Mutagenetix > Incidental Mutation

Incidental Mutation 'R7811:Or13g1'

601166

Institutional Source

Beutler Lab

Gene Symbol

Or13g1

Ensembl Gene

ENSMUSG00000054054

Gene Name

olfactory receptor family 13 subfamily G member 1

Synonyms

MOR251-4P, GA_x6K02T2NHDJ-9801340-9802266, Olfr309

MMRRC Submission

045866-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R7811 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85955393-85956319 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85955554 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 256 (T256S)

Ref Sequence

ENSEMBL: ENSMUSP00000134377 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055690] [ENSMUST00000174158]

AlphaFold

Q7TS01

Predicted Effect

probably damaging
Transcript: ENSMUST00000055690
AA Change: T256S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000061929
Gene: ENSMUSG00000054054
AA Change: T256S

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	303	4.5e-11	PFAM
Pfam:7tm_1	39	288	1.5e-26	PFAM
Pfam:7tm_4	137	281	7.9e-44	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000174158
AA Change: T256S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000134377
Gene: ENSMUSG00000054054
AA Change: T256S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	305	1.6e-55	PFAM
Pfam:7TM_GPCR_Srsx	33	303	4.5e-11	PFAM
Pfam:7tm_1	39	288	2.4e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (58/58)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,527,141 (GRCm39)		probably null	Het
Abca3	A	G	17: 24,616,362 (GRCm39)	T935A	probably benign	Het
Anxa7	A	T	14: 20,510,254 (GRCm39)	Y399N	probably benign	Het
Arhgef10l	G	T	4: 140,242,335 (GRCm39)	T1030K	possibly damaging	Het
Ass1	G	A	2: 31,404,753 (GRCm39)	V345I	probably benign	Het
Bms1	T	C	6: 118,380,099 (GRCm39)	D736G	probably damaging	Het
Bpifb1	T	A	2: 154,048,484 (GRCm39)		probably null	Het
Cbx4	A	C	11: 118,972,398 (GRCm39)	C326G	probably benign	Het
Ccdc188	A	G	16: 18,036,314 (GRCm39)	E94G	probably benign	Het
Cdh5	A	G	8: 104,852,235 (GRCm39)	T117A	possibly damaging	Het
Cdk11b	A	G	4: 155,724,359 (GRCm39)	T326A	unknown	Het
Clec5a	C	T	6: 40,558,867 (GRCm39)	C73Y	probably damaging	Het
Cntrl	T	C	2: 35,052,873 (GRCm39)	M1126T	probably benign	Het
Diaph3	T	C	14: 87,219,060 (GRCm39)	D455G	probably damaging	Het
Eml2	T	A	7: 18,920,047 (GRCm39)	M117K	probably benign	Het
Ern1	A	T	11: 106,325,694 (GRCm39)	V112D	unknown	Het
Eya3	T	C	4: 132,439,272 (GRCm39)	I466T	possibly damaging	Het
Fbxl6	T	C	15: 76,421,485 (GRCm39)		probably null	Het
Ffar1	A	G	7: 30,560,802 (GRCm39)	S32P	possibly damaging	Het
Fgb	T	C	3: 82,957,004 (GRCm39)	D22G	probably benign	Het
Fsip2	T	C	2: 82,828,797 (GRCm39)	Y6865H	possibly damaging	Het
Gbx1	T	C	5: 24,710,035 (GRCm39)	E270G	probably damaging	Het
Golga4	T	C	9: 118,361,643 (GRCm39)	L207P	probably damaging	Het
Hnrnpa1	T	C	15: 103,149,900 (GRCm39)	S54P	possibly damaging	Het
Htr4	A	G	18: 62,545,269 (GRCm39)	E18G	possibly damaging	Het
Ide	A	C	19: 37,307,910 (GRCm39)	L34R		Het
Ip6k3	A	T	17: 27,376,557 (GRCm39)	Y51*	probably null	Het
Keap1	A	G	9: 21,148,956 (GRCm39)	L17P	possibly damaging	Het
Lrrn2	T	C	1: 132,866,939 (GRCm39)	V668A	probably benign	Het
Lypd8	A	T	11: 58,281,064 (GRCm39)	T209S	possibly damaging	Het
Myo10	T	C	15: 25,804,610 (GRCm39)	I1635T	probably damaging	Het
Myo1e	T	A	9: 70,234,544 (GRCm39)	V299E	probably damaging	Het
Nadk	A	T	4: 155,661,332 (GRCm39)		probably benign	Het
Nalcn	A	T	14: 123,536,357 (GRCm39)	W1231R	probably damaging	Het
Odf2l	A	G	3: 144,859,148 (GRCm39)	T602A	probably benign	Het
Or2r3	T	A	6: 42,448,635 (GRCm39)	E159V	probably damaging	Het
Pde6c	T	C	19: 38,128,507 (GRCm39)	V190A	possibly damaging	Het
Ppp4r3a	C	A	12: 101,019,821 (GRCm39)	R378L	probably damaging	Het
Pus7l	A	C	15: 94,438,707 (GRCm39)	V46G	probably damaging	Het
Satb2	G	A	1: 56,884,880 (GRCm39)	S466L	probably benign	Het
Serpina6	A	G	12: 103,620,395 (GRCm39)	M118T	probably damaging	Het
Slc29a1	T	C	17: 45,897,189 (GRCm39)	R366G	probably damaging	Het
Slc6a1	T	C	6: 114,279,515 (GRCm39)	F98S	probably damaging	Het
Spg7	T	C	8: 123,824,164 (GRCm39)	I738T	possibly damaging	Het
Srcap	T	C	7: 127,141,221 (GRCm39)	V1667A	probably damaging	Het
Stxbp5	T	C	10: 9,684,248 (GRCm39)	N574S	probably benign	Het
Syne2	A	G	12: 76,030,501 (GRCm39)		probably null	Het
Tbc1d10a	T	C	11: 4,136,948 (GRCm39)	S54P	possibly damaging	Het
Trim12c	T	C	7: 103,990,469 (GRCm39)	N336S	unknown	Het
Ubap2	T	C	4: 41,211,710 (GRCm39)	S351G	probably benign	Het
Unc13c	G	T	9: 73,600,553 (GRCm39)	A1397E	possibly damaging	Het
Ush1c	G	A	7: 45,854,710 (GRCm39)	R681*	probably null	Het
Vmn2r57	A	T	7: 41,074,439 (GRCm39)	C541*	probably null	Het
Vmn2r73	T	C	7: 85,524,956 (GRCm39)	D64G	possibly damaging	Het
Vmn2r88	A	T	14: 51,656,160 (GRCm39)	T790S		Het
Zfp568	A	G	7: 29,697,295 (GRCm39)	T72A	possibly damaging	Het
Zfp950	A	T	19: 61,108,353 (GRCm39)	Y243*	probably null	Het
Zmym5	A	G	14: 57,036,434 (GRCm39)	S238P	probably damaging	Het
Zswim5	A	G	4: 116,734,673 (GRCm39)	E6G	unknown	Het

Other mutations in Or13g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00886:Or13g1	APN	7	85,956,259 (GRCm39)	missense	probably damaging	1.00
R0370:Or13g1	UTSW	7	85,956,057 (GRCm39)	missense	probably benign	0.42
R1869:Or13g1	UTSW	7	85,955,875 (GRCm39)	missense	possibly damaging	0.96
R2047:Or13g1	UTSW	7	85,956,012 (GRCm39)	missense	probably damaging	1.00
R3939:Or13g1	UTSW	7	85,955,437 (GRCm39)	missense	probably benign	0.00
R4562:Or13g1	UTSW	7	85,956,360 (GRCm39)	start gained	probably benign
R4640:Or13g1	UTSW	7	85,956,274 (GRCm39)	missense	probably benign	0.01
R4811:Or13g1	UTSW	7	85,956,166 (GRCm39)	missense	probably benign	0.41
R4867:Or13g1	UTSW	7	85,955,491 (GRCm39)	missense	probably benign	0.00
R4954:Or13g1	UTSW	7	85,955,809 (GRCm39)	missense	probably benign	0.01
R5784:Or13g1	UTSW	7	85,955,743 (GRCm39)	missense	probably damaging	1.00
R6043:Or13g1	UTSW	7	85,955,547 (GRCm39)	missense	probably damaging	0.97
R6498:Or13g1	UTSW	7	85,956,226 (GRCm39)	missense	probably benign
R6849:Or13g1	UTSW	7	85,956,248 (GRCm39)	missense	possibly damaging	0.54
R7238:Or13g1	UTSW	7	85,955,799 (GRCm39)	missense	probably damaging	1.00
R7917:Or13g1	UTSW	7	85,955,686 (GRCm39)	missense	probably damaging	1.00
R8010:Or13g1	UTSW	7	85,956,260 (GRCm39)	missense	probably benign	0.24
R8271:Or13g1	UTSW	7	85,955,962 (GRCm39)	missense	probably benign	0.13
R9017:Or13g1	UTSW	7	85,955,957 (GRCm39)	nonsense	probably null
R9150:Or13g1	UTSW	7	85,955,942 (GRCm39)	missense	probably damaging	0.96
T0975:Or13g1	UTSW	7	85,955,492 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTGGAGTAAGGTTGTATTCCATTC -3'
(R):5'- TAGTCCTGTAAGAGTCAATGAGGTG -3'

Sequencing Primer
(F):5'- GTAAGGTTGTATTCCATTCTGAAACC -3'
(R):5'- GATGGTGTATGTTGCTGATATCACCC -3'

Posted On

2019-11-26