Mutagenetix > Incidental Mutation

Incidental Mutation 'R7787:Apob'

599609

Institutional Source

Beutler Lab

Gene Symbol

Apob

Ensembl Gene

ENSMUSG00000020609

Gene Name

apolipoprotein B

Synonyms

apob-100, apob-48

MMRRC Submission

045843-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.859) question?

Stock #

R7787 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

8027648-8066835 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 8040780 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 635 (R635Q)

Ref Sequence

ENSEMBL: ENSMUSP00000036044 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037520] [ENSMUST00000037811] [ENSMUST00000171271]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000037520
AA Change: R622Q

PolyPhen 2 Score 0.392 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000035761
Gene: ENSMUSG00000020609
AA Change: R622Q

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	33	585	6.03e-94	SMART
DUF1943	619	932	7.88e-97	SMART
Pfam:DUF1081	945	1059	9.4e-32	PFAM
low complexity region	1100	1109	N/A	INTRINSIC
Blast:LPD_N	1249	1311	9e-22	BLAST
low complexity region	1632	1644	N/A	INTRINSIC
internal_repeat_1	1882	2038	6.61e-9	PROSPERO
SCOP:d1gw5a_	2105	2577	9e-5	SMART
internal_repeat_1	2973	3150	6.61e-9	PROSPERO
low complexity region	3561	3580	N/A	INTRINSIC
low complexity region	3928	3936	N/A	INTRINSIC
Pfam:ApoB100_C	4401	4456	5.6e-34	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000037811
AA Change: R635Q

PolyPhen 2 Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000036044
Gene: ENSMUSG00000020609
AA Change: R635Q

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	46	598	6.03e-94	SMART
DUF1943	632	945	7.88e-97	SMART
Pfam:DUF1081	960	1070	6.3e-39	PFAM
low complexity region	1113	1122	N/A	INTRINSIC
Blast:LPD_N	1282	1344	1e-21	BLAST
low complexity region	1665	1677	N/A	INTRINSIC
internal_repeat_1	1915	2071	6.6e-9	PROSPERO
SCOP:d1gw5a_	2138	2610	9e-5	SMART
internal_repeat_1	3006	3183	6.6e-9	PROSPERO
low complexity region	3594	3613	N/A	INTRINSIC
low complexity region	3961	3969	N/A	INTRINSIC
Pfam:ApoB100_C	4434	4490	1.6e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171271

SMART Domains

Protein: ENSMUSP00000127147
Gene: ENSMUSG00000020609

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
Pfam:Vitellogenin_N	45	324	6.7e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mutants usually die by midgestation and longer survivors exhibit exencephaly. Heterozygotes show reduced plasma cholesterol and apolipoprotein levels. Single isoform B100 and B48 null mutants are viable. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	T	A	7: 27,263,926 (GRCm39)	I38N	probably damaging	Het
Abcb4	G	A	5: 8,959,220 (GRCm39)	V216M	probably damaging	Het
Abcc2	G	A	19: 43,772,685 (GRCm39)	V32M	probably damaging	Het
Adgrb3	C	A	1: 25,471,625 (GRCm39)	V714F	probably damaging	Het
Ahnak	T	A	19: 8,986,679 (GRCm39)	D2654E	unknown	Het
Ak8	T	A	2: 28,602,324 (GRCm39)	I86N	probably damaging	Het
Atp10b	G	A	11: 43,150,700 (GRCm39)	R1466H	possibly damaging	Het
Bltp1	A	C	3: 36,939,557 (GRCm39)	H137P	probably damaging	Het
Btnl9	T	C	11: 49,066,866 (GRCm39)	T252A	unknown	Het
Cacna1e	T	A	1: 154,358,314 (GRCm39)	I648F	probably damaging	Het
Camkk1	A	G	11: 72,917,412 (GRCm39)	D121G	probably benign	Het
Cc2d1a	C	A	8: 84,860,144 (GRCm39)	Q904H	possibly damaging	Het
Cd1d1	A	G	3: 86,904,903 (GRCm39)	S212P	probably damaging	Het
Cfap69	C	T	5: 5,639,260 (GRCm39)	C638Y	probably damaging	Het
Cilk1	G	C	9: 78,074,902 (GRCm39)	V586L	probably benign	Het
Clca4a	A	G	3: 144,659,594 (GRCm39)	V754A	probably benign	Het
Cnpy4	T	A	5: 138,190,900 (GRCm39)	H187Q	probably benign	Het
Cpne5	A	T	17: 29,407,261 (GRCm39)		probably null	Het
Crnkl1	A	T	2: 145,767,515 (GRCm39)	N359K	probably benign	Het
Cyb561	A	T	11: 105,828,466 (GRCm39)	L63H	probably damaging	Het
Dock4	A	G	12: 40,775,676 (GRCm39)	T540A	probably benign	Het
Edc4	T	A	8: 106,614,146 (GRCm39)	Y7*	probably null	Het
Fstl5	A	C	3: 76,337,131 (GRCm39)	D230A	probably damaging	Het
Fthl17b	C	T	X: 8,829,043 (GRCm39)	R9Q	possibly damaging	Het
Fthl17b	C	T	X: 8,829,047 (GRCm39)	V8M	possibly damaging	Het
H2bw2	G	A	X: 135,828,471 (GRCm39)	R120K	unknown	Het
Hecw1	T	C	13: 14,493,494 (GRCm39)	Q337R	probably damaging	Het
Hmcn1	A	T	1: 150,632,343 (GRCm39)	Y865N	probably damaging	Het
Hyal6	A	G	6: 24,743,735 (GRCm39)	Y477C	probably damaging	Het
Ifi205	C	T	1: 173,842,640 (GRCm39)	G352E	probably damaging	Het
Ifi205	T	A	1: 173,842,644 (GRCm39)	S351C	probably damaging	Het
Irak3	C	A	10: 120,012,256 (GRCm39)	Q169H	probably benign	Het
Itgb1	T	A	8: 129,453,499 (GRCm39)	N99K	probably benign	Het
Kng2	A	G	16: 22,818,598 (GRCm39)	F298S	probably damaging	Het
Kri1	T	C	9: 21,192,380 (GRCm39)	E256G		Het
Lamc3	T	C	2: 31,790,551 (GRCm39)	I257T	probably damaging	Het
Mas1	A	C	17: 13,061,374 (GRCm39)	N16K	possibly damaging	Het
Mdn1	T	A	4: 32,741,794 (GRCm39)	L3855Q	probably damaging	Het
Muc4	C	G	16: 32,575,221 (GRCm39)	Q1269E	probably benign	Het
Mybph	G	A	1: 134,125,246 (GRCm39)	G258D	probably benign	Het
Mycbp2	A	C	14: 103,364,533 (GRCm39)	H4358Q	probably damaging	Het
Nat10	T	C	2: 103,552,208 (GRCm39)	D1012G	unknown	Het
Nub1	C	A	5: 24,913,801 (GRCm39)	Q561K	probably benign	Het
Nynrin	A	G	14: 56,107,980 (GRCm39)	N1029S	probably benign	Het
Or52x1	A	T	7: 104,853,252 (GRCm39)	C99*	probably null	Het
Or56a4	T	G	7: 104,806,401 (GRCm39)	I163L	probably benign	Het
Or5k15	A	G	16: 58,709,953 (GRCm39)	F210S	probably benign	Het
Or6e1	A	G	14: 54,520,169 (GRCm39)	L61P	probably damaging	Het
Or8g19	C	T	9: 39,055,548 (GRCm39)	L51F	probably benign	Het
Pcsk1	T	A	13: 75,280,277 (GRCm39)	Y701N	possibly damaging	Het
Pglyrp4	C	G	3: 90,640,295 (GRCm39)	H182D	probably damaging	Het
Phaf1	T	G	8: 105,957,820 (GRCm39)	V42G	probably damaging	Het
Pkp4	C	A	2: 59,152,881 (GRCm39)	D576E	probably damaging	Het
Plec	A	G	15: 76,083,811 (GRCm39)	V17A	unknown	Het
Polq	T	A	16: 36,837,671 (GRCm39)	N194K	probably damaging	Het
Pou4f1	A	T	14: 104,703,460 (GRCm39)	M324K	unknown	Het
Ppa2	G	T	3: 133,036,259 (GRCm39)	G138W	probably damaging	Het
Prob1	A	T	18: 35,785,285 (GRCm39)	F990I	possibly damaging	Het
Proser1	T	A	3: 53,380,969 (GRCm39)	I182N	probably damaging	Het
Sgk3	T	C	1: 9,952,016 (GRCm39)	L214P	probably damaging	Het
Sipa1l1	T	C	12: 82,496,762 (GRCm39)	S1765P	possibly damaging	Het
Slc15a2	G	A	16: 36,572,228 (GRCm39)	S712L	probably benign	Het
Slc38a9	T	C	13: 112,825,880 (GRCm39)	L106P	probably damaging	Het
Slc43a2	G	A	11: 75,453,900 (GRCm39)	R271H	probably damaging	Het
Spsb4	T	A	9: 96,877,643 (GRCm39)	I227F	probably damaging	Het
Srgap3	C	T	6: 112,752,520 (GRCm39)	M321I	probably benign	Het
Stam2	T	C	2: 52,596,418 (GRCm39)	I340V	probably benign	Het
Tbc1d5	T	A	17: 51,181,711 (GRCm39)	R341*	probably null	Het
Terf2ip	A	C	8: 112,742,087 (GRCm39)	E260D	probably damaging	Het
Tert	G	T	13: 73,797,051 (GRCm39)	K1096N	probably damaging	Het
Ttc32	T	A	12: 9,088,083 (GRCm39)	D103E	probably benign	Het
Ube3d	T	A	9: 86,254,395 (GRCm39)	Q362L	possibly damaging	Het
Unc5c	G	A	3: 141,474,313 (GRCm39)	G295D	probably damaging	Het
Wnt7b	T	A	15: 85,428,112 (GRCm39)	I117F	probably damaging	Het
Zcchc7	T	C	4: 44,895,043 (GRCm39)		probably null	Het
Zfp619	A	G	7: 39,186,226 (GRCm39)	Q752R	possibly damaging	Het
Zkscan3	T	C	13: 21,572,034 (GRCm39)	K533E	possibly damaging	Het

Other mutations in Apob

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Apob	APN	12	8,043,065 (GRCm39)	splice site	probably benign
IGL00421:Apob	APN	12	8,060,197 (GRCm39)	missense	probably damaging	0.99
IGL00658:Apob	APN	12	8,059,471 (GRCm39)	missense	probably benign	0.08
IGL00768:Apob	APN	12	8,052,107 (GRCm39)	missense	probably damaging	1.00
IGL00833:Apob	APN	12	8,060,101 (GRCm39)	missense	probably benign	0.14
IGL00926:Apob	APN	12	8,065,421 (GRCm39)	missense	probably benign	0.01
IGL01065:Apob	APN	12	8,053,299 (GRCm39)	missense	probably damaging	0.99
IGL01313:Apob	APN	12	8,050,898 (GRCm39)	missense	probably damaging	1.00
IGL01419:Apob	APN	12	8,052,251 (GRCm39)	missense	probably damaging	0.99
IGL01461:Apob	APN	12	8,051,884 (GRCm39)	missense	probably benign	0.13
IGL02002:Apob	APN	12	8,044,822 (GRCm39)	missense	probably benign	0.03
IGL02031:Apob	APN	12	8,065,222 (GRCm39)	missense	probably benign
IGL02102:Apob	APN	12	8,039,407 (GRCm39)	missense	possibly damaging	0.94
IGL02115:Apob	APN	12	8,042,923 (GRCm39)	missense	probably benign	0.06
IGL02513:Apob	APN	12	8,042,979 (GRCm39)	missense	probably benign	0.01
IGL02967:Apob	APN	12	8,065,366 (GRCm39)	nonsense	probably null
IGL03005:Apob	APN	12	8,043,059 (GRCm39)	splice site	probably benign
IGL03011:Apob	APN	12	8,047,883 (GRCm39)	missense	probably damaging	1.00
IGL03116:Apob	APN	12	8,066,350 (GRCm39)	missense	probably damaging	0.98
IGL03215:Apob	APN	12	8,063,818 (GRCm39)	missense	possibly damaging	0.92
IGL03227:Apob	APN	12	8,066,089 (GRCm39)	missense	probably benign	0.04
Aesthete	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
Essence	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
Ethos	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
IGL02835:Apob	UTSW	12	8,065,097 (GRCm39)	missense	possibly damaging	0.86
IGL02837:Apob	UTSW	12	8,055,102 (GRCm39)	missense	probably damaging	1.00
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0116:Apob	UTSW	12	8,039,113 (GRCm39)	unclassified	probably benign
R0180:Apob	UTSW	12	8,058,285 (GRCm39)	nonsense	probably null
R0288:Apob	UTSW	12	8,040,779 (GRCm39)	nonsense	probably null
R0295:Apob	UTSW	12	8,052,181 (GRCm39)	nonsense	probably null
R0305:Apob	UTSW	12	8,062,210 (GRCm39)	missense	probably damaging	1.00
R0312:Apob	UTSW	12	8,059,034 (GRCm39)	missense	probably benign
R0324:Apob	UTSW	12	8,060,521 (GRCm39)	missense	probably benign	0.41
R0326:Apob	UTSW	12	8,040,307 (GRCm39)	missense	probably damaging	1.00
R0363:Apob	UTSW	12	8,060,136 (GRCm39)	missense	probably damaging	1.00
R0390:Apob	UTSW	12	8,038,678 (GRCm39)	missense	probably damaging	0.99
R0462:Apob	UTSW	12	8,050,896 (GRCm39)	missense	probably damaging	1.00
R0471:Apob	UTSW	12	8,040,406 (GRCm39)	missense	probably damaging	1.00
R0532:Apob	UTSW	12	8,066,188 (GRCm39)	missense	possibly damaging	0.48
R0548:Apob	UTSW	12	8,056,282 (GRCm39)	missense	probably damaging	1.00
R0560:Apob	UTSW	12	8,055,101 (GRCm39)	missense	probably damaging	1.00
R0595:Apob	UTSW	12	8,058,369 (GRCm39)	missense	probably benign	0.01
R0600:Apob	UTSW	12	8,056,440 (GRCm39)	missense	probably damaging	1.00
R0626:Apob	UTSW	12	8,066,193 (GRCm39)	missense	probably benign	0.45
R0685:Apob	UTSW	12	8,060,742 (GRCm39)	missense	probably benign
R0765:Apob	UTSW	12	8,066,518 (GRCm39)	missense	probably benign
R0790:Apob	UTSW	12	8,060,245 (GRCm39)	missense	probably damaging	1.00
R0918:Apob	UTSW	12	8,033,941 (GRCm39)	missense	probably benign	0.10
R0962:Apob	UTSW	12	8,039,191 (GRCm39)	missense	probably damaging	0.98
R1055:Apob	UTSW	12	8,044,963 (GRCm39)	missense	probably damaging	1.00
R1077:Apob	UTSW	12	8,056,017 (GRCm39)	missense	probably benign
R1143:Apob	UTSW	12	8,062,354 (GRCm39)	missense	probably benign	0.26
R1163:Apob	UTSW	12	8,061,654 (GRCm39)	missense	probably damaging	1.00
R1266:Apob	UTSW	12	8,056,093 (GRCm39)	missense	probably benign	0.37
R1434:Apob	UTSW	12	8,059,715 (GRCm39)	missense	probably damaging	1.00
R1442:Apob	UTSW	12	8,036,165 (GRCm39)	missense	probably benign	0.31
R1445:Apob	UTSW	12	8,066,084 (GRCm39)	missense	possibly damaging	0.48
R1459:Apob	UTSW	12	8,061,937 (GRCm39)	missense	possibly damaging	0.92
R1459:Apob	UTSW	12	8,056,047 (GRCm39)	missense	probably benign
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1508:Apob	UTSW	12	8,061,481 (GRCm39)	missense	possibly damaging	0.92
R1518:Apob	UTSW	12	8,039,207 (GRCm39)	missense	probably benign	0.01
R1531:Apob	UTSW	12	8,047,880 (GRCm39)	missense	possibly damaging	0.65
R1547:Apob	UTSW	12	8,053,368 (GRCm39)	missense	probably benign	0.08
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1682:Apob	UTSW	12	8,062,365 (GRCm39)	missense	probably benign	0.00
R1709:Apob	UTSW	12	8,059,306 (GRCm39)	missense	probably damaging	0.98
R1718:Apob	UTSW	12	8,066,087 (GRCm39)	missense	probably benign	0.02
R1752:Apob	UTSW	12	8,038,766 (GRCm39)	missense	probably benign	0.01
R1781:Apob	UTSW	12	8,059,603 (GRCm39)	missense	possibly damaging	0.96
R1818:Apob	UTSW	12	8,063,064 (GRCm39)	missense	possibly damaging	0.93
R1818:Apob	UTSW	12	8,056,834 (GRCm39)	missense	probably damaging	0.98
R1842:Apob	UTSW	12	8,061,559 (GRCm39)	missense	probably damaging	1.00
R1843:Apob	UTSW	12	8,057,602 (GRCm39)	missense	possibly damaging	0.65
R1853:Apob	UTSW	12	8,060,928 (GRCm39)	nonsense	probably null
R1990:Apob	UTSW	12	8,051,039 (GRCm39)	missense	probably damaging	1.00
R2016:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2017:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2023:Apob	UTSW	12	8,061,090 (GRCm39)	missense	probably benign	0.01
R2037:Apob	UTSW	12	8,057,488 (GRCm39)	missense	probably benign	0.37
R2054:Apob	UTSW	12	8,063,134 (GRCm39)	missense	probably damaging	1.00
R2057:Apob	UTSW	12	8,052,164 (GRCm39)	nonsense	probably null
R2085:Apob	UTSW	12	8,062,240 (GRCm39)	missense	probably damaging	1.00
R2159:Apob	UTSW	12	8,060,081 (GRCm39)	missense	probably benign	0.12
R2209:Apob	UTSW	12	8,057,752 (GRCm39)	missense	probably benign	0.28
R2249:Apob	UTSW	12	8,057,499 (GRCm39)	missense	probably damaging	1.00
R2254:Apob	UTSW	12	8,061,256 (GRCm39)	missense	possibly damaging	0.92
R2265:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2266:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2267:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2268:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2296:Apob	UTSW	12	8,044,879 (GRCm39)	missense	probably damaging	0.97
R2897:Apob	UTSW	12	8,060,356 (GRCm39)	missense	probably damaging	1.00
R3431:Apob	UTSW	12	8,060,778 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,056,327 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,061,763 (GRCm39)	missense	possibly damaging	0.46
R3899:Apob	UTSW	12	8,065,849 (GRCm39)	missense	possibly damaging	0.87
R4020:Apob	UTSW	12	8,044,914 (GRCm39)	nonsense	probably null
R4050:Apob	UTSW	12	8,065,390 (GRCm39)	missense	probably benign	0.02
R4351:Apob	UTSW	12	8,043,054 (GRCm39)	missense	probably benign	0.03
R4365:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4366:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4456:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4458:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4600:Apob	UTSW	12	8,058,568 (GRCm39)	missense	probably damaging	1.00
R4611:Apob	UTSW	12	8,061,331 (GRCm39)	missense	probably damaging	1.00
R4646:Apob	UTSW	12	8,062,759 (GRCm39)	missense	probably benign	0.21
R4678:Apob	UTSW	12	8,045,585 (GRCm39)	missense	probably damaging	1.00
R4685:Apob	UTSW	12	8,056,456 (GRCm39)	missense	probably benign	0.00
R4707:Apob	UTSW	12	8,056,205 (GRCm39)	missense	probably damaging	0.96
R4726:Apob	UTSW	12	8,040,267 (GRCm39)	missense	probably damaging	0.98
R4792:Apob	UTSW	12	8,058,051 (GRCm39)	missense	probably benign	0.26
R4822:Apob	UTSW	12	8,065,741 (GRCm39)	missense	probably benign	0.04
R4834:Apob	UTSW	12	8,064,101 (GRCm39)	missense	possibly damaging	0.49
R4835:Apob	UTSW	12	8,065,391 (GRCm39)	missense	possibly damaging	0.56
R4887:Apob	UTSW	12	8,063,099 (GRCm39)	missense	probably damaging	1.00
R4910:Apob	UTSW	12	8,057,848 (GRCm39)	missense	probably damaging	1.00
R5072:Apob	UTSW	12	8,058,714 (GRCm39)	missense	probably benign	0.00
R5073:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5074:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5101:Apob	UTSW	12	8,061,934 (GRCm39)	missense	probably benign	0.09
R5123:Apob	UTSW	12	8,057,630 (GRCm39)	splice site	probably null
R5133:Apob	UTSW	12	8,058,898 (GRCm39)	missense	probably damaging	0.99
R5135:Apob	UTSW	12	8,060,086 (GRCm39)	missense	probably damaging	1.00
R5137:Apob	UTSW	12	8,061,384 (GRCm39)	missense	possibly damaging	0.63
R5160:Apob	UTSW	12	8,062,126 (GRCm39)	missense	possibly damaging	0.90
R5173:Apob	UTSW	12	8,058,238 (GRCm39)	missense	probably benign	0.00
R5202:Apob	UTSW	12	8,063,737 (GRCm39)	missense	probably damaging	0.98
R5229:Apob	UTSW	12	8,027,806 (GRCm39)	missense	probably benign
R5292:Apob	UTSW	12	8,055,912 (GRCm39)	missense	probably benign	0.01
R5378:Apob	UTSW	12	8,061,865 (GRCm39)	missense	probably damaging	0.99
R5494:Apob	UTSW	12	8,061,762 (GRCm39)	missense	probably damaging	0.99
R5517:Apob	UTSW	12	8,040,906 (GRCm39)	missense	probably damaging	1.00
R5576:Apob	UTSW	12	8,048,662 (GRCm39)	missense	probably damaging	1.00
R5582:Apob	UTSW	12	8,060,788 (GRCm39)	missense	probably damaging	1.00
R5629:Apob	UTSW	12	8,057,847 (GRCm39)	missense	probably damaging	1.00
R5678:Apob	UTSW	12	8,041,494 (GRCm39)	missense	possibly damaging	0.92
R5732:Apob	UTSW	12	8,060,353 (GRCm39)	missense	probably benign	0.15
R5734:Apob	UTSW	12	8,038,781 (GRCm39)	missense	probably damaging	1.00
R5742:Apob	UTSW	12	8,057,191 (GRCm39)	missense	probably damaging	1.00
R5751:Apob	UTSW	12	8,062,619 (GRCm39)	nonsense	probably null
R5776:Apob	UTSW	12	8,056,149 (GRCm39)	missense	possibly damaging	0.57
R5778:Apob	UTSW	12	8,065,074 (GRCm39)	missense	probably benign	0.45
R5783:Apob	UTSW	12	8,051,022 (GRCm39)	missense	probably damaging	1.00
R5786:Apob	UTSW	12	8,065,304 (GRCm39)	missense	possibly damaging	0.48
R5837:Apob	UTSW	12	8,053,277 (GRCm39)	missense	probably benign	0.04
R5857:Apob	UTSW	12	8,065,397 (GRCm39)	missense	probably benign	0.00
R6029:Apob	UTSW	12	8,066,243 (GRCm39)	missense	probably damaging	0.99
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6086:Apob	UTSW	12	8,065,164 (GRCm39)	missense	probably benign
R6110:Apob	UTSW	12	8,061,883 (GRCm39)	missense	probably damaging	1.00
R6131:Apob	UTSW	12	8,065,874 (GRCm39)	missense	probably benign	0.17
R6157:Apob	UTSW	12	8,056,077 (GRCm39)	missense	probably benign
R6179:Apob	UTSW	12	8,055,060 (GRCm39)	nonsense	probably null
R6247:Apob	UTSW	12	8,051,801 (GRCm39)	missense	probably damaging	1.00
R6279:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6300:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6320:Apob	UTSW	12	8,039,194 (GRCm39)	missense	probably benign	0.27
R6339:Apob	UTSW	12	8,066,188 (GRCm39)	missense	probably damaging	0.99
R6353:Apob	UTSW	12	8,059,421 (GRCm39)	missense	probably damaging	1.00
R6395:Apob	UTSW	12	8,058,507 (GRCm39)	missense	probably benign	0.45
R6441:Apob	UTSW	12	8,037,796 (GRCm39)	missense	probably damaging	1.00
R6492:Apob	UTSW	12	8,058,261 (GRCm39)	missense	probably damaging	0.99
R6495:Apob	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
R6502:Apob	UTSW	12	8,051,814 (GRCm39)	missense	probably damaging	0.99
R6520:Apob	UTSW	12	8,033,124 (GRCm39)	missense	probably damaging	1.00
R6644:Apob	UTSW	12	8,059,077 (GRCm39)	missense	probably damaging	0.97
R6704:Apob	UTSW	12	8,060,379 (GRCm39)	missense	probably damaging	0.98
R6750:Apob	UTSW	12	8,047,853 (GRCm39)	missense	probably damaging	1.00
R6759:Apob	UTSW	12	8,061,049 (GRCm39)	missense	probably benign	0.06
R6812:Apob	UTSW	12	8,033,062 (GRCm39)	missense	probably damaging	0.98
R6865:Apob	UTSW	12	8,058,847 (GRCm39)	missense	probably benign	0.05
R6873:Apob	UTSW	12	8,065,995 (GRCm39)	missense	probably benign	0.00
R7013:Apob	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
R7067:Apob	UTSW	12	8,059,423 (GRCm39)	missense	probably damaging	1.00
R7084:Apob	UTSW	12	8,059,591 (GRCm39)	missense	probably benign
R7113:Apob	UTSW	12	8,045,539 (GRCm39)	missense	probably damaging	1.00
R7175:Apob	UTSW	12	8,057,034 (GRCm39)	missense	probably benign	0.33
R7196:Apob	UTSW	12	8,033,893 (GRCm39)	missense	possibly damaging	0.90
R7199:Apob	UTSW	12	8,055,072 (GRCm39)	missense	probably damaging	1.00
R7205:Apob	UTSW	12	8,055,087 (GRCm39)	missense	probably damaging	0.98
R7251:Apob	UTSW	12	8,057,037 (GRCm39)	missense	probably damaging	0.98
R7474:Apob	UTSW	12	8,059,185 (GRCm39)	missense	probably benign	0.29
R7484:Apob	UTSW	12	8,056,884 (GRCm39)	nonsense	probably null
R7538:Apob	UTSW	12	8,052,219 (GRCm39)	missense	probably damaging	0.98
R7636:Apob	UTSW	12	8,059,516 (GRCm39)	missense	possibly damaging	0.86
R7646:Apob	UTSW	12	8,059,189 (GRCm39)	missense	probably damaging	0.99
R7793:Apob	UTSW	12	8,058,124 (GRCm39)	missense	probably damaging	0.99
R7836:Apob	UTSW	12	8,051,885 (GRCm39)	missense	possibly damaging	0.72
R7895:Apob	UTSW	12	8,061,933 (GRCm39)	missense	probably benign	0.00
R8005:Apob	UTSW	12	8,059,744 (GRCm39)	missense	probably benign	0.01
R8013:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8014:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8111:Apob	UTSW	12	8,058,801 (GRCm39)	missense	probably benign	0.16
R8117:Apob	UTSW	12	8,056,435 (GRCm39)	missense	probably damaging	0.99
R8226:Apob	UTSW	12	8,059,056 (GRCm39)	missense	probably benign	0.00
R8244:Apob	UTSW	12	8,060,548 (GRCm39)	missense	probably damaging	0.96
R8280:Apob	UTSW	12	8,060,851 (GRCm39)	missense	possibly damaging	0.46
R8310:Apob	UTSW	12	8,059,033 (GRCm39)	missense	probably benign	0.00
R8327:Apob	UTSW	12	8,051,015 (GRCm39)	missense	possibly damaging	0.72
R8329:Apob	UTSW	12	8,061,135 (GRCm39)	missense	probably damaging	0.98
R8331:Apob	UTSW	12	8,051,882 (GRCm39)	missense	probably benign	0.28
R8351:Apob	UTSW	12	8,056,356 (GRCm39)	missense	probably benign	0.29
R8412:Apob	UTSW	12	8,058,069 (GRCm39)	missense	probably benign	0.33
R8425:Apob	UTSW	12	8,038,842 (GRCm39)	missense	possibly damaging	0.70
R8481:Apob	UTSW	12	8,044,807 (GRCm39)	splice site	probably null
R8493:Apob	UTSW	12	8,059,009 (GRCm39)	missense	possibly damaging	0.87
R8529:Apob	UTSW	12	8,057,353 (GRCm39)	missense	probably damaging	1.00
R8554:Apob	UTSW	12	8,037,830 (GRCm39)	missense	probably damaging	0.98
R8692:Apob	UTSW	12	8,058,270 (GRCm39)	missense	probably damaging	0.98
R8695:Apob	UTSW	12	8,057,830 (GRCm39)	missense	probably damaging	1.00
R8977:Apob	UTSW	12	8,065,990 (GRCm39)	missense	probably damaging	0.99
R9016:Apob	UTSW	12	8,035,408 (GRCm39)	splice site	silent
R9020:Apob	UTSW	12	8,063,999 (GRCm39)	missense	probably damaging	1.00
R9037:Apob	UTSW	12	8,066,501 (GRCm39)	missense	probably benign	0.15
R9053:Apob	UTSW	12	8,058,954 (GRCm39)	missense	possibly damaging	0.72
R9062:Apob	UTSW	12	8,058,046 (GRCm39)	missense	possibly damaging	0.91
R9142:Apob	UTSW	12	8,062,705 (GRCm39)	missense	possibly damaging	0.95
R9180:Apob	UTSW	12	8,047,925 (GRCm39)	missense	probably damaging	1.00
R9205:Apob	UTSW	12	8,030,635 (GRCm39)	missense	probably damaging	0.99
R9248:Apob	UTSW	12	8,065,231 (GRCm39)	nonsense	probably null
R9277:Apob	UTSW	12	8,061,183 (GRCm39)	missense	probably benign	0.01
R9305:Apob	UTSW	12	8,058,053 (GRCm39)	missense	probably benign	0.04
R9358:Apob	UTSW	12	8,060,833 (GRCm39)	missense	probably benign	0.14
R9375:Apob	UTSW	12	8,029,261 (GRCm39)	missense	possibly damaging	0.91
R9385:Apob	UTSW	12	8,056,399 (GRCm39)	missense	possibly damaging	0.91
R9386:Apob	UTSW	12	8,056,629 (GRCm39)	missense	probably damaging	0.99
R9392:Apob	UTSW	12	8,057,098 (GRCm39)	missense	probably benign	0.45
R9470:Apob	UTSW	12	8,039,219 (GRCm39)	missense	possibly damaging	0.94
R9523:Apob	UTSW	12	8,052,069 (GRCm39)	missense	probably damaging	1.00
R9545:Apob	UTSW	12	8,033,890 (GRCm39)	missense	possibly damaging	0.81
R9629:Apob	UTSW	12	8,059,054 (GRCm39)	missense	probably damaging	1.00
R9702:Apob	UTSW	12	8,057,559 (GRCm39)	missense	probably damaging	0.96
R9703:Apob	UTSW	12	8,030,507 (GRCm39)	missense	probably damaging	0.99
R9719:Apob	UTSW	12	8,065,464 (GRCm39)	missense	probably benign	0.15
R9726:Apob	UTSW	12	8,056,926 (GRCm39)	missense	probably damaging	0.99
R9729:Apob	UTSW	12	8,066,125 (GRCm39)	missense	probably damaging	0.99
X0027:Apob	UTSW	12	8,057,975 (GRCm39)	missense	probably benign
Z1088:Apob	UTSW	12	8,062,936 (GRCm39)	missense	possibly damaging	0.95
Z1088:Apob	UTSW	12	8,055,945 (GRCm39)	nonsense	probably null
Z1088:Apob	UTSW	12	8,055,074 (GRCm39)	missense	possibly damaging	0.91
Z1176:Apob	UTSW	12	8,054,978 (GRCm39)	missense	probably benign	0.00
Z1176:Apob	UTSW	12	8,048,011 (GRCm39)	missense	probably damaging	1.00
Z1177:Apob	UTSW	12	8,065,249 (GRCm39)	frame shift	probably null
Z1177:Apob	UTSW	12	8,038,765 (GRCm39)	missense	probably benign	0.43

Predicted Primers

PCR Primer
(F):5'- GATGTGCACAGACCTTGAACC -3'
(R):5'- CTGAGCTCCTTCAGTCACAC -3'

Sequencing Primer
(F):5'- TGGGCCATATAAGTCCATATACC -3'
(R):5'- CTTACCTCAAAGAGATCAAGTGAAG -3'

Posted On

2019-11-26