Incidental Mutation 'R7774:H4c16'
ID 598762
Institutional Source Beutler Lab
Gene Symbol H4c16
Ensembl Gene ENSMUSG00000096010
Gene Name H4 histone 16
Synonyms H4-16, H4f16, B130044J01Rik, Hist4h4
MMRRC Submission 045830-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.947) question?
Stock # R7774 (G1)
Quality Score 225.009
Status Validated
Chromosome 6
Chromosomal Location 136780991-136781429 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 136781281 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Proline to Threonine at position 33 (P33T)
Ref Sequence ENSEMBL: ENSMUSP00000136357 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000074556] [ENSMUST00000179285] [ENSMUST00000203982]
AlphaFold no structure available at present
Predicted Effect probably benign
Transcript: ENSMUST00000074556
SMART Domains Protein: ENSMUSP00000074142
Gene: ENSMUSG00000060032

DomainStartEndE-ValueType
H2A 3 123 2.32e-81 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000179285
AA Change: P33T

PolyPhen 2 Score 0.926 (Sensitivity: 0.81; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000136357
Gene: ENSMUSG00000096010
AA Change: P33T

DomainStartEndE-ValueType
H4 16 90 2.59e-29 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000203982
SMART Domains Protein: ENSMUSP00000144974
Gene: ENSMUSG00000060032

DomainStartEndE-ValueType
H2A 3 123 9.8e-84 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (64/64)
MGI Phenotype FUNCTION: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. [provided by RefSeq, Aug 2015]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adcy6 G T 15: 98,494,414 (GRCm39) T809N probably benign Het
Adgb C A 10: 10,215,404 (GRCm39) E1561* probably null Het
Adgra1 A T 7: 139,427,628 (GRCm39) H65L possibly damaging Het
Adprhl1 C T 8: 13,298,682 (GRCm39) V83I probably damaging Het
Atrnl1 G T 19: 57,688,103 (GRCm39) G856V probably damaging Het
Cc2d2b A G 19: 40,754,161 (GRCm39) K177E unknown Het
Ccdc122 T C 14: 77,305,379 (GRCm39) V11A probably benign Het
Clasp2 T A 9: 113,677,804 (GRCm39) probably null Het
Col6a1 T C 10: 76,545,710 (GRCm39) T921A unknown Het
Cul3 A C 1: 80,247,011 (GRCm39) D697E probably benign Het
Defa34 G A 8: 22,155,978 (GRCm39) E56K probably benign Het
Dhrs7c A G 11: 67,700,641 (GRCm39) R63G probably damaging Het
Dnaaf11 T G 15: 66,321,401 (GRCm39) I247L probably benign Het
Dnah3 T A 7: 119,550,975 (GRCm39) K136* probably null Het
Exoc7 T C 11: 116,186,142 (GRCm39) D353G possibly damaging Het
Fbxw21 T C 9: 108,972,908 (GRCm39) Y342C probably benign Het
Fitm2 T A 2: 163,311,986 (GRCm39) I76F probably damaging Het
Fryl A T 5: 73,240,727 (GRCm39) I1291N probably benign Het
Fzd2 A T 11: 102,496,314 (GRCm39) I253F possibly damaging Het
Gm5592 A T 7: 40,939,283 (GRCm39) Y855F probably damaging Het
H1f6 A G 13: 23,880,183 (GRCm39) K112R possibly damaging Het
Helz2 T C 2: 180,875,784 (GRCm39) Y1570C probably benign Het
Ints11 A G 4: 155,970,140 (GRCm39) T228A probably benign Het
Ipo13 G T 4: 117,771,494 (GRCm39) N25K probably benign Het
Itga9 T A 9: 118,700,968 (GRCm39) I917N probably damaging Het
Krt39 T C 11: 99,405,437 (GRCm39) probably null Het
Krtap13-1 C T 16: 88,526,061 (GRCm39) T95I possibly damaging Het
Ldlrad4 G T 18: 68,368,863 (GRCm39) E107* probably null Het
Ly6a A T 15: 74,869,416 (GRCm39) I13N probably damaging Het
Mfsd4b4 T C 10: 39,768,407 (GRCm39) T275A probably benign Het
Mgat3 C T 15: 80,095,743 (GRCm39) T190M probably damaging Het
Muc5b G A 7: 141,396,116 (GRCm39) R124H unknown Het
Mucl1 G T 15: 103,783,950 (GRCm39) N85K possibly damaging Het
Nifk G A 1: 118,255,391 (GRCm39) E96K possibly damaging Het
Opn3 C T 1: 175,490,471 (GRCm39) V397M probably damaging Het
Or52ad1 T C 7: 102,995,737 (GRCm39) R133G possibly damaging Het
Or5ae1 T A 7: 84,565,739 (GRCm39) F251I probably damaging Het
Or8c17 T C 9: 38,180,655 (GRCm39) V274A probably damaging Het
Pcdha2 A G 18: 37,074,579 (GRCm39) M737V probably benign Het
Pdk4 T A 6: 5,492,757 (GRCm39) D98V possibly damaging Het
Pkhd1l1 A G 15: 44,404,303 (GRCm39) T2311A probably benign Het
Pla2r1 A T 2: 60,360,802 (GRCm39) C195* probably null Het
Polr1b C A 2: 128,967,464 (GRCm39) F952L probably damaging Het
Pramel23 C T 4: 143,423,676 (GRCm39) S371N possibly damaging Het
Ptprq T C 10: 107,479,530 (GRCm39) T1166A probably damaging Het
Ran G A 5: 129,099,874 (GRCm39) D215N probably benign Het
Rb1cc1 T C 1: 6,318,309 (GRCm39) F604L possibly damaging Het
Rgl1 T A 1: 152,430,101 (GRCm39) E227D probably benign Het
Sec24b C A 3: 129,777,846 (GRCm39) R1204L possibly damaging Het
Shroom3 T C 5: 93,098,348 (GRCm39) L1276P probably damaging Het
Smarcad1 T A 6: 65,084,814 (GRCm39) M820K probably damaging Het
Sptbn1 A T 11: 30,092,142 (GRCm39) M541K probably damaging Het
Tcp11l2 T C 10: 84,440,847 (GRCm39) V351A possibly damaging Het
Tecpr2 A G 12: 110,899,606 (GRCm39) D658G probably benign Het
Tlr6 T G 5: 65,110,728 (GRCm39) E726D probably damaging Het
Tmem218 T A 9: 37,133,864 (GRCm39) H101Q probably benign Het
Tnfrsf4 C T 4: 156,098,795 (GRCm39) Q82* probably null Het
Trpm7 A G 2: 126,655,158 (GRCm39) V1260A probably benign Het
Trpm8 A T 1: 88,258,563 (GRCm39) E282V probably damaging Het
Tuba8 T C 6: 121,200,348 (GRCm39) V344A probably damaging Het
Tvp23a A G 16: 10,245,245 (GRCm39) probably null Het
Zfp174 G A 16: 3,667,215 (GRCm39) V135M probably damaging Het
Zfp418 G A 7: 7,185,776 (GRCm39) V580I possibly damaging Het
Zfp451 T A 1: 33,844,474 (GRCm39) E44D probably benign Het
Zfp521 T C 18: 13,978,838 (GRCm39) D525G probably benign Het
Other mutations in H4c16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02567:H4c16 APN 6 136,781,272 (GRCm39) missense probably damaging 0.97
IGL02625:H4c16 APN 6 136,781,335 (GRCm39) unclassified probably benign
R2044:H4c16 UTSW 6 136,781,101 (GRCm39) missense possibly damaging 0.73
R2276:H4c16 UTSW 6 136,781,299 (GRCm39) missense probably damaging 1.00
R5077:H4c16 UTSW 6 136,781,113 (GRCm39) missense probably benign 0.17
R5868:H4c16 UTSW 6 136,781,292 (GRCm39) missense probably damaging 1.00
R8076:H4c16 UTSW 6 136,781,124 (GRCm39) missense probably benign 0.15
Predicted Primers PCR Primer
(F):5'- AAAAGGGCCTTTGTGGATGG -3'
(R):5'- CGCTCCCAGGAAATATAAGCTC -3'

Sequencing Primer
(F):5'- ACAGGAGGGCTCGCTTAG -3'
(R):5'- GGCGGATTACTGTAGTTTCT -3'
Posted On 2019-11-26