Mutagenetix > Incidental Mutation

Incidental Mutation 'R7780:Zfp882'

599167

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

045836-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R7780 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

72662452-72670198 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 72668073 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 300 (I300N)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110002
AA Change: I300N

PolyPhen 2 Score 0.892 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: I300N

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (77/77)

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam5	C	T	8: 25,294,432 (GRCm39)	R389K	possibly damaging	Het
Ahnak2	C	T	12: 112,746,716 (GRCm39)	V1205M		Het
Ccr3	A	G	9: 123,828,989 (GRCm39)	Y108C	probably benign	Het
Cers5	T	G	15: 99,637,589 (GRCm39)	H220P	probably damaging	Het
Cfap61	A	T	2: 145,995,692 (GRCm39)	N1086Y	possibly damaging	Het
Cip2a	T	A	16: 48,822,023 (GRCm39)	L217*	probably null	Het
Clstn3	T	C	6: 124,439,161 (GRCm39)	E32G	probably damaging	Het
Cox20	T	C	1: 178,149,566 (GRCm39)	C72R	probably benign	Het
Csf1	A	T	3: 107,657,709 (GRCm39)	N154K	probably damaging	Het
Cyp2j7	T	A	4: 96,118,256 (GRCm39)	K112N	probably benign	Het
Drosha	A	G	15: 12,848,172 (GRCm39)	D488G	probably benign	Het
Edem1	A	G	6: 108,818,589 (GRCm39)	K199R	probably benign	Het
Esyt2	T	C	12: 116,305,718 (GRCm39)	I316T	probably benign	Het
Evpl	C	T	11: 116,125,000 (GRCm39)	R154H	not run	Het
Fbn1	A	G	2: 125,143,678 (GRCm39)	L2817P	probably benign	Het
Fbxo42	T	A	4: 140,921,131 (GRCm39)		probably null	Het
Fbxo43	T	C	15: 36,162,358 (GRCm39)	D283G	probably damaging	Het
Fdxr	A	T	11: 115,167,656 (GRCm39)	S20T	probably benign	Het
Fkbp10	T	C	11: 100,312,048 (GRCm39)	Y203H	probably damaging	Het
Galntl6	C	T	8: 58,880,733 (GRCm39)		probably null	Het
Gask1b	G	A	3: 79,848,711 (GRCm39)	G486S	probably damaging	Het
Gm26657	C	A	4: 56,741,056 (GRCm39)	Y80*	probably null	Het
Gm973	T	A	1: 59,597,289 (GRCm39)	W440R	probably damaging	Het
Helz	T	A	11: 107,528,689 (GRCm39)	F903Y	probably damaging	Het
Iqcf4	T	A	9: 106,445,860 (GRCm39)	I96F	possibly damaging	Het
Kcnq5	T	A	1: 22,031,555 (GRCm39)	Q84L	probably benign	Het
Krit1	G	A	5: 3,862,772 (GRCm39)	G254S	probably damaging	Het
Krtap6-2	A	T	16: 89,216,510 (GRCm39)	Y152*	probably null	Het
Loricrin	A	T	3: 91,988,460 (GRCm39)	Y275*	probably null	Het
Map4	T	C	9: 109,863,720 (GRCm39)	I315T	probably benign	Het
Mcpt1	T	A	14: 56,256,609 (GRCm39)		probably null	Het
Mdh1b	C	A	1: 63,759,133 (GRCm39)	S153I	possibly damaging	Het
Mfsd12	C	A	10: 81,193,718 (GRCm39)	A96E	probably benign	Het
Mki67	T	C	7: 135,315,697 (GRCm39)	E55G	probably benign	Het
Mterf4	T	C	1: 93,232,689 (GRCm39)	E54G	probably benign	Het
Mucl3	A	G	17: 35,947,874 (GRCm39)	S452P	possibly damaging	Het
Nuggc	T	A	14: 65,882,490 (GRCm39)	I745N	probably damaging	Het
Or6k6	T	C	1: 173,945,115 (GRCm39)	I156V	probably benign	Het
Or7g18	A	G	9: 18,786,910 (GRCm39)	T93A	possibly damaging	Het
Parg	A	T	14: 31,930,758 (GRCm39)	N126I	possibly damaging	Het
Pcdh9	T	C	14: 94,123,987 (GRCm39)	I728V	possibly damaging	Het
Pcdha1	C	G	18: 37,065,511 (GRCm39)	P725R	probably benign	Het
Pcdha11	T	A	18: 37,145,849 (GRCm39)	L647M	probably damaging	Het
Phb2	T	C	6: 124,692,995 (GRCm39)		probably null	Het
Plb1	A	G	5: 32,483,610 (GRCm39)		probably null	Het
Pmch	T	A	10: 87,927,113 (GRCm39)	F39I	probably benign	Het
Psmd7	A	T	8: 108,307,920 (GRCm39)	S188T	possibly damaging	Het
Pycr2	T	A	1: 180,733,913 (GRCm39)	D168E	probably damaging	Het
Rab43	A	G	6: 87,771,692 (GRCm39)	Y96H	probably damaging	Het
Ralgapa2	C	T	2: 146,184,334 (GRCm39)	G1511S	probably benign	Het
Rb1cc1	T	A	1: 6,319,138 (GRCm39)	C852*	probably null	Het
Rexo2	T	C	9: 48,380,145 (GRCm39)	N229S	probably damaging	Het
Sema4f	A	G	6: 82,890,941 (GRCm39)	V622A	possibly damaging	Het
Serpina10	T	C	12: 103,594,806 (GRCm39)	S138G	probably benign	Het
Sptbn4	T	A	7: 27,061,059 (GRCm39)	T2415S	possibly damaging	Het
Tbc1d22b	A	G	17: 29,792,040 (GRCm39)	N257D	probably benign	Het
Tbl3	A	G	17: 24,921,205 (GRCm39)	F529L	probably damaging	Het
Them6	A	T	15: 74,593,427 (GRCm39)	N95I	probably benign	Het
Thrb	T	C	14: 18,008,608 (GRCm38)	S156P	possibly damaging	Het
Tmem186	G	A	16: 8,453,731 (GRCm39)	R177C	probably benign	Het
Ttn	T	A	2: 76,660,937 (GRCm39)		probably null	Het
Unc13a	T	A	8: 72,110,979 (GRCm39)	I411F	probably benign	Het
Uqcc1	T	C	2: 155,752,073 (GRCm39)		probably null	Het
Vmn1r56	T	C	7: 5,199,516 (GRCm39)	T34A	possibly damaging	Het
Vps39	A	T	2: 120,155,680 (GRCm39)	Y542*	probably null	Het
Wdr64	T	C	1: 175,556,542 (GRCm39)	L263P	probably damaging	Het
Wdr90	C	T	17: 26,065,300 (GRCm39)	R1652Q	probably damaging	Het
Wfdc16	A	G	2: 164,477,785 (GRCm39)	M88T	probably benign	Het
Wnk4	A	G	11: 101,160,403 (GRCm39)	D679G	probably damaging	Het
Ykt6	T	C	11: 5,912,751 (GRCm39)	I151T	probably benign	Het
Zbtb46	A	G	2: 181,033,225 (GRCm39)	C479R	probably damaging	Het
Zc3h7a	A	G	16: 10,967,115 (GRCm39)	I559T	probably benign	Het
Zfhx3	A	G	8: 109,678,283 (GRCm39)	N3111S	possibly damaging	Het
Zfp619	T	C	7: 39,184,432 (GRCm39)	V154A	possibly damaging	Het
Zfp629	A	T	7: 127,211,601 (GRCm39)	D69E	probably benign	Het
Zfp934	T	C	13: 62,666,358 (GRCm39)	H106R	possibly damaging	Het
Zfta	T	C	19: 7,399,737 (GRCm39)	S495P	probably damaging	Het
Zfyve9	G	A	4: 108,576,298 (GRCm39)	T261I	possibly damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	72,667,671 (GRCm39)	missense	probably benign
R0244:Zfp882	UTSW	8	72,667,367 (GRCm39)	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	72,668,459 (GRCm39)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	72,668,181 (GRCm39)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	72,668,530 (GRCm39)	nonsense	probably null
R1299:Zfp882	UTSW	8	72,667,317 (GRCm39)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	72,667,453 (GRCm39)	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	72,668,233 (GRCm39)	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	72,668,498 (GRCm39)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	72,668,204 (GRCm39)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	72,667,303 (GRCm39)	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	72,666,999 (GRCm39)	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	72,668,349 (GRCm39)	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	72,668,484 (GRCm39)	missense	probably damaging	1.00
R6888:Zfp882	UTSW	8	72,668,130 (GRCm39)	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	72,668,517 (GRCm39)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	72,667,093 (GRCm39)	critical splice donor site	probably null
R7793:Zfp882	UTSW	8	72,666,985 (GRCm39)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	72,667,962 (GRCm39)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	72,668,831 (GRCm39)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	72,667,915 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTTGAGGAAGGTTGAAAGTCATG -3'
(R):5'- CCCTGTGAATCTGTTCATGGTATC -3'

Sequencing Primer
(F):5'- GGTTGAAAGTCATGAACAAACTCAC -3'
(R):5'- GTGATGCATGAAGGCTTTCCCAC -3'

Posted On

2019-11-26