Mutagenetix > Incidental Mutation

Incidental Mutation 'R7787:Muc4'

599625

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

045843-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R7787 (G1)

Quality Score

163.009

Status

Validated

Chromosome

Chromosomal Location

32555089-32602761 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 32575221 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Glutamic Acid at position 1269 (Q1269E)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000132475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096106
AA Change: Q1269E

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: Q1269E

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132475

SMART Domains

Protein: ENSMUSP00000119029
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	38	45	N/A	INTRINSIC
low complexity region	72	84	N/A	INTRINSIC
low complexity region	99	127	N/A	INTRINSIC
low complexity region	148	173	N/A	INTRINSIC
low complexity region	192	224	N/A	INTRINSIC
low complexity region	283	293	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	T	A	7: 27,263,926 (GRCm39)	I38N	probably damaging	Het
Abcb4	G	A	5: 8,959,220 (GRCm39)	V216M	probably damaging	Het
Abcc2	G	A	19: 43,772,685 (GRCm39)	V32M	probably damaging	Het
Adgrb3	C	A	1: 25,471,625 (GRCm39)	V714F	probably damaging	Het
Ahnak	T	A	19: 8,986,679 (GRCm39)	D2654E	unknown	Het
Ak8	T	A	2: 28,602,324 (GRCm39)	I86N	probably damaging	Het
Apob	G	A	12: 8,040,780 (GRCm39)	R635Q	probably damaging	Het
Atp10b	G	A	11: 43,150,700 (GRCm39)	R1466H	possibly damaging	Het
Bltp1	A	C	3: 36,939,557 (GRCm39)	H137P	probably damaging	Het
Btnl9	T	C	11: 49,066,866 (GRCm39)	T252A	unknown	Het
Cacna1e	T	A	1: 154,358,314 (GRCm39)	I648F	probably damaging	Het
Camkk1	A	G	11: 72,917,412 (GRCm39)	D121G	probably benign	Het
Cc2d1a	C	A	8: 84,860,144 (GRCm39)	Q904H	possibly damaging	Het
Cd1d1	A	G	3: 86,904,903 (GRCm39)	S212P	probably damaging	Het
Cfap69	C	T	5: 5,639,260 (GRCm39)	C638Y	probably damaging	Het
Cilk1	G	C	9: 78,074,902 (GRCm39)	V586L	probably benign	Het
Clca4a	A	G	3: 144,659,594 (GRCm39)	V754A	probably benign	Het
Cnpy4	T	A	5: 138,190,900 (GRCm39)	H187Q	probably benign	Het
Cpne5	A	T	17: 29,407,261 (GRCm39)		probably null	Het
Crnkl1	A	T	2: 145,767,515 (GRCm39)	N359K	probably benign	Het
Cyb561	A	T	11: 105,828,466 (GRCm39)	L63H	probably damaging	Het
Dock4	A	G	12: 40,775,676 (GRCm39)	T540A	probably benign	Het
Edc4	T	A	8: 106,614,146 (GRCm39)	Y7*	probably null	Het
Fstl5	A	C	3: 76,337,131 (GRCm39)	D230A	probably damaging	Het
Fthl17b	C	T	X: 8,829,043 (GRCm39)	R9Q	possibly damaging	Het
Fthl17b	C	T	X: 8,829,047 (GRCm39)	V8M	possibly damaging	Het
H2bw2	G	A	X: 135,828,471 (GRCm39)	R120K	unknown	Het
Hecw1	T	C	13: 14,493,494 (GRCm39)	Q337R	probably damaging	Het
Hmcn1	A	T	1: 150,632,343 (GRCm39)	Y865N	probably damaging	Het
Hyal6	A	G	6: 24,743,735 (GRCm39)	Y477C	probably damaging	Het
Ifi205	C	T	1: 173,842,640 (GRCm39)	G352E	probably damaging	Het
Ifi205	T	A	1: 173,842,644 (GRCm39)	S351C	probably damaging	Het
Irak3	C	A	10: 120,012,256 (GRCm39)	Q169H	probably benign	Het
Itgb1	T	A	8: 129,453,499 (GRCm39)	N99K	probably benign	Het
Kng2	A	G	16: 22,818,598 (GRCm39)	F298S	probably damaging	Het
Kri1	T	C	9: 21,192,380 (GRCm39)	E256G		Het
Lamc3	T	C	2: 31,790,551 (GRCm39)	I257T	probably damaging	Het
Mas1	A	C	17: 13,061,374 (GRCm39)	N16K	possibly damaging	Het
Mdn1	T	A	4: 32,741,794 (GRCm39)	L3855Q	probably damaging	Het
Mybph	G	A	1: 134,125,246 (GRCm39)	G258D	probably benign	Het
Mycbp2	A	C	14: 103,364,533 (GRCm39)	H4358Q	probably damaging	Het
Nat10	T	C	2: 103,552,208 (GRCm39)	D1012G	unknown	Het
Nub1	C	A	5: 24,913,801 (GRCm39)	Q561K	probably benign	Het
Nynrin	A	G	14: 56,107,980 (GRCm39)	N1029S	probably benign	Het
Or52x1	A	T	7: 104,853,252 (GRCm39)	C99*	probably null	Het
Or56a4	T	G	7: 104,806,401 (GRCm39)	I163L	probably benign	Het
Or5k15	A	G	16: 58,709,953 (GRCm39)	F210S	probably benign	Het
Or6e1	A	G	14: 54,520,169 (GRCm39)	L61P	probably damaging	Het
Or8g19	C	T	9: 39,055,548 (GRCm39)	L51F	probably benign	Het
Pcsk1	T	A	13: 75,280,277 (GRCm39)	Y701N	possibly damaging	Het
Pglyrp4	C	G	3: 90,640,295 (GRCm39)	H182D	probably damaging	Het
Phaf1	T	G	8: 105,957,820 (GRCm39)	V42G	probably damaging	Het
Pkp4	C	A	2: 59,152,881 (GRCm39)	D576E	probably damaging	Het
Plec	A	G	15: 76,083,811 (GRCm39)	V17A	unknown	Het
Polq	T	A	16: 36,837,671 (GRCm39)	N194K	probably damaging	Het
Pou4f1	A	T	14: 104,703,460 (GRCm39)	M324K	unknown	Het
Ppa2	G	T	3: 133,036,259 (GRCm39)	G138W	probably damaging	Het
Prob1	A	T	18: 35,785,285 (GRCm39)	F990I	possibly damaging	Het
Proser1	T	A	3: 53,380,969 (GRCm39)	I182N	probably damaging	Het
Sgk3	T	C	1: 9,952,016 (GRCm39)	L214P	probably damaging	Het
Sipa1l1	T	C	12: 82,496,762 (GRCm39)	S1765P	possibly damaging	Het
Slc15a2	G	A	16: 36,572,228 (GRCm39)	S712L	probably benign	Het
Slc38a9	T	C	13: 112,825,880 (GRCm39)	L106P	probably damaging	Het
Slc43a2	G	A	11: 75,453,900 (GRCm39)	R271H	probably damaging	Het
Spsb4	T	A	9: 96,877,643 (GRCm39)	I227F	probably damaging	Het
Srgap3	C	T	6: 112,752,520 (GRCm39)	M321I	probably benign	Het
Stam2	T	C	2: 52,596,418 (GRCm39)	I340V	probably benign	Het
Tbc1d5	T	A	17: 51,181,711 (GRCm39)	R341*	probably null	Het
Terf2ip	A	C	8: 112,742,087 (GRCm39)	E260D	probably damaging	Het
Tert	G	T	13: 73,797,051 (GRCm39)	K1096N	probably damaging	Het
Ttc32	T	A	12: 9,088,083 (GRCm39)	D103E	probably benign	Het
Ube3d	T	A	9: 86,254,395 (GRCm39)	Q362L	possibly damaging	Het
Unc5c	G	A	3: 141,474,313 (GRCm39)	G295D	probably damaging	Het
Wnt7b	T	A	15: 85,428,112 (GRCm39)	I117F	probably damaging	Het
Zcchc7	T	C	4: 44,895,043 (GRCm39)		probably null	Het
Zfp619	A	G	7: 39,186,226 (GRCm39)	Q752R	possibly damaging	Het
Zkscan3	T	C	13: 21,572,034 (GRCm39)	K533E	possibly damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,599,186 (GRCm39)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,574,613 (GRCm39)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,569,728 (GRCm39)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,575,465 (GRCm39)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,140 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,139 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,114 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,576,179 (GRCm39)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,575,246 (GRCm39)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,575,362 (GRCm39)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,584,340 (GRCm39)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,569,473 (GRCm39)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,577,001 (GRCm39)	missense	unknown
IGL01625:Muc4	APN	16	32,575,918 (GRCm39)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,555,220 (GRCm39)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,581,722 (GRCm39)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,574,624 (GRCm39)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,570,244 (GRCm39)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,575,383 (GRCm39)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,598,023 (GRCm39)	splice site	probably benign
IGL02210:Muc4	APN	16	32,574,054 (GRCm39)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,569,668 (GRCm39)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,570,894 (GRCm39)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,576,503 (GRCm39)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,596,193 (GRCm39)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,575,656 (GRCm39)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,570,466 (GRCm39)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,569,212 (GRCm39)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,570,866 (GRCm39)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,570,766 (GRCm39)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,570,257 (GRCm39)	nonsense	probably null
IGL03335:Muc4	APN	16	32,574,449 (GRCm39)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,575,436 (GRCm39)	missense	probably benign	0.01
multisplendored	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,590,768 (GRCm39)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,580,625 (GRCm39)	splice site	probably benign
BB004:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
BB014:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
IGL02835:Muc4	UTSW	16	32,584,319 (GRCm39)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,580,622 (GRCm39)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,575,542 (GRCm39)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,576,391 (GRCm39)	missense	unknown
R0119:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,591,978 (GRCm39)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,586,120 (GRCm39)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,574,333 (GRCm39)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,582,910 (GRCm39)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,569,887 (GRCm39)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,577,120 (GRCm39)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,570,925 (GRCm39)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,570,488 (GRCm39)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,590,201 (GRCm39)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,590,221 (GRCm39)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,599,329 (GRCm39)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,576,570 (GRCm39)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,569,343 (GRCm39)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,574,448 (GRCm39)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,574,033 (GRCm39)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,569,167 (GRCm39)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,575,875 (GRCm39)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,577,276 (GRCm39)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,576,307 (GRCm39)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,576,625 (GRCm39)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,576,715 (GRCm39)	missense	unknown
R1928:Muc4	UTSW	16	32,569,350 (GRCm39)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,569,460 (GRCm39)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,570,121 (GRCm39)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,574,054 (GRCm39)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,576,783 (GRCm39)	missense	unknown
R2121:Muc4	UTSW	16	32,580,612 (GRCm39)	missense	unknown
R2139:Muc4	UTSW	16	32,581,599 (GRCm39)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,569,294 (GRCm39)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,587,316 (GRCm39)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,576,265 (GRCm39)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,577,846 (GRCm39)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,575,647 (GRCm39)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,576,200 (GRCm39)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,590,666 (GRCm39)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,577,075 (GRCm39)	missense	unknown
R4523:Muc4	UTSW	16	32,555,154 (GRCm39)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,576,217 (GRCm39)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,577,432 (GRCm39)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,589,651 (GRCm39)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,576,220 (GRCm39)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,570,032 (GRCm39)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,596,277 (GRCm39)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,574,916 (GRCm39)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,570,565 (GRCm39)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,576,472 (GRCm39)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,575,437 (GRCm39)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,575,332 (GRCm39)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,576,670 (GRCm39)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,577,432 (GRCm39)	nonsense	probably null
R5161:Muc4	UTSW	16	32,582,895 (GRCm39)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,570,556 (GRCm39)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,570,484 (GRCm39)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,581,635 (GRCm39)	missense	unknown
R5567:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,569,600 (GRCm39)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,597,181 (GRCm39)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,555,059 (GRCm39)	nonsense	probably null
R5708:Muc4	UTSW	16	32,575,515 (GRCm39)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,570,734 (GRCm39)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,595,213 (GRCm39)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,570,113 (GRCm39)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,570,523 (GRCm39)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,574,480 (GRCm39)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,576,652 (GRCm39)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,579,682 (GRCm39)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,596,157 (GRCm39)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,577,169 (GRCm39)	missense	unknown
R6144:Muc4	UTSW	16	32,587,298 (GRCm39)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,554,958 (GRCm39)	start gained	probably benign
R6268:Muc4	UTSW	16	32,589,141 (GRCm39)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,570,853 (GRCm39)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,575,237 (GRCm39)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,575,476 (GRCm39)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,587,725 (GRCm39)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,555,061 (GRCm39)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,599,320 (GRCm39)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,570,607 (GRCm39)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,579,694 (GRCm39)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,602,378 (GRCm39)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,587,312 (GRCm39)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,596,177 (GRCm39)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,582,892 (GRCm39)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,570,817 (GRCm39)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,570,628 (GRCm39)	nonsense	probably null
R7033:Muc4	UTSW	16	32,576,698 (GRCm39)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,577,465 (GRCm39)	missense
R7123:Muc4	UTSW	16	32,569,509 (GRCm39)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,582,862 (GRCm39)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,576,835 (GRCm39)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,575,710 (GRCm39)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,575,740 (GRCm39)	missense	unknown
R7381:Muc4	UTSW	16	32,601,285 (GRCm39)	missense
R7411:Muc4	UTSW	16	32,570,140 (GRCm39)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,587,324 (GRCm39)	missense
R7539:Muc4	UTSW	16	32,576,770 (GRCm39)	missense
R7544:Muc4	UTSW	16	32,555,016 (GRCm39)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,586,076 (GRCm39)	missense
R7596:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,574,161 (GRCm39)	nonsense	probably null
R7639:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,580,479 (GRCm39)	missense
R7651:Muc4	UTSW	16	32,576,949 (GRCm39)	missense
R7688:Muc4	UTSW	16	32,570,278 (GRCm39)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,574,439 (GRCm39)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,589,108 (GRCm39)	missense
R7763:Muc4	UTSW	16	32,574,739 (GRCm39)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,576,568 (GRCm39)	missense	unknown
R7789:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,570,139 (GRCm39)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
R7930:Muc4	UTSW	16	32,575,369 (GRCm39)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,577,123 (GRCm39)	missense
R8096:Muc4	UTSW	16	32,575,764 (GRCm39)	missense	unknown
R8115:Muc4	UTSW	16	32,575,678 (GRCm39)	missense	unknown
R8162:Muc4	UTSW	16	32,569,197 (GRCm39)	missense	unknown
R8220:Muc4	UTSW	16	32,575,701 (GRCm39)	missense	unknown
R8260:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,575,434 (GRCm39)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,576,271 (GRCm39)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,574,201 (GRCm39)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,574,799 (GRCm39)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,574,421 (GRCm39)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,575,629 (GRCm39)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,602,356 (GRCm39)	missense
R8845:Muc4	UTSW	16	32,576,889 (GRCm39)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,569,461 (GRCm39)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,570,280 (GRCm39)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,575,285 (GRCm39)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,574,009 (GRCm39)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,582,910 (GRCm39)	missense
R9034:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,577,842 (GRCm39)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,589,102 (GRCm39)	missense
R9336:Muc4	UTSW	16	32,569,756 (GRCm39)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,569,971 (GRCm39)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,576,992 (GRCm39)	missense
R9429:Muc4	UTSW	16	32,576,098 (GRCm39)	missense	unknown
R9570:Muc4	UTSW	16	32,569,695 (GRCm39)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,577,234 (GRCm39)	missense
R9696:Muc4	UTSW	16	32,574,712 (GRCm39)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,590,644 (GRCm39)	missense
R9743:Muc4	UTSW	16	32,601,194 (GRCm39)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,570,676 (GRCm39)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,570,643 (GRCm39)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,575,855 (GRCm39)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,579,693 (GRCm39)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,576,450 (GRCm39)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,587,767 (GRCm39)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,595,236 (GRCm39)	missense
Z1177:Muc4	UTSW	16	32,587,768 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ATCCCAGAACATGTCAACAGTG -3'
(R):5'- CTCATGAGGGAGCATGTGAG -3'

Sequencing Primer
(F):5'- GAACATGTCAACAGTGTCAGC -3'
(R):5'- TTCTGGGAGGAAGGTGAGACC -3'

Posted On

2019-11-26