Mutagenetix > Incidental Mutation

Incidental Mutation 'R7781:Zfp1005'

599217

Institutional Source

Beutler Lab

Gene Symbol

Zfp1005

Ensembl Gene

ENSMUSG00000079008

Gene Name

zinc finger protein 1005

Synonyms

Gm10749, EG640962, Gm14124

MMRRC Submission

045837-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R7781 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150099437-150112220 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 150109577 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 89 (H89L)

Ref Sequence

ENSEMBL: ENSMUSP00000105548 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109922]

AlphaFold

A2AU83

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109922
AA Change: H89L

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000105548
Gene: ENSMUSG00000079008
AA Change: H89L

Domain	Start	End	E-Value	Type
KRAB	4	66	9.26e-19	SMART
ZnF_C2H2	103	125	7.49e-5	SMART
ZnF_C2H2	131	151	9.46e0	SMART
ZnF_C2H2	159	181	5.9e-3	SMART
ZnF_C2H2	187	209	6.67e-2	SMART
ZnF_C2H2	215	237	4.87e-4	SMART
ZnF_C2H2	243	265	2.82e0	SMART
ZnF_C2H2	271	293	2.2e2	SMART
ZnF_C2H2	299	321	1.4e-4	SMART
ZnF_C2H2	327	349	1.6e-4	SMART
ZnF_C2H2	355	377	1.18e-2	SMART
ZnF_C2H2	383	405	1.38e-3	SMART
ZnF_C2H2	411	433	9.56e1	SMART
ZnF_C2H2	439	461	6.99e-5	SMART
ZnF_C2H2	467	489	2.99e-4	SMART
ZnF_C2H2	495	517	7.78e-3	SMART
ZnF_C2H2	523	545	1.04e-3	SMART
ZnF_C2H2	551	573	1.6e-4	SMART
ZnF_C2H2	579	601	1.18e-2	SMART
ZnF_C2H2	607	629	4.54e-4	SMART
ZnF_C2H2	635	657	4.24e-4	SMART
ZnF_C2H2	663	685	1.2e-3	SMART
ZnF_C2H2	691	713	8.47e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700064H15Rik	G	A	3: 19,682,706 (GRCm39)	P23S	unknown	Het
Abcc6	C	T	7: 45,655,030 (GRCm39)	R487Q	probably damaging	Het
Abl1	A	T	2: 31,680,709 (GRCm39)	T334S	probably damaging	Het
Adam6b	T	C	12: 113,454,962 (GRCm39)	F593S	probably damaging	Het
Alx1	A	T	10: 102,845,053 (GRCm39)	M326K	probably damaging	Het
Ankhd1	A	T	18: 36,758,258 (GRCm39)	D984V	probably damaging	Het
Asb15	A	G	6: 24,562,644 (GRCm39)	N202S	probably benign	Het
Ate1	A	T	7: 130,121,157 (GRCm39)	V12E	probably damaging	Het
Atl2	A	T	17: 80,167,260 (GRCm39)	Y254N	probably damaging	Het
Atp13a5	A	G	16: 29,116,226 (GRCm39)	M630T	probably benign	Het
Atp5po	A	T	16: 91,723,417 (GRCm39)	I124N	possibly damaging	Het
BC005537	C	T	13: 24,987,382 (GRCm39)	R7W	possibly damaging	Het
Bicdl1	T	C	5: 115,799,546 (GRCm39)	E181G	probably damaging	Het
Bnipl	C	A	3: 95,151,486 (GRCm39)	W272L	probably damaging	Het
Catsperd	A	T	17: 56,971,072 (GRCm39)	H712L	probably benign	Het
Cilp2	T	C	8: 70,334,997 (GRCm39)	D667G	possibly damaging	Het
Cntn4	A	C	6: 106,500,575 (GRCm39)	K351Q	probably damaging	Het
Coq7	A	T	7: 118,125,111 (GRCm39)	I171N	probably damaging	Het
Csf2rb	T	A	15: 78,228,771 (GRCm39)	F371I	probably benign	Het
Cyp3a13	C	T	5: 137,897,136 (GRCm39)	V393M	possibly damaging	Het
Dennd2a	A	G	6: 39,470,000 (GRCm39)	V564A	probably damaging	Het
Ear14	T	C	14: 51,441,468 (GRCm39)	L108P	probably damaging	Het
Egflam	C	A	15: 7,283,227 (GRCm39)	V277F	probably null	Het
Epb42	T	G	2: 120,864,916 (GRCm39)	K58N	probably benign	Het
Faap100	T	C	11: 120,265,089 (GRCm39)	M596V	probably benign	Het
Fbxw15	T	A	9: 109,386,330 (GRCm39)	T270S	possibly damaging	Het
Gabpb1	C	T	2: 126,481,120 (GRCm39)	C342Y	possibly damaging	Het
Gls	A	T	1: 52,251,492 (GRCm39)	C288*	probably null	Het
Grm8	G	T	6: 27,285,786 (GRCm39)	D875E	probably benign	Het
Hdac4	C	A	1: 91,903,387 (GRCm39)	R514L	probably benign	Het
Hps4	T	A	5: 112,518,388 (GRCm39)	N460K	probably benign	Het
Inpp5d	T	C	1: 87,627,394 (GRCm39)	F565S	probably damaging	Het
Kcnh5	C	A	12: 75,023,455 (GRCm39)	V538F	probably damaging	Het
Kcp	T	C	6: 29,497,764 (GRCm39)	N499S	probably damaging	Het
Klk15	T	C	7: 43,588,980 (GRCm39)	L244S	probably benign	Het
Lamp3	A	T	16: 19,518,440 (GRCm39)	S266T	possibly damaging	Het
Lemd3	A	G	10: 120,761,678 (GRCm39)	F863L	probably damaging	Het
Mga	T	A	2: 119,747,838 (GRCm39)	V663D	probably damaging	Het
Naa15	A	G	3: 51,378,904 (GRCm39)		probably null	Het
Ndrg3	C	A	2: 156,770,733 (GRCm39)	G352*	probably null	Het
Nr2f6	G	T	8: 71,828,595 (GRCm39)	N233K	possibly damaging	Het
Or4a79	A	G	2: 89,551,879 (GRCm39)	F192S	probably benign	Het
Plxnb2	T	A	15: 89,041,225 (GRCm39)	M1774L	possibly damaging	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Rpl23a	G	A	11: 78,073,654 (GRCm39)	R62W	probably benign	Het
Rubcnl	A	G	14: 75,269,530 (GRCm39)	R63G	probably damaging	Het
Ryr1	T	C	7: 28,767,055 (GRCm39)	D2976G	probably damaging	Het
Siglec1	A	G	2: 130,923,258 (GRCm39)	Y496H	probably damaging	Het
Sipa1l2	A	G	8: 126,218,566 (GRCm39)	V257A	possibly damaging	Het
Slc24a4	T	C	12: 102,201,112 (GRCm39)		probably null	Het
Slc4a1ap	T	C	5: 31,684,822 (GRCm39)	S153P	probably damaging	Het
Slc4a4	A	T	5: 89,376,791 (GRCm39)	E1006V	possibly damaging	Het
Svep1	T	G	4: 58,069,251 (GRCm39)	E2845A	possibly damaging	Het
Tlr1	T	C	5: 65,084,079 (GRCm39)	N166S	possibly damaging	Het
Tmem231	C	T	8: 112,644,922 (GRCm39)		probably null	Het
Tsc2	C	A	17: 24,827,089 (GRCm39)	L873F	possibly damaging	Het
Unc13b	T	G	4: 43,259,546 (GRCm39)	S1403A	possibly damaging	Het
Vmn1r7	A	G	6: 57,001,553 (GRCm39)	F236L	probably benign	Het
Vmn2r111	T	C	17: 22,789,714 (GRCm39)	S431G	probably benign	Het
Wdr7	A	T	18: 63,910,860 (GRCm39)	K751*	probably null	Het
Wdr90	C	T	17: 26,065,300 (GRCm39)	R1652Q	probably damaging	Het
Zcchc2	T	C	1: 105,931,895 (GRCm39)	Y366H	probably damaging	Het
Zfp607a	C	T	7: 27,565,000 (GRCm39)	R56C	possibly damaging	Het

Other mutations in Zfp1005

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01550:Zfp1005	APN	2	150,108,363 (GRCm39)	splice site	probably benign
R0220:Zfp1005	UTSW	2	150,110,595 (GRCm39)	missense	unknown
R0396:Zfp1005	UTSW	2	150,109,973 (GRCm39)	missense	probably damaging	1.00
R0402:Zfp1005	UTSW	2	150,111,136 (GRCm39)	missense	possibly damaging	0.93
R0446:Zfp1005	UTSW	2	150,109,993 (GRCm39)	missense	possibly damaging	0.71
R0462:Zfp1005	UTSW	2	150,111,122 (GRCm39)	missense	possibly damaging	0.80
R0507:Zfp1005	UTSW	2	150,110,044 (GRCm39)	missense	possibly damaging	0.69
R0605:Zfp1005	UTSW	2	150,110,523 (GRCm39)	missense	unknown
R0838:Zfp1005	UTSW	2	150,111,220 (GRCm39)	missense	possibly damaging	0.74
R1327:Zfp1005	UTSW	2	150,108,070 (GRCm39)	missense	possibly damaging	0.71
R1405:Zfp1005	UTSW	2	150,109,620 (GRCm39)	nonsense	probably null
R1405:Zfp1005	UTSW	2	150,109,620 (GRCm39)	nonsense	probably null
R2114:Zfp1005	UTSW	2	150,109,819 (GRCm39)	missense	unknown
R2140:Zfp1005	UTSW	2	150,111,281 (GRCm39)	missense	probably benign	0.33
R3683:Zfp1005	UTSW	2	150,109,976 (GRCm39)	missense	probably benign	0.41
R3917:Zfp1005	UTSW	2	150,108,039 (GRCm39)	splice site	probably benign
R4084:Zfp1005	UTSW	2	150,108,122 (GRCm39)	missense	possibly damaging	0.85
R4499:Zfp1005	UTSW	2	150,111,362 (GRCm39)	missense	possibly damaging	0.93
R4683:Zfp1005	UTSW	2	150,108,390 (GRCm39)	missense	possibly damaging	0.53
R4762:Zfp1005	UTSW	2	150,109,549 (GRCm39)	missense	possibly damaging	0.53
R4937:Zfp1005	UTSW	2	150,110,680 (GRCm39)	missense	unknown
R5678:Zfp1005	UTSW	2	150,110,425 (GRCm39)	nonsense	probably null
R5696:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5697:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5698:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5769:Zfp1005	UTSW	2	150,110,198 (GRCm39)	missense	possibly damaging	0.87
R5780:Zfp1005	UTSW	2	150,108,139 (GRCm39)	missense	probably benign	0.05
R5973:Zfp1005	UTSW	2	150,109,855 (GRCm39)	missense	unknown
R6662:Zfp1005	UTSW	2	150,108,172 (GRCm39)	critical splice donor site	probably null
R6878:Zfp1005	UTSW	2	150,108,406 (GRCm39)	missense	possibly damaging	0.86
R7037:Zfp1005	UTSW	2	150,108,376 (GRCm39)	missense	possibly damaging	0.86
R7081:Zfp1005	UTSW	2	150,110,189 (GRCm39)	missense	possibly damaging	0.66
R7413:Zfp1005	UTSW	2	150,108,081 (GRCm39)	missense	possibly damaging	0.93
R7725:Zfp1005	UTSW	2	150,110,468 (GRCm39)	missense	unknown
R8197:Zfp1005	UTSW	2	150,109,577 (GRCm39)	missense	possibly damaging	0.53
R8355:Zfp1005	UTSW	2	150,109,876 (GRCm39)	missense	unknown
R8517:Zfp1005	UTSW	2	150,110,043 (GRCm39)	missense	probably benign	0.33
R8812:Zfp1005	UTSW	2	150,109,624 (GRCm39)	missense	possibly damaging	0.83
R9108:Zfp1005	UTSW	2	150,109,969 (GRCm39)	missense	possibly damaging	0.61
R9488:Zfp1005	UTSW	2	150,110,477 (GRCm39)	missense	unknown
R9499:Zfp1005	UTSW	2	150,109,856 (GRCm39)	missense	unknown
R9551:Zfp1005	UTSW	2	150,109,856 (GRCm39)	missense	unknown
R9567:Zfp1005	UTSW	2	150,109,517 (GRCm39)	missense	possibly damaging	0.53
R9646:Zfp1005	UTSW	2	150,110,104 (GRCm39)	missense	probably benign	0.43
R9709:Zfp1005	UTSW	2	150,110,305 (GRCm39)	missense	possibly damaging	0.47
R9719:Zfp1005	UTSW	2	150,111,304 (GRCm39)	missense	possibly damaging	0.74
R9779:Zfp1005	UTSW	2	150,108,064 (GRCm39)	missense	possibly damaging	0.92
X0022:Zfp1005	UTSW	2	150,109,578 (GRCm39)	missense	possibly damaging	0.53
Z1177:Zfp1005	UTSW	2	150,110,244 (GRCm39)	missense	possibly damaging	0.94
Z1177:Zfp1005	UTSW	2	150,110,237 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- CAAAACCACCCATAATGAAGGGG -3'
(R):5'- TTTATGATACAGGAGACCACTTTGACC -3'

Sequencing Primer
(F):5'- CCCATAATGAAGGGGAACTCCATG -3'
(R):5'- TATGTCCTCGGAGACCAGTTGAAC -3'

Posted On

2019-11-26