Mutagenetix > Incidental Mutations

Incidental Mutation 'R7812:Psg25'

601217

Institutional Source

Beutler Lab

Gene Symbol

Psg25

Ensembl Gene

ENSMUSG00000070798

Gene Name

pregnancy-specific glycoprotein 25

Synonyms

cea13

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R7812 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

18519702-18532269 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 18521168 bp

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 474 (N474K)

Ref Sequence

ENSEMBL: ENSMUSP00000092389 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094795]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094795
AA Change: N474K

PolyPhen 2 Score 0.712 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000092389
Gene: ENSMUSG00000070798
AA Change: N474K

Domain	Start	End	E-Value	Type
IG	40	141	2.15e-3	SMART
IG	160	261	1.55e0	SMART
IG	280	381	3.59e-5	SMART
IGc2	397	461	1.02e-9	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	C	A	9: 57,257,858	C411F	probably damaging	Het
Abhd17c	G	T	7: 84,151,416	Y143*	probably null	Het
Acad11	T	G	9: 104,095,548	L480R	probably benign	Het
Acad8	A	T	9: 26,979,180	N277K	probably damaging	Het
Adcy10	A	G	1: 165,515,369	Y268C	probably damaging	Het
Adm2	A	T	15: 89,324,164	R102S	possibly damaging	Het
Ambra1	A	T	2: 91,766,566	M1L	probably benign	Het
Angpt2	T	C	8: 18,692,145	N465S	probably benign	Het
Arhgap17	T	C	7: 123,280,067	I820M	unknown	Het
Atf7ip	G	A	6: 136,603,417	R1113Q	probably damaging	Het
Atp5f1	A	G	3: 105,943,841	V180A	probably benign	Het
Auts2	C	T	5: 131,472,446	R43H		Het
Cacna1g	A	T	11: 94,444,054	I809N	probably benign	Het
Cand1	A	T	10: 119,217,959	M158K	probably benign	Het
Cars	T	A	7: 143,570,047	I500F	probably damaging	Het
Ccdc18	T	A	5: 108,180,833	M706K	probably benign	Het
Ccdc180	G	A	4: 45,906,952		probably null	Het
Ces1f	A	G	8: 93,258,310	V463A	probably benign	Het
Cndp1	G	T	18: 84,637,869	D58E	probably benign	Het
Csnk2b	A	T	17: 35,120,455	F21Y	probably benign	Het
Dgkg	C	G	16: 22,566,415	R447T	probably damaging	Het
Dnajc12	A	G	10: 63,407,126	D141G	probably benign	Het
Edar	A	G	10: 58,630,104	V4A	probably benign	Het
Elf1	A	G	14: 79,565,558	D117G	probably damaging	Het
Fam124b	T	C	1: 80,213,634	T11A	probably damaging	Het
Fgl2	C	A	5: 21,372,898	T61K	probably benign	Het
Furin	T	C	7: 80,395,974	N218S	possibly damaging	Het
Gcn1l1	T	A	5: 115,593,692	H955Q	possibly damaging	Het
Gucy2e	A	G	11: 69,226,243	I784T	probably damaging	Het
Ice1	T	A	13: 70,603,005	Q1654L	possibly damaging	Het
Klhl28	T	C	12: 64,943,589	N526S	possibly damaging	Het
Ldlrad4	C	A	18: 68,106,671	P54Q	probably benign	Het
Lsm14a	A	G	7: 34,388,876		probably benign	Het
Mdga1	A	G	17: 29,843,141	V438A	probably benign	Het
Mrto4	A	G	4: 139,347,967	V175A	possibly damaging	Het
Nav2	T	G	7: 49,597,173	S2149A	probably benign	Het
Nr4a2	A	G	2: 57,112,418	Y8H	probably damaging	Het
Olfr1495	T	C	19: 13,769,016	F225L	probably benign	Het
Olfr340	T	A	2: 36,453,278	I231N	probably benign	Het
Olfr347	A	T	2: 36,734,725	M135L	probably benign	Het
Olfr677	T	C	7: 105,056,687	L147P	possibly damaging	Het
Oxr1	T	A	15: 41,751,742	M1K	probably null	Het
Pbx3	T	C	2: 34,224,466	Y152C	probably damaging	Het
Pcdhb13	T	A	18: 37,442,592	C8S	probably benign	Het
Polr1c	G	A	17: 46,246,127	R77W	probably damaging	Het
Ralgapa1	T	A	12: 55,719,628	M838L	possibly damaging	Het
Ranbp2	A	G	10: 58,467,402	T799A	probably benign	Het
Sbf2	C	T	7: 110,449,963	V265I	possibly damaging	Het
Scarf2	G	A	16: 17,803,828	G381R	probably damaging	Het
Shprh	A	G	10: 11,151,991	Q114R	probably benign	Het
Sipa1l2	A	C	8: 125,491,595	D334E	probably damaging	Het
Slc26a4	T	A	12: 31,544,450	I300F	probably damaging	Het
Sox7	C	T	14: 63,948,232	T239I	probably benign	Het
Sry	GCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTG	GCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTG	Y: 2,662,638		probably benign	Het
St6galnac1	G	T	11: 116,769,101	H129N	probably benign	Het
Stxbp4	A	G	11: 90,594,828	L231P	probably damaging	Het
Syngap1	A	C	17: 26,941,504	Y18S	probably benign	Het
Tenm3	T	C	8: 48,276,300	Y1557C	probably benign	Het
Trim44	G	T	2: 102,400,144	P181T	possibly damaging	Het
Trp53i13	A	T	11: 77,508,840	V221D	possibly damaging	Het
Trpm7	A	G	2: 126,799,316	S1554P	probably damaging	Het
Vcl	A	G	14: 20,995,090	N288D	probably benign	Het
Vmn2r59	A	T	7: 42,045,772	Y405*	probably null	Het
Vps35	T	C	8: 85,284,189	D175G	probably benign	Het
Wdr90	A	T	17: 25,852,558	L949Q	probably damaging	Het
Wwp1	G	A	4: 19,639,991	T517I	probably damaging	Het
Zfp462	T	G	4: 55,008,509	F158L	probably benign	Het
Zfp703	T	A	8: 26,979,878	H523Q	probably damaging	Het

Other mutations in Psg25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Psg25	APN	7	18526181	splice site	probably benign
IGL00508:Psg25	APN	7	18529731	missense	probably benign
IGL01154:Psg25	APN	7	18524699	missense	probably benign	0.01
IGL01388:Psg25	APN	7	18529665	missense	possibly damaging	0.87
IGL02222:Psg25	APN	7	18529727	missense	probably damaging	1.00
IGL02261:Psg25	APN	7	18521343	missense	probably benign	0.09
IGL02309:Psg25	APN	7	18526424	missense	probably damaging	0.98
IGL02803:Psg25	APN	7	18526287	missense	possibly damaging	0.47
IGL03334:Psg25	APN	7	18529774	missense	probably benign	0.01
R0711:Psg25	UTSW	7	18529560	nonsense	probably null
R1458:Psg25	UTSW	7	18529587	missense	probably damaging	1.00
R1598:Psg25	UTSW	7	18532003	nonsense	probably null
R2064:Psg25	UTSW	7	18521253	missense	probably damaging	0.96
R2066:Psg25	UTSW	7	18529562	missense	probably damaging	1.00
R4485:Psg25	UTSW	7	18526278	missense	probably damaging	1.00
R4499:Psg25	UTSW	7	18524891	missense	possibly damaging	0.89
R4514:Psg25	UTSW	7	18529608	nonsense	probably null
R4547:Psg25	UTSW	7	18524704	missense	probably damaging	1.00
R4604:Psg25	UTSW	7	18529803	missense	probably benign	0.05
R4886:Psg25	UTSW	7	18524913	missense	probably benign	0.00
R5121:Psg25	UTSW	7	18526536	missense	possibly damaging	0.68
R5208:Psg25	UTSW	7	18526535	missense	probably benign	0.00
R5267:Psg25	UTSW	7	18524786	missense	possibly damaging	0.78
R5376:Psg25	UTSW	7	18526535	missense	probably benign	0.00
R5425:Psg25	UTSW	7	18524784	nonsense	probably null
R5749:Psg25	UTSW	7	18524851	missense	probably damaging	1.00
R6050:Psg25	UTSW	7	18526478	missense	probably benign	0.37
R6862:Psg25	UTSW	7	18521398	missense	probably benign	0.03
R6962:Psg25	UTSW	7	18529754	missense	probably damaging	1.00
R7238:Psg25	UTSW	7	18532202	start gained	probably benign
R7782:Psg25	UTSW	7	18521302	missense	probably benign	0.15
Z1088:Psg25	UTSW	7	18529591	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCACTGTGCAACAATAGTAAGC -3'
(R):5'- CCGTTGGCTCTTCAACAATCAG -3'

Sequencing Primer
(F):5'- CCAAGGGAGTATGCTTGAATCTCAC -3'
(R):5'- TGGCTCTTCAACAATCAGAATCTGC -3'

Posted On

2019-11-26