Mutagenetix > Incidental Mutation

Incidental Mutation 'R7825:Ugt2b35'

602126

Institutional Source

Beutler Lab

Gene Symbol

Ugt2b35

Ensembl Gene

ENSMUSG00000035811

Gene Name

UDP glucuronosyltransferase 2 family, polypeptide B35

Synonyms

MMRRC Submission

045879-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R7825 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87148719-87161133 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 87149218 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 156 (C156*)

Ref Sequence

ENSEMBL: ENSMUSP00000031186 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031186]

AlphaFold

Q8BJL9

Predicted Effect

probably null
Transcript: ENSMUST00000031186
AA Change: C156*

SMART Domains

Protein: ENSMUSP00000031186
Gene: ENSMUSG00000035811
AA Change: C156*

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:UDPGT	24	526	1e-253	PFAM
Pfam:Glyco_tran_28_C	338	449	1.9e-8	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

98% (55/56)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700013G24Rik	C	T	4: 137,182,654 (GRCm39)	R270C	probably damaging	Het
Adprs	C	A	4: 126,215,489 (GRCm39)		probably benign	Het
Akp3	TCACCACCACCACCACCACCACCACCACCAC	TCACCACCACCACCACCACCACCACCAC	1: 87,055,489 (GRCm39)		probably benign	Het
Ambra1	A	G	2: 91,598,106 (GRCm39)	H89R	probably damaging	Het
Atp1a1	T	C	3: 101,493,485 (GRCm39)	N540D	probably benign	Het
Cd38	A	T	5: 44,058,797 (GRCm39)	H137L	probably damaging	Het
Clasp1	T	C	1: 118,473,123 (GRCm39)	V3A	probably benign	Het
Cmya5	G	T	13: 93,234,136 (GRCm39)	H317Q	possibly damaging	Het
Cpeb2	A	T	5: 43,394,882 (GRCm39)	D95V	probably damaging	Het
Cyp2f2	G	A	7: 26,828,678 (GRCm39)	V183I	probably benign	Het
Dcst1	A	G	3: 89,260,128 (GRCm39)	L572P	possibly damaging	Het
Eif3e	A	C	15: 43,129,667 (GRCm39)		probably null	Het
Ephb4	T	C	5: 137,370,699 (GRCm39)	S911P	probably damaging	Het
Fam186b	T	C	15: 99,181,728 (GRCm39)	T157A	not run	Het
Gnmt	T	C	17: 47,040,019 (GRCm39)	D20G	probably damaging	Het
Gpatch8	A	C	11: 102,372,268 (GRCm39)	D423E	unknown	Het
Gtf3c2	T	C	5: 31,315,715 (GRCm39)	H790R	probably damaging	Het
Hepacam	T	C	9: 37,296,064 (GRCm39)	I405T	probably benign	Het
Hspg2	T	A	4: 137,286,160 (GRCm39)	V3574E	probably damaging	Het
Ifi202b	A	C	1: 173,802,616 (GRCm39)	F73V	probably damaging	Het
Kcnb1	A	T	2: 166,947,892 (GRCm39)	S319T	probably damaging	Het
Krt16	T	A	11: 100,139,460 (GRCm39)	D86V	unknown	Het
Krt76	T	A	15: 101,795,938 (GRCm39)	T411S	possibly damaging	Het
Myh8	A	T	11: 67,194,538 (GRCm39)	D1583V	possibly damaging	Het
Nav1	A	T	1: 135,377,782 (GRCm39)	V1689D	probably damaging	Het
Ncor2	T	C	5: 125,114,141 (GRCm39)	T611A	possibly damaging	Het
Nipbl	T	A	15: 8,320,971 (GRCm39)	Y2712F	probably damaging	Het
Ntng2	T	A	2: 29,094,090 (GRCm39)	H427L	probably benign	Het
Pcdhga4	T	A	18: 37,820,374 (GRCm39)	L641Q	probably damaging	Het
Phc1	T	C	6: 122,299,340 (GRCm39)	K623R	probably benign	Het
Polr1b	C	A	2: 128,967,464 (GRCm39)	F952L	probably damaging	Het
Prcc	T	A	3: 87,777,052 (GRCm39)	E307D	possibly damaging	Het
Prss27	T	C	17: 24,261,932 (GRCm39)	F80S	probably damaging	Het
Rsf1	G	GACGGCGGCC	7: 97,229,116 (GRCm39)		probably benign	Het
Serpina11	T	C	12: 103,950,836 (GRCm39)	Q295R	probably benign	Het
Sgk2	G	T	2: 162,848,801 (GRCm39)	V284L	possibly damaging	Het
Slc15a2	C	T	16: 36,573,396 (GRCm39)	V603I	possibly damaging	Het
Smpd3	A	C	8: 106,982,254 (GRCm39)	C617G	probably benign	Het
Stat1	A	G	1: 52,190,467 (GRCm39)	H574R	probably damaging	Het
Ston1	T	C	17: 88,943,881 (GRCm39)	V429A	possibly damaging	Het
Tep1	T	C	14: 51,081,344 (GRCm39)		probably null	Het
Tesk1	A	C	4: 43,447,143 (GRCm39)	R510S	probably damaging	Het
Themis	A	G	10: 28,658,470 (GRCm39)	E499G	probably benign	Het
Thra	G	A	11: 98,653,774 (GRCm39)	V202I	probably benign	Het
Tiam1	A	T	16: 89,694,977 (GRCm39)	M160K	probably benign	Het
Tmc1	T	C	19: 20,782,009 (GRCm39)	I570V	possibly damaging	Het
Treml1	C	A	17: 48,673,784 (GRCm39)	P270Q	probably damaging	Het
Ttc39d	A	G	17: 80,523,575 (GRCm39)	N78S	probably damaging	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,376,118 (GRCm39)		probably null	Het
Ttll3	AAGTA	AAGTAGAGTA	6: 113,376,120 (GRCm39)		probably null	Het
Ube3b	T	A	5: 114,539,373 (GRCm39)	L388Q	probably damaging	Het
Vmn1r35	A	T	6: 66,656,443 (GRCm39)	C76S	probably damaging	Het
Wwc2	A	G	8: 48,443,197 (GRCm39)	L12P	probably damaging	Het
Zfat	T	C	15: 68,051,769 (GRCm39)	E668G	probably benign	Het
Zfp40	T	C	17: 23,395,301 (GRCm39)	T429A	probably benign	Het

Other mutations in Ugt2b35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00703:Ugt2b35	APN	5	87,156,051 (GRCm39)	missense	probably benign	0.00
IGL01109:Ugt2b35	APN	5	87,156,165 (GRCm39)	missense	probably damaging	1.00
IGL01586:Ugt2b35	APN	5	87,159,250 (GRCm39)	missense	probably benign	0.07
IGL02151:Ugt2b35	APN	5	87,151,141 (GRCm39)	missense	possibly damaging	0.65
IGL02225:Ugt2b35	APN	5	87,155,264 (GRCm39)	splice site	probably benign
IGL02483:Ugt2b35	APN	5	87,149,004 (GRCm39)	missense	possibly damaging	0.87
IGL02504:Ugt2b35	APN	5	87,149,400 (GRCm39)	missense	possibly damaging	0.50
IGL02690:Ugt2b35	APN	5	87,149,096 (GRCm39)	missense	probably benign	0.01
IGL02954:Ugt2b35	APN	5	87,159,180 (GRCm39)	missense	probably benign	0.00
IGL03242:Ugt2b35	APN	5	87,155,254 (GRCm39)	missense	probably damaging	1.00
R0329:Ugt2b35	UTSW	5	87,151,264 (GRCm39)	missense	probably null	0.38
R0513:Ugt2b35	UTSW	5	87,151,271 (GRCm39)	splice site	probably benign
R0571:Ugt2b35	UTSW	5	87,148,793 (GRCm39)	missense	possibly damaging	0.51
R0827:Ugt2b35	UTSW	5	87,155,989 (GRCm39)	splice site	probably benign
R1396:Ugt2b35	UTSW	5	87,159,389 (GRCm39)	missense	possibly damaging	0.56
R1437:Ugt2b35	UTSW	5	87,148,890 (GRCm39)	missense	probably benign	0.02
R1557:Ugt2b35	UTSW	5	87,155,156 (GRCm39)	splice site	probably null
R1869:Ugt2b35	UTSW	5	87,149,147 (GRCm39)	missense	probably damaging	1.00
R1937:Ugt2b35	UTSW	5	87,149,141 (GRCm39)	missense	probably damaging	1.00
R2067:Ugt2b35	UTSW	5	87,149,412 (GRCm39)	missense	probably damaging	1.00
R2219:Ugt2b35	UTSW	5	87,151,191 (GRCm39)	missense	possibly damaging	0.81
R3055:Ugt2b35	UTSW	5	87,149,457 (GRCm39)	missense	probably benign	0.05
R3793:Ugt2b35	UTSW	5	87,149,465 (GRCm39)	missense	probably benign	0.15
R4452:Ugt2b35	UTSW	5	87,151,237 (GRCm39)	missense	probably damaging	0.99
R4548:Ugt2b35	UTSW	5	87,156,134 (GRCm39)	nonsense	probably null
R4902:Ugt2b35	UTSW	5	87,151,159 (GRCm39)	missense	possibly damaging	0.89
R5311:Ugt2b35	UTSW	5	87,159,139 (GRCm39)	nonsense	probably null
R6187:Ugt2b35	UTSW	5	87,155,181 (GRCm39)	missense	probably damaging	0.98
R6332:Ugt2b35	UTSW	5	87,149,415 (GRCm39)	missense	probably damaging	1.00
R6719:Ugt2b35	UTSW	5	87,155,247 (GRCm39)	missense	probably damaging	1.00
R7211:Ugt2b35	UTSW	5	87,149,177 (GRCm39)	missense	probably benign	0.39
R7652:Ugt2b35	UTSW	5	87,149,369 (GRCm39)	missense	probably damaging	0.99
R7766:Ugt2b35	UTSW	5	87,149,061 (GRCm39)	missense	possibly damaging	0.94
R8188:Ugt2b35	UTSW	5	87,149,302 (GRCm39)	missense	probably damaging	0.98
R8189:Ugt2b35	UTSW	5	87,149,302 (GRCm39)	missense	probably damaging	0.98
R8191:Ugt2b35	UTSW	5	87,149,302 (GRCm39)	missense	probably damaging	0.98
R8192:Ugt2b35	UTSW	5	87,149,302 (GRCm39)	missense	probably damaging	0.98
R8193:Ugt2b35	UTSW	5	87,149,302 (GRCm39)	missense	probably damaging	0.98
R8220:Ugt2b35	UTSW	5	87,149,302 (GRCm39)	missense	probably damaging	0.98
R8288:Ugt2b35	UTSW	5	87,149,316 (GRCm39)	missense	probably damaging	1.00
R8695:Ugt2b35	UTSW	5	87,156,064 (GRCm39)	missense	probably damaging	0.97
R8898:Ugt2b35	UTSW	5	87,159,189 (GRCm39)	missense	possibly damaging	0.53
R8924:Ugt2b35	UTSW	5	87,152,780 (GRCm39)	missense	possibly damaging	0.73
R8944:Ugt2b35	UTSW	5	87,149,310 (GRCm39)	missense	probably benign	0.21
R9284:Ugt2b35	UTSW	5	87,156,140 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CTTTTGAGGTCCCAAGAGATACATG -3'
(R):5'- TCCTTCTCTGTAAATGTCTGGAAC -3'

Sequencing Primer
(F):5'- GAGGTCCCAAGAGATACATGCTTATC -3'
(R):5'- CTGTAAATGTCTGGAACCAAAAGTC -3'

Posted On

2019-12-03