Mutagenetix > Incidental Mutation

Incidental Mutation 'R7808:Vmn2r28'

600932

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

045863-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R7808 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

5483455-5496850 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 5496678 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 58 (Y58C)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably damaging
Transcript: ENSMUST00000086297
AA Change: Y58C

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: Y58C

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

99% (86/87)

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	T	C	10: 86,957,297 (GRCm39)	V11A	probably benign	Het
Abca12	A	T	1: 71,313,793 (GRCm39)		probably null	Het
Abcc12	T	A	8: 87,234,568 (GRCm39)	M1232L	probably benign	Het
Adamts1	T	C	16: 85,597,117 (GRCm39)	Y314C	probably damaging	Het
Adgb	A	G	10: 10,254,403 (GRCm39)		probably null	Het
Ankrd12	T	A	17: 66,292,648 (GRCm39)	K928N	possibly damaging	Het
Ano5	A	T	7: 51,237,543 (GRCm39)	K789I	possibly damaging	Het
Cacna1d	G	T	14: 29,833,026 (GRCm39)	N938K	probably damaging	Het
Camk1g	T	C	1: 193,032,593 (GRCm39)	R273G	possibly damaging	Het
Caprin2	C	A	6: 148,744,528 (GRCm39)	V966F	probably damaging	Het
Card6	T	C	15: 5,128,954 (GRCm39)	H814R	probably benign	Het
Cenatac	T	C	9: 44,324,215 (GRCm39)	Q228R	probably null	Het
Cflar	A	G	1: 58,750,740 (GRCm39)		probably benign	Het
Cftr	T	A	6: 18,204,204 (GRCm39)	N66K	probably benign	Het
Clasrp	C	T	7: 19,322,671 (GRCm39)		probably null	Het
Clec4a4	T	C	6: 122,967,339 (GRCm39)	I5T	probably damaging	Het
Cmtr2	T	C	8: 110,948,251 (GRCm39)	I187T	possibly damaging	Het
Cnga1	A	T	5: 72,761,616 (GRCm39)	F633I	possibly damaging	Het
Col16a1	C	T	4: 129,967,057 (GRCm39)	P909S	unknown	Het
Csf1	C	A	3: 107,667,361 (GRCm39)	A7S	possibly damaging	Het
Cstpp1	T	C	2: 91,274,939 (GRCm39)		probably benign	Het
Dhx30	A	T	9: 109,915,270 (GRCm39)	V833D	probably benign	Het
Dlg2	T	G	7: 92,080,263 (GRCm39)	I712M	probably benign	Het
Dmxl1	T	A	18: 50,011,382 (GRCm39)	F1180I	probably benign	Het
Dnah9	T	A	11: 65,896,631 (GRCm39)	K2398*	probably null	Het
Dus1l	C	T	11: 120,680,262 (GRCm39)	G471D	possibly damaging	Het
Dync1h1	A	T	12: 110,621,893 (GRCm39)	N3412I	possibly damaging	Het
Dync1i2	T	A	2: 71,081,178 (GRCm39)		probably null	Het
Dysf	T	C	6: 84,047,911 (GRCm39)	S333P	possibly damaging	Het
Eed	A	T	7: 89,605,541 (GRCm39)	N349K	probably benign	Het
Eipr1	A	T	12: 28,816,769 (GRCm39)		probably null	Het
Fbxw16	A	C	9: 109,277,222 (GRCm39)	V40G	probably damaging	Het
Fgfr4	A	C	13: 55,308,969 (GRCm39)	R363S	possibly damaging	Het
Fgl2	A	T	5: 21,578,229 (GRCm39)	N172I	possibly damaging	Het
Fhip1b	G	A	7: 105,033,732 (GRCm39)	R509C	probably damaging	Het
Gabbr2	T	A	4: 46,875,744 (GRCm39)	H126L	possibly damaging	Het
Gcnt2	A	T	13: 41,014,338 (GRCm39)	N170Y	possibly damaging	Het
Gpt	A	G	15: 76,583,093 (GRCm39)		probably null	Het
Igsf10	A	G	3: 59,235,489 (GRCm39)	I1564T	probably benign	Het
Il22ra1	C	A	4: 135,478,107 (GRCm39)	Q393K	possibly damaging	Het
Inpp5d	A	T	1: 87,611,567 (GRCm39)	K340*	probably null	Het
Jcad	A	G	18: 4,673,113 (GRCm39)	K292E	probably damaging	Het
Krt5	T	A	15: 101,617,453 (GRCm39)	T427S	probably benign	Het
Krt76	T	C	15: 101,798,929 (GRCm39)	D252G	probably damaging	Het
Krtap26-1	A	G	16: 88,444,198 (GRCm39)	V141A	not run	Het
Lce6a	A	T	3: 92,527,642 (GRCm39)	V55D	probably benign	Het
Lpxn	C	T	19: 12,802,185 (GRCm39)	S170F	possibly damaging	Het
Magi2	T	C	5: 20,670,838 (GRCm39)	V394A	probably benign	Het
Mbnl1	G	A	3: 60,522,242 (GRCm39)		probably null	Het
Med16	T	A	10: 79,734,252 (GRCm39)	K554M	probably damaging	Het
Mettl14	T	C	3: 123,166,234 (GRCm39)	D276G	possibly damaging	Het
Mphosph9	T	C	5: 124,399,009 (GRCm39)	D1002G	probably damaging	Het
Mroh9	T	C	1: 162,866,678 (GRCm39)	E686G	probably damaging	Het
Nav1	T	C	1: 135,379,986 (GRCm39)	Y1512C	unknown	Het
Neb	T	C	2: 52,082,035 (GRCm39)	Y5712C	probably damaging	Het
Nek7	T	A	1: 138,489,509 (GRCm39)		probably benign	Het
Nptx1	T	A	11: 119,435,462 (GRCm39)	I285F	probably damaging	Het
Oas1d	G	T	5: 121,053,034 (GRCm39)	E30*	probably null	Het
Or1p1b	A	T	11: 74,130,725 (GRCm39)	I112F	probably damaging	Het
Or51g2	A	G	7: 102,622,317 (GRCm39)	V294A	possibly damaging	Het
Or6n1	C	A	1: 173,917,417 (GRCm39)	Y270*	probably null	Het
Parp4	T	G	14: 56,873,205 (GRCm39)	S1150A	possibly damaging	Het
Pinx1	A	T	14: 64,156,741 (GRCm39)	K223*	probably null	Het
Plekha5	T	C	6: 140,529,640 (GRCm39)	L1034S	probably damaging	Het
Ppp4r3a	A	G	12: 101,019,755 (GRCm39)	V400A	possibly damaging	Het
Prtg	A	T	9: 72,749,979 (GRCm39)	I128F	possibly damaging	Het
Rars1	T	C	11: 35,719,534 (GRCm39)	E96G	probably benign	Het
Rhpn1	T	A	15: 75,585,299 (GRCm39)	S551T	probably benign	Het
Selenow	C	T	7: 15,656,176 (GRCm39)		probably null	Het
Serpina9	A	T	12: 103,967,484 (GRCm39)		probably null	Het
Shank3	A	T	15: 89,433,083 (GRCm39)	D1276V	probably damaging	Het
Slc27a6	A	G	18: 58,742,267 (GRCm39)	T494A	probably damaging	Het
Slc34a1	A	C	13: 24,003,317 (GRCm39)	H285P	probably damaging	Het
Slc6a21	A	C	7: 44,932,360 (GRCm39)	T54P		Het
Syne2	A	G	12: 76,030,501 (GRCm39)		probably null	Het
Tal1	T	C	4: 114,925,489 (GRCm39)	V186A	probably benign	Het
Tars3	A	G	7: 65,302,009 (GRCm39)	K178E	probably benign	Het
Tex10	T	A	4: 48,459,984 (GRCm39)	I456L	probably benign	Het
Tfcp2	A	G	15: 100,420,310 (GRCm39)	F175S	probably damaging	Het
Timp2	C	T	11: 118,194,626 (GRCm39)	A188T	probably damaging	Het
Tmc5	T	A	7: 118,268,440 (GRCm39)	I836N	probably damaging	Het
Tmem100	T	A	11: 89,926,302 (GRCm39)	M43K	probably benign	Het
Ulk2	A	G	11: 61,745,378 (GRCm39)	Y9H	probably damaging	Het
Usp7	T	C	16: 8,523,027 (GRCm39)	K311E	probably damaging	Het
Vmn1r238	G	A	18: 3,123,033 (GRCm39)	T127I	probably benign	Het
Wdr55	G	A	18: 36,893,469 (GRCm39)	G44S	probably benign	Het
Zfp677	C	A	17: 21,617,647 (GRCm39)	H235N	probably damaging	Het
Zfp869	T	A	8: 70,159,636 (GRCm39)	R312S	probably damaging	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5,491,068 (GRCm39)	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5,491,183 (GRCm39)	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5,489,477 (GRCm39)	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5,484,087 (GRCm39)	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5,489,463 (GRCm39)	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5,491,161 (GRCm39)	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5,484,302 (GRCm39)	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5,491,123 (GRCm39)	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5,493,568 (GRCm39)	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5,484,003 (GRCm39)	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5,487,015 (GRCm39)	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5,491,020 (GRCm39)	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5,496,715 (GRCm39)	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5,491,513 (GRCm39)	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5,493,456 (GRCm39)	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5,491,689 (GRCm39)	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R1282:Vmn2r28	UTSW	7	5,484,301 (GRCm39)	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5,484,544 (GRCm39)	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5,496,810 (GRCm39)	missense	probably benign
R1853:Vmn2r28	UTSW	7	5,484,246 (GRCm39)	nonsense	probably null
R1869:Vmn2r28	UTSW	7	5,489,345 (GRCm39)	missense	probably benign	0.00
R1887:Vmn2r28	UTSW	7	5,491,288 (GRCm39)	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5,484,070 (GRCm39)	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5,491,313 (GRCm39)	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5,487,130 (GRCm39)	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5,491,701 (GRCm39)	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5,484,391 (GRCm39)	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5,491,357 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5,493,707 (GRCm39)	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5,483,671 (GRCm39)	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5,489,376 (GRCm39)	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5,490,943 (GRCm39)	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5,487,011 (GRCm39)	missense	possibly damaging	0.61
R5731:Vmn2r28	UTSW	7	5,491,668 (GRCm39)	missense	probably benign	0.41
R6091:Vmn2r28	UTSW	7	5,496,790 (GRCm39)	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5,491,003 (GRCm39)	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5,493,730 (GRCm39)	missense	probably benign	0.01
R6441:Vmn2r28	UTSW	7	5,491,474 (GRCm39)	missense	probably benign	0.00
R6463:Vmn2r28	UTSW	7	5,489,435 (GRCm39)	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5,493,684 (GRCm39)	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5,484,229 (GRCm39)	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5,483,806 (GRCm39)	missense	probably damaging	1.00
R7110:Vmn2r28	UTSW	7	5,493,733 (GRCm39)	missense	probably benign	0.06
R7146:Vmn2r28	UTSW	7	5,484,495 (GRCm39)	missense	probably benign	0.05
R7407:Vmn2r28	UTSW	7	5,484,308 (GRCm39)	missense	probably damaging	1.00
R7563:Vmn2r28	UTSW	7	5,491,200 (GRCm39)	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5,484,255 (GRCm39)	missense	probably benign	0.02
R7862:Vmn2r28	UTSW	7	5,493,613 (GRCm39)	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5,483,818 (GRCm39)	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5,491,147 (GRCm39)	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5,487,059 (GRCm39)	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5,489,347 (GRCm39)	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5,493,473 (GRCm39)	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5,491,596 (GRCm39)	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5,483,747 (GRCm39)	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5,487,118 (GRCm39)	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5,491,220 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- GGAGGCCACCTGACTTAAAG -3'
(R):5'- ACACTGACCAGGTTTAAGGCAG -3'

Sequencing Primer
(F):5'- CCTCCACTGAATTACTTAAAAGGAG -3'
(R):5'- GGTTTAAGGCAGGACAAATATGTTC -3'

Posted On

2019-11-26