Mutagenetix > Incidental Mutation

Incidental Mutation 'R7783:Try4'

599372

Institutional Source

Beutler Lab

Gene Symbol

Try4

Ensembl Gene

ENSMUSG00000054106

Gene Name

trypsin 4

Synonyms

0910001B19Rik, Td

MMRRC Submission

045839-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7783 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41302269-41305532 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 41302295 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 4 (L4P)

Ref Sequence

ENSEMBL: ENSMUSP00000031913 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031913]

AlphaFold

Q9R0T7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000031913
AA Change: L4P

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000031913
Gene: ENSMUSG00000054106
AA Change: L4P

Domain	Start	End	E-Value	Type
low complexity region	3	16	N/A	INTRINSIC
Tryp_SPc	23	239	9.72e-105	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb9	G	T	5: 124,078,812 (GRCm38)	Y447*	probably null	Het
Abl2	T	C	1: 156,559,071 (GRCm38)	V8A	probably benign	Het
Adam33	C	T	2: 131,058,337 (GRCm38)	R103K	unknown	Het
Adamts13	G	T	2: 26,990,585 (GRCm38)	A727S	not run	Het
Alpk2	A	T	18: 65,306,254 (GRCm38)	C689*	probably null	Het
Amt	C	T	9: 108,297,215 (GRCm38)	Q60*	probably null	Het
Ankrd12	A	G	17: 66,027,250 (GRCm38)		probably null	Het
Ankrd28	A	T	14: 31,706,813 (GRCm38)	N920K	probably damaging	Het
Ankrd36	T	C	11: 5,635,359 (GRCm38)	L390P	probably damaging	Het
Arvcf	T	G	16: 18,389,198 (GRCm38)	H7Q	probably benign	Het
Asns	A	G	6: 7,677,978 (GRCm38)	S367P	probably damaging	Het
BC005537	C	T	13: 24,803,399 (GRCm38)	R7W	possibly damaging	Het
C7	T	A	15: 5,007,710 (GRCm38)	H562L	probably benign	Het
Ccdc77	T	C	6: 120,350,373 (GRCm38)	D37G	probably damaging	Het
Cdc14a	C	T	3: 116,404,587 (GRCm38)	A58T	probably damaging	Het
Cdc42bpb	C	T	12: 111,336,025 (GRCm38)		probably null	Het
Corin	A	T	5: 72,301,624 (GRCm38)	F1068L	probably benign	Het
Epb42	T	G	2: 121,034,435 (GRCm38)	K58N	probably benign	Het
Ercc3	T	A	18: 32,248,243 (GRCm38)	S371T	probably damaging	Het
Fam193a	A	C	5: 34,431,180 (GRCm38)	K358Q	probably damaging	Het
Fem1a	G	A	17: 56,257,522 (GRCm38)	C205Y	probably benign	Het
Fh1	G	A	1: 175,612,178 (GRCm38)	T233M	probably damaging	Het
Ftsj3	CCTTCTTCTTCTTCTTCT	CCTTCTTCTTCTTCT	11: 106,252,551 (GRCm38)		probably benign	Het
Gabra6	T	C	11: 42,316,462 (GRCm38)	N265S	probably damaging	Het
Gm10801	C	CGTG	2: 98,663,807 (GRCm38)		probably null	Het
Gm996	A	T	2: 25,577,808 (GRCm38)	L697Q	probably damaging	Het
Grm6	T	A	11: 50,863,082 (GRCm38)	C738S	probably damaging	Het
Gtsf1	C	T	15: 103,428,569 (GRCm38)		probably benign	Het
Hcrtr2	T	A	9: 76,232,914 (GRCm38)	Y364F	probably damaging	Het
Ick	G	T	9: 78,135,645 (GRCm38)	V51F	probably damaging	Het
Ifit1bl1	T	C	19: 34,593,936 (GRCm38)	I374V	probably benign	Het
Il31ra	A	T	13: 112,541,251 (GRCm38)	F250L	probably benign	Het
Iqgap1	A	G	7: 80,809,059 (GRCm38)	V37A	probably benign	Het
Izumo3	A	T	4: 92,145,023 (GRCm38)	I182K	probably damaging	Het
Kidins220	G	A	12: 24,988,556 (GRCm38)	A36T	probably damaging	Het
Lrrtm1	G	T	6: 77,244,253 (GRCm38)	R231L	probably damaging	Het
Micalcl	A	T	7: 112,412,976 (GRCm38)	S678C	probably damaging	Het
Mme	T	G	3: 63,364,867 (GRCm38)	F629C	probably damaging	Het
Muc5b	T	A	7: 141,857,341 (GRCm38)	H1341Q	unknown	Het
Olfr1339	T	A	4: 118,734,902 (GRCm38)	D124E	probably damaging	Het
Olfr1391	A	G	11: 49,328,202 (GRCm38)	S264G	probably benign	Het
Olfr495	A	G	7: 108,396,089 (GRCm38)	H323R	probably benign	Het
Olfr513	A	T	7: 108,755,569 (GRCm38)	T238S	probably damaging	Het
Olfr61	A	G	7: 140,637,724 (GRCm38)	T8A	possibly damaging	Het
Parm1	A	T	5: 91,593,865 (GRCm38)	M31L	probably benign	Het
Pcdhb18	G	A	18: 37,489,821 (GRCm38)	C68Y	probably benign	Het
Pkn3	A	T	2: 30,079,622 (GRCm38)	E35V	probably damaging	Het
Pla2g4a	A	T	1: 149,872,744 (GRCm38)	Y238N	probably damaging	Het
Pld6	T	C	11: 59,787,271 (GRCm38)	D122G	probably damaging	Het
Prag1	G	T	8: 36,103,255 (GRCm38)	A331S	possibly damaging	Het
Rbm44	A	G	1: 91,168,829 (GRCm38)	D970G	probably benign	Het
Rps6kc1	A	G	1: 190,773,654 (GRCm38)	V1037A	probably benign	Het
Slc12a7	T	C	13: 73,805,469 (GRCm38)	V766A	probably benign	Het
Spata18	A	G	5: 73,668,610 (GRCm38)	T87A		Het
St3gal3	T	C	4: 117,940,123 (GRCm38)	M308V	probably benign	Het
Stx19	T	C	16: 62,822,286 (GRCm38)	L155S	probably benign	Het
Tespa1	A	T	10: 130,356,883 (GRCm38)	T145S	probably damaging	Het
Timm21	A	C	18: 84,947,721 (GRCm38)	F221V	possibly damaging	Het
Tlr1	A	T	5: 64,924,921 (GRCm38)	F771Y	probably damaging	Het
Tmem150a	C	A	6: 72,358,623 (GRCm38)	L125I	unknown	Het
Txlna	C	T	4: 129,632,157 (GRCm38)	R299H	probably damaging	Het
Txndc16	A	T	14: 45,144,960 (GRCm38)	N609K	probably benign	Het
Upf1	T	C	8: 70,352,858 (GRCm38)	T46A	probably benign	Het
Zfp266	T	C	9: 20,500,330 (GRCm38)	N184D	probably benign	Het
Zfp407	A	G	18: 84,209,922 (GRCm38)	V1854A	possibly damaging	Het
Zfp626	T	A	7: 27,818,370 (GRCm38)	C259S	possibly damaging	Het

Other mutations in Try4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01773:Try4	APN	6	41,305,026 (GRCm38)	missense	probably damaging	1.00
IGL02216:Try4	APN	6	41,305,031 (GRCm38)	missense	probably benign
R0537:Try4	UTSW	6	41,304,362 (GRCm38)	missense	probably benign
R0731:Try4	UTSW	6	41,304,367 (GRCm38)	missense	probably benign	0.01
R1113:Try4	UTSW	6	41,305,374 (GRCm38)	missense	possibly damaging	0.90
R1833:Try4	UTSW	6	41,303,431 (GRCm38)	missense	probably damaging	0.98
R2246:Try4	UTSW	6	41,305,472 (GRCm38)	missense	possibly damaging	0.80
R4131:Try4	UTSW	6	41,305,401 (GRCm38)	nonsense	probably null
R4414:Try4	UTSW	6	41,304,971 (GRCm38)	missense	possibly damaging	0.84
R5457:Try4	UTSW	6	41,303,421 (GRCm38)	missense	probably damaging	1.00
R5707:Try4	UTSW	6	41,305,043 (GRCm38)	missense	possibly damaging	0.65
R6023:Try4	UTSW	6	41,303,421 (GRCm38)	missense	probably damaging	1.00
R7131:Try4	UTSW	6	41,304,403 (GRCm38)	missense	probably benign	0.03
R8051:Try4	UTSW	6	41,305,062 (GRCm38)	missense	probably damaging	0.99
R9320:Try4	UTSW	6	41,305,074 (GRCm38)	critical splice donor site	probably null
R9730:Try4	UTSW	6	41,305,062 (GRCm38)	missense	probably damaging	1.00
RF007:Try4	UTSW	6	41,305,363 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTGCACCCGGAGTAAATTTCC -3'
(R):5'- TAGGCTTGCAGAGAAGCTTG -3'

Sequencing Primer
(F):5'- GCACCCGGAGTAAATTTCCTTATCAC -3'
(R):5'- CTTGCAGAGAAGCTTGAAAGATTG -3'

Posted On

2019-11-26