Mutagenetix > Incidental Mutations

Incidental Mutation 'R7820:Tas2r125'

601810

Institutional Source

Beutler Lab

Gene Symbol

Tas2r125

Ensembl Gene

ENSMUSG00000059410

Gene Name

taste receptor, type 2, member 125

Synonyms

mGR25, Tas2r25, mt2r59, T2R26

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R7820 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

132909651-132910587 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 132909878 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 76 (I76M)

Ref Sequence

ENSEMBL: ENSMUSP00000075483 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076119]

Predicted Effect

probably benign
Transcript: ENSMUST00000076119
AA Change: I76M

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000075483
Gene: ENSMUSG00000059410
AA Change: I76M

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	1.1e-90	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

96% (49/51)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933405O20Rik	T	A	7: 50,599,623	I135N	probably benign	Het
Abca14	A	T	7: 120,212,721	N175I	probably benign	Het
Adam12	A	G	7: 133,998,188	V99A	probably benign	Het
Ankfn1	G	T	11: 89,421,130	P730T	probably damaging	Het
Arhgap21	A	G	2: 20,863,172	S847P	probably damaging	Het
Cacna1i	C	T	15: 80,372,372	A989V	probably benign	Het
Cacnb2	T	A	2: 14,960,666	N152K	probably damaging	Het
Chd3	A	T	11: 69,353,238	Y1334N	probably damaging	Het
Clca4a	A	T	3: 144,960,671	D473E	probably damaging	Het
Crhr2	A	C	6: 55,102,779	I191S	probably damaging	Het
E430018J23Rik	A	T	7: 127,391,436	W460R	possibly damaging	Het
Eef1akmt3	T	C	10: 127,033,194	Q137R	possibly damaging	Het
Eml1	T	C	12: 108,515,174	I432T	possibly damaging	Het
Fcgbp	A	G	7: 28,120,359	T2504A	probably benign	Het
Hmgxb4	G	T	8: 75,000,946	E186*	probably null	Het
Ighv16-1	T	C	12: 114,068,969	N71S	probably benign	Het
Kif2b	A	G	11: 91,577,274	V61A	probably benign	Het
Map1b	C	T	13: 99,431,177	G1679R	unknown	Het
Mast4	G	A	13: 102,754,088	S1086L	probably damaging	Het
Mcm8	A	G	2: 132,840,772	E724G	possibly damaging	Het
Mfap5	C	A	6: 122,520,921	D51E	probably damaging	Het
Mon1a	T	C	9: 107,901,312	L245P	probably damaging	Het
Mtx1	G	T	3: 89,214,008	H106Q	probably benign	Het
Naip1	G	A	13: 100,423,070	S1142F	probably benign	Het
Ngp	A	G	9: 110,420,864	T77A	probably benign	Het
Oca2	A	T	7: 56,331,965	I612F	probably damaging	Het
Odf3	A	C	7: 140,849,263	T128P	probably benign	Het
Olfr1224-ps1	T	A	2: 89,156,248	D309V	probably benign	Het
Olfr147	A	T	9: 38,403,566	I228F	probably damaging	Het
Olfr96	A	T	17: 37,225,895	M257L	probably benign	Het
Otop3	T	A	11: 115,339,588	V97D	probably damaging	Het
Ovgp1	A	G	3: 105,986,521		probably benign	Het
Pdc	T	C	1: 150,333,270	I168T	probably damaging	Het
Peg10	CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG	CCACATCAGGATCCACATCAGGATGCACATCAG	6: 4,756,398		probably benign	Het
Plekha7	A	T	7: 116,237,480	F18I	probably benign	Het
Plppr4	A	G	3: 117,321,949	I753T	possibly damaging	Het
Pms2	T	G	5: 143,914,633	S123A	possibly damaging	Het
Pou4f2	T	A	8: 78,436,502		probably benign	Het
Ptch1	A	T	13: 63,523,061	L885Q	probably damaging	Het
Repin1	G	T	6: 48,597,345	E403*	probably null	Het
Rgs11	T	A	17: 26,205,195		probably null	Het
Samd3	T	A	10: 26,233,518		probably null	Het
Slit3	A	G	11: 35,700,408	D1349G	probably benign	Het
Sorbs2	A	T	8: 45,796,556	Q868L	probably null	Het
Speer4f1	T	A	5: 17,479,530	S185R	probably damaging	Het
Spin2f	A	G	X: 31,254,699	K28R	probably benign	Het
Tex15	A	T	8: 33,575,062	I1507F	probably damaging	Het
Tmem2	C	T	19: 21,807,461	A436V	probably damaging	Het
Vmn1r215	A	T	13: 23,076,545	I252F	probably damaging	Het
Vmn1r71	A	G	7: 10,748,725	F12S	possibly damaging	Het
Zfp128	A	G	7: 12,891,022	Y439C	probably benign	Het

Other mutations in Tas2r125

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03252:Tas2r125	APN	6	132910590	unclassified	probably null
R0090:Tas2r125	UTSW	6	132910398	missense	probably benign	0.21
R0725:Tas2r125	UTSW	6	132910122	missense	probably benign	0.44
R1901:Tas2r125	UTSW	6	132910176	missense	probably benign	0.13
R1902:Tas2r125	UTSW	6	132910176	missense	probably benign	0.13
R2679:Tas2r125	UTSW	6	132910227	missense	probably benign	0.43
R4770:Tas2r125	UTSW	6	132909787	missense	probably damaging	1.00
R4784:Tas2r125	UTSW	6	132909903	missense	probably benign	0.16
R5795:Tas2r125	UTSW	6	132909658	missense	probably damaging	1.00
R7535:Tas2r125	UTSW	6	132910324	missense	probably damaging	1.00
Z1177:Tas2r125	UTSW	6	132909656	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCCATAGATATCTTATGGGCAGC -3'
(R):5'- AGATTAGCAATGTCACTGAAACCAC -3'

Sequencing Primer
(F):5'- GGGCAGCTATTATCATTGTGCAATTC -3'
(R):5'- TGTCACTGAAACCACCTTCTTAAATC -3'

Posted On

2019-12-03