Mutagenetix > Incidental Mutations

Incidental Mutation 'R7780:Zfp882'

599167

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.148) question?

Stock #

R7780 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 71914229 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 300 (I300N)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110002
AA Change: I300N

PolyPhen 2 Score 0.892 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: I300N

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (77/77)

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700081O15Rik	T	C	19: 7,422,372	S495P	probably damaging	Het
Adam5	C	T	8: 24,804,416	R389K	possibly damaging	Het
Ahnak2	C	T	12: 112,782,613	V1205M		Het
C330027C09Rik	T	A	16: 49,001,660	L217*	probably null	Het
Ccr3	A	G	9: 124,028,952	Y108C	probably benign	Het
Cers5	T	G	15: 99,739,708	H220P	probably damaging	Het
Cfap61	A	T	2: 146,153,772	N1086Y	possibly damaging	Het
Clstn3	T	C	6: 124,462,202	E32G	probably damaging	Het
Cox20	T	C	1: 178,322,001	C72R	probably benign	Het
Csf1	A	T	3: 107,750,393	N154K	probably damaging	Het
Cyp2j7	T	A	4: 96,230,019	K112N	probably benign	Het
Dpcr1	A	G	17: 35,636,982	S452P	possibly damaging	Het
Drosha	A	G	15: 12,848,086	D488G	probably benign	Het
Edem1	A	G	6: 108,841,628	K199R	probably benign	Het
Esyt2	T	C	12: 116,342,098	I316T	probably benign	Het
Evpl	C	T	11: 116,234,174	R154H	not run	Het
Fam198b	G	A	3: 79,941,404	G486S	probably damaging	Het
Fbn1	A	G	2: 125,301,758	L2817P	probably benign	Het
Fbxo42	T	A	4: 141,193,820		probably null	Het
Fbxo43	T	C	15: 36,162,212	D283G	probably damaging	Het
Fdxr	A	T	11: 115,276,830	S20T	probably benign	Het
Fkbp10	T	C	11: 100,421,222	Y203H	probably damaging	Het
Galntl6	C	T	8: 58,427,699		probably null	Het
Gm26657	C	A	4: 56,741,056	Y80*	probably null	Het
Gm973	T	A	1: 59,558,130	W440R	probably damaging	Het
Helz	T	A	11: 107,637,863	F903Y	probably damaging	Het
Iqcf4	T	A	9: 106,568,661	I96F	possibly damaging	Het
Kcnq5	T	A	1: 21,961,331	Q84L	probably benign	Het
Krit1	G	A	5: 3,812,772	G254S	probably damaging	Het
Krtap6-2	A	T	16: 89,419,622	Y152*	probably null	Het
Lor	A	T	3: 92,081,153	Y275*	probably null	Het
Map4	T	C	9: 110,034,652	I315T	probably benign	Het
Mcpt1	T	A	14: 56,019,152		probably null	Het
Mdh1b	C	A	1: 63,719,974	S153I	possibly damaging	Het
Mfsd12	C	A	10: 81,357,884	A96E	probably benign	Het
Mki67	T	C	7: 135,713,968	E55G	probably benign	Het
Mterf4	T	C	1: 93,304,967	E54G	probably benign	Het
Nuggc	T	A	14: 65,645,041	I745N	probably damaging	Het
Olfr231	T	C	1: 174,117,549	I156V	probably benign	Het
Olfr830	A	G	9: 18,875,614	T93A	possibly damaging	Het
Parg	A	T	14: 32,208,801	N126I	possibly damaging	Het
Pcdh9	T	C	14: 93,886,551	I728V	possibly damaging	Het
Pcdha1	C	G	18: 36,932,458	P725R	probably benign	Het
Pcdha11	T	A	18: 37,012,796	L647M	probably damaging	Het
Phb2	T	C	6: 124,716,032		probably null	Het
Plb1	A	G	5: 32,326,266		probably null	Het
Pmch	T	A	10: 88,091,251	F39I	probably benign	Het
Psmd7	A	T	8: 107,581,288	S188T	possibly damaging	Het
Pycr2	T	A	1: 180,906,348	D168E	probably damaging	Het
Rab43	A	G	6: 87,794,710	Y96H	probably damaging	Het
Ralgapa2	C	T	2: 146,342,414	G1511S	probably benign	Het
Rb1cc1	T	A	1: 6,248,914	C852*	probably null	Het
Rexo2	T	C	9: 48,468,845	N229S	probably damaging	Het
Sema4f	A	G	6: 82,913,960	V622A	possibly damaging	Het
Serpina10	T	C	12: 103,628,547	S138G	probably benign	Het
Sptbn4	T	A	7: 27,361,634	T2415S	possibly damaging	Het
Tbc1d22b	A	G	17: 29,573,066	N257D	probably benign	Het
Tbl3	A	G	17: 24,702,231	F529L	probably damaging	Het
Them6	A	T	15: 74,721,578	N95I	probably benign	Het
Thrb	T	C	14: 18,008,608	S156P	possibly damaging	Het
Tmem186	G	A	16: 8,635,867	R177C	probably benign	Het
Ttn	T	A	2: 76,830,593		probably null	Het
Unc13a	T	A	8: 71,658,335	I411F	probably benign	Het
Uqcc1	T	C	2: 155,910,153		probably null	Het
Vmn1r56	T	C	7: 5,196,517	T34A	possibly damaging	Het
Vps39	A	T	2: 120,325,199	Y542*	probably null	Het
Wdr64	T	C	1: 175,728,976	L263P	probably damaging	Het
Wdr90	C	T	17: 25,846,326	R1652Q	probably damaging	Het
Wfdc16	A	G	2: 164,635,865	M88T	probably benign	Het
Wnk4	A	G	11: 101,269,577	D679G	probably damaging	Het
Ykt6	T	C	11: 5,962,751	I151T	probably benign	Het
Zbtb46	A	G	2: 181,391,432	C479R	probably damaging	Het
Zc3h7a	A	G	16: 11,149,251	I559T	probably benign	Het
Zfhx3	A	G	8: 108,951,651	N3111S	possibly damaging	Het
Zfp619	T	C	7: 39,535,008	V154A	possibly damaging	Het
Zfp629	A	T	7: 127,612,429	D69E	probably benign	Het
Zfp934	T	C	13: 62,518,544	H106R	possibly damaging	Het
Zfyve9	G	A	4: 108,719,101	T261I	possibly damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71913827	missense	probably benign
R0244:Zfp882	UTSW	8	71913523	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	71914615	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71914337	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71914686	nonsense	probably null
R1299:Zfp882	UTSW	8	71913473	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	71913609	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	71914389	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71914654	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71914360	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71913459	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	71913155	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	71914505	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	71914640	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	71914286	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	71914673	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71913249	critical splice donor site	probably null
R7793:Zfp882	UTSW	8	71913141	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	71914118	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTTTGAGGAAGGTTGAAAGTCATG -3'
(R):5'- CCCTGTGAATCTGTTCATGGTATC -3'

Sequencing Primer
(F):5'- GGTTGAAAGTCATGAACAAACTCAC -3'
(R):5'- GTGATGCATGAAGGCTTTCCCAC -3'

Posted On

2019-11-26