Mutagenetix > Incidental Mutation

Incidental Mutation 'R7787:Or8g19'

599599

Institutional Source

Beutler Lab

Gene Symbol

Or8g19

Ensembl Gene

ENSMUSG00000049708

Gene Name

olfactory receptor family 8 subfamily G member 19

Synonyms

MOR171-6, GA_x6K02T2PVTD-32841223-32842158, Olfr27, MTPCR56, Olfr242, GA_x6K02T2KYVW-1037-120

MMRRC Submission

045843-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R7787 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

39039463-39056368 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 39055548 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 51 (L51F)

Ref Sequence

ENSEMBL: ENSMUSP00000151012 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000214052] [ENSMUST00000216405]

AlphaFold

Q9EQ90

Predicted Effect

probably benign
Transcript: ENSMUST00000214052
AA Change: L51F

PolyPhen 2 Score 0.225 (Sensitivity: 0.91; Specificity: 0.88)

Predicted Effect

probably benign
Transcript: ENSMUST00000216405
AA Change: L51F

PolyPhen 2 Score 0.225 (Sensitivity: 0.91; Specificity: 0.88)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	T	A	7: 27,263,926 (GRCm39)	I38N	probably damaging	Het
Abcb4	G	A	5: 8,959,220 (GRCm39)	V216M	probably damaging	Het
Abcc2	G	A	19: 43,772,685 (GRCm39)	V32M	probably damaging	Het
Adgrb3	C	A	1: 25,471,625 (GRCm39)	V714F	probably damaging	Het
Ahnak	T	A	19: 8,986,679 (GRCm39)	D2654E	unknown	Het
Ak8	T	A	2: 28,602,324 (GRCm39)	I86N	probably damaging	Het
Apob	G	A	12: 8,040,780 (GRCm39)	R635Q	probably damaging	Het
Atp10b	G	A	11: 43,150,700 (GRCm39)	R1466H	possibly damaging	Het
Bltp1	A	C	3: 36,939,557 (GRCm39)	H137P	probably damaging	Het
Btnl9	T	C	11: 49,066,866 (GRCm39)	T252A	unknown	Het
Cacna1e	T	A	1: 154,358,314 (GRCm39)	I648F	probably damaging	Het
Camkk1	A	G	11: 72,917,412 (GRCm39)	D121G	probably benign	Het
Cc2d1a	C	A	8: 84,860,144 (GRCm39)	Q904H	possibly damaging	Het
Cd1d1	A	G	3: 86,904,903 (GRCm39)	S212P	probably damaging	Het
Cfap69	C	T	5: 5,639,260 (GRCm39)	C638Y	probably damaging	Het
Cilk1	G	C	9: 78,074,902 (GRCm39)	V586L	probably benign	Het
Clca4a	A	G	3: 144,659,594 (GRCm39)	V754A	probably benign	Het
Cnpy4	T	A	5: 138,190,900 (GRCm39)	H187Q	probably benign	Het
Cpne5	A	T	17: 29,407,261 (GRCm39)		probably null	Het
Crnkl1	A	T	2: 145,767,515 (GRCm39)	N359K	probably benign	Het
Cyb561	A	T	11: 105,828,466 (GRCm39)	L63H	probably damaging	Het
Dock4	A	G	12: 40,775,676 (GRCm39)	T540A	probably benign	Het
Edc4	T	A	8: 106,614,146 (GRCm39)	Y7*	probably null	Het
Fstl5	A	C	3: 76,337,131 (GRCm39)	D230A	probably damaging	Het
Fthl17b	C	T	X: 8,829,043 (GRCm39)	R9Q	possibly damaging	Het
Fthl17b	C	T	X: 8,829,047 (GRCm39)	V8M	possibly damaging	Het
H2bw2	G	A	X: 135,828,471 (GRCm39)	R120K	unknown	Het
Hecw1	T	C	13: 14,493,494 (GRCm39)	Q337R	probably damaging	Het
Hmcn1	A	T	1: 150,632,343 (GRCm39)	Y865N	probably damaging	Het
Hyal6	A	G	6: 24,743,735 (GRCm39)	Y477C	probably damaging	Het
Ifi205	C	T	1: 173,842,640 (GRCm39)	G352E	probably damaging	Het
Ifi205	T	A	1: 173,842,644 (GRCm39)	S351C	probably damaging	Het
Irak3	C	A	10: 120,012,256 (GRCm39)	Q169H	probably benign	Het
Itgb1	T	A	8: 129,453,499 (GRCm39)	N99K	probably benign	Het
Kng2	A	G	16: 22,818,598 (GRCm39)	F298S	probably damaging	Het
Kri1	T	C	9: 21,192,380 (GRCm39)	E256G		Het
Lamc3	T	C	2: 31,790,551 (GRCm39)	I257T	probably damaging	Het
Mas1	A	C	17: 13,061,374 (GRCm39)	N16K	possibly damaging	Het
Mdn1	T	A	4: 32,741,794 (GRCm39)	L3855Q	probably damaging	Het
Muc4	C	G	16: 32,575,221 (GRCm39)	Q1269E	probably benign	Het
Mybph	G	A	1: 134,125,246 (GRCm39)	G258D	probably benign	Het
Mycbp2	A	C	14: 103,364,533 (GRCm39)	H4358Q	probably damaging	Het
Nat10	T	C	2: 103,552,208 (GRCm39)	D1012G	unknown	Het
Nub1	C	A	5: 24,913,801 (GRCm39)	Q561K	probably benign	Het
Nynrin	A	G	14: 56,107,980 (GRCm39)	N1029S	probably benign	Het
Or52x1	A	T	7: 104,853,252 (GRCm39)	C99*	probably null	Het
Or56a4	T	G	7: 104,806,401 (GRCm39)	I163L	probably benign	Het
Or5k15	A	G	16: 58,709,953 (GRCm39)	F210S	probably benign	Het
Or6e1	A	G	14: 54,520,169 (GRCm39)	L61P	probably damaging	Het
Pcsk1	T	A	13: 75,280,277 (GRCm39)	Y701N	possibly damaging	Het
Pglyrp4	C	G	3: 90,640,295 (GRCm39)	H182D	probably damaging	Het
Phaf1	T	G	8: 105,957,820 (GRCm39)	V42G	probably damaging	Het
Pkp4	C	A	2: 59,152,881 (GRCm39)	D576E	probably damaging	Het
Plec	A	G	15: 76,083,811 (GRCm39)	V17A	unknown	Het
Polq	T	A	16: 36,837,671 (GRCm39)	N194K	probably damaging	Het
Pou4f1	A	T	14: 104,703,460 (GRCm39)	M324K	unknown	Het
Ppa2	G	T	3: 133,036,259 (GRCm39)	G138W	probably damaging	Het
Prob1	A	T	18: 35,785,285 (GRCm39)	F990I	possibly damaging	Het
Proser1	T	A	3: 53,380,969 (GRCm39)	I182N	probably damaging	Het
Sgk3	T	C	1: 9,952,016 (GRCm39)	L214P	probably damaging	Het
Sipa1l1	T	C	12: 82,496,762 (GRCm39)	S1765P	possibly damaging	Het
Slc15a2	G	A	16: 36,572,228 (GRCm39)	S712L	probably benign	Het
Slc38a9	T	C	13: 112,825,880 (GRCm39)	L106P	probably damaging	Het
Slc43a2	G	A	11: 75,453,900 (GRCm39)	R271H	probably damaging	Het
Spsb4	T	A	9: 96,877,643 (GRCm39)	I227F	probably damaging	Het
Srgap3	C	T	6: 112,752,520 (GRCm39)	M321I	probably benign	Het
Stam2	T	C	2: 52,596,418 (GRCm39)	I340V	probably benign	Het
Tbc1d5	T	A	17: 51,181,711 (GRCm39)	R341*	probably null	Het
Terf2ip	A	C	8: 112,742,087 (GRCm39)	E260D	probably damaging	Het
Tert	G	T	13: 73,797,051 (GRCm39)	K1096N	probably damaging	Het
Ttc32	T	A	12: 9,088,083 (GRCm39)	D103E	probably benign	Het
Ube3d	T	A	9: 86,254,395 (GRCm39)	Q362L	possibly damaging	Het
Unc5c	G	A	3: 141,474,313 (GRCm39)	G295D	probably damaging	Het
Wnt7b	T	A	15: 85,428,112 (GRCm39)	I117F	probably damaging	Het
Zcchc7	T	C	4: 44,895,043 (GRCm39)		probably null	Het
Zfp619	A	G	7: 39,186,226 (GRCm39)	Q752R	possibly damaging	Het
Zkscan3	T	C	13: 21,572,034 (GRCm39)	K533E	possibly damaging	Het

Other mutations in Or8g19

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00226:Or8g19	APN	9	39,056,053 (GRCm39)	missense	possibly damaging	0.94
IGL00905:Or8g19	APN	9	39,056,326 (GRCm39)	missense	probably damaging	1.00
IGL02346:Or8g19	APN	9	39,055,939 (GRCm39)	missense	probably damaging	1.00
R0117:Or8g19	UTSW	9	39,056,146 (GRCm39)	missense	probably damaging	1.00
R0118:Or8g19	UTSW	9	39,055,399 (GRCm39)	start codon destroyed	probably null	0.97
R0590:Or8g19	UTSW	9	39,056,017 (GRCm39)	missense	probably benign	0.01
R2915:Or8g19	UTSW	9	39,055,762 (GRCm39)	missense	possibly damaging	0.94
R4299:Or8g19	UTSW	9	39,056,295 (GRCm39)	missense	probably benign	0.11
R4367:Or8g19	UTSW	9	39,055,725 (GRCm39)	missense	probably damaging	0.98
R4663:Or8g19	UTSW	9	39,056,145 (GRCm39)	missense	probably damaging	0.97
R5276:Or8g19	UTSW	9	39,055,611 (GRCm39)	missense	probably damaging	1.00
R5503:Or8g19	UTSW	9	39,055,780 (GRCm39)	missense	probably benign	0.02
R5742:Or8g19	UTSW	9	39,055,974 (GRCm39)	missense	probably benign	0.07
R5986:Or8g19	UTSW	9	39,056,278 (GRCm39)	missense	probably null	1.00
R6801:Or8g19	UTSW	9	39,055,506 (GRCm39)	missense	probably benign	0.01
R7247:Or8g19	UTSW	9	39,056,153 (GRCm39)	nonsense	probably null
R7520:Or8g19	UTSW	9	39,055,414 (GRCm39)	missense	probably benign
R8360:Or8g19	UTSW	9	39,055,761 (GRCm39)	nonsense	probably null
R8721:Or8g19	UTSW	9	39,055,386 (GRCm39)	start gained	probably benign
R8880:Or8g19	UTSW	9	39,055,899 (GRCm39)	missense	probably damaging	1.00
R8883:Or8g19	UTSW	9	39,056,083 (GRCm39)	missense	probably benign	0.00
R9143:Or8g19	UTSW	9	39,055,722 (GRCm39)	missense	possibly damaging	0.94
R9445:Or8g19	UTSW	9	39,055,766 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCTCCTTTAGATTTGATTGGAGGAC -3'
(R):5'- ATAGCAACATATCGGTCATATGCC -3'

Sequencing Primer
(F):5'- ATTTGATTGGAGGACATGGCAGC -3'
(R):5'- CAACATATCGGTCATATGCCATTGC -3'

Posted On

2019-11-26