Mutagenetix > Incidental Mutation

Incidental Mutation 'R7768:Tas2r103'

598377

Institutional Source

Beutler Lab

Gene Symbol

Tas2r103

Ensembl Gene

ENSMUSG00000030196

Gene Name

taste receptor, type 2, member 103

Synonyms

mt2r63, T2R3, Tas2r10, EG667992, mGR03, TRB2, Tas2r3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R7768 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

133036163-133037101 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 133036849 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 85 (M85V)

Ref Sequence

ENSEMBL: ENSMUSP00000032317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032317]

AlphaFold

Q9JKA3

Predicted Effect

probably benign
Transcript: ENSMUST00000032317
AA Change: M85V

PolyPhen 2 Score 0.062 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000032317
Gene: ENSMUSG00000030196
AA Change: M85V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	5	301	1.5e-79	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700019N19Rik	A	T	19: 58,792,742	Y25N	probably damaging	Het
Actn2	A	G	13: 12,282,594	V479A	possibly damaging	Het
Acy1	G	A	9: 106,433,618	T317M	possibly damaging	Het
Adgrg6	A	T	10: 14,431,666	D797E	probably benign	Het
Ank1	C	T	8: 23,097,997	A552V	probably benign	Het
Ankrd13c	A	G	3: 157,988,647	T262A	probably benign	Het
Ap4e1	A	T	2: 127,046,934	N468Y	probably damaging	Het
Apbb1	T	C	7: 105,567,088	I367V	probably benign	Het
Arl6	A	G	16: 59,632,336	I33T	probably damaging	Het
Asgr2	T	C	11: 70,105,416	I228T	probably damaging	Het
Aspn	A	G	13: 49,557,395	H172R	probably damaging	Het
Bclaf1	T	A	10: 20,339,771	F868L	probably benign	Het
Cacna1i	G	A	15: 80,381,188	R1547H	probably damaging	Het
Celsr1	G	T	15: 85,932,409	Q1778K	probably benign	Het
Cep250	T	C	2: 155,986,009	L42S		Het
Clnk	C	A	5: 38,768,158	R100L	probably damaging	Het
Cpvl	A	G	6: 53,896,491	V420A	possibly damaging	Het
Dicer1	C	A	12: 104,706,697	G825V	probably damaging	Het
Dmxl2	T	C	9: 54,380,939	Y2628C	probably damaging	Het
Dnah7b	T	A	1: 46,137,474	H751Q	probably benign	Het
Dnhd1	C	A	7: 105,721,095	R4576S	possibly damaging	Het
Dzip1	A	T	14: 118,879,498	D775E	probably benign	Het
Erich3	T	A	3: 154,748,331	M381K	probably benign	Het
Fam193a	A	G	5: 34,465,791	D1241G	possibly damaging	Het
Gbx2	A	G	1: 89,928,984	L228P	probably benign	Het
Ggt7	A	T	2: 155,506,501	M77K	possibly damaging	Het
Gm12800	A	C	4: 101,911,813	I454L	probably benign	Het
Gpr26	T	A	7: 131,974,348	I247K	probably damaging	Het
Grip1	A	G	10: 120,038,397	T745A	probably damaging	Het
Gusb	A	T	5: 130,000,405	H178Q	probably benign	Het
Hacl1	T	C	14: 31,616,480	Y380C	probably damaging	Het
Hdac9	T	C	12: 34,390,240	H380R	possibly damaging	Het
Icam4	T	A	9: 21,029,994	L143Q	probably damaging	Het
Ikbkb	T	A	8: 22,695,236	I43F	probably damaging	Het
Ints11	G	T	4: 155,886,939	K303N	probably damaging	Het
Itpa	A	T	2: 130,667,916	N16I	probably damaging	Het
Kank1	GCGAACG	GCG	19: 25,411,205		probably null	Het
Kat6a	T	A	8: 22,903,212	N235K	probably damaging	Het
Kmt2a	C	T	9: 44,820,603	V2806I	unknown	Het
Loxhd1	T	A	18: 77,384,941	F1051L	probably damaging	Het
Map2	A	G	1: 66,414,483	E844G	possibly damaging	Het
Mmp7	T	A	9: 7,697,748	Y261*	probably null	Het
Muc4	A	C	16: 32,756,194	M2023L	unknown	Het
Mybpc1	C	A	10: 88,542,372	G688V	probably damaging	Het
Myo3a	A	G	2: 22,241,143	T34A	probably damaging	Het
Nrxn2	A	G	19: 6,481,379	T683A	possibly damaging	Het
Nup160	A	G	2: 90,700,116	I444V	probably damaging	Het
Olfr1132	T	C	2: 87,635,313	M145V	probably benign	Het
Olfr1291-ps1	A	C	2: 111,499,853	K200N	possibly damaging	Het
Olfr329-ps	A	T	11: 58,542,640	S292T	probably damaging	Het
Olfr329-ps	A	T	11: 58,542,867	M216K	possibly damaging	Het
Pcdha1	A	G	18: 36,932,167	E628G	probably damaging	Het
Pcdha12	T	C	18: 37,022,351	S708P	probably damaging	Het
Pira2	A	T	7: 3,841,697	F445Y	probably benign	Het
Pkd1l2	A	G	8: 117,054,860		probably null	Het
Pla2g6	T	C	15: 79,297,314	D577G	probably damaging	Het
Pla2r1	G	T	2: 60,448,946	D763E	probably benign	Het
Psg18	A	T	7: 18,346,028	I416N	probably damaging	Het
Rapgef6	G	T	11: 54,626,588	V369F	probably damaging	Het
Rasal3	T	C	17: 32,396,793	E357G	probably damaging	Het
Rhbdl3	G	A	11: 80,330,621	R195Q	probably benign	Het
Rnf216	A	T	5: 143,098,444	M1K	probably null	Het
Rspry1	C	A	8: 94,629,841	D165E	probably damaging	Het
Rwdd2b	G	A	16: 87,436,745	L156F	probably benign	Het
Scn11a	T	A	9: 119,815,272	I141F	probably benign	Het
Sirt2	A	T	7: 28,782,859	T198S	probably benign	Het
Slc2a1	A	G	4: 119,132,447	N94D	probably damaging	Het
Slc33a1	A	G	3: 63,947,618	F407S	possibly damaging	Het
Slc38a4	A	C	15: 97,008,664	L356R	probably damaging	Het
Slc43a1	T	A	2: 84,856,871	L372Q	probably damaging	Het
Slc6a6	T	A	6: 91,739,965	I274N	probably damaging	Het
Smchd1	C	T	17: 71,411,911	G821D	probably damaging	Het
Spata33	T	C	8: 123,214,407	F65S	unknown	Het
Stx19	A	G	16: 62,822,204	T128A	probably benign	Het
Tas2r117	C	T	6: 132,803,522	L208F	probably damaging	Het
Tcirg1	A	T	19: 3,902,900	I233N	possibly damaging	Het
Uba6	C	A	5: 86,152,920	W192L	probably benign	Het
Ube3a	T	C	7: 59,288,777	I761T	probably damaging	Het
Unc80	T	C	1: 66,510,595	S671P	possibly damaging	Het
Usp8	A	G	2: 126,751,123	Q766R	probably damaging	Het
Vmn2r103	A	G	17: 19,812,052	N696S	probably damaging	Het
Vmn2r14	G	A	5: 109,220,220	T302I	probably benign	Het
Vmn2r50	G	A	7: 10,037,371	A801V	probably damaging	Het
Vmn2r85	T	G	10: 130,418,693	Q707H	probably damaging	Het
Wsb2	G	A	5: 117,363,722	V51M	probably benign	Het
Xdh	T	C	17: 73,939,836	T78A	probably benign	Het
Zfp442	T	C	2: 150,408,321	K554E	possibly damaging	Het

Other mutations in Tas2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Tas2r103	APN	6	133037056	missense	possibly damaging	0.86
IGL02332:Tas2r103	APN	6	133036512	missense	probably benign	0.21
IGL03122:Tas2r103	APN	6	133036909	missense	probably damaging	1.00
IGL03167:Tas2r103	APN	6	133036660	missense	probably damaging	0.98
R0380:Tas2r103	UTSW	6	133036203	missense	probably damaging	1.00
R0671:Tas2r103	UTSW	6	133036350	missense	probably benign	0.01
R1700:Tas2r103	UTSW	6	133036811	missense	probably damaging	1.00
R2098:Tas2r103	UTSW	6	133036597	missense	probably benign
R3977:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3979:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3980:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R4924:Tas2r103	UTSW	6	133036198	missense	probably benign	0.02
R5779:Tas2r103	UTSW	6	133036945	missense	probably benign	0.02
R5839:Tas2r103	UTSW	6	133036816	missense	probably benign	0.06
R6265:Tas2r103	UTSW	6	133036531	missense	probably damaging	1.00
R8851:Tas2r103	UTSW	6	133036933	missense
R9435:Tas2r103	UTSW	6	133036723	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTTGTACTACAGTTCTGGAGTGTG -3'
(R):5'- GGAAATGTATTCATAGCTCTCGTGAAC -3'

Sequencing Primer
(F):5'- ACTACAGTTCTGGAGTGTGTTTAC -3'
(R):5'- CTCTCGTGAACATCATAGACTGGG -3'

Posted On

2019-11-26