Mutagenetix > Incidental Mutation

Incidental Mutation 'R7826:Or8g33'

602196

Institutional Source

Beutler Lab

Gene Symbol

Or8g33

Ensembl Gene

ENSMUSG00000058856

Gene Name

olfactory receptor family 8 subfamily G member 33

Synonyms

GA_x6K02T2PVTD-33124064-33123120, MOR171-21, Olfr952

MMRRC Submission

045880-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7826 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

39337389-39338444 bp(-) (GRCm39)

Type of Mutation

makesense

DNA Base Change (assembly)

A to G at 39337423 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Glutamine at position 315 (*315Q)

Ref Sequence

ENSEMBL: ENSMUSP00000073558 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073895]

AlphaFold

Q8VFD7

Predicted Effect

probably null
Transcript: ENSMUST00000073895
AA Change: *315Q

SMART Domains

Protein: ENSMUSP00000073558
Gene: ENSMUSG00000058856
AA Change: *315Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.5e-42	PFAM
Pfam:7tm_1	41	294	3.2e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (60/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ajm1	C	A	2: 25,468,477 (GRCm39)	R478L	possibly damaging	Het
Arhgap27	T	C	11: 103,229,153 (GRCm39)	T521A	probably benign	Het
Bean1	CT	C	8: 104,908,664 (GRCm39)		probably null	Het
Btbd9	A	T	17: 30,553,301 (GRCm39)	I387N	probably benign	Het
Ccdc27	C	T	4: 154,123,958 (GRCm39)		probably null	Het
Ccdc88a	A	T	11: 29,453,563 (GRCm39)	H1642L	probably benign	Het
Cd300c2	C	T	11: 114,891,644 (GRCm39)	V77I	possibly damaging	Het
Chrnb2	A	T	3: 89,670,550 (GRCm39)	S63T	probably damaging	Het
Dlgap2	C	T	8: 14,793,410 (GRCm39)	P467L	probably benign	Het
Dnah5	C	T	15: 28,367,958 (GRCm39)	T2781M	probably damaging	Het
Donson	T	C	16: 91,485,344 (GRCm39)	D86G	possibly damaging	Het
Echs1	T	A	7: 139,696,349 (GRCm39)		probably benign	Het
Epha8	C	T	4: 136,663,498 (GRCm39)	M483I	probably benign	Het
Eprs1	T	G	1: 185,139,165 (GRCm39)	V850G	probably damaging	Het
Fbxw5	C	A	2: 25,392,561 (GRCm39)	C114*	probably null	Het
Gm5160	C	T	18: 14,558,129 (GRCm39)	R69C	probably benign	Het
Gpr75	A	G	11: 30,841,209 (GRCm39)	H38R	probably damaging	Het
Igsf9	T	C	1: 172,319,197 (GRCm39)	V230A	probably benign	Het
Lonp2	C	T	8: 87,435,641 (GRCm39)	P626S	probably damaging	Het
Lrrtm1	G	T	6: 77,221,095 (GRCm39)		probably null	Het
Man1a2	T	C	3: 100,489,455 (GRCm39)	E508G	probably damaging	Het
Mipep	C	A	14: 61,039,580 (GRCm39)	A203E	probably damaging	Het
Mrc1	T	A	2: 14,299,668 (GRCm39)	C753S	probably damaging	Het
Mthfr	A	G	4: 148,139,467 (GRCm39)	E568G	probably benign	Het
Naca	T	A	10: 127,879,479 (GRCm39)		probably benign	Het
Nek10	G	A	14: 14,860,846 (GRCm38)		probably null	Het
Nf2	T	C	11: 4,739,750 (GRCm39)	T419A	probably benign	Het
Npas3	A	G	12: 53,878,539 (GRCm39)	T164A	possibly damaging	Het
Nudcd3	T	A	11: 6,100,581 (GRCm39)	I124F	possibly damaging	Het
Pappa2	A	T	1: 158,764,010 (GRCm39)	C500*	probably null	Het
Pcdhb18	T	A	18: 37,623,995 (GRCm39)	S442T	probably damaging	Het
Phactr4	T	C	4: 132,105,752 (GRCm39)	N105D	possibly damaging	Het
Polr1b	C	A	2: 128,967,464 (GRCm39)	F952L	probably damaging	Het
Ptprb	GAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACT	GAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACTGCAAAGACCCTCGGGAGCACT	10: 116,119,582 (GRCm39)		probably benign	Het
Pzp	T	C	6: 128,464,496 (GRCm39)	T1344A	probably benign	Het
Ranbp9	T	C	13: 43,573,097 (GRCm39)	I312V	possibly damaging	Het
Rsf1	A	G	7: 97,310,368 (GRCm39)		probably benign	Het
Slc6a4	A	T	11: 76,903,851 (GRCm39)	I150L	probably benign	Het
Smpd5	T	C	15: 76,180,496 (GRCm39)	S433P	probably benign	Het
Spats1	C	T	17: 45,763,644 (GRCm39)	D209N	probably damaging	Het
Spcs2	C	T	7: 99,488,984 (GRCm39)	G235R	probably damaging	Het
Sptlc3	T	C	2: 139,389,115 (GRCm39)	M165T	probably benign	Het
Synm	T	C	7: 67,385,337 (GRCm39)	D775G	probably damaging	Het
Tango6	A	G	8: 107,419,245 (GRCm39)	D264G	probably benign	Het
Tbc1d30	A	T	10: 121,132,710 (GRCm39)	L218H	probably damaging	Het
Tmem132d	A	G	5: 127,866,953 (GRCm39)	I649T	probably damaging	Het
Tmem145	T	C	7: 25,006,939 (GRCm39)	Y114H	probably damaging	Het
Triml1	A	T	8: 43,591,803 (GRCm39)	V185E	possibly damaging	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,376,118 (GRCm39)		probably null	Het
Ttn	T	G	2: 76,715,220 (GRCm39)		probably benign	Het
Utrn	C	T	10: 12,277,050 (GRCm39)		probably null	Het
Uty	T	C	Y: 1,137,716 (GRCm39)	K887E	possibly damaging	Het
Zfand2b	T	C	1: 75,145,502 (GRCm39)	F3L	possibly damaging	Het
Zfp280d	C	A	9: 72,219,953 (GRCm39)	Q243K	possibly damaging	Het
Zfp418	T	C	7: 7,185,668 (GRCm39)	F544L	probably benign	Het

Other mutations in Or8g33

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00976:Or8g33	APN	9	39,337,953 (GRCm39)	missense	probably benign	0.02
IGL01368:Or8g33	APN	9	39,337,476 (GRCm39)	missense	probably damaging	1.00
IGL01866:Or8g33	APN	9	39,338,025 (GRCm39)	missense	probably damaging	0.99
IGL01939:Or8g33	APN	9	39,337,634 (GRCm39)	missense	probably damaging	1.00
IGL03048:Or8g33	UTSW	9	39,338,065 (GRCm39)	missense	probably benign	0.00
R0069:Or8g33	UTSW	9	39,338,188 (GRCm39)	missense	probably damaging	0.98
R1460:Or8g33	UTSW	9	39,337,503 (GRCm39)	missense	probably benign	0.43
R1478:Or8g33	UTSW	9	39,337,888 (GRCm39)	missense	possibly damaging	0.91
R1737:Or8g33	UTSW	9	39,338,254 (GRCm39)	missense	probably damaging	0.99
R2112:Or8g33	UTSW	9	39,337,966 (GRCm39)	missense	probably benign	0.26
R2169:Or8g33	UTSW	9	39,337,654 (GRCm39)	missense	possibly damaging	0.95
R2225:Or8g33	UTSW	9	39,337,915 (GRCm39)	missense	probably benign	0.00
R3731:Or8g33	UTSW	9	39,338,365 (GRCm39)	start codon destroyed	probably benign	0.23
R3777:Or8g33	UTSW	9	39,337,901 (GRCm39)	missense	possibly damaging	0.75
R3802:Or8g33	UTSW	9	39,337,582 (GRCm39)	missense	probably damaging	1.00
R4600:Or8g33	UTSW	9	39,337,731 (GRCm39)	missense	probably benign	0.27
R4887:Or8g33	UTSW	9	39,337,531 (GRCm39)	missense	possibly damaging	0.80
R5215:Or8g33	UTSW	9	39,337,919 (GRCm39)	nonsense	probably null
R5813:Or8g33	UTSW	9	39,338,032 (GRCm39)	missense	probably damaging	1.00
R6140:Or8g33	UTSW	9	39,337,543 (GRCm39)	missense	possibly damaging	0.91
R6180:Or8g33	UTSW	9	39,338,008 (GRCm39)	missense	probably damaging	1.00
R6383:Or8g33	UTSW	9	39,337,530 (GRCm39)	missense	probably damaging	1.00
R6416:Or8g33	UTSW	9	39,338,187 (GRCm39)	missense	probably damaging	0.98
R6808:Or8g33	UTSW	9	39,337,836 (GRCm39)	missense	probably damaging	0.99
R7099:Or8g33	UTSW	9	39,337,599 (GRCm39)	missense	probably benign	0.01
R8309:Or8g33	UTSW	9	39,337,966 (GRCm39)	missense	probably benign	0.40
R8870:Or8g33	UTSW	9	39,337,548 (GRCm39)	missense	probably damaging	0.97
R8879:Or8g33	UTSW	9	39,337,515 (GRCm39)	missense	possibly damaging	0.86
R9084:Or8g33	UTSW	9	39,337,521 (GRCm39)	missense	probably damaging	1.00
R9209:Or8g33	UTSW	9	39,337,635 (GRCm39)	missense	probably damaging	0.99
R9435:Or8g33	UTSW	9	39,337,506 (GRCm39)	missense	probably benign
R9583:Or8g33	UTSW	9	39,337,851 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- GTGAATGAACTCAGCTTTTACCAG -3'
(R):5'- AGCTCCCATATCTTGGCTGTTG -3'

Sequencing Primer
(F):5'- TATGCGTCTGAGAGTCCT -3'
(R):5'- TGGTTCTACAACATTCATGTATCTAC -3'

Posted On

2019-12-03