Incidental Mutation 'R7770:Gpr83'
ID598535
Institutional Source Beutler Lab
Gene Symbol Gpr83
Ensembl Gene ENSMUSG00000031932
Gene NameG protein-coupled receptor 83
SynonymsRP82, GIR, Gir, glucocorticoid-induced receptor, RP39, Gpr72, RP105
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.131) question?
Stock #R7770 (G1)
Quality Score225.009
Status Validated
Chromosome9
Chromosomal Location14860210-14870789 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to A at 14866874 bp
ZygosityHeterozygous
Amino Acid Change Arginine to Glutamine at position 180 (R180Q)
Ref Sequence ENSEMBL: ENSMUSP00000034408 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034408] [ENSMUST00000115624]
Predicted Effect probably damaging
Transcript: ENSMUST00000034408
AA Change: R180Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000034408
Gene: ENSMUSG00000031932
AA Change: R180Q

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
Pfam:7tm_4 78 271 2.4e-8 PFAM
Pfam:7TM_GPCR_Srsx 82 360 6.6e-16 PFAM
Pfam:7tm_1 88 345 9e-52 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000115624
AA Change: R138Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000111287
Gene: ENSMUSG00000031932
AA Change: R138Q

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
Pfam:7tm_1 88 133 1.1e-10 PFAM
Pfam:7TM_GPCR_Srsx 128 318 1.8e-6 PFAM
Pfam:7tm_1 129 303 1.4e-28 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.4%
Validation Efficiency 98% (55/56)
MGI Phenotype PHENOTYPE: Mice homozygous for a null allele exhibit normal regulatory T cell development. Mice homozygous for a different targeted allele exhibit attenuated stress-evoked anxiety, increased sucrose preference and delayed spatial learning. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017B05Rik A T 9: 57,258,611 V160E probably damaging Het
Abhd8 C T 8: 71,458,250 G305S probably benign Het
Adamts20 A G 15: 94,333,698 V870A probably benign Het
Agl A G 3: 116,758,237 probably null Het
Cav3 A T 6: 112,472,186 D55V probably damaging Het
Ccser2 T A 14: 36,926,874 D556V probably damaging Het
Cdc20b T C 13: 113,078,659 Y254H probably benign Het
Commd8 A G 5: 72,159,880 L182S probably damaging Het
Cyp1b1 G A 17: 79,713,299 A338V probably damaging Het
Def8 C A 8: 123,460,059 D459E unknown Het
Dennd5b T C 6: 149,041,716 Y554C probably damaging Het
Dnah7c A T 1: 46,626,300 probably null Het
Dnajc5b T A 3: 19,579,017 C135S probably benign Het
Dnpep G A 1: 75,317,246 probably benign Het
Drc1 C T 5: 30,350,512 Q293* probably null Het
Glmp A G 3: 88,325,770 S72G probably benign Het
Gm5431 A T 11: 48,888,458 S546T probably benign Het
Gnptab T A 10: 88,411,920 C70S probably benign Het
Gucy1a1 A T 3: 82,108,805 L292Q possibly damaging Het
Gzmd T C 14: 56,131,263 D58G probably damaging Het
Hoxa10 A G 6: 52,234,265 S224P possibly damaging Het
Hoxa13 G A 6: 52,260,267 probably benign Het
Igsf6 A G 7: 121,068,325 V156A probably benign Het
Il6st A T 13: 112,502,804 I649F probably damaging Het
Kcnh3 G A 15: 99,233,266 V507M probably damaging Het
Lgr5 A G 10: 115,471,994 I253T probably damaging Het
Met T A 6: 17,491,407 V56D possibly damaging Het
Mettl22 A T 16: 8,485,900 I277F possibly damaging Het
Nadsyn1 G A 7: 143,806,003 R411W probably damaging Het
Nrros T G 16: 32,143,528 E557A probably benign Het
Olfr1057 C T 2: 86,375,260 V51M possibly damaging Het
Olfr1134 T C 2: 87,656,469 I151V not run Het
Olfr1338 A T 4: 118,754,057 H162Q probably benign Het
Pih1d2 G A 9: 50,621,801 R243Q not run Het
Polr1b C A 2: 129,125,544 F952L probably damaging Het
Ppp1r3a A G 6: 14,754,978 V90A probably benign Het
Prmt9 T A 8: 77,559,185 probably null Het
Rapgef4 T A 2: 72,198,395 N385K possibly damaging Het
Rbm28 G A 6: 29,164,628 probably benign Het
Rhobtb3 A T 13: 75,917,815 S150T probably damaging Het
Serpinb6b T C 13: 32,977,529 V195A probably benign Het
Sesn1 T A 10: 41,894,058 I99N probably damaging Het
Setd1b G A 5: 123,158,752 probably benign Het
Slc22a19 T A 19: 7,703,995 probably null Het
Slc9a4 T C 1: 40,600,963 I305T probably damaging Het
Smg6 T A 11: 74,993,861 N3K unknown Het
Spta1 C A 1: 174,195,981 Y651* probably null Het
Tatdn3 G A 1: 191,058,856 P74S probably benign Het
Tet2 C T 3: 133,480,295 R1127Q possibly damaging Het
Tgfbi T A 13: 56,632,844 probably null Het
Tmprss9 A G 10: 80,898,069 probably null Het
Tox4 T C 14: 52,279,842 Y10H probably damaging Het
Trappc10 A T 10: 78,210,845 S407T probably damaging Het
Vmn2r59 A G 7: 42,058,912 C24R probably damaging Het
Wdr24 C A 17: 25,827,096 A465D probably benign Het
Wfdc8 A G 2: 164,597,674 S263P unknown Het
Zc3h15 T A 2: 83,658,132 I138N possibly damaging Het
Other mutations in Gpr83
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00579:Gpr83 APN 9 14860601 missense probably benign 0.00
R0243:Gpr83 UTSW 9 14864842 missense possibly damaging 0.96
R0349:Gpr83 UTSW 9 14868267 missense probably damaging 1.00
R0731:Gpr83 UTSW 9 14868644 missense probably benign 0.00
R1519:Gpr83 UTSW 9 14868197 missense probably null 1.00
R1678:Gpr83 UTSW 9 14866849 missense probably damaging 1.00
R1826:Gpr83 UTSW 9 14868333 missense possibly damaging 0.53
R1827:Gpr83 UTSW 9 14868333 missense possibly damaging 0.53
R1828:Gpr83 UTSW 9 14868333 missense possibly damaging 0.53
R2938:Gpr83 UTSW 9 14864871 missense probably benign 0.23
R3760:Gpr83 UTSW 9 14860738 missense probably benign 0.03
R4038:Gpr83 UTSW 9 14860777 missense possibly damaging 0.77
R4438:Gpr83 UTSW 9 14864838 missense probably damaging 0.99
R4657:Gpr83 UTSW 9 14866983 critical splice donor site probably null
R4731:Gpr83 UTSW 9 14866174 intron probably benign
R5150:Gpr83 UTSW 9 14860805 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCAGGAGTCACAGAGTCTACATG -3'
(R):5'- TTCTGGAGGTCAGCATGGTAC -3'

Sequencing Primer
(F):5'- TCTTCCCACCCCTGGAGG -3'
(R):5'- TGGTACACTCTCCCTTGAGC -3'
Posted On2019-11-26