Mutagenetix > Incidental Mutation

Incidental Mutation 'R7793:Eif4g1'

600080

Institutional Source

Beutler Lab

Gene Symbol

Eif4g1

Ensembl Gene

ENSMUSG00000045983

Gene Name

eukaryotic translation initiation factor 4, gamma 1

Synonyms

E030015G23Rik

MMRRC Submission

045849-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.970) question?

Stock #

R7793 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20491457-20511633 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 20507364 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 1413 (V1413I)

Ref Sequence

ENSEMBL: ENSMUSP00000047678 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044783] [ENSMUST00000073840] [ENSMUST00000115457] [ENSMUST00000115460] [ENSMUST00000115461] [ENSMUST00000115463] [ENSMUST00000118919] [ENSMUST00000128594] [ENSMUST00000142344] [ENSMUST00000143939] [ENSMUST00000149543] [ENSMUST00000150333] [ENSMUST00000156226] [ENSMUST00000232207]

AlphaFold

Q6NZJ6

Predicted Effect

probably benign
Transcript: ENSMUST00000044783
AA Change: V1413I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000047678
Gene: ENSMUSG00000045983
AA Change: V1413I

Domain	Start	End	E-Value	Type
low complexity region	60	81	N/A	INTRINSIC
PDB:1LJ2\|D	179	206	1e-10	PDB
low complexity region	260	286	N/A	INTRINSIC
low complexity region	436	457	N/A	INTRINSIC
low complexity region	464	487	N/A	INTRINSIC
Blast:MIF4G	638	683	7e-9	BLAST
low complexity region	685	707	N/A	INTRINSIC
MIF4G	765	993	5.14e-72	SMART
low complexity region	1035	1047	N/A	INTRINSIC
low complexity region	1092	1106	N/A	INTRINSIC
low complexity region	1157	1178	N/A	INTRINSIC
low complexity region	1186	1201	N/A	INTRINSIC
MA3	1242	1354	3.83e-39	SMART
low complexity region	1441	1452	N/A	INTRINSIC
eIF5C	1508	1595	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000073840
AA Change: V1406I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000073506
Gene: ENSMUSG00000045983
AA Change: V1406I

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	1e-10	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	676	7e-9	BLAST
low complexity region	678	700	N/A	INTRINSIC
MIF4G	758	986	5.14e-72	SMART
low complexity region	1028	1040	N/A	INTRINSIC
low complexity region	1085	1099	N/A	INTRINSIC
low complexity region	1150	1171	N/A	INTRINSIC
low complexity region	1179	1194	N/A	INTRINSIC
MA3	1235	1347	3.83e-39	SMART
low complexity region	1434	1445	N/A	INTRINSIC
eIF5C	1501	1588	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115457
AA Change: V1366I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000111117
Gene: ENSMUSG00000045983
AA Change: V1366I

Domain	Start	End	E-Value	Type
low complexity region	13	34	N/A	INTRINSIC
PDB:1LJ2\|D	132	159	9e-11	PDB
low complexity region	213	239	N/A	INTRINSIC
low complexity region	389	410	N/A	INTRINSIC
low complexity region	417	440	N/A	INTRINSIC
Blast:MIF4G	591	636	7e-9	BLAST
low complexity region	638	660	N/A	INTRINSIC
MIF4G	718	946	5.14e-72	SMART
low complexity region	988	1000	N/A	INTRINSIC
low complexity region	1045	1059	N/A	INTRINSIC
low complexity region	1110	1131	N/A	INTRINSIC
low complexity region	1139	1154	N/A	INTRINSIC
MA3	1195	1307	3.83e-39	SMART
low complexity region	1394	1405	N/A	INTRINSIC
eIF5C	1461	1548	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115460
AA Change: V1413I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000111120
Gene: ENSMUSG00000045983
AA Change: V1413I

Domain	Start	End	E-Value	Type
low complexity region	60	81	N/A	INTRINSIC
PDB:1LJ2\|D	179	206	1e-10	PDB
low complexity region	260	286	N/A	INTRINSIC
low complexity region	436	457	N/A	INTRINSIC
low complexity region	464	487	N/A	INTRINSIC
Blast:MIF4G	638	683	7e-9	BLAST
low complexity region	685	707	N/A	INTRINSIC
MIF4G	765	993	5.14e-72	SMART
low complexity region	1035	1047	N/A	INTRINSIC
low complexity region	1092	1106	N/A	INTRINSIC
low complexity region	1157	1178	N/A	INTRINSIC
low complexity region	1186	1201	N/A	INTRINSIC
MA3	1242	1354	3.83e-39	SMART
low complexity region	1441	1452	N/A	INTRINSIC
eIF5C	1508	1595	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115461
AA Change: V1407I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000111121
Gene: ENSMUSG00000045983
AA Change: V1407I

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	1e-10	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	676	8e-9	BLAST
low complexity region	678	693	N/A	INTRINSIC
MIF4G	759	987	5.14e-72	SMART
low complexity region	1029	1041	N/A	INTRINSIC
low complexity region	1086	1100	N/A	INTRINSIC
low complexity region	1151	1172	N/A	INTRINSIC
low complexity region	1180	1195	N/A	INTRINSIC
MA3	1236	1348	3.83e-39	SMART
low complexity region	1435	1446	N/A	INTRINSIC
eIF5C	1502	1589	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115463
AA Change: V1399I

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000111123
Gene: ENSMUSG00000045983
AA Change: V1399I

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	1e-10	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	676	7e-9	BLAST
low complexity region	678	700	N/A	INTRINSIC
MIF4G	758	986	5.14e-72	SMART
low complexity region	1030	1036	N/A	INTRINSIC
low complexity region	1078	1092	N/A	INTRINSIC
low complexity region	1143	1164	N/A	INTRINSIC
low complexity region	1172	1187	N/A	INTRINSIC
MA3	1228	1340	3.83e-39	SMART
low complexity region	1427	1438	N/A	INTRINSIC
eIF5C	1494	1581	3.78e-33	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000118919

SMART Domains

Protein: ENSMUSP00000113719
Gene: ENSMUSG00000050821

Domain	Start	End	E-Value	Type
Pfam:FAM131	1	271	4e-119	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000128594

SMART Domains

Protein: ENSMUSP00000144594
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	8	35	5e-11	PDB
low complexity region	89	115	N/A	INTRINSIC
low complexity region	265	286	N/A	INTRINSIC
low complexity region	293	316	N/A	INTRINSIC
Blast:MIF4G	467	512	4e-9	BLAST
low complexity region	514	536	N/A	INTRINSIC
MIF4G	594	795	1.1e-52	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000142344

SMART Domains

Protein: ENSMUSP00000116029
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC
PDB:1LJ2\|D	172	199	5e-11	PDB
low complexity region	253	279	N/A	INTRINSIC
low complexity region	429	450	N/A	INTRINSIC
low complexity region	457	480	N/A	INTRINSIC
Blast:MIF4G	631	672	6e-8	BLAST
low complexity region	678	693	N/A	INTRINSIC
MIF4G	759	958	5.49e-49	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143939
AA Change: V1116I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000144320
Gene: ENSMUSG00000045983
AA Change: V1116I

Domain	Start	End	E-Value	Type
low complexity region	139	160	N/A	INTRINSIC
low complexity region	167	190	N/A	INTRINSIC
Blast:MIF4G	341	386	6e-9	BLAST
low complexity region	388	410	N/A	INTRINSIC
MIF4G	468	696	2.2e-74	SMART
low complexity region	738	750	N/A	INTRINSIC
low complexity region	795	809	N/A	INTRINSIC
low complexity region	860	881	N/A	INTRINSIC
low complexity region	889	904	N/A	INTRINSIC
MA3	945	1057	1.7e-41	SMART
low complexity region	1144	1155	N/A	INTRINSIC
eIF5C	1211	1298	1.8e-35	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000149543

Predicted Effect

probably benign
Transcript: ENSMUST00000150333

SMART Domains

Protein: ENSMUSP00000144107
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
PDB:1LJ2\|D	113	140	5e-11	PDB
low complexity region	194	220	N/A	INTRINSIC
low complexity region	370	391	N/A	INTRINSIC
low complexity region	398	421	N/A	INTRINSIC
Blast:MIF4G	572	613	9e-8	BLAST
low complexity region	619	641	N/A	INTRINSIC
MIF4G	699	900	1.1e-52	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000156226

SMART Domains

Protein: ENSMUSP00000119215
Gene: ENSMUSG00000045983

Domain	Start	End	E-Value	Type
low complexity region	53	74	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000231598
AA Change: R55H

Predicted Effect

probably benign
Transcript: ENSMUST00000232207

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (94/94)

MGI Phenotype

FUNCTION: This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]
PHENOTYPE: Mice homozygous for an amino acid substitution (R1207H) are viable and fertile. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2200002J24Rik	T	G	7: 30,399,368 (GRCm39)	I54S	probably damaging	Het
2310002L09Rik	C	T	4: 73,861,172 (GRCm39)	V143M	probably benign	Het
Abca1	T	C	4: 53,042,367 (GRCm39)	I1902V	not run	Het
Apob	A	C	12: 8,058,124 (GRCm39)	D2202A	probably damaging	Het
Arhgap44	T	A	11: 64,900,750 (GRCm39)	S623C	probably damaging	Het
Arhgef7	T	A	8: 11,874,507 (GRCm39)	W713R	possibly damaging	Het
Axdnd1	A	C	1: 156,166,313 (GRCm39)		probably null	Het
Bcl9l	C	A	9: 44,420,994 (GRCm39)	H1467N	probably damaging	Het
Bcl9l	T	C	9: 44,420,263 (GRCm39)	M1223T	probably benign	Het
Bltp2	C	T	11: 78,164,031 (GRCm39)	P1124L	possibly damaging	Het
Brinp3	A	G	1: 146,622,306 (GRCm39)	N236S	probably benign	Het
Cckbr	T	G	7: 105,082,798 (GRCm39)	L54V	probably benign	Het
Cd6	G	A	19: 10,775,722 (GRCm39)	Q246*	probably null	Het
Cep89	G	A	7: 35,129,353 (GRCm39)	R630H	probably damaging	Het
Ces3a	T	A	8: 105,782,293 (GRCm39)		probably null	Het
Cgnl1	T	A	9: 71,632,917 (GRCm39)	N145Y	probably damaging	Het
Col6a5	T	C	9: 105,775,934 (GRCm39)	D1707G	probably damaging	Het
Cyp2w1	A	G	5: 139,341,895 (GRCm39)	T103A	probably damaging	Het
Dip2a	A	T	10: 76,114,417 (GRCm39)	I1021N	probably benign	Het
Dnah2	T	C	11: 69,386,040 (GRCm39)	T981A	probably benign	Het
Dnah8	A	G	17: 31,074,918 (GRCm39)	I4546V	probably benign	Het
E2f8	A	C	7: 48,527,823 (GRCm39)	F106L	probably benign	Het
Ecpas	T	C	4: 58,853,150 (GRCm39)	N395S	probably damaging	Het
Epha10	G	A	4: 124,808,246 (GRCm39)	V688I	probably benign	Het
Exoc2	A	T	13: 31,095,161 (GRCm39)	V245D	probably benign	Het
Eya4	T	A	10: 23,102,714 (GRCm39)	E23D	probably benign	Het
Fbxw10	T	A	11: 62,738,213 (GRCm39)	W36R	possibly damaging	Het
Gabbr1	G	A	17: 37,358,393 (GRCm39)	G44S	probably benign	Het
Gabrg3	T	A	7: 56,829,328 (GRCm39)	Q143L	probably benign	Het
Gm14412	A	C	2: 177,007,660 (GRCm39)	I78M	possibly damaging	Het
Gm7298	T	C	6: 121,737,563 (GRCm39)		probably null	Het
Hpse2	A	T	19: 43,376,509 (GRCm39)	L81Q	probably damaging	Het
Il18r1	A	G	1: 40,510,924 (GRCm39)	H3R	probably benign	Het
Kansl1l	T	C	1: 66,817,173 (GRCm39)	K396E	probably damaging	Het
Kbtbd3	T	A	9: 4,331,221 (GRCm39)	S532T	probably damaging	Het
Lrrtm4	T	C	6: 79,999,841 (GRCm39)	Y418H	probably damaging	Het
Mfng	G	A	15: 78,657,265 (GRCm39)	R70C	probably damaging	Het
Mx2	T	A	16: 97,348,083 (GRCm39)	I236K	probably damaging	Het
Mysm1	T	C	4: 94,853,369 (GRCm39)	Q410R	probably damaging	Het
Nlrp12	T	C	7: 3,294,030 (GRCm39)	N100S	probably benign	Het
Nlrp4b	T	A	7: 10,459,001 (GRCm39)	H773Q	probably benign	Het
Nlrp5	T	A	7: 23,123,343 (GRCm39)	S735T	possibly damaging	Het
Nt5c2	G	A	19: 46,880,020 (GRCm39)	R363W	probably benign	Het
Or12k8	A	G	2: 36,974,933 (GRCm39)	F276L	possibly damaging	Het
Or3a10	T	A	11: 73,935,614 (GRCm39)	H162L	possibly damaging	Het
Otud7b	A	T	3: 96,062,528 (GRCm39)	Y589F	probably benign	Het
P2rx1	T	A	11: 72,900,079 (GRCm39)	C165*	probably null	Het
Pax8	T	C	2: 24,319,609 (GRCm39)	D354G	possibly damaging	Het
Pdcd11	G	A	19: 47,094,871 (GRCm39)	V552I	probably benign	Het
Pde6c	G	T	19: 38,148,201 (GRCm39)	C485F	possibly damaging	Het
Pex5	T	C	6: 124,376,300 (GRCm39)	Y434C	probably benign	Het
Ppargc1a	T	C	5: 51,619,851 (GRCm39)		probably null	Het
Prss2	A	T	6: 41,501,886 (GRCm39)	D224V	possibly damaging	Het
Psd2	A	G	18: 36,136,032 (GRCm39)	N440S	probably benign	Het
Psmb3	T	A	11: 97,603,265 (GRCm39)	D159E	probably benign	Het
Ptgr2	T	G	12: 84,354,575 (GRCm39)	L252R	probably damaging	Het
Ptpro	A	T	6: 137,393,818 (GRCm39)	N829Y	probably damaging	Het
Rhobtb2	C	A	14: 70,034,280 (GRCm39)	R315L	probably benign	Het
Rimbp2	A	T	5: 128,866,759 (GRCm39)	V520D	possibly damaging	Het
Schip1	A	T	3: 68,401,911 (GRCm39)	Y24F	probably benign	Het
Sdha	A	T	13: 74,479,555 (GRCm39)	V432E	probably damaging	Het
Sez6	T	C	11: 77,868,426 (GRCm39)	L929P	probably damaging	Het
Shcbp1l	A	G	1: 153,323,571 (GRCm39)	I466V	probably benign	Het
Skor1	T	C	9: 63,052,167 (GRCm39)	S601G	probably damaging	Het
Slc6a7	A	C	18: 61,138,851 (GRCm39)	L219R	probably damaging	Het
Smok2a	T	C	17: 13,444,513 (GRCm39)	M30T	possibly damaging	Het
Sp8	A	G	12: 118,813,144 (GRCm39)	D333G	probably damaging	Het
Spp1	G	A	5: 104,588,200 (GRCm39)	D201N	probably damaging	Het
Sstr3	A	T	15: 78,424,588 (GRCm39)	L53Q	probably damaging	Het
Tacc2	T	A	7: 130,224,843 (GRCm39)	N509K	probably benign	Het
Tdrd1	T	C	19: 56,852,809 (GRCm39)	V1030A	probably damaging	Het
Tfrc	A	G	16: 32,437,985 (GRCm39)	K346R	probably benign	Het
Tgm3	G	A	2: 129,854,330 (GRCm39)		probably null	Het
Tomm5	C	A	4: 45,106,651 (GRCm39)	L88F	unknown	Het
Trak1	T	A	9: 121,245,264 (GRCm39)	C46*	probably null	Het
Trim8	A	T	19: 46,504,053 (GRCm39)	D535V	probably damaging	Het
Trpm7	A	T	2: 126,665,995 (GRCm39)	Y870*	probably null	Het
Tspoap1	T	C	11: 87,655,136 (GRCm39)	L286P	probably benign	Het
Ttll3	AAGTA	AAGTAGAGTA	6: 113,376,120 (GRCm39)		probably null	Het
Ttn	T	A	2: 76,562,389 (GRCm39)	I28819F	probably damaging	Het
Twf2	T	C	9: 106,089,079 (GRCm39)	S89P	probably damaging	Het
Ulk4	C	T	9: 121,092,734 (GRCm39)	E168K	possibly damaging	Het
Unc13b	T	A	4: 43,172,737 (GRCm39)	N1188K	unknown	Het
Utp6	C	T	11: 79,828,556 (GRCm39)	V528I	probably benign	Het
Vmn1r78	T	C	7: 11,887,241 (GRCm39)	F284S	probably benign	Het
Vmn2r54	T	A	7: 12,366,196 (GRCm39)	Q246L	probably damaging	Het
Vmn2r87	A	G	10: 130,313,413 (GRCm39)	M451T	probably benign	Het
Vwf	C	T	6: 125,663,483 (GRCm39)	P2808L		Het
Wdr97	T	C	15: 76,243,307 (GRCm39)	L989P		Het
Wwc1	T	C	11: 35,759,936 (GRCm39)	Q693R	probably benign	Het
Zfp582	C	T	7: 6,356,886 (GRCm39)	S233L	probably damaging	Het
Zfp658	T	A	7: 43,224,108 (GRCm39)	H794Q	possibly damaging	Het
Zfp882	A	G	8: 72,666,985 (GRCm39)	D8G	probably damaging	Het

Other mutations in Eif4g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00584:Eif4g1	APN	16	20,505,504 (GRCm39)	intron	probably benign
IGL00707:Eif4g1	APN	16	20,507,764 (GRCm39)	missense	probably damaging	1.00
IGL00950:Eif4g1	APN	16	20,502,378 (GRCm39)	missense	probably damaging	1.00
IGL01397:Eif4g1	APN	16	20,498,425 (GRCm39)	missense	probably damaging	0.98
IGL01657:Eif4g1	APN	16	20,500,966 (GRCm39)	missense	possibly damaging	0.94
IGL01875:Eif4g1	APN	16	20,499,790 (GRCm39)	missense	probably damaging	0.96
IGL02728:Eif4g1	APN	16	20,505,502 (GRCm39)	intron	probably benign
IGL03155:Eif4g1	APN	16	20,511,167 (GRCm39)	missense	probably damaging	1.00
IGL03339:Eif4g1	APN	16	20,499,734 (GRCm39)	missense	possibly damaging	0.72
R0032:Eif4g1	UTSW	16	20,504,648 (GRCm39)	missense	probably damaging	1.00
R0032:Eif4g1	UTSW	16	20,504,648 (GRCm39)	missense	probably damaging	1.00
R0138:Eif4g1	UTSW	16	20,494,095 (GRCm39)	missense	probably damaging	0.99
R0556:Eif4g1	UTSW	16	20,494,544 (GRCm39)	missense	probably damaging	0.99
R0576:Eif4g1	UTSW	16	20,502,818 (GRCm39)	missense	probably damaging	0.98
R1424:Eif4g1	UTSW	16	20,497,692 (GRCm39)	missense	probably benign	0.03
R1469:Eif4g1	UTSW	16	20,498,758 (GRCm39)	missense	possibly damaging	0.86
R1469:Eif4g1	UTSW	16	20,498,758 (GRCm39)	missense	possibly damaging	0.86
R1487:Eif4g1	UTSW	16	20,497,623 (GRCm39)	unclassified	probably benign
R1659:Eif4g1	UTSW	16	20,499,811 (GRCm39)	missense	probably damaging	0.99
R1697:Eif4g1	UTSW	16	20,498,530 (GRCm39)	missense	probably damaging	0.99
R1848:Eif4g1	UTSW	16	20,500,617 (GRCm39)	missense	probably damaging	1.00
R1855:Eif4g1	UTSW	16	20,505,911 (GRCm39)	missense	possibly damaging	0.77
R1865:Eif4g1	UTSW	16	20,497,398 (GRCm39)	missense	probably damaging	0.99
R3001:Eif4g1	UTSW	16	20,511,134 (GRCm39)	missense	probably damaging	1.00
R3002:Eif4g1	UTSW	16	20,511,134 (GRCm39)	missense	probably damaging	1.00
R4402:Eif4g1	UTSW	16	20,497,593 (GRCm39)	unclassified	probably benign
R4477:Eif4g1	UTSW	16	20,497,593 (GRCm39)	unclassified	probably benign
R4478:Eif4g1	UTSW	16	20,497,593 (GRCm39)	unclassified	probably benign
R4479:Eif4g1	UTSW	16	20,497,593 (GRCm39)	unclassified	probably benign
R4480:Eif4g1	UTSW	16	20,497,593 (GRCm39)	unclassified	probably benign
R4623:Eif4g1	UTSW	16	20,500,095 (GRCm39)	unclassified	probably benign
R4658:Eif4g1	UTSW	16	20,504,684 (GRCm39)	missense	possibly damaging	0.78
R4751:Eif4g1	UTSW	16	20,505,265 (GRCm39)	missense	possibly damaging	0.89
R4859:Eif4g1	UTSW	16	20,500,923 (GRCm39)	missense	probably benign	0.44
R5267:Eif4g1	UTSW	16	20,504,283 (GRCm39)	missense	probably damaging	0.99
R5376:Eif4g1	UTSW	16	20,502,577 (GRCm39)	missense	probably damaging	1.00
R5560:Eif4g1	UTSW	16	20,505,645 (GRCm39)	missense	probably benign
R5719:Eif4g1	UTSW	16	20,507,761 (GRCm39)	missense	probably damaging	1.00
R6632:Eif4g1	UTSW	16	20,504,270 (GRCm39)	missense	probably damaging	0.99
R6849:Eif4g1	UTSW	16	20,499,495 (GRCm39)	missense	probably benign	0.08
R7134:Eif4g1	UTSW	16	20,500,252 (GRCm39)	missense	probably damaging	1.00
R7861:Eif4g1	UTSW	16	20,498,452 (GRCm39)	missense	probably benign
R8309:Eif4g1	UTSW	16	20,507,578 (GRCm39)	missense	probably benign	0.19
R8365:Eif4g1	UTSW	16	20,502,277 (GRCm39)	missense	probably damaging	1.00
R8726:Eif4g1	UTSW	16	20,494,232 (GRCm39)	missense	probably damaging	1.00
R8778:Eif4g1	UTSW	16	20,492,196 (GRCm39)	intron	probably benign
R9604:Eif4g1	UTSW	16	20,500,255 (GRCm39)	missense	possibly damaging	0.90
R9658:Eif4g1	UTSW	16	20,502,863 (GRCm39)	missense	probably benign	0.01
R9779:Eif4g1	UTSW	16	20,498,251 (GRCm39)	missense	probably damaging	0.97
X0062:Eif4g1	UTSW	16	20,503,251 (GRCm39)	missense	probably damaging	1.00
X0065:Eif4g1	UTSW	16	20,501,476 (GRCm39)	missense	probably damaging	1.00
Z1176:Eif4g1	UTSW	16	20,492,158 (GRCm39)	intron	probably benign
Z1177:Eif4g1	UTSW	16	20,505,116 (GRCm39)	frame shift	probably null
Z1177:Eif4g1	UTSW	16	20,502,655 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CACTGAGGTGGTAGTGAGTTCC -3'
(R):5'- AAAGGCAAGTGTCCTCTGG -3'

Sequencing Primer
(F):5'- TATTTAATCTAGGCATAGAAGGGTGG -3'
(R):5'- GGCCAGGAGCTTCAGATTCTTC -3'

Posted On

2019-11-26