Incidental Mutation 'R7815:Gpr155'
| ID |
601443 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr155
|
| Ensembl Gene |
ENSMUSG00000041762 |
| Gene Name |
G protein-coupled receptor 155 |
| Synonyms |
DEPDC3, 1110017O10Rik, PGR22, F730029F15Rik |
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R7815 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
73171850-73216842 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 73192904 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Serine
at position 516
(T516S)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000107675
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000076463]
[ENSMUST00000112043]
[ENSMUST00000112044]
|
| AlphaFold |
A2AWR3 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000076463
AA Change: T544S
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: T544S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112043
AA Change: T544S
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: T544S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112044
AA Change: T516S
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: T516S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
8.3e-11 |
PFAM |
|
transmembrane domain
|
382 |
404 |
N/A |
INTRINSIC |
|
transmembrane domain
|
414 |
436 |
N/A |
INTRINSIC |
|
transmembrane domain
|
449 |
471 |
N/A |
INTRINSIC |
|
transmembrane domain
|
491 |
510 |
N/A |
INTRINSIC |
|
transmembrane domain
|
629 |
651 |
N/A |
INTRINSIC |
|
transmembrane domain
|
661 |
683 |
N/A |
INTRINSIC |
|
DEP
|
731 |
805 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000131430
|
| SMART Domains |
Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
98 |
120 |
N/A |
INTRINSIC |
|
transmembrane domain
|
130 |
152 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.7%
- 20x: 99.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 61 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcc2 |
A |
G |
19: 43,818,866 (GRCm39) |
D1285G |
probably benign |
Het |
| Abcc9 |
A |
G |
6: 142,598,331 (GRCm39) |
V669A |
probably damaging |
Het |
| Ajap1 |
C |
A |
4: 153,517,288 (GRCm39) |
G18C |
probably damaging |
Het |
| Alms1 |
G |
T |
6: 85,592,340 (GRCm39) |
M397I |
probably benign |
Het |
| Ampd2 |
A |
T |
3: 107,982,247 (GRCm39) |
M794K |
probably benign |
Het |
| Ankrd35 |
A |
T |
3: 96,592,117 (GRCm39) |
Q801L |
probably damaging |
Het |
| Casz1 |
A |
T |
4: 149,013,762 (GRCm39) |
I109F |
possibly damaging |
Het |
| Ces5a |
G |
A |
8: 94,247,623 (GRCm39) |
S297F |
possibly damaging |
Het |
| Col7a1 |
A |
G |
9: 108,798,633 (GRCm39) |
D1762G |
probably damaging |
Het |
| Dsp |
A |
T |
13: 38,375,446 (GRCm39) |
Y1077F |
probably benign |
Het |
| Eef2k |
A |
G |
7: 120,457,793 (GRCm39) |
N51D |
probably benign |
Het |
| Erbb4 |
A |
G |
1: 68,081,885 (GRCm39) |
Y1050H |
probably damaging |
Het |
| Fpgt |
A |
T |
3: 154,792,289 (GRCm39) |
S579R |
probably benign |
Het |
| Gabra4 |
T |
A |
5: 71,815,152 (GRCm39) |
M1L |
possibly damaging |
Het |
| Galk2 |
G |
A |
2: 125,817,321 (GRCm39) |
S370N |
probably damaging |
Het |
| Homez |
T |
C |
14: 55,095,675 (GRCm39) |
N11S |
probably benign |
Het |
| Hspg2 |
G |
A |
4: 137,239,775 (GRCm39) |
G494D |
probably damaging |
Het |
| Htr3b |
C |
T |
9: 48,856,833 (GRCm39) |
R215Q |
probably benign |
Het |
| Igfbp4 |
A |
G |
11: 98,932,654 (GRCm39) |
E111G |
probably benign |
Het |
| Igkv8-19 |
T |
C |
6: 70,318,420 (GRCm39) |
T5A |
probably benign |
Het |
| Insrr |
A |
G |
3: 87,716,002 (GRCm39) |
K566E |
probably damaging |
Het |
| Iqsec3 |
A |
G |
6: 121,450,317 (GRCm39) |
V69A |
probably benign |
Het |
| Kdm5d |
T |
C |
Y: 940,702 (GRCm39) |
V1071A |
probably damaging |
Het |
| Kif28 |
A |
G |
1: 179,563,548 (GRCm39) |
C143R |
probably damaging |
Het |
| Kpna6 |
T |
C |
4: 129,551,590 (GRCm39) |
M65V |
probably benign |
Het |
| L3mbtl3 |
T |
C |
10: 26,156,276 (GRCm39) |
S669G |
unknown |
Het |
| Man1a2 |
A |
T |
3: 100,563,495 (GRCm39) |
I50N |
probably damaging |
Het |
| Map3k11 |
A |
T |
19: 5,745,667 (GRCm39) |
M356L |
possibly damaging |
Het |
| Meioc |
A |
T |
11: 102,566,414 (GRCm39) |
N677Y |
probably damaging |
Het |
| Myom3 |
A |
T |
4: 135,528,674 (GRCm39) |
K1108M |
possibly damaging |
Het |
| Nav1 |
C |
T |
1: 135,512,377 (GRCm39) |
E228K |
possibly damaging |
Het |
| Ncapd3 |
G |
A |
9: 26,974,736 (GRCm39) |
W736* |
probably null |
Het |
| Nfkb1 |
T |
C |
3: 135,309,552 (GRCm39) |
D514G |
probably damaging |
Het |
| Nnt |
T |
A |
13: 119,494,111 (GRCm39) |
T657S |
possibly damaging |
Het |
| Or4k5 |
A |
G |
14: 50,386,253 (GRCm39) |
F26S |
probably benign |
Het |
| Or51b4 |
A |
G |
7: 103,530,992 (GRCm39) |
S153P |
probably benign |
Het |
| Oser1 |
A |
T |
2: 163,253,336 (GRCm39) |
S58T |
possibly damaging |
Het |
| Pcdha5 |
T |
A |
18: 37,094,556 (GRCm39) |
I355N |
possibly damaging |
Het |
| Pllp |
C |
G |
8: 95,402,804 (GRCm39) |
G99A |
probably damaging |
Het |
| Pram1 |
A |
G |
17: 33,861,106 (GRCm39) |
N509S |
probably benign |
Het |
| Prkd1 |
A |
C |
12: 50,472,300 (GRCm39) |
N204K |
probably damaging |
Het |
| Prss23 |
G |
A |
7: 89,159,253 (GRCm39) |
P272L |
probably damaging |
Het |
| Pxdn |
T |
C |
12: 30,055,824 (GRCm39) |
F1057L |
probably damaging |
Het |
| Rapgef5 |
T |
A |
12: 117,719,702 (GRCm39) |
S777R |
probably benign |
Het |
| Repin1 |
G |
T |
6: 48,574,279 (GRCm39) |
E403* |
probably null |
Het |
| Rfx3 |
A |
T |
19: 27,803,448 (GRCm39) |
M265K |
probably benign |
Het |
| S100a7a |
C |
A |
3: 90,565,119 (GRCm39) |
H105N |
probably benign |
Het |
| Serpinb3b |
T |
C |
1: 107,084,625 (GRCm39) |
D132G |
probably damaging |
Het |
| Sp7 |
T |
A |
15: 102,274,822 (GRCm39) |
|
probably benign |
Het |
| Tacstd2 |
A |
G |
6: 67,512,140 (GRCm39) |
F184S |
possibly damaging |
Het |
| Trdc |
C |
A |
14: 54,381,259 (GRCm39) |
|
probably benign |
Het |
| Trpa1 |
T |
A |
1: 14,974,486 (GRCm39) |
H232L |
probably benign |
Het |
| Trpc3 |
G |
T |
3: 36,709,294 (GRCm39) |
T394K |
probably benign |
Het |
| Tuba3b |
G |
A |
6: 145,566,847 (GRCm39) |
V363I |
possibly damaging |
Het |
| Tubb2a |
C |
A |
13: 34,258,962 (GRCm39) |
R276L |
probably benign |
Het |
| Ubap2l |
A |
T |
3: 89,951,071 (GRCm39) |
L72* |
probably null |
Het |
| Vps13a |
A |
T |
19: 16,702,936 (GRCm39) |
D672E |
probably damaging |
Het |
| Wdr26 |
A |
G |
1: 181,036,638 (GRCm39) |
V166A |
probably benign |
Het |
| Wwox |
T |
C |
8: 115,438,776 (GRCm39) |
S281P |
probably damaging |
Het |
| Xirp2 |
G |
A |
2: 67,339,756 (GRCm39) |
G666R |
probably damaging |
Het |
| Zfp131 |
T |
C |
13: 120,237,516 (GRCm39) |
K247E |
probably damaging |
Het |
|
| Other mutations in Gpr155 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00731:Gpr155
|
APN |
2 |
73,192,957 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01432:Gpr155
|
APN |
2 |
73,182,229 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
IGL01528:Gpr155
|
APN |
2 |
73,192,767 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01718:Gpr155
|
APN |
2 |
73,212,576 (GRCm39) |
missense |
probably benign |
|
|
IGL01733:Gpr155
|
APN |
2 |
73,183,956 (GRCm39) |
splice site |
probably null |
|
|
IGL03342:Gpr155
|
APN |
2 |
73,180,022 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT1430001:Gpr155
|
UTSW |
2 |
73,200,482 (GRCm39) |
missense |
probably benign |
0.08 |
|
PIT4810001:Gpr155
|
UTSW |
2 |
73,178,607 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0226:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0399:Gpr155
|
UTSW |
2 |
73,200,346 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R0445:Gpr155
|
UTSW |
2 |
73,200,488 (GRCm39) |
splice site |
probably benign |
|
|
R1570:Gpr155
|
UTSW |
2 |
73,200,382 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1598:Gpr155
|
UTSW |
2 |
73,200,434 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1647:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1648:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1756:Gpr155
|
UTSW |
2 |
73,197,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1760:Gpr155
|
UTSW |
2 |
73,212,279 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2033:Gpr155
|
UTSW |
2 |
73,178,526 (GRCm39) |
missense |
probably benign |
0.18 |
|
R2044:Gpr155
|
UTSW |
2 |
73,203,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2145:Gpr155
|
UTSW |
2 |
73,187,002 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2172:Gpr155
|
UTSW |
2 |
73,212,471 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2274:Gpr155
|
UTSW |
2 |
73,178,479 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3878:Gpr155
|
UTSW |
2 |
73,198,736 (GRCm39) |
nonsense |
probably null |
|
|
R3924:Gpr155
|
UTSW |
2 |
73,200,420 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Gpr155
|
UTSW |
2 |
73,197,882 (GRCm39) |
nonsense |
probably null |
|
|
R4950:Gpr155
|
UTSW |
2 |
73,212,529 (GRCm39) |
missense |
probably benign |
|
|
R5337:Gpr155
|
UTSW |
2 |
73,178,592 (GRCm39) |
missense |
probably benign |
0.32 |
|
R5830:Gpr155
|
UTSW |
2 |
73,200,433 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R5887:Gpr155
|
UTSW |
2 |
73,174,062 (GRCm39) |
nonsense |
probably null |
|
|
R5929:Gpr155
|
UTSW |
2 |
73,204,011 (GRCm39) |
nonsense |
probably null |
|
|
R6293:Gpr155
|
UTSW |
2 |
73,204,341 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6553:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6585:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7003:Gpr155
|
UTSW |
2 |
73,173,961 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7353:Gpr155
|
UTSW |
2 |
73,197,835 (GRCm39) |
nonsense |
probably null |
|
|
R7506:Gpr155
|
UTSW |
2 |
73,198,683 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7631:Gpr155
|
UTSW |
2 |
73,213,291 (GRCm39) |
intron |
probably benign |
|
|
R7753:Gpr155
|
UTSW |
2 |
73,212,550 (GRCm39) |
missense |
probably benign |
0.27 |
|
R7810:Gpr155
|
UTSW |
2 |
73,212,296 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7813:Gpr155
|
UTSW |
2 |
73,212,329 (GRCm39) |
nonsense |
probably null |
|
|
R7873:Gpr155
|
UTSW |
2 |
73,173,934 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R8506:Gpr155
|
UTSW |
2 |
73,173,806 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8680:Gpr155
|
UTSW |
2 |
73,174,039 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8856:Gpr155
|
UTSW |
2 |
73,203,993 (GRCm39) |
missense |
probably benign |
0.27 |
|
R8872:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9116:Gpr155
|
UTSW |
2 |
73,204,109 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R9683:Gpr155
|
UTSW |
2 |
73,192,780 (GRCm39) |
missense |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- AGATACATGTGACAGTTTCTGTAAGTT -3'
(R):5'- TACCCATTTGGAAGAAACATTGTT -3'
Sequencing Primer
(F):5'- GTTCTCCTCAAACGCAGT -3'
(R):5'- CAGATCCCCTGGACTGGAGTTATAG -3'
|
| Posted On |
2019-12-03 |
Incidental Mutation