Mutagenetix > Incidental Mutation

Incidental Mutation 'R7814:Map10'

601369

Institutional Source

Beutler Lab

Gene Symbol

Map10

Ensembl Gene

ENSMUSG00000050930

Gene Name

microtubule-associated protein 10

Synonyms

4933403G14Rik

MMRRC Submission

045869-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R7814 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

125669818-125673359 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 125671611 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 581 (E581G)

Ref Sequence

ENSEMBL: ENSMUSP00000061679 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053078]

AlphaFold

Q8BJS7

Predicted Effect

probably benign
Transcript: ENSMUST00000053078
AA Change: E581G

PolyPhen 2 Score 0.137 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000061679
Gene: ENSMUSG00000050930
AA Change: E581G

Domain	Start	End	E-Value	Type
low complexity region	38	54	N/A	INTRINSIC
low complexity region	68	91	N/A	INTRINSIC
Pfam:HPHLAWLY	243	535	1.4e-130	PFAM
Pfam:HPHLAWLY	527	890	9.1e-133	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (90/90)

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110065P20Rik	C	T	4: 124,850,602 (GRCm38)	G20R	not run	Het
4931409K22Rik	G	T	5: 24,545,422 (GRCm38)	Q626K	possibly damaging	Het
Adamts13	A	T	2: 26,996,549 (GRCm38)	M927L	probably benign	Het
Akap6	T	A	12: 53,140,961 (GRCm38)	N1719K	probably benign	Het
Ank3	G	A	10: 69,986,904 (GRCm38)	V468M		Het
Aox1	A	G	1: 58,085,467 (GRCm38)	T956A	probably benign	Het
Ap4m1	T	C	5: 138,174,817 (GRCm38)	V140A	probably benign	Het
Apc	A	G	18: 34,272,539 (GRCm38)	T173A	probably damaging	Het
Bclaf1	A	G	10: 20,334,619 (GRCm38)	T839A	possibly damaging	Het
Btn2a2	T	A	13: 23,482,806 (GRCm38)	I210F	possibly damaging	Het
Cadps	A	G	14: 12,376,706 (GRCm38)	W1269R	probably damaging	Het
Capn2	G	C	1: 182,492,146 (GRCm38)	P159R	probably damaging	Het
Ccdc134	T	G	15: 82,131,523 (GRCm38)	V68G	probably damaging	Het
Cdh26	A	G	2: 178,470,035 (GRCm38)	T463A	probably damaging	Het
Cfap206	C	T	4: 34,716,347 (GRCm38)	V373I	probably benign	Het
Clpx	T	C	9: 65,324,301 (GRCm38)		probably null	Het
Ctnnd2	A	T	15: 31,020,728 (GRCm38)	Y1145F	probably benign	Het
Ctr9	T	A	7: 111,033,927 (GRCm38)	D127E	probably benign	Het
Cyp2f2	G	A	7: 27,129,253 (GRCm38)	V183I	probably benign	Het
Cyp7b1	G	A	3: 18,097,302 (GRCm38)	S249L	probably benign	Het
D630023F18Rik	T	A	1: 65,116,691 (GRCm38)	D125V	possibly damaging	Het
Dbh	A	T	2: 27,174,848 (GRCm38)	Y357F	probably damaging	Het
Dbp	T	A	7: 45,706,990 (GRCm38)	L175Q	probably benign	Het
Dmwd	A	T	7: 19,080,843 (GRCm38)	T473S	probably benign	Het
Dnah9	T	A	11: 66,005,660 (GRCm38)	Q2446L	probably damaging	Het
Dnmbp	T	C	19: 43,854,176 (GRCm38)	K498R	probably benign	Het
Dopey2	T	A	16: 93,799,971 (GRCm38)	I1924N	probably damaging	Het
Dsg1a	T	A	18: 20,338,515 (GRCm38)		probably null	Het
Dzank1	T	C	2: 144,522,564 (GRCm38)	T38A	probably damaging	Het
Egfem1	A	G	3: 29,686,791 (GRCm38)	H538R	probably damaging	Het
Entpd1	T	C	19: 40,727,447 (GRCm38)	C353R	probably damaging	Het
Ephx2	C	A	14: 66,110,229 (GRCm38)	C78F	probably benign	Het
Faf2	A	T	13: 54,660,961 (GRCm38)	H388L	probably benign	Het
Fam186a	G	A	15: 99,944,664 (GRCm38)	T1233I	possibly damaging	Het
Gfra2	C	A	14: 70,895,970 (GRCm38)	A80E	probably damaging	Het
Gif	C	A	19: 11,750,187 (GRCm38)	P125Q	probably benign	Het
Gm17175	T	A	14: 51,574,035 (GRCm38)		probably benign	Het
Gm8180	T	C	14: 43,783,646 (GRCm38)	D35G	possibly damaging	Het
Gm884	T	A	11: 103,614,173 (GRCm38)	H2323L	possibly damaging	Het
Gpr68	C	T	12: 100,879,043 (GRCm38)	V81M	probably damaging	Het
Hsd3b6	A	G	3: 98,810,943 (GRCm38)	L35P	probably damaging	Het
Hspd1	A	C	1: 55,078,644 (GRCm38)	V485G	possibly damaging	Het
Il16	A	G	7: 83,670,140 (GRCm38)	V381A	possibly damaging	Het
Il17rd	G	A	14: 27,100,117 (GRCm38)	C600Y	probably benign	Het
Ins2	C	A	7: 142,679,586 (GRCm38)		probably benign	Het
Itsn2	T	C	12: 4,658,561 (GRCm38)	I872T	probably benign	Het
Jakmip3	A	T	7: 139,019,129 (GRCm38)	D219V	probably damaging	Het
Jph4	T	C	14: 55,109,735 (GRCm38)	T452A	probably damaging	Het
Kazn	T	C	4: 142,210,170 (GRCm38)	E12G	unknown	Het
Kif20b	A	C	19: 34,950,955 (GRCm38)	S1206R	possibly damaging	Het
Lca5l	A	G	16: 96,162,557 (GRCm38)		probably null	Het
Lgals2	A	T	15: 78,851,333 (GRCm38)	S60R	probably benign	Het
Map2k7	G	T	8: 4,243,744 (GRCm38)	R128L	probably benign	Het
Mapk8	G	C	14: 33,410,877 (GRCm38)	S34*	probably null	Het
Mcat	A	G	15: 83,547,909 (GRCm38)	Y253H	probably damaging	Het
Mmp2	T	C	8: 92,850,170 (GRCm38)	L607P	probably benign	Het
Ncor1	A	T	11: 62,333,926 (GRCm38)	V836E	probably damaging	Het
Neb	A	G	2: 52,137,380 (GRCm38)	S7113P	probably damaging	Het
Nphp4	G	T	4: 152,544,403 (GRCm38)	D749Y	probably damaging	Het
Nphp4	T	C	4: 152,524,272 (GRCm38)	F446L	possibly damaging	Het
Olfr115	T	A	17: 37,610,656 (GRCm38)	T32S	probably benign	Het
Olfr1185-ps1	A	G	2: 88,499,072 (GRCm38)		probably benign	Het
Olfr175-ps1	T	C	16: 58,824,002 (GRCm38)	K236E	probably damaging	Het
Olfr338	T	C	2: 36,376,809 (GRCm38)	I11T	possibly damaging	Het
Osbpl11	A	T	16: 33,210,061 (GRCm38)	R220*	probably null	Het
Plrg1	G	A	3: 83,056,837 (GRCm38)	V26M	probably damaging	Het
Polr1b	C	A	2: 129,125,544 (GRCm38)	F952L	probably damaging	Het
Ppfia3	A	C	7: 45,352,262 (GRCm38)	S560A	probably benign	Het
Ppp3cc	T	A	14: 70,225,015 (GRCm38)	N391I	possibly damaging	Het
Prdx6b	T	C	2: 80,292,960 (GRCm38)	S38P	possibly damaging	Het
Psg23	A	T	7: 18,606,914 (GRCm38)	*472R	probably null	Het
Rapgef4	A	T	2: 72,223,117 (GRCm38)	M587L	probably benign	Het
Rfx3	T	A	19: 27,826,070 (GRCm38)	M247L	probably benign	Het
Rgs7	T	A	1: 175,076,069 (GRCm38)	H463L	probably benign	Het
Riiad1	G	T	3: 94,465,855 (GRCm38)	S106R	probably benign	Het
Rims4	A	C	2: 163,918,628 (GRCm38)	I19S	probably benign	Het
Ros1	A	T	10: 52,096,137 (GRCm38)	M1648K	probably benign	Het
Rrn3	T	C	16: 13,811,589 (GRCm38)	F590L	probably benign	Het
Samd9l	T	A	6: 3,374,793 (GRCm38)	T823S	possibly damaging	Het
Sfxn1	C	A	13: 54,091,231 (GRCm38)	P115Q	possibly damaging	Het
Slc10a7	T	C	8: 78,698,573 (GRCm38)		probably null	Het
Slc25a16	A	T	10: 62,937,420 (GRCm38)	M142L	probably benign	Het
Stat2	CGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCAGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCTAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCTAGCCCCACAAGTCCAGCTGGAGCCAGC	CGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCAGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCTAGCCCCACAAGTCCTGCTGGAGCCAGCCCCACAAGTCCTGCTGGAGCTAGCCCCACAAGTCCAGCTGGAGCCAGC	10: 128,290,728 (GRCm38)		probably benign	Het
Stat5a	T	G	11: 100,875,027 (GRCm38)	L313R	probably damaging	Het
Strap	A	G	6: 137,741,978 (GRCm38)	E176G	possibly damaging	Het
Tlr4	T	G	4: 66,841,079 (GRCm38)	L703R	probably damaging	Het
Trim36	A	T	18: 46,167,624 (GRCm38)	V660D	possibly damaging	Het
Tspoap1	T	A	11: 87,775,524 (GRCm38)	D980E	probably damaging	Het
Usp28	T	C	9: 49,003,918 (GRCm38)	V157A	probably benign	Het
Vmn2r17	A	T	5: 109,427,873 (GRCm38)	R203S	probably benign	Het
Vmn2r66	T	C	7: 85,007,264 (GRCm38)	I181M	probably benign	Het
Zan	T	A	5: 137,463,579 (GRCm38)	T1113S	unknown	Het
Zfp442	C	T	2: 150,409,482 (GRCm38)	D167N	possibly damaging	Het

Other mutations in Map10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00813:Map10	APN	8	125,671,932 (GRCm38)	missense	probably benign	0.00
IGL01567:Map10	APN	8	125,671,493 (GRCm38)	missense	probably benign	0.32
IGL02566:Map10	APN	8	125,671,755 (GRCm38)	missense	probably benign
IGL03088:Map10	APN	8	125,671,070 (GRCm38)	missense	probably benign	0.14
debauched	UTSW	8	125,671,245 (GRCm38)	missense	probably damaging	1.00
R1083:Map10	UTSW	8	125,670,439 (GRCm38)	nonsense	probably null
R1543:Map10	UTSW	8	125,670,872 (GRCm38)	missense	probably benign	0.00
R3155:Map10	UTSW	8	125,671,574 (GRCm38)	missense	possibly damaging	0.79
R4076:Map10	UTSW	8	125,671,845 (GRCm38)	missense	probably benign	0.23
R4559:Map10	UTSW	8	125,671,814 (GRCm38)	missense	probably benign
R4856:Map10	UTSW	8	125,670,692 (GRCm38)	missense	probably damaging	1.00
R4886:Map10	UTSW	8	125,670,692 (GRCm38)	missense	probably damaging	1.00
R5412:Map10	UTSW	8	125,670,985 (GRCm38)	missense	probably damaging	1.00
R6034:Map10	UTSW	8	125,672,466 (GRCm38)	missense	probably damaging	1.00
R6034:Map10	UTSW	8	125,672,466 (GRCm38)	missense	probably damaging	1.00
R6150:Map10	UTSW	8	125,671,589 (GRCm38)	missense	probably damaging	0.96
R6351:Map10	UTSW	8	125,671,245 (GRCm38)	missense	probably damaging	1.00
R6466:Map10	UTSW	8	125,672,384 (GRCm38)	nonsense	probably null
R6544:Map10	UTSW	8	125,671,374 (GRCm38)	missense	probably benign	0.00
R6557:Map10	UTSW	8	125,670,252 (GRCm38)	missense	probably damaging	0.98
R6821:Map10	UTSW	8	125,670,399 (GRCm38)	missense	probably benign	0.01
R7096:Map10	UTSW	8	125,671,923 (GRCm38)	missense	probably damaging	0.99
R7128:Map10	UTSW	8	125,671,853 (GRCm38)	missense	probably benign
R7177:Map10	UTSW	8	125,671,845 (GRCm38)	missense	probably benign	0.23
R7237:Map10	UTSW	8	125,671,224 (GRCm38)	missense	probably benign	0.03
R7819:Map10	UTSW	8	125,670,521 (GRCm38)	frame shift	probably null
R8202:Map10	UTSW	8	125,670,908 (GRCm38)	missense	possibly damaging	0.95
R8812:Map10	UTSW	8	125,669,925 (GRCm38)	missense	probably damaging	0.99
R8859:Map10	UTSW	8	125,670,552 (GRCm38)	missense	probably benign	0.04
R8947:Map10	UTSW	8	125,671,100 (GRCm38)	missense	probably benign	0.06
R9178:Map10	UTSW	8	125,670,910 (GRCm38)	missense	probably damaging	0.98
R9698:Map10	UTSW	8	125,671,984 (GRCm38)	missense	probably benign	0.16
Z1088:Map10	UTSW	8	125,671,931 (GRCm38)	frame shift	probably null
Z1177:Map10	UTSW	8	125,670,070 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAGAAAGCCAGATGTCACC -3'
(R):5'- CAACAGTAGGTTCCTGTAGGG -3'

Sequencing Primer
(F):5'- GATGTCACCACAGCTGCC -3'
(R):5'- TCTTTCTGGGGTGACAGA -3'

Posted On

2019-11-26