Mutagenetix > Incidental Mutation

Incidental Mutation 'R7784:Fbxw25'

599447

Institutional Source

Beutler Lab

Gene Symbol

Fbxw25

Ensembl Gene

ENSMUSG00000094992

Gene Name

F-box and WD-40 domain protein 25

Synonyms

E330001B16Rik

MMRRC Submission

045840-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R7784 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109645122-109664652 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 109650119 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 355 (D355N)

Ref Sequence

ENSEMBL: ENSMUSP00000128652 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163839]

AlphaFold

F7C9P2

Predicted Effect

SMART Domains

Protein: ENSMUSP00000128652
Gene: ENSMUSG00000094992
AA Change: D355N

Domain	Start	End	E-Value	Type
FBOX	5	45	5.44e-6	SMART
SCOP:d1gxra_	119	228	1e-6	SMART
Blast:WD40	137	176	6e-6	BLAST

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001J03Rik	A	T	5: 146,182,828		probably null	Het
3425401B19Rik	A	T	14: 32,659,840	S1389R	probably benign	Het
Abca9	A	T	11: 110,154,417	C363*	probably null	Het
Actbl2	T	A	13: 111,255,411	N93K	probably damaging	Het
Adamtsl3	T	C	7: 82,573,989	Y993H	probably damaging	Het
Adgrg1	G	A	8: 95,012,882	W653*	probably null	Het
Akap13	T	A	7: 75,610,328	V97D	probably benign	Het
C130026I21Rik	T	A	1: 85,212,474		probably null	Het
Cacna1d	T	A	14: 30,123,439	D613V	probably damaging	Het
Col10a1	C	A	10: 34,394,218	P62H	unknown	Het
Cpb2	A	T	14: 75,275,040	N298Y	probably damaging	Het
Ddc	A	G	11: 11,839,396		probably null	Het
Ddx6	T	C	9: 44,630,142		probably null	Het
Epb42	T	G	2: 121,034,435	K58N	probably benign	Het
Eps8	T	A	6: 137,499,587	I605L	probably benign	Het
Eps8l1	T	C	7: 4,472,122	L304P	probably damaging	Het
Erbb4	T	A	1: 68,075,499	I929F	probably damaging	Het
Erc2	A	T	14: 27,898,594	N393I	probably damaging	Het
Ffar2	T	C	7: 30,819,258	K286E	probably benign	Het
Gabrd	A	G	4: 155,388,932		probably null	Het
Ganc	G	A	2: 120,436,668	W488*	probably null	Het
Gm5724	C	T	6: 141,713,193		probably null	Het
Ifi207	T	A	1: 173,730,132	M347L	unknown	Het
Kat6b	A	T	14: 21,660,841	I619F	probably damaging	Het
Kif26a	A	G	12: 112,178,147	R1612G	possibly damaging	Het
Kifc3	A	G	8: 95,110,692		probably null	Het
Krt39	A	T	11: 99,521,031	C76*	probably null	Het
Lcmt1	G	T	7: 123,401,495	R84L	probably benign	Het
Lrit1	A	G	14: 37,061,780	Y355C	probably benign	Het
Mad2l1	C	A	6: 66,535,413		probably null	Het
Med23	C	T	10: 24,902,448	T870M	probably damaging	Het
Mrpl2	A	G	17: 46,648,591		probably null	Het
Mtmr6	A	G	14: 60,300,445	D593G	probably benign	Het
Myo15b	G	A	11: 115,861,340	V683M		Het
Neb	T	A	2: 52,235,488	M506L		Het
Olfr103	A	G	17: 37,337,055	F59S	probably damaging	Het
Olfr103	T	C	17: 37,336,578	Y218C	probably benign	Het
Olfr1154	G	A	2: 87,903,193	T161I	probably benign	Het
Olfr434	T	A	6: 43,217,388	H158Q	possibly damaging	Het
Pdzd8	A	G	19: 59,327,863	F294L	probably damaging	Het
Rabgap1	G	A	2: 37,487,532	S347N	possibly damaging	Het
Rasgrf2	T	C	13: 91,896,082	T350A		Het
Rbp3	A	T	14: 33,954,158	H21L	probably benign	Het
Rp1	C	A	1: 4,142,658	V1069F	unknown	Het
Rtn4	T	A	11: 29,741,048	L1113*	probably null	Het
Ryr3	A	G	2: 112,775,695	F2407L	probably damaging	Het
Sept2	T	A	1: 93,497,444	D107E	probably damaging	Het
Sept4	A	G	11: 87,579,008	T7A	probably benign	Het
Slc34a3	T	A	2: 25,232,225	I123F	probably damaging	Het
Slc9a4	T	C	1: 40,600,776	Y243H	probably damaging	Het
Slco1a1	T	A	6: 141,943,388	E66V	probably damaging	Het
Spata33	A	G	8: 123,213,252	R68G	unknown	Het
Spta1	G	A	1: 174,202,451	D928N	probably damaging	Het
St8sia5	A	G	18: 77,254,550	S319G	probably benign	Het
Tmem208	A	G	8: 105,328,833	D149G	possibly damaging	Het
Trank1	A	T	9: 111,364,103	I583F	probably damaging	Het
Trio	C	T	15: 27,763,994	V2015M	probably damaging	Het
Tsc22d1	C	T	14: 76,416,701	Q207*	probably null	Het
Tshr	A	G	12: 91,505,305	D143G	probably benign	Het
Txlna	C	T	4: 129,632,157	R299H	probably damaging	Het
Ush2a	A	T	1: 188,444,592	T1318S	possibly damaging	Het
Utp14b	A	G	1: 78,664,943	K186R	probably damaging	Het
Vars2	C	T	17: 35,658,158	A884T	possibly damaging	Het
Zfp354c	TCACACTCGGCACA	TCACA	11: 50,815,240		probably benign	Het
Zfp775	A	G	6: 48,619,249	Q19R	possibly damaging	Het

Other mutations in Fbxw25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03330:Fbxw25	APN	9	109645239	missense	probably benign	0.00
doughnuts	UTSW	9	109650064	missense
goodtimes	UTSW	9	109663374	critical splice donor site	probably null
shakeys	UTSW	9	109654583	missense
R0158:Fbxw25	UTSW	9	109654652	missense	possibly damaging	0.74
R0850:Fbxw25	UTSW	9	109649617	missense	probably benign
R1109:Fbxw25	UTSW	9	109650060	missense	probably benign	0.00
R1386:Fbxw25	UTSW	9	109654641	missense	possibly damaging	0.77
R1609:Fbxw25	UTSW	9	109663510	missense	probably benign	0.11
R1750:Fbxw25	UTSW	9	109650073	missense	probably benign	0.23
R1977:Fbxw25	UTSW	9	109652856	missense	possibly damaging	0.72
R2427:Fbxw25	UTSW	9	109652860	missense	probably benign	0.09
R3841:Fbxw25	UTSW	9	109662134	nonsense	probably null
R4356:Fbxw25	UTSW	9	109662085	missense	probably damaging	1.00
R4934:Fbxw25	UTSW	9	109651637	missense	possibly damaging	0.63
R5024:Fbxw25	UTSW	9	109663374	critical splice donor site	probably null
R5175:Fbxw25	UTSW	9	109664563	missense	probably damaging	1.00
R5323:Fbxw25	UTSW	9	109663505	missense	probably benign	0.04
R5389:Fbxw25	UTSW	9	109652886	missense	possibly damaging	0.95
R5493:Fbxw25	UTSW	9	109652916	missense	probably benign	0.01
R6268:Fbxw25	UTSW	9	109654650	missense	probably damaging	1.00
R6739:Fbxw25	UTSW	9	109651631	missense	probably benign	0.29
R7275:Fbxw25	UTSW	9	109654592	missense
R7492:Fbxw25	UTSW	9	109664530	critical splice donor site	probably null
R7623:Fbxw25	UTSW	9	109654583	missense
R7861:Fbxw25	UTSW	9	109664557	nonsense	probably null
R7887:Fbxw25	UTSW	9	109649594	critical splice donor site	probably null
R8973:Fbxw25	UTSW	9	109650064	missense
R9517:Fbxw25	UTSW	9	109651824	missense
R9563:Fbxw25	UTSW	9	109654608	missense
R9565:Fbxw25	UTSW	9	109654608	missense
X0023:Fbxw25	UTSW	9	109651775	missense	possibly damaging	0.94
Z1176:Fbxw25	UTSW	9	109651738	missense

Predicted Primers

PCR Primer
(F):5'- TAGGCTGGAGTCAATGTCAGAC -3'
(R):5'- CTAGCATGCCATGGAAGGAG -3'

Sequencing Primer
(F):5'- AGTCTGCCTGTGAACTCTGCAAG -3'
(R):5'- CCATGGAAGGAGGGATATGCTTTTC -3'

Posted On

2019-11-26