Mutagenetix > Incidental Mutation

Incidental Mutation 'R7794:Zfp820'

600150

Institutional Source

Beutler Lab

Gene Symbol

Zfp820

Ensembl Gene

ENSMUSG00000069743

Gene Name

zinc finger protein 820

Synonyms

2610036F08Rik

MMRRC Submission

045850-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R7794 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

22035857-22064740 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 22039109 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 73 (V73G)

Ref Sequence

ENSEMBL: ENSMUSP00000081158 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084141]

AlphaFold

A0A3B2W7H5

Predicted Effect

probably damaging
Transcript: ENSMUST00000084141
AA Change: V73G

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000081158
Gene: ENSMUSG00000069743
AA Change: V73G

Domain	Start	End	E-Value	Type
KRAB	13	73	8.77e-20	SMART
ZnF_C2H2	183	205	6.32e-3	SMART
ZnF_C2H2	211	233	2.79e-4	SMART
ZnF_C2H2	239	261	4.3e-5	SMART
ZnF_C2H2	267	289	4.61e-5	SMART
ZnF_C2H2	295	317	1.2e-3	SMART
ZnF_C2H2	323	345	1.22e-4	SMART
ZnF_C2H2	351	373	2.75e-3	SMART
ZnF_C2H2	379	401	2.95e-3	SMART
ZnF_C2H2	407	429	5.29e-5	SMART
ZnF_C2H2	435	457	1.42e-5	SMART
ZnF_C2H2	463	485	1.2e-3	SMART
ZnF_C2H2	491	513	1.13e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (60/60)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	A	T	2: 69,117,022 (GRCm39)	M542K	possibly damaging	Het
Abcc3	T	A	11: 94,249,697 (GRCm39)	I1083F	probably benign	Het
Acsm5	A	G	7: 119,137,352 (GRCm39)		probably benign	Het
Acyp1	G	T	12: 85,335,053 (GRCm39)	A22E	probably benign	Het
Afdn	C	A	17: 14,102,695 (GRCm39)	A1090E	probably damaging	Het
Ahdc1	T	G	4: 132,791,289 (GRCm39)	D843E	possibly damaging	Het
Bahcc1	G	T	11: 120,163,507 (GRCm39)	E602*	probably null	Het
Bora	A	G	14: 99,310,080 (GRCm39)	T470A	possibly damaging	Het
Bzw1	T	A	1: 58,439,959 (GRCm39)	S166T	probably benign	Het
Car13	A	G	3: 14,719,948 (GRCm39)	H120R	probably damaging	Het
Cstf3	G	A	2: 104,420,926 (GRCm39)	⇒1	probably benign	Het
Dip2a	T	C	10: 76,112,459 (GRCm39)	N1080D	probably damaging	Het
Dis3	A	G	14: 99,336,233 (GRCm39)	L91P	probably benign	Het
E2f6	A	G	12: 16,870,370 (GRCm39)	D174G	possibly damaging	Het
Emsy	A	T	7: 98,249,931 (GRCm39)	S785R	probably benign	Het
Fbxl17	A	G	17: 63,663,806 (GRCm39)	I561T	probably damaging	Het
Gabra6	A	T	11: 42,211,868 (GRCm39)		probably null	Het
Gm47996	C	G	1: 151,086,545 (GRCm39)	P209A	possibly damaging	Het
Hcls1	A	G	16: 36,782,426 (GRCm39)	E365G	probably damaging	Het
Hoxa6	T	A	6: 52,183,548 (GRCm39)	T166S	possibly damaging	Het
Hydin	A	G	8: 111,235,715 (GRCm39)	Y1900C	probably damaging	Het
I0C0044D17Rik	T	C	4: 98,708,582 (GRCm39)		probably benign	Het
Ifitm7	T	C	16: 13,801,610 (GRCm39)	T50A	probably benign	Het
Igkv10-95	A	G	6: 68,657,811 (GRCm39)	Q109R	possibly damaging	Het
Il1rap	A	T	16: 26,541,658 (GRCm39)	H633L	probably benign	Het
Ippk	C	T	13: 49,599,818 (GRCm39)	P226S		Het
Kmt2e	G	A	5: 23,669,714 (GRCm39)	G67D	probably damaging	Het
Kpna6	T	C	4: 129,541,844 (GRCm39)	T518A	probably benign	Het
Marchf11	A	G	15: 26,409,284 (GRCm39)	I328V	probably benign	Het
Mbtps1	A	G	8: 120,265,623 (GRCm39)	I308T	probably damaging	Het
Mug1	A	T	6: 121,833,247 (GRCm39)	D284V	possibly damaging	Het
Myo16	A	G	8: 10,619,913 (GRCm39)	K1488R	unknown	Het
Myom2	G	A	8: 15,133,259 (GRCm39)	G384R	probably damaging	Het
Naa16	A	G	14: 79,614,934 (GRCm39)	Y189H	probably damaging	Het
Nav3	G	A	10: 109,524,717 (GRCm39)	A2304V	probably benign	Het
Or12e10	T	A	2: 87,640,818 (GRCm39)	V218E	probably damaging	Het
Or14c46	T	A	7: 85,918,341 (GRCm39)	I219L	probably damaging	Het
Or14j5	A	T	17: 38,161,678 (GRCm39)	Q65L	probably benign	Het
Orc5	A	T	5: 22,738,782 (GRCm39)	Y160N	possibly damaging	Het
Pan2	T	C	10: 128,152,396 (GRCm39)		probably null	Het
Pcdhb20	A	G	18: 37,637,485 (GRCm39)	R4G	probably benign	Het
Poc1b	A	G	10: 98,965,460 (GRCm39)	S130G	possibly damaging	Het
Psme2b	A	G	11: 48,836,683 (GRCm39)	V88A	probably benign	Het
Ptpn13	C	T	5: 103,640,090 (GRCm39)	T183M	probably benign	Het
Ptpn4	T	C	1: 119,653,767 (GRCm39)	E275G	probably damaging	Het
Rab21	A	T	10: 115,134,762 (GRCm39)	L119*	probably null	Het
Rep15	T	A	6: 146,934,638 (GRCm39)	I159N	probably damaging	Het
Rps6kc1	T	C	1: 190,515,825 (GRCm39)	E967G	probably benign	Het
Rptn	G	A	3: 93,303,036 (GRCm39)	R123K	probably benign	Het
Scn11a	A	T	9: 119,594,580 (GRCm39)	V1271D	probably damaging	Het
Scn5a	A	T	9: 119,358,153 (GRCm39)	I696N	probably damaging	Het
Slc25a12	T	C	2: 71,141,852 (GRCm39)	E267G	probably damaging	Het
Slc5a4b	A	T	10: 75,898,133 (GRCm39)	M527K	probably benign	Het
Spata31e4	C	G	13: 50,856,344 (GRCm39)	P661A	probably damaging	Het
Speg	T	A	1: 75,365,514 (GRCm39)	S632T	probably benign	Het
Stard9	G	A	2: 120,534,911 (GRCm39)	G3723S	probably benign	Het
Synrg	G	T	11: 83,910,400 (GRCm39)	M933I	probably benign	Het
Tmem178b	A	T	6: 40,222,551 (GRCm39)	I89F	probably damaging	Het
Tyr	C	T	7: 87,133,028 (GRCm39)		probably null	Het
Usp30	T	A	5: 114,251,033 (GRCm39)	C237*	probably null	Het
Xpo6	G	T	7: 125,760,035 (GRCm39)	T188K	probably damaging	Het
Zfp382	G	A	7: 29,831,035 (GRCm39)	S108N	possibly damaging	Het

Other mutations in Zfp820

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00424:Zfp820	APN	17	22,038,292 (GRCm39)	missense	probably damaging	1.00
IGL00954:Zfp820	APN	17	22,038,860 (GRCm39)	missense	probably damaging	1.00
IGL01088:Zfp820	APN	17	22,040,162 (GRCm39)	nonsense	probably null
IGL03067:Zfp820	APN	17	22,038,801 (GRCm39)	missense	possibly damaging	0.68
R0158:Zfp820	UTSW	17	22,038,800 (GRCm39)	missense	probably benign	0.06
R0617:Zfp820	UTSW	17	22,038,685 (GRCm39)	missense	probably damaging	0.99
R0658:Zfp820	UTSW	17	22,037,901 (GRCm39)	missense	probably benign	0.00
R0696:Zfp820	UTSW	17	22,039,041 (GRCm39)	missense	possibly damaging	0.94
R0791:Zfp820	UTSW	17	22,038,509 (GRCm39)	missense	probably benign	0.05
R0792:Zfp820	UTSW	17	22,038,509 (GRCm39)	missense	probably benign	0.05
R0882:Zfp820	UTSW	17	22,042,817 (GRCm39)	splice site	probably benign
R1421:Zfp820	UTSW	17	22,038,861 (GRCm39)	missense	possibly damaging	0.85
R1573:Zfp820	UTSW	17	22,037,737 (GRCm39)	missense	probably benign	0.00
R1928:Zfp820	UTSW	17	22,038,316 (GRCm39)	missense	probably benign	0.00
R3889:Zfp820	UTSW	17	22,037,877 (GRCm39)	missense	probably benign	0.25
R4782:Zfp820	UTSW	17	22,037,966 (GRCm39)	missense	probably benign	0.00
R4799:Zfp820	UTSW	17	22,037,966 (GRCm39)	missense	probably benign	0.00
R4807:Zfp820	UTSW	17	22,042,853 (GRCm39)	start codon destroyed	probably null	1.00
R4913:Zfp820	UTSW	17	22,038,200 (GRCm39)	missense	probably benign	0.42
R5100:Zfp820	UTSW	17	22,040,054 (GRCm39)	missense	possibly damaging	0.64
R5766:Zfp820	UTSW	17	22,038,983 (GRCm39)	missense	probably damaging	0.96
R5772:Zfp820	UTSW	17	22,037,702 (GRCm39)	missense	probably damaging	1.00
R6516:Zfp820	UTSW	17	22,038,354 (GRCm39)	missense	probably damaging	1.00
R6577:Zfp820	UTSW	17	22,038,384 (GRCm39)	missense	probably benign	0.00
R7094:Zfp820	UTSW	17	22,038,246 (GRCm39)	missense	probably benign	0.41
R7570:Zfp820	UTSW	17	22,037,994 (GRCm39)	missense	probably benign	0.14
R8545:Zfp820	UTSW	17	22,038,438 (GRCm39)	missense	probably damaging	1.00
R8692:Zfp820	UTSW	17	22,037,876 (GRCm39)	missense	probably benign	0.43
R9072:Zfp820	UTSW	17	22,039,031 (GRCm39)	missense	possibly damaging	0.91
R9073:Zfp820	UTSW	17	22,039,031 (GRCm39)	missense	possibly damaging	0.91
R9325:Zfp820	UTSW	17	22,038,380 (GRCm39)	missense	probably damaging	1.00
R9421:Zfp820	UTSW	17	22,038,336 (GRCm39)	missense	probably benign	0.20
R9480:Zfp820	UTSW	17	22,037,994 (GRCm39)	missense	possibly damaging	0.85
R9592:Zfp820	UTSW	17	22,038,577 (GRCm39)	missense	probably benign	0.01
R9600:Zfp820	UTSW	17	22,038,861 (GRCm39)	missense	probably benign	0.00
R9632:Zfp820	UTSW	17	22,038,107 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACAGGGCTTTTAGATTTGCAAGG -3'
(R):5'- ATCCATGATGTGATTGTGAAACAGG -3'

Sequencing Primer
(F):5'- AGGGCTTTTAGATTTGCAAGGTTCTC -3'
(R):5'- TTCAGAGACCCATGGGATG -3'

Posted On

2019-11-26