Mutagenetix > Incidental Mutation

Incidental Mutation 'R7797:Miip'

600270

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

045853-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R7797 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

147945235-147953176 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 147947375 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 236 (G236S)

Ref Sequence

ENSEMBL: ENSMUSP00000113897 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably benign
Transcript: ENSMUST00000030886
AA Change: G236S

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119975
AA Change: G236S

PolyPhen 2 Score 0.171 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172710
AA Change: G236S

PolyPhen 2 Score 0.205 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: G236S

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8b	A	G	11: 109,862,509 (GRCm39)		probably null	Het
Adam3	T	G	8: 25,184,660 (GRCm39)	N535T	probably damaging	Het
Adam34l	T	A	8: 44,079,411 (GRCm39)	E271V	probably benign	Het
Adamts4	A	G	1: 171,085,387 (GRCm39)	K679E	probably damaging	Het
Arnt	G	A	3: 95,387,572 (GRCm39)		probably null	Het
Asah2	A	G	19: 31,999,761 (GRCm39)	F321L	probably damaging	Het
Asb15	T	C	6: 24,562,505 (GRCm39)	S156P	probably damaging	Het
Baiap2l1	A	C	5: 144,255,760 (GRCm39)	S65A	probably damaging	Het
Bend7	T	G	2: 4,754,455 (GRCm39)	M186R	probably damaging	Het
Blnk	T	C	19: 40,948,232 (GRCm39)	K146E	possibly damaging	Het
Cd19	A	G	7: 126,012,680 (GRCm39)	W238R	probably damaging	Het
Cd44	T	A	2: 102,679,079 (GRCm39)	N241I	probably benign	Het
Cdh23	T	C	10: 60,220,973 (GRCm39)	E1257G	probably benign	Het
Cfap300	G	T	9: 8,027,130 (GRCm39)	P136Q	possibly damaging	Het
Cntnap5c	T	C	17: 58,666,270 (GRCm39)	V1100A	probably benign	Het
Cul7	T	C	17: 46,969,568 (GRCm39)	V945A	possibly damaging	Het
Dock2	C	T	11: 34,232,652 (GRCm39)	C1116Y	probably damaging	Het
Ehbp1	T	C	11: 22,046,109 (GRCm39)	M547V	possibly damaging	Het
Fam50b	G	A	13: 34,931,084 (GRCm39)	E187K	possibly damaging	Het
Grhl3	C	G	4: 135,286,416 (GRCm39)	K88N	possibly damaging	Het
Hbb-y	T	A	7: 103,501,088 (GRCm39)	Y146F	probably damaging	Het
Hivep2	T	C	10: 14,005,847 (GRCm39)	V815A	probably benign	Het
Hoxa7	T	A	6: 52,192,870 (GRCm39)	I173F	probably damaging	Het
Igkv1-110	T	G	6: 68,247,977 (GRCm39)	S29A	probably benign	Het
Jade2	A	G	11: 51,708,126 (GRCm39)	S696P	probably benign	Het
Kif1b	T	A	4: 149,321,844 (GRCm39)	Q1025L	probably benign	Het
Kif2c	A	T	4: 117,028,940 (GRCm39)	C241S	probably benign	Het
Kmt2d	G	T	15: 98,762,287 (GRCm39)	H400N	probably benign	Het
Krt35	T	A	11: 99,985,713 (GRCm39)	N174Y	probably damaging	Het
Ldlrad1	G	A	4: 107,066,688 (GRCm39)	A8T	probably benign	Het
Magi3	A	G	3: 103,958,618 (GRCm39)	V489A	probably damaging	Het
Mapk7	T	C	11: 61,380,241 (GRCm39)	E739G	possibly damaging	Het
Mcidas	G	A	13: 113,135,521 (GRCm39)	G315S	probably damaging	Het
Mdga1	C	T	17: 30,061,814 (GRCm39)		probably null	Het
Megf8	G	A	7: 25,034,022 (GRCm39)	R607H	probably damaging	Het
Met	T	A	6: 17,533,952 (GRCm39)	N634K	probably damaging	Het
Mmp11	G	T	10: 75,759,314 (GRCm39)	T107K		Het
Mroh2b	A	C	15: 4,978,587 (GRCm39)	N1378H	probably benign	Het
Naa15	G	A	3: 51,356,031 (GRCm39)	C322Y	probably damaging	Het
Naip1	T	A	13: 100,580,986 (GRCm39)	Y87F	probably damaging	Het
Ngrn	A	G	7: 79,914,185 (GRCm39)	D112G	probably benign	Het
Osbpl1a	C	A	18: 13,015,321 (GRCm39)	C369F	probably damaging	Het
Pcnx2	G	C	8: 126,512,087 (GRCm39)	D1406E	possibly damaging	Het
Phf8-ps	T	C	17: 33,286,664 (GRCm39)	D46G	probably damaging	Het
Pold1	G	A	7: 44,191,213 (GRCm39)	P206L	probably benign	Het
Psen1	A	G	12: 83,746,396 (GRCm39)	S20G	probably benign	Het
Ptpn23	C	T	9: 110,222,875 (GRCm39)	D61N	possibly damaging	Het
Rbfox3	A	T	11: 118,387,310 (GRCm39)	L268Q	possibly damaging	Het
Rbm22	A	G	18: 60,694,344 (GRCm39)	T26A	probably damaging	Het
Rpgrip1	G	A	14: 52,371,277 (GRCm39)	R332Q	possibly damaging	Het
Rrm2b	A	T	15: 37,927,505 (GRCm39)	L347*	probably null	Het
Rsf1	A	T	7: 97,310,692 (GRCm39)	D474V		Het
Ryr2	C	T	13: 11,816,066 (GRCm39)	R640Q	probably damaging	Het
Sec16b	A	G	1: 157,389,245 (GRCm39)	E691G	unknown	Het
Septin1	A	T	7: 126,813,937 (GRCm39)	V365E	unknown	Het
Tlk2	A	G	11: 105,101,444 (GRCm39)	H114R	probably benign	Het
Tmem200a	A	G	10: 25,869,864 (GRCm39)	I135T	possibly damaging	Het
Tpst2	T	A	5: 112,455,782 (GRCm39)	V107E	probably damaging	Het
Trim30a	A	T	7: 104,060,407 (GRCm39)	D456E	possibly damaging	Het
Ttll3	T	C	6: 113,371,738 (GRCm39)	V45A	possibly damaging	Het
Ulk2	A	G	11: 61,672,928 (GRCm39)	Y889H	probably benign	Het
Umodl1	A	G	17: 31,178,125 (GRCm39)	S34G	probably benign	Het
Vmn1r67	A	G	7: 10,180,903 (GRCm39)	I56V	probably benign	Het
Vmn2r120	C	T	17: 57,815,874 (GRCm39)	G827E	probably damaging	Het
Zc3h6	T	C	2: 128,857,555 (GRCm39)		probably null	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147,950,322 (GRCm39)	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147,949,735 (GRCm39)	splice site	probably benign
IGL02829:Miip	APN	4	147,947,518 (GRCm39)	missense	probably benign	0.01
IGL03350:Miip	APN	4	147,946,979 (GRCm39)	missense	probably benign	0.01
R0200:Miip	UTSW	4	147,946,720 (GRCm39)	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147,949,691 (GRCm39)	missense	probably benign	0.02
R1783:Miip	UTSW	4	147,950,231 (GRCm39)	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147,947,549 (GRCm39)	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147,950,422 (GRCm39)	missense	probably benign	0.15
R3615:Miip	UTSW	4	147,950,371 (GRCm39)	missense	probably benign	0.00
R3616:Miip	UTSW	4	147,950,371 (GRCm39)	missense	probably benign	0.00
R3882:Miip	UTSW	4	147,945,509 (GRCm39)	missense	possibly damaging	0.93
R4579:Miip	UTSW	4	147,945,518 (GRCm39)	missense	probably damaging	1.00
R5183:Miip	UTSW	4	147,947,526 (GRCm39)	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147,950,135 (GRCm39)	missense	probably benign	0.00
R6056:Miip	UTSW	4	147,946,792 (GRCm39)	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147,947,540 (GRCm39)	missense	probably benign	0.12
R6568:Miip	UTSW	4	147,950,372 (GRCm39)	missense	probably benign
R6603:Miip	UTSW	4	147,950,380 (GRCm39)	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147,947,021 (GRCm39)	missense	probably benign	0.22
R7701:Miip	UTSW	4	147,947,371 (GRCm39)	missense	probably null	0.86
R7795:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7796:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7872:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7920:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R8468:Miip	UTSW	4	147,945,928 (GRCm39)	missense	probably damaging	1.00
R8492:Miip	UTSW	4	147,945,881 (GRCm39)	missense	probably damaging	1.00
R8677:Miip	UTSW	4	147,947,503 (GRCm39)	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147,950,839 (GRCm39)	start gained	probably benign
R8860:Miip	UTSW	4	147,950,839 (GRCm39)	start gained	probably benign
R9755:Miip	UTSW	4	147,950,319 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGGGAGAAATGTCCTCCCAG -3'
(R):5'- GGACAATAGCTCTCCAGTCACTAG -3'

Sequencing Primer
(F):5'- AAATGTCCTCCCAGGGGCAG -3'
(R):5'- ATAGCTCTCCAGTCACTAGTGAGC -3'

Posted On

2019-11-26