	probably damaging	1.00
Z1176:Klf17	UTSW	4	117760351	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CTCTTGAAGTACTGGGGCTCTC -3'
(R):5'- TACTCTGGGAGTGACCATTTCC -3'

Sequencing Primer
(F):5'- TCTGCCCCCTCTGGAGC -3'
(R):5'- AATCTAATGCCTCAGGGTGGC -3'

Posted On

2019-11-26