Incidental Mutation 'R7784:Rasgrf2'
ID 599460
Institutional Source Beutler Lab
Gene Symbol Rasgrf2
Ensembl Gene ENSMUSG00000021708
Gene Name RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms Grf2, 6330417G04Rik
MMRRC Submission 045840-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.191) question?
Stock # R7784 (G1)
Quality Score 225.009
Status Validated
Chromosome 13
Chromosomal Location 92028519-92268164 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 92044201 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 350 (T350A)
Gene Model predicted gene model for transcript(s): [ENSMUST00000099326]
AlphaFold P70392
Predicted Effect probably benign
Transcript: ENSMUST00000099326
AA Change: T951A

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)
SMART Domains Protein: ENSMUSP00000096930
Gene: ENSMUSG00000021708
AA Change: T951A

DomainStartEndE-ValueType
PH 23 135 1.29e-16 SMART
IQ 204 226 1.3e0 SMART
RhoGEF 247 428 2.2e-51 SMART
RasGEFN 633 775 9.35e-15 SMART
RasGEFN 786 923 6.04e-9 SMART
RasGEF 949 1186 2.97e-112 SMART
Predicted Effect
SMART Domains Protein: ENSMUSP00000116892
Gene: ENSMUSG00000021708
AA Change: T350A

DomainStartEndE-ValueType
RasGEFN 33 175 9.35e-15 SMART
RasGEFN 186 323 6.04e-9 SMART
RasGEF 349 586 2.97e-112 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.3%
Validation Efficiency 100% (66/66)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for a targeted null mutation exhibit decreased Il2 and TNF-alpha production in stimulated T cells. Mice homozygous for mutations in both Rasgrf1 and Rasgrf2 exhibit no additional abnormalities than those observed in the Rasgrf1 mutant mice. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700001J03Rik A T 5: 146,119,638 (GRCm39) probably null Het
3425401B19Rik A T 14: 32,381,797 (GRCm39) S1389R probably benign Het
Abca9 A T 11: 110,045,243 (GRCm39) C363* probably null Het
Actbl2 T A 13: 111,391,945 (GRCm39) N93K probably damaging Het
Adamtsl3 T C 7: 82,223,197 (GRCm39) Y993H probably damaging Het
Adgrg1 G A 8: 95,739,510 (GRCm39) W653* probably null Het
Akap13 T A 7: 75,260,076 (GRCm39) V97D probably benign Het
Cacna1d T A 14: 29,845,396 (GRCm39) D613V probably damaging Het
Col10a1 C A 10: 34,270,214 (GRCm39) P62H unknown Het
Cpb2 A T 14: 75,512,480 (GRCm39) N298Y probably damaging Het
Ddc A G 11: 11,789,396 (GRCm39) probably null Het
Ddx6 T C 9: 44,541,439 (GRCm39) probably null Het
Epb42 T G 2: 120,864,916 (GRCm39) K58N probably benign Het
Eps8 T A 6: 137,476,585 (GRCm39) I605L probably benign Het
Eps8l1 T C 7: 4,475,121 (GRCm39) L304P probably damaging Het
Erbb4 T A 1: 68,114,658 (GRCm39) I929F probably damaging Het
Erc2 A T 14: 27,620,551 (GRCm39) N393I probably damaging Het
Fbxw25 C T 9: 109,479,187 (GRCm39) D355N Het
Ffar2 T C 7: 30,518,683 (GRCm39) K286E probably benign Het
Gabrd A G 4: 155,473,389 (GRCm39) probably null Het
Ganc G A 2: 120,267,149 (GRCm39) W488* probably null Het
Ifi207 T A 1: 173,557,698 (GRCm39) M347L unknown Het
Kat6b A T 14: 21,710,909 (GRCm39) I619F probably damaging Het
Kif26a A G 12: 112,144,581 (GRCm39) R1612G possibly damaging Het
Kifc3 A G 8: 95,837,320 (GRCm39) probably null Het
Krt39 A T 11: 99,411,857 (GRCm39) C76* probably null Het
Lcmt1 G T 7: 123,000,718 (GRCm39) R84L probably benign Het
Lrit1 A G 14: 36,783,737 (GRCm39) Y355C probably benign Het
Mad2l1 C A 6: 66,512,397 (GRCm39) probably null Het
Med23 C T 10: 24,778,346 (GRCm39) T870M probably damaging Het
Mrpl2 A G 17: 46,959,517 (GRCm39) probably null Het
Mtmr6 A G 14: 60,537,894 (GRCm39) D593G probably benign Het
Myo15b G A 11: 115,752,166 (GRCm39) V683M Het
Neb T A 2: 52,125,500 (GRCm39) M506L Het
Or12d13 T C 17: 37,647,469 (GRCm39) Y218C probably benign Het
Or12d13 A G 17: 37,647,946 (GRCm39) F59S probably damaging Het
Or2a20 T A 6: 43,194,322 (GRCm39) H158Q possibly damaging Het
Or9m1 G A 2: 87,733,537 (GRCm39) T161I probably benign Het
Pdzd8 A G 19: 59,316,295 (GRCm39) F294L probably damaging Het
Rabgap1 G A 2: 37,377,544 (GRCm39) S347N possibly damaging Het
Rbp3 A T 14: 33,676,115 (GRCm39) H21L probably benign Het
Rp1 C A 1: 4,212,881 (GRCm39) V1069F unknown Het
Rtn4 T A 11: 29,691,048 (GRCm39) L1113* probably null Het
Ryr3 A G 2: 112,606,040 (GRCm39) F2407L probably damaging Het
Septin2 T A 1: 93,425,166 (GRCm39) D107E probably damaging Het
Septin4 A G 11: 87,469,834 (GRCm39) T7A probably benign Het
Slc34a3 T A 2: 25,122,237 (GRCm39) I123F probably damaging Het
Slc9a4 T C 1: 40,639,936 (GRCm39) Y243H probably damaging Het
Slco1a1 T A 6: 141,889,114 (GRCm39) E66V probably damaging Het
Slco1a7 C T 6: 141,658,919 (GRCm39) probably null Het
Sp140l2 T A 1: 85,190,195 (GRCm39) probably null Het
Spata33 A G 8: 123,939,991 (GRCm39) R68G unknown Het
Spta1 G A 1: 174,030,017 (GRCm39) D928N probably damaging Het
St8sia5 A G 18: 77,342,246 (GRCm39) S319G probably benign Het
Tmem208 A G 8: 106,055,465 (GRCm39) D149G possibly damaging Het
Trank1 A T 9: 111,193,171 (GRCm39) I583F probably damaging Het
Trio C T 15: 27,764,080 (GRCm39) V2015M probably damaging Het
Tsc22d1 C T 14: 76,654,141 (GRCm39) Q207* probably null Het
Tshr A G 12: 91,472,079 (GRCm39) D143G probably benign Het
Txlna C T 4: 129,525,950 (GRCm39) R299H probably damaging Het
Ush2a A T 1: 188,176,789 (GRCm39) T1318S possibly damaging Het
Utp14b A G 1: 78,642,660 (GRCm39) K186R probably damaging Het
Vars2 C T 17: 35,969,050 (GRCm39) A884T possibly damaging Het
Zfp354c TCACACTCGGCACA TCACA 11: 50,706,067 (GRCm39) probably benign Het
Zfp775 A G 6: 48,596,183 (GRCm39) Q19R possibly damaging Het
Other mutations in Rasgrf2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01308:Rasgrf2 APN 13 92,159,425 (GRCm39) splice site probably benign
IGL01358:Rasgrf2 APN 13 92,130,749 (GRCm39) missense probably benign 0.23
IGL01666:Rasgrf2 APN 13 92,174,718 (GRCm39) missense probably damaging 1.00
IGL01930:Rasgrf2 APN 13 92,130,857 (GRCm39) missense probably damaging 0.98
IGL02230:Rasgrf2 APN 13 92,136,145 (GRCm39) missense probably damaging 1.00
IGL02630:Rasgrf2 APN 13 92,267,900 (GRCm39) missense probably damaging 1.00
IGL02690:Rasgrf2 APN 13 92,167,273 (GRCm39) missense probably damaging 1.00
IGL02943:Rasgrf2 APN 13 92,131,752 (GRCm39) missense probably damaging 1.00
IGL03067:Rasgrf2 APN 13 92,159,413 (GRCm39) missense probably damaging 0.97
IGL03342:Rasgrf2 APN 13 92,136,098 (GRCm39) missense probably damaging 1.00
IGL03405:Rasgrf2 APN 13 92,044,170 (GRCm39) missense probably damaging 1.00
R0620:Rasgrf2 UTSW 13 92,067,936 (GRCm39) splice site probably benign
R0632:Rasgrf2 UTSW 13 92,120,393 (GRCm39) missense probably benign 0.00
R0894:Rasgrf2 UTSW 13 92,130,890 (GRCm39) missense probably damaging 1.00
R1354:Rasgrf2 UTSW 13 92,165,174 (GRCm39) missense probably damaging 1.00
R1400:Rasgrf2 UTSW 13 92,035,808 (GRCm39) missense probably damaging 1.00
R1437:Rasgrf2 UTSW 13 92,167,396 (GRCm39) missense probably damaging 1.00
R1443:Rasgrf2 UTSW 13 92,131,795 (GRCm39) missense probably damaging 1.00
R1522:Rasgrf2 UTSW 13 92,044,205 (GRCm39) missense probably benign 0.00
R1553:Rasgrf2 UTSW 13 92,038,783 (GRCm39) missense probably damaging 1.00
R1613:Rasgrf2 UTSW 13 92,050,740 (GRCm39) missense probably damaging 1.00
R1883:Rasgrf2 UTSW 13 92,117,149 (GRCm39) missense probably benign
R1934:Rasgrf2 UTSW 13 92,131,825 (GRCm39) splice site probably null
R1990:Rasgrf2 UTSW 13 92,172,473 (GRCm39) missense probably damaging 1.00
R2037:Rasgrf2 UTSW 13 92,050,748 (GRCm39) missense probably damaging 0.99
R2043:Rasgrf2 UTSW 13 92,167,351 (GRCm39) missense possibly damaging 0.91
R2135:Rasgrf2 UTSW 13 92,120,374 (GRCm39) missense probably benign
R2193:Rasgrf2 UTSW 13 92,160,221 (GRCm39) splice site probably null
R2406:Rasgrf2 UTSW 13 92,120,359 (GRCm39) missense probably benign
R3055:Rasgrf2 UTSW 13 92,165,583 (GRCm39) missense probably damaging 1.00
R3916:Rasgrf2 UTSW 13 92,167,296 (GRCm39) missense probably damaging 1.00
R3954:Rasgrf2 UTSW 13 92,130,974 (GRCm39) missense probably damaging 0.98
R3955:Rasgrf2 UTSW 13 92,130,974 (GRCm39) missense probably damaging 0.98
R3956:Rasgrf2 UTSW 13 92,130,974 (GRCm39) missense probably damaging 0.98
R4133:Rasgrf2 UTSW 13 92,130,773 (GRCm39) missense possibly damaging 0.59
R4177:Rasgrf2 UTSW 13 92,038,717 (GRCm39) missense probably damaging 1.00
R4178:Rasgrf2 UTSW 13 92,038,717 (GRCm39) missense probably damaging 1.00
R4357:Rasgrf2 UTSW 13 92,038,796 (GRCm39) missense probably damaging 1.00
R4358:Rasgrf2 UTSW 13 92,038,796 (GRCm39) missense probably damaging 1.00
R4359:Rasgrf2 UTSW 13 92,038,796 (GRCm39) missense probably damaging 1.00
R4439:Rasgrf2 UTSW 13 92,131,797 (GRCm39) missense possibly damaging 0.95
R4440:Rasgrf2 UTSW 13 92,131,797 (GRCm39) missense possibly damaging 0.95
R4441:Rasgrf2 UTSW 13 92,131,797 (GRCm39) missense possibly damaging 0.95
R4564:Rasgrf2 UTSW 13 92,033,773 (GRCm39) nonsense probably null
R4576:Rasgrf2 UTSW 13 92,044,529 (GRCm39) missense possibly damaging 0.58
R4590:Rasgrf2 UTSW 13 92,174,789 (GRCm39) missense probably damaging 1.00
R4718:Rasgrf2 UTSW 13 92,138,716 (GRCm39) critical splice donor site probably null
R4778:Rasgrf2 UTSW 13 92,131,780 (GRCm39) missense probably damaging 0.99
R4790:Rasgrf2 UTSW 13 92,136,135 (GRCm39) missense probably damaging 1.00
R4808:Rasgrf2 UTSW 13 92,160,190 (GRCm39) missense probably damaging 1.00
R5151:Rasgrf2 UTSW 13 92,044,155 (GRCm39) missense probably damaging 1.00
R5286:Rasgrf2 UTSW 13 92,267,941 (GRCm39) missense possibly damaging 0.94
R5902:Rasgrf2 UTSW 13 92,068,011 (GRCm39) missense probably damaging 1.00
R6180:Rasgrf2 UTSW 13 92,165,609 (GRCm39) missense probably damaging 1.00
R6264:Rasgrf2 UTSW 13 92,167,293 (GRCm39) missense probably damaging 1.00
R6369:Rasgrf2 UTSW 13 92,267,954 (GRCm39) missense probably benign
R6428:Rasgrf2 UTSW 13 92,136,100 (GRCm39) missense probably damaging 1.00
R6595:Rasgrf2 UTSW 13 92,167,361 (GRCm39) missense probably damaging 1.00
R6619:Rasgrf2 UTSW 13 92,165,027 (GRCm39) missense probably damaging 1.00
R6988:Rasgrf2 UTSW 13 92,033,754 (GRCm39) missense probably benign 0.02
R7026:Rasgrf2 UTSW 13 92,131,732 (GRCm39) missense probably damaging 1.00
R7038:Rasgrf2 UTSW 13 92,130,952 (GRCm39) missense possibly damaging 0.95
R7045:Rasgrf2 UTSW 13 92,159,100 (GRCm39) intron probably benign
R7056:Rasgrf2 UTSW 13 92,167,203 (GRCm39) missense probably damaging 0.99
R7058:Rasgrf2 UTSW 13 92,034,521 (GRCm39) missense probably damaging 0.99
R7256:Rasgrf2 UTSW 13 92,032,637 (GRCm39) nonsense probably null
R7392:Rasgrf2 UTSW 13 92,041,856 (GRCm39) missense
R7469:Rasgrf2 UTSW 13 92,165,530 (GRCm39) critical splice donor site probably null
R7618:Rasgrf2 UTSW 13 92,136,085 (GRCm39) missense
R7641:Rasgrf2 UTSW 13 92,267,914 (GRCm39) missense possibly damaging 0.65
R7674:Rasgrf2 UTSW 13 92,267,914 (GRCm39) missense possibly damaging 0.65
R7962:Rasgrf2 UTSW 13 92,167,300 (GRCm39) missense probably damaging 0.99
R8056:Rasgrf2 UTSW 13 92,167,321 (GRCm39) missense probably damaging 0.97
R8218:Rasgrf2 UTSW 13 92,130,796 (GRCm39) missense
R8796:Rasgrf2 UTSW 13 92,038,685 (GRCm39) missense
R8913:Rasgrf2 UTSW 13 92,159,034 (GRCm39) missense probably benign 0.05
R8971:Rasgrf2 UTSW 13 92,158,225 (GRCm39) missense possibly damaging 0.80
R9020:Rasgrf2 UTSW 13 92,165,146 (GRCm39) missense possibly damaging 0.93
R9487:Rasgrf2 UTSW 13 92,267,759 (GRCm39) missense probably benign
R9562:Rasgrf2 UTSW 13 92,034,469 (GRCm39) critical splice donor site probably null
R9712:Rasgrf2 UTSW 13 92,136,092 (GRCm39) missense
R9766:Rasgrf2 UTSW 13 92,160,188 (GRCm39) missense probably damaging 1.00
R9800:Rasgrf2 UTSW 13 92,267,860 (GRCm39) missense probably damaging 0.99
X0013:Rasgrf2 UTSW 13 92,167,363 (GRCm39) missense probably damaging 1.00
X0026:Rasgrf2 UTSW 13 92,050,654 (GRCm39) missense probably damaging 0.99
Z1177:Rasgrf2 UTSW 13 92,159,081 (GRCm39) missense unknown
Z1177:Rasgrf2 UTSW 13 92,131,632 (GRCm39) missense
Predicted Primers PCR Primer
(F):5'- GCTAAAACACCTTGAGATGGAGAC -3'
(R):5'- GTATGATTAGGTCCTCAGACGGC -3'

Sequencing Primer
(F):5'- TTGAGATGGAGACTCCCACC -3'
(R):5'- TCCTCAGACGGCACAGGAC -3'
Posted On 2019-11-26