Mutagenetix > Incidental Mutation

Incidental Mutation 'R7787:Btnl9'

599605

Institutional Source

Beutler Lab

Gene Symbol

Btnl9

Ensembl Gene

ENSMUSG00000040283

Gene Name

butyrophilin-like 9

Synonyms

D330012D11Rik

MMRRC Submission

045843-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R7787 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

49059152-49077916 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 49066866 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 252 (T252A)

Ref Sequence

ENSEMBL: ENSMUSP00000066598 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046522] [ENSMUST00000066531] [ENSMUST00000153999]

AlphaFold

Q8BJE2

Predicted Effect

probably benign
Transcript: ENSMUST00000046522

SMART Domains

Protein: ENSMUSP00000046229
Gene: ENSMUSG00000040283

Domain	Start	End	E-Value	Type
IG	44	151	1.24e-8	SMART
Pfam:Ig_2	155	243	9.2e-3	PFAM
Pfam:C2-set_2	156	238	1.7e-9	PFAM
transmembrane domain	259	281	N/A	INTRINSIC
PRY	324	377	8.68e-14	SMART
SPRY	378	503	1.3e-18	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000066531
AA Change: T252A

SMART Domains

Protein: ENSMUSP00000066598
Gene: ENSMUSG00000040283
AA Change: T252A

Domain	Start	End	E-Value	Type
IG	44	151	1.24e-8	SMART
Pfam:Ig_3	155	231	1e-4	PFAM
Pfam:C2-set_2	156	238	2.2e-6	PFAM
transmembrane domain	360	382	N/A	INTRINSIC
PRY	419	462	3.61e-2	SMART
SPRY	463	588	1.3e-18	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000153999

SMART Domains

Protein: ENSMUSP00000120530
Gene: ENSMUSG00000040283

Domain	Start	End	E-Value	Type
IG	44	151	1.24e-8	SMART
transmembrane domain	165	187	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (74/74)

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	T	A	7: 27,263,926 (GRCm39)	I38N	probably damaging	Het
Abcb4	G	A	5: 8,959,220 (GRCm39)	V216M	probably damaging	Het
Abcc2	G	A	19: 43,772,685 (GRCm39)	V32M	probably damaging	Het
Adgrb3	C	A	1: 25,471,625 (GRCm39)	V714F	probably damaging	Het
Ahnak	T	A	19: 8,986,679 (GRCm39)	D2654E	unknown	Het
Ak8	T	A	2: 28,602,324 (GRCm39)	I86N	probably damaging	Het
Apob	G	A	12: 8,040,780 (GRCm39)	R635Q	probably damaging	Het
Atp10b	G	A	11: 43,150,700 (GRCm39)	R1466H	possibly damaging	Het
Bltp1	A	C	3: 36,939,557 (GRCm39)	H137P	probably damaging	Het
Cacna1e	T	A	1: 154,358,314 (GRCm39)	I648F	probably damaging	Het
Camkk1	A	G	11: 72,917,412 (GRCm39)	D121G	probably benign	Het
Cc2d1a	C	A	8: 84,860,144 (GRCm39)	Q904H	possibly damaging	Het
Cd1d1	A	G	3: 86,904,903 (GRCm39)	S212P	probably damaging	Het
Cfap69	C	T	5: 5,639,260 (GRCm39)	C638Y	probably damaging	Het
Cilk1	G	C	9: 78,074,902 (GRCm39)	V586L	probably benign	Het
Clca4a	A	G	3: 144,659,594 (GRCm39)	V754A	probably benign	Het
Cnpy4	T	A	5: 138,190,900 (GRCm39)	H187Q	probably benign	Het
Cpne5	A	T	17: 29,407,261 (GRCm39)		probably null	Het
Crnkl1	A	T	2: 145,767,515 (GRCm39)	N359K	probably benign	Het
Cyb561	A	T	11: 105,828,466 (GRCm39)	L63H	probably damaging	Het
Dock4	A	G	12: 40,775,676 (GRCm39)	T540A	probably benign	Het
Edc4	T	A	8: 106,614,146 (GRCm39)	Y7*	probably null	Het
Fstl5	A	C	3: 76,337,131 (GRCm39)	D230A	probably damaging	Het
Fthl17b	C	T	X: 8,829,043 (GRCm39)	R9Q	possibly damaging	Het
Fthl17b	C	T	X: 8,829,047 (GRCm39)	V8M	possibly damaging	Het
H2bw2	G	A	X: 135,828,471 (GRCm39)	R120K	unknown	Het
Hecw1	T	C	13: 14,493,494 (GRCm39)	Q337R	probably damaging	Het
Hmcn1	A	T	1: 150,632,343 (GRCm39)	Y865N	probably damaging	Het
Hyal6	A	G	6: 24,743,735 (GRCm39)	Y477C	probably damaging	Het
Ifi205	C	T	1: 173,842,640 (GRCm39)	G352E	probably damaging	Het
Ifi205	T	A	1: 173,842,644 (GRCm39)	S351C	probably damaging	Het
Irak3	C	A	10: 120,012,256 (GRCm39)	Q169H	probably benign	Het
Itgb1	T	A	8: 129,453,499 (GRCm39)	N99K	probably benign	Het
Kng2	A	G	16: 22,818,598 (GRCm39)	F298S	probably damaging	Het
Kri1	T	C	9: 21,192,380 (GRCm39)	E256G		Het
Lamc3	T	C	2: 31,790,551 (GRCm39)	I257T	probably damaging	Het
Mas1	A	C	17: 13,061,374 (GRCm39)	N16K	possibly damaging	Het
Mdn1	T	A	4: 32,741,794 (GRCm39)	L3855Q	probably damaging	Het
Muc4	C	G	16: 32,575,221 (GRCm39)	Q1269E	probably benign	Het
Mybph	G	A	1: 134,125,246 (GRCm39)	G258D	probably benign	Het
Mycbp2	A	C	14: 103,364,533 (GRCm39)	H4358Q	probably damaging	Het
Nat10	T	C	2: 103,552,208 (GRCm39)	D1012G	unknown	Het
Nub1	C	A	5: 24,913,801 (GRCm39)	Q561K	probably benign	Het
Nynrin	A	G	14: 56,107,980 (GRCm39)	N1029S	probably benign	Het
Or52x1	A	T	7: 104,853,252 (GRCm39)	C99*	probably null	Het
Or56a4	T	G	7: 104,806,401 (GRCm39)	I163L	probably benign	Het
Or5k15	A	G	16: 58,709,953 (GRCm39)	F210S	probably benign	Het
Or6e1	A	G	14: 54,520,169 (GRCm39)	L61P	probably damaging	Het
Or8g19	C	T	9: 39,055,548 (GRCm39)	L51F	probably benign	Het
Pcsk1	T	A	13: 75,280,277 (GRCm39)	Y701N	possibly damaging	Het
Pglyrp4	C	G	3: 90,640,295 (GRCm39)	H182D	probably damaging	Het
Phaf1	T	G	8: 105,957,820 (GRCm39)	V42G	probably damaging	Het
Pkp4	C	A	2: 59,152,881 (GRCm39)	D576E	probably damaging	Het
Plec	A	G	15: 76,083,811 (GRCm39)	V17A	unknown	Het
Polq	T	A	16: 36,837,671 (GRCm39)	N194K	probably damaging	Het
Pou4f1	A	T	14: 104,703,460 (GRCm39)	M324K	unknown	Het
Ppa2	G	T	3: 133,036,259 (GRCm39)	G138W	probably damaging	Het
Prob1	A	T	18: 35,785,285 (GRCm39)	F990I	possibly damaging	Het
Proser1	T	A	3: 53,380,969 (GRCm39)	I182N	probably damaging	Het
Sgk3	T	C	1: 9,952,016 (GRCm39)	L214P	probably damaging	Het
Sipa1l1	T	C	12: 82,496,762 (GRCm39)	S1765P	possibly damaging	Het
Slc15a2	G	A	16: 36,572,228 (GRCm39)	S712L	probably benign	Het
Slc38a9	T	C	13: 112,825,880 (GRCm39)	L106P	probably damaging	Het
Slc43a2	G	A	11: 75,453,900 (GRCm39)	R271H	probably damaging	Het
Spsb4	T	A	9: 96,877,643 (GRCm39)	I227F	probably damaging	Het
Srgap3	C	T	6: 112,752,520 (GRCm39)	M321I	probably benign	Het
Stam2	T	C	2: 52,596,418 (GRCm39)	I340V	probably benign	Het
Tbc1d5	T	A	17: 51,181,711 (GRCm39)	R341*	probably null	Het
Terf2ip	A	C	8: 112,742,087 (GRCm39)	E260D	probably damaging	Het
Tert	G	T	13: 73,797,051 (GRCm39)	K1096N	probably damaging	Het
Ttc32	T	A	12: 9,088,083 (GRCm39)	D103E	probably benign	Het
Ube3d	T	A	9: 86,254,395 (GRCm39)	Q362L	possibly damaging	Het
Unc5c	G	A	3: 141,474,313 (GRCm39)	G295D	probably damaging	Het
Wnt7b	T	A	15: 85,428,112 (GRCm39)	I117F	probably damaging	Het
Zcchc7	T	C	4: 44,895,043 (GRCm39)		probably null	Het
Zfp619	A	G	7: 39,186,226 (GRCm39)	Q752R	possibly damaging	Het
Zkscan3	T	C	13: 21,572,034 (GRCm39)	K533E	possibly damaging	Het

Other mutations in Btnl9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01155:Btnl9	APN	11	49,066,518 (GRCm39)	missense	probably damaging	1.00
IGL01923:Btnl9	APN	11	49,071,409 (GRCm39)	missense	probably benign	0.00
IGL02129:Btnl9	APN	11	49,060,100 (GRCm39)	missense	probably damaging	1.00
IGL02248:Btnl9	APN	11	49,071,625 (GRCm39)	missense	probably benign	0.27
IGL02795:Btnl9	APN	11	49,065,694 (GRCm39)	splice site	probably benign
IGL02889:Btnl9	APN	11	49,069,604 (GRCm39)	missense	probably damaging	1.00
IGL02796:Btnl9	UTSW	11	49,060,008 (GRCm39)	missense	probably damaging	0.99
R0084:Btnl9	UTSW	11	49,069,606 (GRCm39)	missense	possibly damaging	0.91
R0362:Btnl9	UTSW	11	49,060,443 (GRCm39)	missense	possibly damaging	0.73
R0417:Btnl9	UTSW	11	49,066,422 (GRCm39)	missense	probably damaging	1.00
R1199:Btnl9	UTSW	11	49,071,574 (GRCm39)	missense	probably damaging	1.00
R1260:Btnl9	UTSW	11	49,060,371 (GRCm39)	missense	probably damaging	0.98
R1802:Btnl9	UTSW	11	49,066,617 (GRCm39)	missense	probably benign	0.06
R2000:Btnl9	UTSW	11	49,059,948 (GRCm39)	missense	probably benign	0.04
R2068:Btnl9	UTSW	11	49,060,390 (GRCm39)	missense	probably damaging	0.98
R2130:Btnl9	UTSW	11	49,071,523 (GRCm39)	missense	probably damaging	0.99
R2142:Btnl9	UTSW	11	49,061,453 (GRCm39)	splice site	probably null
R2229:Btnl9	UTSW	11	49,059,945 (GRCm39)	missense	probably damaging	1.00
R2255:Btnl9	UTSW	11	49,060,143 (GRCm39)	nonsense	probably null
R2386:Btnl9	UTSW	11	49,069,602 (GRCm39)	missense	probably damaging	1.00
R3177:Btnl9	UTSW	11	49,060,503 (GRCm39)	missense	probably damaging	1.00
R3277:Btnl9	UTSW	11	49,060,503 (GRCm39)	missense	probably damaging	1.00
R3835:Btnl9	UTSW	11	49,071,512 (GRCm39)	missense	probably damaging	1.00
R5287:Btnl9	UTSW	11	49,060,434 (GRCm39)	missense	probably benign	0.20
R5352:Btnl9	UTSW	11	49,069,667 (GRCm39)	missense	probably benign	0.01
R5433:Btnl9	UTSW	11	49,066,830 (GRCm39)	intron	probably benign
R5490:Btnl9	UTSW	11	49,060,395 (GRCm39)	missense	probably damaging	1.00
R5576:Btnl9	UTSW	11	49,069,712 (GRCm39)	missense	probably benign	0.00
R6008:Btnl9	UTSW	11	49,073,792 (GRCm39)	critical splice donor site	probably null
R6770:Btnl9	UTSW	11	49,066,392 (GRCm39)	splice site	probably null
R7126:Btnl9	UTSW	11	49,060,082 (GRCm39)	missense	probably damaging	1.00
R7276:Btnl9	UTSW	11	49,066,617 (GRCm39)	missense	probably benign	0.06
R7923:Btnl9	UTSW	11	49,071,565 (GRCm39)	missense	probably damaging	0.97
R8050:Btnl9	UTSW	11	49,066,442 (GRCm39)	missense	probably benign	0.25
R8558:Btnl9	UTSW	11	49,071,619 (GRCm39)	missense	probably benign	0.00
R8788:Btnl9	UTSW	11	49,066,614 (GRCm39)	missense	probably benign	0.03
R8945:Btnl9	UTSW	11	49,065,661 (GRCm39)	missense	probably benign	0.38
R9105:Btnl9	UTSW	11	49,066,461 (GRCm39)	missense	probably benign	0.29
R9656:Btnl9	UTSW	11	49,060,008 (GRCm39)	missense	probably damaging	0.99
X0026:Btnl9	UTSW	11	49,060,068 (GRCm39)	missense	probably damaging	1.00
Z1192:Btnl9	UTSW	11	49,066,805 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCTTCAGTGGGAGCCTCTCTAG -3'
(R):5'- CTGTTATTACCCCAGAGCCC -3'

Sequencing Primer
(F):5'- GAGCCTCTCTAGCCAGGTATGAAC -3'
(R):5'- ATTACCCCAGAGCCCATTTTC -3'

Posted On

2019-11-26