Incidental Mutation 'R7773:Gm853'
ID598696
Institutional Source Beutler Lab
Gene Symbol Gm853
Ensembl Gene ENSMUSG00000023120
Gene Namepredicted gene 853
SynonymsLOC332942
Accession Numbers

Genbank: NM_001034872.2; Ensembl: ENSMUST00000023884

Is this an essential gene? Probably non essential (E-score: 0.106) question?
Stock #R7773 (G1)
Quality Score225.009
Status Validated
Chromosome4
Chromosomal Location130209109-130222401 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 130220376 bp
ZygosityHeterozygous
Amino Acid Change Asparagine to Aspartic acid at position 83 (N83D)
Ref Sequence ENSEMBL: ENSMUSP00000023884 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000023884]
Predicted Effect probably damaging
Transcript: ENSMUST00000023884
AA Change: N83D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000023884
Gene: ENSMUSG00000023120
AA Change: N83D

DomainStartEndE-ValueType
Pfam:Orn_Arg_deC_N 56 294 3.6e-79 PFAM
Pfam:Orn_DAP_Arg_deC 263 410 4.3e-23 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.3%
Validation Efficiency 98% (55/56)
Allele List at MGI

All alleles(2) : Targeted, other(2)

Other mutations in this stock
Total: 59 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700006A11Rik A G 3: 124,412,531 I336T probably benign Het
4930519P11Rik T C 2: 154,613,187 Y84C unknown Het
4930568D16Rik C T 2: 35,354,594 G249S probably damaging Het
4933425L06Rik A G 13: 105,082,285 I42M probably damaging Het
Adprhl1 C T 8: 13,248,682 V83I probably damaging Het
Agbl1 T A 7: 76,698,837 V894E unknown Het
Amigo3 T C 9: 108,054,668 L430P probably benign Het
Aoc1 A G 6: 48,906,212 I341V probably benign Het
Bod1l A G 5: 41,832,712 S223P probably benign Het
Cacnb3 T C 15: 98,639,938 probably null Het
Ccdc63 G T 5: 122,109,272 N503K probably damaging Het
Ccdc83 T A 7: 90,229,912 I225F probably damaging Het
Cd200r1 A G 16: 44,789,687 T90A possibly damaging Het
Cd8a A G 6: 71,373,815 N88S probably benign Het
Cep170 A G 1: 176,740,076 V152A Het
Chid1 T C 7: 141,529,605 M123V probably benign Het
Cnnm4 T C 1: 36,499,522 V595A probably benign Het
Coro7 A G 16: 4,632,006 L630P probably damaging Het
Cpvl A G 6: 53,931,905 probably null Het
Edem3 A T 1: 151,811,596 K762* probably null Het
Elp6 T A 9: 110,312,559 probably null Het
Emilin3 A T 2: 160,910,798 Y77* probably null Het
Fam234b T A 6: 135,243,914 I641N probably benign Het
Farsa T C 8: 84,864,152 probably null Het
Foxf2 T C 13: 31,627,199 S374P probably benign Het
Gm11596 A T 11: 99,792,841 I151N unknown Het
Gm13178 A T 4: 144,703,477 L314Q probably damaging Het
Gm9817 C T 13: 45,078,951 Q77* probably null Het
Gon4l A G 3: 88,895,795 K1238E probably benign Het
H13 A G 2: 152,695,511 Y292C probably damaging Het
Hip1r A G 5: 124,001,441 N928S probably benign Het
Iqcf4 T C 9: 106,568,613 N112D probably benign Het
Jak3 A C 8: 71,679,042 T125P probably benign Het
Krt7 A C 15: 101,414,032 K124Q possibly damaging Het
Lrrc66 G A 5: 73,607,321 S793F probably damaging Het
Mycbp2 C T 14: 103,248,404 V1074I probably damaging Het
Nckap5 T C 1: 126,026,844 D657G probably benign Het
Olfr1053 T C 2: 86,314,690 I199V probably benign Het
Olfr1467 T A 19: 13,365,234 V202E probably benign Het
Osbpl7 A G 11: 97,050,722 S24G probably benign Het
Pcm1 G T 8: 41,309,573 E1385* probably null Het
Poc5 A G 13: 96,410,635 T469A probably damaging Het
Ppp3ca A G 3: 136,890,461 T296A probably benign Het
Prl6a1 T C 13: 27,318,142 I164T probably damaging Het
Psmd6 GCAGAGCGGGCAGGGCATCTCACTGACCCTGTCACCTACCCAGAGCGGGCAGGGCATCTCACTGACCCTGTCACCTACCCAGAGCGGGCAGGGCATCTCACTGACC GCAGAGCGGGCAGGGCATCTCACTGACCCTGTCACCTACCCAGAGCGGGCAGGGCATCTCACTGACC 14: 14,119,882 probably null Het
Rnf149 A C 1: 39,565,218 M188R possibly damaging Het
Rpl10l T C 12: 66,284,267 I31V probably benign Het
Serinc5 T C 13: 92,661,084 S32P probably damaging Het
Sirt1 T A 10: 63,326,783 K137M possibly damaging Het
Smc4 A G 3: 69,016,163 Y251C probably damaging Het
Sod3 A C 5: 52,368,301 E114A possibly damaging Het
Tgfbr3 A G 5: 107,140,502 V431A probably benign Het
Tlr4 T C 4: 66,839,599 S210P probably damaging Het
Trub2 G T 2: 29,786,508 T70N probably benign Het
Tsga10 A T 1: 37,835,242 C159S unknown Het
Vmn1r15 A T 6: 57,258,659 I171F probably benign Het
Vmn2r50 T A 7: 10,037,635 H713L possibly damaging Het
Zcchc14 T C 8: 121,651,775 S43G unknown Het
Zfp638 T C 6: 83,979,214 I1601T probably damaging Het
Other mutations in Gm853
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01111:Gm853 APN 4 130221725 missense probably benign 0.01
IGL01485:Gm853 APN 4 130215425 missense probably benign 0.02
1mM(1):Gm853 UTSW 4 130216581 missense probably benign 0.04
PIT4382001:Gm853 UTSW 4 130219161 missense possibly damaging 0.94
R0762:Gm853 UTSW 4 130221624 missense probably damaging 0.98
R0846:Gm853 UTSW 4 130221624 missense probably benign 0.10
R1070:Gm853 UTSW 4 130219156 missense probably benign 0.14
R1918:Gm853 UTSW 4 130211393 missense probably benign
R2117:Gm853 UTSW 4 130215363 missense possibly damaging 0.53
R2566:Gm853 UTSW 4 130209888 missense probably benign 0.14
R4110:Gm853 UTSW 4 130219174 missense probably damaging 0.99
R5033:Gm853 UTSW 4 130221615 critical splice donor site probably null
R5658:Gm853 UTSW 4 130220441 missense probably benign 0.00
R5751:Gm853 UTSW 4 130220441 missense probably benign 0.05
R6283:Gm853 UTSW 4 130221741 missense probably benign 0.00
R6993:Gm853 UTSW 4 130218313 missense probably damaging 1.00
R7224:Gm853 UTSW 4 130219199 missense probably damaging 1.00
R8254:Gm853 UTSW 4 130220343 missense probably benign 0.06
R8329:Gm853 UTSW 4 130215363 missense possibly damaging 0.86
Z1176:Gm853 UTSW 4 130221704 missense probably benign
Predicted Primers PCR Primer
(F):5'- AGGCCGACTGTAGCAATCTG -3'
(R):5'- TCGTCATAAAGTGGAGAGGCC -3'

Sequencing Primer
(F):5'- GAGTGAATTCTAACCTAGCCTGGAC -3'
(R):5'- AGAGGCCCTGAGGACCTAAC -3'
Posted On2019-11-26