Mutagenetix > Incidental Mutation

Incidental Mutation 'R7780:Or6k6'

599136

Institutional Source

Beutler Lab

Gene Symbol

Or6k6

Ensembl Gene

ENSMUSG00000046486

Gene Name

olfactory receptor family 6 subfamily K member 6

Synonyms

Olfr425, GA_x6K02T02K39-452-3, Olfr244, Olfr231, GA_x6K02T2P20D-21053942-21054499, GA_x6K02T2LER7-6-623, MOR105-4

MMRRC Submission

045836-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.169) question?

Stock #

R7780 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

173944657-173945580 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 173945115 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 156 (I156V)

Ref Sequence

ENSEMBL: ENSMUSP00000151011 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063030] [ENSMUST00000214446]

AlphaFold

E9Q0M4

Predicted Effect

probably benign
Transcript: ENSMUST00000063030
AA Change: I156V

PolyPhen 2 Score 0.016 (Sensitivity: 0.95; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000059596
Gene: ENSMUSG00000046486
AA Change: I156V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	24	300	2.6e-63	PFAM
Pfam:7tm_1	34	282	2.7e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214446
AA Change: I156V

PolyPhen 2 Score 0.016 (Sensitivity: 0.95; Specificity: 0.79)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (77/77)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam5	C	T	8: 25,294,432 (GRCm39)	R389K	possibly damaging	Het
Ahnak2	C	T	12: 112,746,716 (GRCm39)	V1205M		Het
Ccr3	A	G	9: 123,828,989 (GRCm39)	Y108C	probably benign	Het
Cers5	T	G	15: 99,637,589 (GRCm39)	H220P	probably damaging	Het
Cfap61	A	T	2: 145,995,692 (GRCm39)	N1086Y	possibly damaging	Het
Cip2a	T	A	16: 48,822,023 (GRCm39)	L217*	probably null	Het
Clstn3	T	C	6: 124,439,161 (GRCm39)	E32G	probably damaging	Het
Cox20	T	C	1: 178,149,566 (GRCm39)	C72R	probably benign	Het
Csf1	A	T	3: 107,657,709 (GRCm39)	N154K	probably damaging	Het
Cyp2j7	T	A	4: 96,118,256 (GRCm39)	K112N	probably benign	Het
Drosha	A	G	15: 12,848,172 (GRCm39)	D488G	probably benign	Het
Edem1	A	G	6: 108,818,589 (GRCm39)	K199R	probably benign	Het
Esyt2	T	C	12: 116,305,718 (GRCm39)	I316T	probably benign	Het
Evpl	C	T	11: 116,125,000 (GRCm39)	R154H	not run	Het
Fbn1	A	G	2: 125,143,678 (GRCm39)	L2817P	probably benign	Het
Fbxo42	T	A	4: 140,921,131 (GRCm39)		probably null	Het
Fbxo43	T	C	15: 36,162,358 (GRCm39)	D283G	probably damaging	Het
Fdxr	A	T	11: 115,167,656 (GRCm39)	S20T	probably benign	Het
Fkbp10	T	C	11: 100,312,048 (GRCm39)	Y203H	probably damaging	Het
Galntl6	C	T	8: 58,880,733 (GRCm39)		probably null	Het
Gask1b	G	A	3: 79,848,711 (GRCm39)	G486S	probably damaging	Het
Gm26657	C	A	4: 56,741,056 (GRCm39)	Y80*	probably null	Het
Gm973	T	A	1: 59,597,289 (GRCm39)	W440R	probably damaging	Het
Helz	T	A	11: 107,528,689 (GRCm39)	F903Y	probably damaging	Het
Iqcf4	T	A	9: 106,445,860 (GRCm39)	I96F	possibly damaging	Het
Kcnq5	T	A	1: 22,031,555 (GRCm39)	Q84L	probably benign	Het
Krit1	G	A	5: 3,862,772 (GRCm39)	G254S	probably damaging	Het
Krtap6-2	A	T	16: 89,216,510 (GRCm39)	Y152*	probably null	Het
Loricrin	A	T	3: 91,988,460 (GRCm39)	Y275*	probably null	Het
Map4	T	C	9: 109,863,720 (GRCm39)	I315T	probably benign	Het
Mcpt1	T	A	14: 56,256,609 (GRCm39)		probably null	Het
Mdh1b	C	A	1: 63,759,133 (GRCm39)	S153I	possibly damaging	Het
Mfsd12	C	A	10: 81,193,718 (GRCm39)	A96E	probably benign	Het
Mki67	T	C	7: 135,315,697 (GRCm39)	E55G	probably benign	Het
Mterf4	T	C	1: 93,232,689 (GRCm39)	E54G	probably benign	Het
Mucl3	A	G	17: 35,947,874 (GRCm39)	S452P	possibly damaging	Het
Nuggc	T	A	14: 65,882,490 (GRCm39)	I745N	probably damaging	Het
Or7g18	A	G	9: 18,786,910 (GRCm39)	T93A	possibly damaging	Het
Parg	A	T	14: 31,930,758 (GRCm39)	N126I	possibly damaging	Het
Pcdh9	T	C	14: 94,123,987 (GRCm39)	I728V	possibly damaging	Het
Pcdha1	C	G	18: 37,065,511 (GRCm39)	P725R	probably benign	Het
Pcdha11	T	A	18: 37,145,849 (GRCm39)	L647M	probably damaging	Het
Phb2	T	C	6: 124,692,995 (GRCm39)		probably null	Het
Plb1	A	G	5: 32,483,610 (GRCm39)		probably null	Het
Pmch	T	A	10: 87,927,113 (GRCm39)	F39I	probably benign	Het
Psmd7	A	T	8: 108,307,920 (GRCm39)	S188T	possibly damaging	Het
Pycr2	T	A	1: 180,733,913 (GRCm39)	D168E	probably damaging	Het
Rab43	A	G	6: 87,771,692 (GRCm39)	Y96H	probably damaging	Het
Ralgapa2	C	T	2: 146,184,334 (GRCm39)	G1511S	probably benign	Het
Rb1cc1	T	A	1: 6,319,138 (GRCm39)	C852*	probably null	Het
Rexo2	T	C	9: 48,380,145 (GRCm39)	N229S	probably damaging	Het
Sema4f	A	G	6: 82,890,941 (GRCm39)	V622A	possibly damaging	Het
Serpina10	T	C	12: 103,594,806 (GRCm39)	S138G	probably benign	Het
Sptbn4	T	A	7: 27,061,059 (GRCm39)	T2415S	possibly damaging	Het
Tbc1d22b	A	G	17: 29,792,040 (GRCm39)	N257D	probably benign	Het
Tbl3	A	G	17: 24,921,205 (GRCm39)	F529L	probably damaging	Het
Them6	A	T	15: 74,593,427 (GRCm39)	N95I	probably benign	Het
Thrb	T	C	14: 18,008,608 (GRCm38)	S156P	possibly damaging	Het
Tmem186	G	A	16: 8,453,731 (GRCm39)	R177C	probably benign	Het
Ttn	T	A	2: 76,660,937 (GRCm39)		probably null	Het
Unc13a	T	A	8: 72,110,979 (GRCm39)	I411F	probably benign	Het
Uqcc1	T	C	2: 155,752,073 (GRCm39)		probably null	Het
Vmn1r56	T	C	7: 5,199,516 (GRCm39)	T34A	possibly damaging	Het
Vps39	A	T	2: 120,155,680 (GRCm39)	Y542*	probably null	Het
Wdr64	T	C	1: 175,556,542 (GRCm39)	L263P	probably damaging	Het
Wdr90	C	T	17: 26,065,300 (GRCm39)	R1652Q	probably damaging	Het
Wfdc16	A	G	2: 164,477,785 (GRCm39)	M88T	probably benign	Het
Wnk4	A	G	11: 101,160,403 (GRCm39)	D679G	probably damaging	Het
Ykt6	T	C	11: 5,912,751 (GRCm39)	I151T	probably benign	Het
Zbtb46	A	G	2: 181,033,225 (GRCm39)	C479R	probably damaging	Het
Zc3h7a	A	G	16: 10,967,115 (GRCm39)	I559T	probably benign	Het
Zfhx3	A	G	8: 109,678,283 (GRCm39)	N3111S	possibly damaging	Het
Zfp619	T	C	7: 39,184,432 (GRCm39)	V154A	possibly damaging	Het
Zfp629	A	T	7: 127,211,601 (GRCm39)	D69E	probably benign	Het
Zfp882	T	A	8: 72,668,073 (GRCm39)	I300N	possibly damaging	Het
Zfp934	T	C	13: 62,666,358 (GRCm39)	H106R	possibly damaging	Het
Zfta	T	C	19: 7,399,737 (GRCm39)	S495P	probably damaging	Het
Zfyve9	G	A	4: 108,576,298 (GRCm39)	T261I	possibly damaging	Het

Other mutations in Or6k6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01973:Or6k6	APN	1	173,945,099 (GRCm39)	missense	probably damaging	1.00
IGL03126:Or6k6	APN	1	173,945,276 (GRCm39)	missense	probably benign	0.07
R0200:Or6k6	UTSW	1	173,945,078 (GRCm39)	missense	probably benign	0.00
R1292:Or6k6	UTSW	1	173,945,420 (GRCm39)	missense	probably benign	0.16
R1966:Or6k6	UTSW	1	173,944,817 (GRCm39)	nonsense	probably null
R2860:Or6k6	UTSW	1	173,945,298 (GRCm39)	missense	probably damaging	1.00
R2861:Or6k6	UTSW	1	173,945,298 (GRCm39)	missense	probably damaging	1.00
R2862:Or6k6	UTSW	1	173,945,298 (GRCm39)	missense	probably damaging	1.00
R4492:Or6k6	UTSW	1	173,944,770 (GRCm39)	missense	probably benign	0.01
R4594:Or6k6	UTSW	1	173,944,886 (GRCm39)	missense	probably damaging	0.96
R4670:Or6k6	UTSW	1	173,945,427 (GRCm39)	missense	probably benign	0.04
R4703:Or6k6	UTSW	1	173,944,964 (GRCm39)	missense	possibly damaging	0.52
R6385:Or6k6	UTSW	1	173,944,862 (GRCm39)	missense	probably damaging	1.00
R7085:Or6k6	UTSW	1	173,945,226 (GRCm39)	missense	probably damaging	1.00
R7426:Or6k6	UTSW	1	173,944,753 (GRCm39)	missense	probably benign	0.00
R7708:Or6k6	UTSW	1	173,945,300 (GRCm39)	missense	probably damaging	0.99
R9308:Or6k6	UTSW	1	173,945,501 (GRCm39)	missense	probably benign	0.00
Z1088:Or6k6	UTSW	1	173,944,881 (GRCm39)	missense	probably benign	0.38

Predicted Primers

PCR Primer
(F):5'- TGATTCGGATATAGGACAGGGC -3'
(R):5'- GTGAGAAGAAGACCATCTCCCTAG -3'

Sequencing Primer
(F):5'- TTCGGATATAGGACAGGGCAATTAC -3'
(R):5'- TAGGTGGCTGCCTCATGC -3'

Posted On

2019-11-26