Mutagenetix > Incidental Mutation

Incidental Mutation 'R7796:Miip'

600219

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R7796 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

147860778-147868816 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 147862918 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 236 (G236S)

Ref Sequence

ENSEMBL: ENSMUSP00000113897 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably benign
Transcript: ENSMUST00000030886
AA Change: G236S

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119975
AA Change: G236S

PolyPhen 2 Score 0.171 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172710
AA Change: G236S

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (54/54)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630089N07Rik	T	A	16: 98,065,824	I313F	probably benign	Het
Acot2	T	A	12: 83,988,483		probably null	Het
Agbl4	G	T	4: 110,660,968	G152V	unknown	Het
Arap2	A	G	5: 62,730,762	S414P	probably damaging	Het
C330021F23Rik	T	C	8: 3,584,160	V111A	probably benign	Het
Cep85l	A	C	10: 53,281,401	N735K	probably damaging	Het
Col12a1	T	C	9: 79,678,551	S1209G	possibly damaging	Het
Dennd1b	G	A	1: 139,062,873	E192K	probably damaging	Het
Dpf1	C	G	7: 29,311,681	N168K	possibly damaging	Het
Dync1li2	T	A	8: 104,430,549	Y184F	probably damaging	Het
Epb41l2	A	G	10: 25,443,829	T187A	probably benign	Het
Fam50b	G	A	13: 34,747,101	E187K	possibly damaging	Het
Fndc3b	G	A	3: 27,461,743	T638I	probably benign	Het
Gm11564	A	G	11: 99,815,598	V2A	unknown	Het
Gm13088	T	A	4: 143,654,157	D432V	possibly damaging	Het
Gm3159	A	G	14: 4,400,560	*206W	probably null	Het
Gm32742	A	G	9: 51,159,823		probably null	Het
Gm4847	G	A	1: 166,642,250	H85Y	probably damaging	Het
Gprc5c	A	T	11: 114,864,532	D345V	probably damaging	Het
Kalrn	A	T	16: 34,187,484	Y1363*	probably null	Het
Kdm5a	T	C	6: 120,390,763	V473A	probably damaging	Het
Kidins220	T	C	12: 24,982,351	C28R	probably damaging	Het
Klhl5	T	C	5: 65,164,622	Y664H	probably damaging	Het
Krt17	A	G	11: 100,260,872	S32P	probably benign	Het
Krt83	A	G	15: 101,485,984	V424A	possibly damaging	Het
Lipo1	T	C	19: 33,782,234	T201A	possibly damaging	Het
Map2	A	G	1: 66,416,495		probably null	Het
Megf10	C	A	18: 57,277,659	T680N	possibly damaging	Het
Metap1d	T	A	2: 71,512,162	D178E	probably damaging	Het
Mki67	A	G	7: 135,698,194	S1704P	probably damaging	Het
Nr2f2	A	G	7: 70,358,153	S194P	probably benign	Het
Nrsn2	T	A	2: 152,376,551		probably benign	Het
Ociad1	C	T	5: 73,294,947	P27L	probably damaging	Het
Olfr1238	T	A	2: 89,406,813	T89S	possibly damaging	Het
Olfr1339	T	A	4: 118,734,685	I52N	probably damaging	Het
Patj	A	T	4: 98,546,983	M1L	probably benign	Het
Pcdhb2	A	G	18: 37,295,393	I140V	possibly damaging	Het
Pla2g6	T	C	15: 79,317,825	N49D	probably benign	Het
Pxdc1	T	A	13: 34,652,371	N22I	probably damaging	Het
Rpgrip1l	A	G	8: 91,270,237	Y672H	probably damaging	Het
Safb2	T	C	17: 56,566,327	R779G	possibly damaging	Het
Skiv2l	C	T	17: 34,844,418	G628S	probably damaging	Het
Smg5	A	T	3: 88,349,432	Q299L	probably damaging	Het
Sptbn2	C	G	19: 4,749,012	R2037G	probably benign	Het
Sulf1	T	C	1: 12,858,820	L864S	probably benign	Het
Tas1r1	A	G	4: 152,034,755	V119A	probably benign	Het
Tasp1	T	C	2: 140,008,785	N106S	probably damaging	Het
Tcf4	T	A	18: 69,564,069	N156K	probably benign	Het
Trav14n-3	T	A	14: 53,370,370	Y52*	probably null	Het
Ttn	T	C	2: 76,834,878	E11598G	unknown	Het
Unc80	A	G	1: 66,503,714	I376V	probably benign	Het
Ybx3	T	C	6: 131,368,516	E298G	probably damaging	Het
Yipf7	T	C	5: 69,527,253	Y73C	possibly damaging	Het
Zdbf2	G	A	1: 63,303,424	A321T	possibly damaging	Het
Zfp369	A	G	13: 65,296,215	R391G	probably benign	Het
Zfp54	A	G	17: 21,434,720	E492G	probably damaging	Het
Zfp971	A	G	2: 178,031,610	T51A	probably benign	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147865865	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147865278	splice site	probably benign
IGL02829:Miip	APN	4	147863061	missense	probably benign	0.01
IGL03350:Miip	APN	4	147862522	missense	probably benign	0.01
R0200:Miip	UTSW	4	147862263	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147865234	missense	probably benign	0.02
R1783:Miip	UTSW	4	147865774	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147863092	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147865965	missense	probably benign	0.15
R3615:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3616:Miip	UTSW	4	147865914	missense	probably benign	0.00
R3882:Miip	UTSW	4	147861052	missense	possibly damaging	0.93
R4579:Miip	UTSW	4	147861061	missense	probably damaging	1.00
R5183:Miip	UTSW	4	147863069	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147865678	missense	probably benign	0.00
R6056:Miip	UTSW	4	147862335	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147863083	missense	probably benign	0.12
R6568:Miip	UTSW	4	147865915	missense	probably benign
R6603:Miip	UTSW	4	147865923	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147862564	missense	probably benign	0.22
R7701:Miip	UTSW	4	147862914	missense	probably null	0.86
R7795:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7797:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7872:Miip	UTSW	4	147862918	missense	probably benign	0.17
R7920:Miip	UTSW	4	147862918	missense	probably benign	0.17
R8468:Miip	UTSW	4	147861471	missense	probably damaging	1.00
R8492:Miip	UTSW	4	147861424	missense	probably damaging	1.00
R8677:Miip	UTSW	4	147863046	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147866382	start gained	probably benign
R8860:Miip	UTSW	4	147866382	start gained	probably benign
R9755:Miip	UTSW	4	147865862	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGGTCAGGGAGAAATGTCCTC -3'
(R):5'- TAGCTCTCCAGTCACTAGTGAG -3'

Sequencing Primer
(F):5'- AGAAATGTCCTCCCAGGGG -3'
(R):5'- AGTCACTAGTGAGCCCGAG -3'

Posted On

2019-11-26